##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934079.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400294
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.939949137384023	31.0	30.0	31.0	26.0	34.0
2	30.21752262087366	31.0	30.0	33.0	26.0	34.0
3	30.22291116029718	31.0	30.0	33.0	26.0	34.0
4	33.96238015058932	35.0	33.0	37.0	28.0	37.0
5	34.38835705756269	35.0	35.0	37.0	32.0	37.0
6	33.89067785177894	35.0	35.0	37.0	30.0	37.0
7	34.476392351621556	35.0	35.0	37.0	32.0	37.0
8	34.68185883375719	35.0	35.0	37.0	32.0	37.0
9	35.99793152033256	37.0	35.0	39.0	31.0	39.0
10	35.094787830944256	37.0	34.0	39.0	30.0	39.0
11	35.28095100101425	37.0	34.0	39.0	30.0	39.0
12	35.37407755299854	37.0	34.0	39.0	30.0	39.0
13	35.432529590750796	37.0	35.0	39.0	30.0	39.0
14	36.05869685780951	38.0	34.0	40.0	29.0	41.0
15	36.301365995992946	38.0	35.0	40.0	30.0	41.0
16	36.464546058646896	38.0	35.0	40.0	31.0	41.0
17	36.227030632485125	38.0	35.0	40.0	30.0	41.0
18	36.36318056228672	38.0	35.0	40.0	30.0	41.0
19	36.36308313389659	38.0	35.0	40.0	30.0	41.0
20	36.39849460646425	38.0	35.0	40.0	30.0	41.0
21	36.09157269406986	38.0	34.0	40.0	30.0	41.0
22	36.3115085412222	38.0	35.0	40.0	30.0	41.0
23	36.31340214942018	38.0	35.0	40.0	30.0	41.0
24	36.217879858304144	38.0	34.0	40.0	30.0	41.0
25	35.67088439996603	38.0	34.0	40.0	29.0	41.0
26	35.80054160192259	38.0	34.0	40.0	29.0	41.0
27	35.84223845473577	38.0	34.0	40.0	29.0	41.0
28	35.737158188731286	38.0	34.0	40.0	29.0	41.0
29	35.925502255841955	38.0	34.0	40.0	30.0	41.0
30	35.47432137379026	38.0	34.0	40.0	27.0	41.0
31	35.583096424128264	38.0	34.0	40.0	29.0	41.0
32	35.655280868561604	38.0	34.0	40.0	29.0	41.0
33	35.66044207507482	38.0	34.0	40.0	29.0	41.0
34	35.65516845118837	38.0	34.0	40.0	29.0	41.0
35	35.73372820976582	38.0	34.0	40.0	29.0	41.0
36	35.55850699735694	38.0	34.0	40.0	28.0	41.0
37	35.61745367155141	38.0	34.0	40.0	29.0	41.0
38	35.5177394614958	38.0	34.0	40.0	28.0	41.0
39	35.46669198139367	38.0	34.0	40.0	28.0	41.0
40	35.403405996592504	38.0	34.0	40.0	28.0	41.0
41	35.299057692595945	38.0	34.0	40.0	27.0	41.0
42	35.53266599049699	38.0	34.0	40.0	28.0	41.0
43	35.51903351036988	38.0	34.0	40.0	28.0	41.0
44	35.45209521002064	38.0	34.0	40.0	28.0	41.0
45	35.540322862695916	38.0	34.0	40.0	28.0	41.0
46	35.428732381699454	38.0	34.0	40.0	28.0	41.0
47	35.39133486887138	38.0	34.0	40.0	28.0	41.0
48	35.3917695493812	38.0	34.0	40.0	28.0	41.0
49	35.45916751187877	38.0	34.0	40.0	28.0	41.0
50	35.30417143399601	37.0	34.0	40.0	28.0	41.0
51	34.14586029268488	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	8.0
13	4.0
14	8.0
15	16.0
16	25.0
17	71.0
18	153.0
19	280.0
20	462.0
21	824.0
22	1287.0
23	1827.0
24	2437.0
25	3466.0
26	4385.0
27	5752.0
28	7726.0
29	10065.0
30	12779.0
31	15992.0
32	20697.0
33	26885.0
34	35063.0
35	39181.0
36	48315.0
37	61328.0
38	71287.0
39	29965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.069309057842485	5.0235576851014505	58.154006804998325	12.753126452057737
2	19.433716218579345	4.96585010017637	54.96984716233568	20.630586518908604
3	20.31831603771228	5.145967713730408	53.57162485573104	20.964091392826273
4	18.793936456704323	4.4277456069788705	49.96502570610601	26.813292230210795
5	17.980784123669103	5.451243336147931	52.588597380925016	23.979375159257945
6	20.37702288817719	7.0495685671031785	54.9633519363268	17.61005660839283
7	83.69098712446352	1.8658785792442554	9.929701669273085	4.5134326270191405
8	85.90311121325826	2.8973704327319423	6.09976667149645	5.099751682513352
9	81.28400625540228	4.50418942077573	8.057327864019944	6.154476459802045
10	37.9121345810829	26.615687469709766	18.734979789854457	16.737198159352875
11	27.637936116954037	27.7568487161936	24.24118273069294	20.364032436159423
12	22.91365846103114	23.116009732846358	31.49135385491664	22.478977951205863
13	22.683577570485696	25.95567258065322	32.39893678146562	18.961813067395465
14	20.817948807626394	28.628458083308768	30.40715074420301	20.146442364861826
15	18.247088390033326	27.0383767930571	33.986270091482766	20.72826472542681
16	20.29908017607059	26.25170499682733	30.285490164728923	23.163724662373156
17	20.80995468330777	27.185518643796808	28.225254437988077	23.779272234907342
18	21.315832862845806	26.01587832942787	30.57277900742954	22.09550980029678
19	21.445737383023477	29.143079836320307	27.87501186627829	21.536170914377934
20	23.636627079096865	27.856525453791463	29.021669073231177	19.4851783938805
21	22.569911115330232	29.043652915107398	28.853792462540035	19.53264350702234
22	21.71129220023283	26.183505123734058	28.29745137324067	23.80775130279245
23	20.062254243131296	28.40587168431203	26.907972640109517	24.623901432447152
24	21.526927708134522	27.607208701604318	27.849780411397624	23.016083178863536
25	19.789204934373235	29.476334893853018	26.388854192168754	24.34560597960499
26	19.29157069553878	29.127841036837925	27.67341004361794	23.907178224005357
27	20.04101985040995	28.352161161546263	29.277481051427202	22.329337936616586
28	17.463164574038082	26.947693445317693	30.288737777733367	25.30040420291086
29	18.494906243910727	24.858229201536872	31.42240453266849	25.224460021883914
30	19.63781620508926	26.351881367195112	31.004461720635334	23.005840707080296
31	19.668043987669062	27.279699420925617	27.58847247273254	25.463784118672777
32	18.271820212144075	28.921992335633313	29.004181926284183	23.802005525938437
33	19.315802884879613	26.592704362293713	28.38388784243581	25.707604910390863
34	17.511379136334792	26.530500082439406	28.16329997451873	27.794820806707072
35	17.233083683492634	25.376598200322764	29.649457648628257	27.740860467556345
36	19.249601542865992	26.255702058986646	28.768854891654634	25.72584150649273
37	16.816889586154176	26.848016707719825	30.998965760166282	25.336127945959717
38	17.769189645610474	27.8702653549641	29.36216880592764	24.99837619349778
39	18.044986934603067	25.73733306020075	31.314234037982082	24.903445967214097
40	19.301563350937062	24.63664206807996	30.56203690287639	25.499757678106594
41	17.38946874047575	24.80426886238615	28.9057542706111	28.900508126527
42	18.43844774091043	24.640389313854318	29.25899463894038	27.66216830629487
43	18.76345885773956	24.80476849515606	29.301213607998122	27.130559039106256
44	17.985031002213372	25.21621608118033	29.230765387440233	27.56798752916606
45	19.180652220617848	24.022593393855516	28.35565859093566	28.441095794590975
46	19.662298210815052	24.656127746106613	30.12560767835641	25.555966364721925
47	16.263796109859253	24.41105787246374	32.387195411372645	26.937950606304366
48	16.937550900088436	24.25542226463549	31.67696742893973	27.130059406336343
49	18.545618970057006	22.406531199568317	33.02697517324766	26.020874657127013
50	18.684267063708173	22.763268997286996	30.332455645100854	28.22000829390398
51	17.24882211574493	22.662343177764342	29.181051926833778	30.90778277965695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42.0
1	125.0
2	208.0
3	407.0
4	606.0
5	465.0
6	324.0
7	372.5
8	421.0
9	557.5
10	694.0
11	844.5
12	995.0
13	1005.5
14	1016.0
15	997.5
16	979.0
17	948.0
18	917.0
19	940.5
20	964.0
21	1059.5
22	1155.0
23	1501.0
24	1847.0
25	2149.5
26	2864.5
27	3277.0
28	3885.0
29	4493.0
30	5064.0
31	5635.0
32	7236.0
33	8837.0
34	9893.5
35	10950.0
36	12834.0
37	14718.0
38	15852.5
39	16987.0
40	19102.5
41	21218.0
42	24964.0
43	28710.0
44	30999.5
45	33289.0
46	39940.5
47	46592.0
48	48713.0
49	50834.0
50	47043.0
51	43252.0
52	36735.5
53	30219.0
54	25863.0
55	21507.0
56	17920.5
57	14334.0
58	12768.0
59	11202.0
60	9245.5
61	7289.0
62	6298.0
63	5307.0
64	4539.0
65	3771.0
66	3001.0
67	2231.0
68	1817.0
69	1403.0
70	1048.5
71	694.0
72	555.5
73	417.0
74	349.0
75	201.0
76	121.0
77	81.5
78	42.0
79	36.0
80	30.0
81	30.0
82	30.0
83	15.5
84	1.0
85	1.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	400294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.21250757140731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.29199741849155	32.535560085129454
2	10.764854925355554	9.303527499340563
3	3.65887174985546	4.743270695803122
4	1.9468807398547578	3.365183948464032
5	1.2688046985659096	2.7414116321708266
6	0.9315769547274836	2.415346572570597
7	0.7270599365228522	2.1992658108302465
8	0.6412974529865713	2.2169656834165146
9	0.5311131819773733	2.065565915772433
>10	4.102141667869674	30.044913706843563
>50	0.09046205792194086	2.700300124472488
>100	0.04027020642976722	3.1615730232919685
>500	0.0011672523602831077	0.3339121476418767
>1k	0.003501757080849323	2.173203154252305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1621	0.4049523600153887	RNA PCR Primer, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1608	0.401704747010947	RNA PCR Primer, Index 35 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1573	0.39296117353744997	RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	1459	0.3644821056523455	TruSeq Adapter, Index 10 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	1268	0.3167671761255477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	1088	0.27180022683327754	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	788	0.19685531134616055	RNA PCR Primer, Index 10 (95% over 21bp)
TCTCCGGGGTAGAAATAATGAACTTCACTGGTCATGATAATGCATACCTAA	536	0.13390158233698232	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT	492	0.12290966139887183	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	443	0.11066865853597606	RNA PCR Primer, Index 35 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39845713400650523	0.0
2	0.0	0.0	0.0	1.7512128585489666	0.0
3	0.0	0.0	0.0	2.3152982557820003	0.0
4	0.0	0.0	0.0	3.2616027219993304	0.0
5	0.0	0.0	0.0	4.8501851139412535	0.0
6	0.0	0.0	0.0	5.65934038481716	0.0
7	0.0	0.0	0.0	6.451757957900943	0.0
8	0.0	0.0	0.0	7.699840617146397	0.0
9	2.4981638495705656E-4	0.0	0.0	8.153257355843454	0.0
10	2.4981638495705656E-4	0.0	0.0	9.461046131093646	0.0
11	2.4981638495705656E-4	0.0	0.0	11.574992380600259	0.0
12	2.4981638495705656E-4	0.0	0.0	13.699430918275068	0.0
13	2.4981638495705656E-4	0.0	0.0	14.299989507711832	0.0
14	2.4981638495705656E-4	0.0	0.0	14.494846287978335	0.0
15	2.4981638495705656E-4	0.0	0.0	14.83309767321019	0.0
16	2.4981638495705656E-4	0.0	0.0	15.598035443948698	0.0
17	2.4981638495705656E-4	0.0	0.0	16.781415659490275	0.0
18	2.4981638495705656E-4	0.0	0.0	18.076463799107657	0.0
19	2.4981638495705656E-4	0.0	0.0	19.074480257011096	0.0
20	2.4981638495705656E-4	0.0	0.0	19.940343847272256	0.0
21	2.4981638495705656E-4	0.0	0.0	21.217405207172728	0.0
22	2.4981638495705656E-4	0.0	0.0	22.554172683077937	0.0
23	2.4981638495705656E-4	0.0	0.0	24.00710477798818	0.0
24	2.4981638495705656E-4	0.0	0.0	25.041844244480306	0.0
25	2.4981638495705656E-4	0.0	0.0	25.976657156989614	0.0
26	2.4981638495705656E-4	0.0	0.0	26.768824913688437	0.0
27	2.4981638495705656E-4	0.0	0.0	27.569486427475805	0.0
28	2.4981638495705656E-4	0.0	0.0	28.462330187312325	0.0
29	2.4981638495705656E-4	0.0	0.0	29.359420825693114	0.0
30	2.4981638495705656E-4	0.0	0.0	30.28598979749884	0.0
31	2.4981638495705656E-4	0.0	0.0	31.1803324556451	0.0
32	2.4981638495705656E-4	0.0	0.0	32.02920853172918	0.0
33	2.4981638495705656E-4	0.0	0.0	32.856350582321994	0.0
34	2.4981638495705656E-4	0.0	0.0	33.66100915826867	0.0
35	2.4981638495705656E-4	0.0	0.0	34.55310346895032	0.0
36	2.4981638495705656E-4	0.0	0.0	35.3595107595917	0.0
37	2.4981638495705656E-4	0.0	0.0	36.227123064547555	0.0
38	2.4981638495705656E-4	0.0	0.0	37.117468660534506	0.0
39	2.4981638495705656E-4	0.0	0.0	38.08225953923866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGG	25	3.8862338E-5	45.000004	2
TCGACGG	35	1.2092823E-7	45.000004	2
GCCCGAC	35	1.2092823E-7	45.000004	9
TTTAGCG	20	7.0277706E-4	45.0	1
CGGGTCG	20	7.0277706E-4	45.0	6
ATCGTTC	20	7.0277706E-4	45.0	37
GTAGCGG	20	7.0277706E-4	45.0	2
TACGATA	20	7.0277706E-4	45.0	17
TCCGTTG	45	3.8380676E-10	45.0	1
TAAGGTT	20	7.0277706E-4	45.0	12
TTCGATG	20	7.0277706E-4	45.0	1
GACTAGA	20	7.0277706E-4	45.0	12
TTCAGCG	30	2.1619435E-6	44.999996	1
CGGGTGC	85	0.0	42.35294	6
TCTCCCG	55	6.002665E-11	40.909092	1
GCCGTTG	55	6.002665E-11	40.909092	1
TTGGGTC	375	0.0	40.8	5
GTTCGCG	50	1.0786607E-9	40.500004	1
GGCACCG	145	0.0	40.344826	8
GCACCGA	140	0.0	40.178574	9
>>END_MODULE