SRR2934078.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171697
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	5047	2.93948059663244	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	4235	2.4665544534849184	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	2475	1.4414928624262509	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	1918	1.117084165710525	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	1011	0.5888279935001777	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	980	0.5707729313849398	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	714	0.41584885000902755	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	657	0.3826508325713321	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	535	0.31159542682749264	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	362	0.21083653179729406	TruSeq Adapter, Index 27 (95% over 23bp)
TTTCTCGGGATCTTAAGTACTGTAGAAAAGATGTCACTTCTTTAAGGCTGT	353	0.2055947395702895	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCTGTCGAATGC	334	0.19452873375772436	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCCACATTGT	331	0.1927814696820562	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT	322	0.18753967745505162	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	293	0.17064945805692588	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	293	0.17064945805692588	No Hit
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	277	0.16133071632002888	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG	265	0.15434166001735616	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	244	0.14211081148767887	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCACTTTG	237	0.13803386197778644	No Hit
TGTTTTTGGTTTTTGTTTTGTTTTCTTTTGTTTGTTTGTTTGTTTTTTACA	232	0.13512175518500613	No Hit
TGCCTGGGGGATTTGTGTTCAACATTATTTGAACCATTTGCCATTAACGAA	228	0.13279206975078192	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	217	0.12638543480666523	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT	217	0.12638543480666523	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	198	0.11531942899410007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGC	184	0.10716552997431521	No Hit
TTTTTGGGAAGAATTTTATACTTTTAACTTATGTTTGACTCTGATCTCTTA	172	0.1001764736716425	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGGC	70	0.0	45.000004	4
CCGCGTA	35	1.2038618E-7	45.000004	40
ACCCGTC	35	1.2038618E-7	45.000004	39
CGCGTAA	35	1.2038618E-7	45.000004	41
CACCGCG	70	0.0	45.000004	38
CTGTCGG	25	3.876906E-5	45.0	2
GTCGCCA	25	3.876906E-5	45.0	19
TCTTGCG	25	3.876906E-5	45.0	1
ACCGGTC	20	7.016509E-4	45.0	39
CGAACCC	20	7.016509E-4	45.0	34
ACTGCAC	20	7.016509E-4	45.0	24
GTCGAAG	25	3.876906E-5	45.0	43
GGATGTC	20	7.016509E-4	45.0	8
GGTCGAA	55	1.8189894E-12	45.0	42
CGACGGA	20	7.016509E-4	45.0	41
ACTGACG	25	3.876906E-5	45.0	43
GGCACCG	45	3.8016879E-10	45.0	8
CACATTG	55	1.8189894E-12	45.0	44
GGCAATT	20	7.016509E-4	45.0	8
GACCGGA	20	7.016509E-4	45.0	40

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content