##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	171697
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.390006814329894	30.0	28.0	31.0	25.0	33.0
2	28.41079343261676	31.0	28.0	31.0	16.0	34.0
3	28.64522385364916	31.0	28.0	33.0	16.0	34.0
4	33.38035609241862	35.0	32.0	37.0	28.0	37.0
5	34.24167574273284	35.0	35.0	37.0	32.0	37.0
6	32.851336948228564	35.0	32.0	37.0	26.0	37.0
7	34.18749308374637	35.0	33.0	35.0	32.0	37.0
8	33.017781324076715	35.0	32.0	37.0	26.0	37.0
9	35.3228070379797	37.0	34.0	39.0	30.0	39.0
10	35.076448627524066	37.0	34.0	39.0	30.0	39.0
11	35.21243236631974	37.0	34.0	39.0	30.0	39.0
12	35.090816962439646	37.0	34.0	39.0	30.0	39.0
13	34.670850393425624	37.0	33.0	39.0	27.0	39.0
14	35.44045615240802	38.0	33.0	40.0	27.0	41.0
15	35.626068015166254	38.0	33.0	40.0	27.0	41.0
16	36.21092971921466	38.0	34.0	40.0	30.0	41.0
17	35.62829868896952	37.0	34.0	40.0	29.0	41.0
18	35.918926946888995	38.0	34.0	40.0	30.0	41.0
19	35.760630645847044	38.0	34.0	40.0	29.0	41.0
20	35.27317308980355	37.0	33.0	40.0	27.0	41.0
21	35.53664886398714	37.0	34.0	40.0	29.0	41.0
22	35.724730193305646	38.0	34.0	40.0	29.0	41.0
23	35.25005678608246	37.0	33.0	40.0	27.0	41.0
24	35.30587022487288	37.0	33.0	40.0	27.0	41.0
25	35.29058748842438	37.0	34.0	40.0	27.0	41.0
26	35.25643429995865	37.0	34.0	40.0	27.0	41.0
27	34.81854662574186	37.0	33.0	40.0	26.0	41.0
28	34.698824091277075	37.0	33.0	40.0	25.0	41.0
29	34.95725609649557	37.0	33.0	40.0	26.0	41.0
30	34.621967768802016	37.0	33.0	40.0	25.0	41.0
31	34.858028969638376	37.0	33.0	40.0	25.0	41.0
32	34.35559153625282	37.0	33.0	40.0	24.0	41.0
33	34.19723699307501	37.0	33.0	40.0	23.0	41.0
34	34.315398638298866	37.0	33.0	40.0	24.0	41.0
35	34.07937238274402	37.0	33.0	40.0	23.0	41.0
36	33.9198529968491	37.0	32.0	40.0	23.0	41.0
37	34.30255042312912	37.0	33.0	40.0	24.0	41.0
38	33.801353547237284	36.0	32.0	40.0	22.0	41.0
39	33.64424538576679	36.0	32.0	40.0	21.0	41.0
40	33.88440100875379	36.0	32.0	40.0	23.0	41.0
41	33.69982585601379	36.0	32.0	40.0	23.0	41.0
42	33.53025970168378	36.0	33.0	40.0	21.0	41.0
43	33.42358340565065	36.0	33.0	40.0	19.0	41.0
44	33.71967477591338	37.0	33.0	40.0	20.0	41.0
45	33.57496054095296	37.0	33.0	40.0	20.0	41.0
46	33.57687670722261	36.0	33.0	40.0	20.0	41.0
47	33.28567185215816	36.0	32.0	39.0	20.0	41.0
48	33.526392423862966	36.0	33.0	40.0	20.0	41.0
49	33.57910738102588	36.0	33.0	40.0	20.0	41.0
50	33.17537289527482	36.0	32.0	39.0	18.0	41.0
51	32.47017129012155	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	7.0
15	7.0
16	20.0
17	51.0
18	109.0
19	337.0
20	826.0
21	1493.0
22	2282.0
23	2994.0
24	3258.0
25	2990.0
26	2895.0
27	2964.0
28	3693.0
29	4722.0
30	6173.0
31	7807.0
32	9780.0
33	12472.0
34	16759.0
35	16446.0
36	18061.0
37	21177.0
38	23365.0
39	11004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.57553131388434	3.691386570528314	43.120730123415086	13.612351992172256
2	16.003191669044885	23.364415219834942	43.65946988007944	16.972923231040728
3	17.914116146467325	22.796554395242783	42.188273528366835	17.101055929923064
4	16.99039587179741	4.161983028241611	57.10175483555333	21.74586626440765
5	32.86021304973296	5.026296324338806	40.44450398084999	21.66898664507825
6	17.544278583784227	22.971863224168157	43.600645322865276	15.883212869182339
7	69.0862391305614	1.8922869939486422	25.226998724497225	3.794475150992737
8	68.84919363762909	18.72484667757736	7.062441393850795	5.363518290942766
9	65.20556561850236	4.173631455412733	7.5842909311170255	23.036511994967878
10	48.52501790945677	21.682964757683592	14.715458045277435	15.076559287582194
11	40.13698550353239	19.6270173619807	22.657355690547885	17.578641443939034
12	26.764591111085224	16.70384456338783	26.314961822279948	30.216602503246996
13	28.736087409797488	22.860038323325394	32.10364770496864	16.300226561908477
14	18.48954844872071	35.000029121067925	25.730210778289663	20.780211651921697
15	14.604215565793229	20.17624070309906	38.02687292148378	27.19267080962393
16	16.124917732983103	20.27408749133648	29.328409931449006	34.27258484423141
17	17.597278927412827	28.11813834836951	30.024403454923497	24.260179269294163
18	17.68522455255479	20.862915484836662	29.88403990751149	31.567820055097062
19	20.279329283563488	24.60206060676657	25.590429652236207	29.528180457433734
20	29.486828540976255	21.24614873876655	32.36166036680897	16.905362353448226
21	22.530387834382662	34.52477329248618	24.55197236993075	18.392866503200406
22	19.438312841808536	20.364945223271228	35.43276819047508	24.763973744445156
23	25.158855425546168	27.971950587371943	26.135576043844676	20.733617943237213
24	20.80816787713239	27.60327786740595	26.5793811190644	25.009173136397255
25	17.52098172944198	36.00936533544558	24.401707659423284	22.067945275689148
26	16.802273772983803	24.38772954681794	33.58358037705958	25.22641630313867
27	26.74304152081865	27.719179717758607	23.10465529391894	22.4331234675038
28	15.516869834650576	32.31856118627582	31.298158966085605	20.866410012987995
29	18.510515617628727	20.64276021130247	33.959824574686806	26.886899596381998
30	16.561151330541595	33.55038235962189	28.10823718527406	21.780229124562457
31	22.756367321502413	25.01965672085127	21.880988019592653	30.34298793805366
32	28.994682492996382	26.00977303039657	23.395283551838414	21.600260924768634
33	26.101213183689868	22.03532967961001	24.79717176188285	27.066285374817266
34	21.224016727141418	23.4179979848221	25.53160509502204	29.82638019301444
35	23.919462774538868	20.67246370058883	26.711590767456624	28.69648275741568
36	22.170451434795016	29.876468429850263	22.744136472972738	25.208943662381987
37	17.258892118091754	22.596201447899496	27.288187912427126	32.85671852158163
38	18.411503986674198	27.007460817603103	25.710990873457312	28.870044322265386
39	19.420840201051853	26.452413262899178	27.737234779873848	26.38951175617512
40	19.111574459658584	19.562951012539532	32.41815523858891	28.907319289212975
41	20.06266853818063	21.783723652713793	28.181622276452124	29.971985532653456
42	22.544948368346564	20.096448976976884	31.65518325888047	25.703419395796086
43	25.412791137876606	22.713268140969266	23.618933353523943	28.255007367630185
44	21.123840253469776	21.59967850341008	31.87825063920744	25.398230603912708
45	18.931023838506206	24.144277418941506	27.199659865926602	29.725038876625682
46	25.99404765371556	26.751777841196994	23.5478779477801	23.70629655730735
47	15.838948845932077	26.678975171377484	35.015172076390385	22.46690390630005
48	20.50064939981479	25.168174167283063	29.08670506764824	25.244471365253908
49	20.73711247138855	19.036442104404852	36.206806175996086	24.01963924821051
50	23.557196689517	23.346360157719705	27.207813764946387	25.88862938781691
51	18.94966132198	22.154143636755446	28.41284355579888	30.483351485465676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	36.0
1	88.0
2	140.0
3	197.0
4	254.0
5	211.0
6	168.0
7	181.5
8	195.0
9	231.5
10	268.0
11	245.5
12	223.0
13	271.0
14	319.0
15	297.5
16	276.0
17	302.0
18	328.0
19	343.0
20	358.0
21	561.5
22	765.0
23	660.5
24	556.0
25	767.0
26	1067.0
27	1156.0
28	1417.0
29	1678.0
30	1926.0
31	2174.0
32	2483.0
33	2792.0
34	3337.0
35	3882.0
36	4262.0
37	4642.0
38	4970.0
39	5298.0
40	5763.5
41	6229.0
42	7583.0
43	8937.0
44	9731.0
45	10525.0
46	19232.5
47	27940.0
48	23146.0
49	18352.0
50	17072.0
51	15792.0
52	13843.0
53	11894.0
54	11350.5
55	10807.0
56	9953.5
57	9100.0
58	7980.0
59	6860.0
60	6350.5
61	5841.0
62	4951.5
63	4062.0
64	3575.5
65	3089.0
66	2511.0
67	1933.0
68	1659.0
69	1385.0
70	1285.0
71	1185.0
72	899.0
73	613.0
74	471.5
75	218.0
76	106.0
77	133.5
78	161.0
79	107.0
80	53.0
81	30.5
82	8.0
83	6.5
84	5.0
85	3.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	171697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.83225682452227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05371461009011	24.970150905374002
2	8.607109912722628	5.6518168634280155
3	2.9855247285886612	2.940645439349552
4	1.5291279358546797	2.0081888443012983
5	1.0838714255304052	1.779297250388766
6	0.8532604839281912	1.680868040792792
7	0.6971546157666927	1.6022411573877238
8	0.6936067551266586	1.8218140095633588
9	0.6208756120059604	1.8346272794515923
>10	6.7161001915844745	39.77530183986907
>50	0.08337472504080039	1.823561273639027
>100	0.060313630880579006	3.8771789839076973
>500	0.007095721280068119	1.680868040792792
>1k	0.007095721280068119	5.613959475121871
>5k	0.0017739303200170298	2.93948059663244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	5047	2.93948059663244	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	4235	2.4665544534849184	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	2475	1.4414928624262509	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	1918	1.117084165710525	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	1011	0.5888279935001777	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	980	0.5707729313849398	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	714	0.41584885000902755	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	657	0.3826508325713321	No Hit
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	535	0.31159542682749264	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	362	0.21083653179729406	TruSeq Adapter, Index 27 (95% over 23bp)
TTTCTCGGGATCTTAAGTACTGTAGAAAAGATGTCACTTCTTTAAGGCTGT	353	0.2055947395702895	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCTGTCGAATGC	334	0.19452873375772436	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCCACATTGT	331	0.1927814696820562	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGT	322	0.18753967745505162	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	293	0.17064945805692588	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	293	0.17064945805692588	No Hit
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	277	0.16133071632002888	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATG	265	0.15434166001735616	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	244	0.14211081148767887	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCACTTTG	237	0.13803386197778644	No Hit
TGTTTTTGGTTTTTGTTTTGTTTTCTTTTGTTTGTTTGTTTGTTTTTTACA	232	0.13512175518500613	No Hit
TGCCTGGGGGATTTGTGTTCAACATTATTTGAACCATTTGCCATTAACGAA	228	0.13279206975078192	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	217	0.12638543480666523	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTAT	217	0.12638543480666523	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	198	0.11531942899410007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCCTTTGTCGTATGC	184	0.10716552997431521	No Hit
TTTTTGGGAAGAATTTTATACTTTTAACTTATGTTTGACTCTGATCTCTTA	172	0.1001764736716425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18870452017216374	0.0
2	0.0	0.0	0.0	1.2586125558396477	0.0
3	0.0	0.0	0.0	1.5218670099069873	0.0
4	0.0	0.0	0.0	2.238245280930942	0.0
5	0.0	0.0	0.0	4.528326062773374	0.0
6	0.0	0.0	0.0	4.88535035556824	0.0
7	0.0	0.0	0.0	5.626772745010105	0.0
8	0.0	0.0	0.0	6.579614087607821	0.0
9	0.0	0.0	0.0	6.810252945596021	0.0
10	0.0	0.0	0.0	10.031043058411038	0.0
11	0.0	0.0	0.0	10.876718871034438	0.0
12	0.0	0.0	0.0	15.308945409646062	0.0
13	0.0	0.0	0.0	15.77662976056658	0.0
14	0.0	0.0	0.0	16.035807265124028	0.0
15	0.0	0.0	0.0	16.92283499420491	0.0
16	0.0	0.0	0.0	17.51108056634653	0.0
17	0.0	0.0	0.0	18.148831953965416	0.0
18	0.0	0.0	0.0	18.755715009580832	0.0
19	0.0	0.0	0.0	20.567627856048738	0.0
20	0.0	0.0	0.0	21.126752360262557	0.0
21	0.0	0.0	0.0	21.698107713006053	0.0
22	0.0	0.0	0.0	22.31605677443403	0.0
23	0.0	0.0	0.0	22.945654263033134	0.0
24	0.0	0.0	0.0	23.434888204220226	0.0
25	0.0	0.0	0.0	23.8408358911338	0.0
26	0.0	0.0	0.0	24.235717572234808	0.0
27	0.0	0.0	0.0	24.65273126496095	0.0
28	0.0	0.0	0.0	25.040623889759285	0.0
29	0.0	0.0	0.0	25.466956324222323	0.0
30	0.0	0.0	0.0	25.929398882915834	0.0
31	0.0	0.0	0.0	26.448336313389284	0.0
32	0.0	0.0	0.0	26.8630203206812	0.0
33	0.0	0.0	0.0	27.29459454737124	0.0
34	0.0	0.0	0.0	27.710443397380267	0.0
35	0.0	0.0	0.0	28.196182810416023	0.0
36	0.0	0.0	0.0	28.644064835145635	0.0
37	0.0	0.0	0.0	29.0925292812338	0.0
38	0.0	0.0	0.0	29.52585077199951	0.0
39	0.0	0.0	0.0	29.962666790916558	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGGGC	70	0.0	45.000004	4
CCGCGTA	35	1.2038618E-7	45.000004	40
ACCCGTC	35	1.2038618E-7	45.000004	39
CGCGTAA	35	1.2038618E-7	45.000004	41
CACCGCG	70	0.0	45.000004	38
CTGTCGG	25	3.876906E-5	45.0	2
GTCGCCA	25	3.876906E-5	45.0	19
TCTTGCG	25	3.876906E-5	45.0	1
ACCGGTC	20	7.016509E-4	45.0	39
CGAACCC	20	7.016509E-4	45.0	34
ACTGCAC	20	7.016509E-4	45.0	24
GTCGAAG	25	3.876906E-5	45.0	43
GGATGTC	20	7.016509E-4	45.0	8
GGTCGAA	55	1.8189894E-12	45.0	42
CGACGGA	20	7.016509E-4	45.0	41
ACTGACG	25	3.876906E-5	45.0	43
GGCACCG	45	3.8016879E-10	45.0	8
CACATTG	55	1.8189894E-12	45.0	44
GGCAATT	20	7.016509E-4	45.0	8
GACCGGA	20	7.016509E-4	45.0	40
>>END_MODULE