##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934077.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498123
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19906930617538	31.0	30.0	31.0	27.0	34.0
2	30.537154477910075	31.0	30.0	33.0	27.0	34.0
3	30.594491721924104	31.0	31.0	34.0	27.0	34.0
4	34.13842765742598	35.0	33.0	37.0	30.0	37.0
5	34.52004424609986	35.0	35.0	37.0	32.0	37.0
6	34.08558528716803	35.0	35.0	37.0	30.0	37.0
7	34.4808812281304	35.0	35.0	37.0	32.0	37.0
8	34.69782162237038	35.0	35.0	37.0	32.0	37.0
9	36.02619232599177	38.0	35.0	39.0	31.0	39.0
10	35.400786552718905	37.0	34.0	39.0	30.0	39.0
11	35.538499527225206	37.0	35.0	39.0	30.0	39.0
12	35.56828935825087	37.0	35.0	39.0	30.0	39.0
13	35.60149802358052	37.0	35.0	39.0	30.0	39.0
14	36.378926490043625	38.0	35.0	40.0	30.0	41.0
15	36.61109404705264	38.0	35.0	40.0	31.0	41.0
16	36.71336999094601	38.0	35.0	40.0	31.0	41.0
17	36.472172535699016	38.0	35.0	40.0	30.0	41.0
18	36.559058706383766	38.0	35.0	40.0	31.0	41.0
19	36.5756369410768	38.0	35.0	40.0	31.0	41.0
20	36.641590530852824	38.0	35.0	40.0	31.0	41.0
21	36.31947330277863	38.0	35.0	40.0	30.0	41.0
22	36.50038444319977	38.0	35.0	40.0	30.0	41.0
23	36.601913583592804	38.0	35.0	40.0	31.0	41.0
24	36.5052587413149	38.0	35.0	40.0	30.0	41.0
25	36.02450800304342	38.0	34.0	40.0	30.0	41.0
26	36.139206581506976	38.0	34.0	40.0	30.0	41.0
27	36.20326706456036	38.0	35.0	40.0	30.0	41.0
28	36.09192508677576	38.0	35.0	40.0	30.0	41.0
29	36.17681175131443	38.0	35.0	40.0	30.0	41.0
30	35.75132246453185	38.0	34.0	40.0	29.0	41.0
31	35.9130335278636	38.0	34.0	40.0	30.0	41.0
32	35.995808264223896	38.0	34.0	40.0	30.0	41.0
33	36.01375764620385	38.0	34.0	40.0	30.0	41.0
34	35.93793902309269	38.0	34.0	40.0	29.0	41.0
35	35.95208613133704	38.0	34.0	40.0	29.0	41.0
36	35.761807826580984	38.0	34.0	40.0	29.0	41.0
37	35.84631105168803	38.0	34.0	40.0	29.0	41.0
38	35.73122702625657	38.0	34.0	40.0	29.0	41.0
39	35.668419245848916	38.0	34.0	40.0	29.0	41.0
40	35.58059555571616	38.0	34.0	40.0	29.0	41.0
41	35.46186785191609	38.0	34.0	40.0	28.0	41.0
42	35.675166575323765	38.0	34.0	40.0	29.0	41.0
43	35.625825348357736	38.0	34.0	40.0	29.0	41.0
44	35.544225020727815	38.0	34.0	40.0	29.0	41.0
45	35.638061282052824	38.0	34.0	40.0	29.0	41.0
46	35.54910333391552	37.0	34.0	40.0	29.0	41.0
47	35.51960058057949	37.0	34.0	40.0	29.0	41.0
48	35.488742740246884	37.0	34.0	40.0	29.0	41.0
49	35.45275162961759	37.0	34.0	40.0	29.0	41.0
50	35.281073148599845	37.0	34.0	40.0	28.0	41.0
51	34.14077446735043	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	4.0
12	8.0
13	7.0
14	25.0
15	28.0
16	44.0
17	92.0
18	201.0
19	347.0
20	513.0
21	892.0
22	1404.0
23	2039.0
24	2872.0
25	4053.0
26	5340.0
27	6807.0
28	8493.0
29	11076.0
30	14432.0
31	18349.0
32	23804.0
33	31608.0
34	44724.0
35	47035.0
36	57773.0
37	76023.0
38	92478.0
39	47644.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.47135747596477	4.792792141699941	55.55435103378081	17.181499348554475
2	20.395163443567153	3.95805855180347	54.287394880380944	21.359383124248428
3	21.6484683501866	3.7575056763088637	52.255768153648795	22.338257819855738
4	20.330922282247556	5.180246645908741	47.34694041431534	27.14189065752836
5	18.69076513230668	5.813825099423235	49.55302204475601	25.94238772351407
6	22.215998859719384	6.4733007710946895	52.952383246708145	18.358317122477782
7	85.28114541990632	2.267311487323412	8.366206740102344	4.085336352667915
8	87.44506878823101	2.859333939609293	5.61768880376935	4.077908468390337
9	81.57342664362015	5.5893825420629035	7.6258273558940255	5.21136345842292
10	40.953740341241016	26.76949267550384	16.596904780546172	15.67986220270897
11	30.940952335065834	26.580382756869287	23.153518307727207	19.325146600337668
12	29.49070811827601	22.180866974622734	28.20829393543362	20.120130971667642
13	25.54690307414032	23.798138210843504	29.09602648341875	21.558932231597417
14	21.369420805704614	25.37927379382201	29.69146174739974	23.55984365307364
15	20.14281613175862	26.08090772760945	32.2434418808206	21.53283425981133
16	23.21976700533804	24.314677298578864	31.086097208922293	21.3794584871608
17	23.077031175031067	24.47809075268558	28.896477376069768	23.548400696213587
18	23.427145504222853	24.63628461243508	29.759717981301808	22.17685190204026
19	25.60431861206971	27.154939643421404	26.351322866039112	20.889418878469776
20	28.043475205923034	25.939376419077213	26.848388851749466	19.16875952325028
21	26.797397429952042	26.75343238517394	26.433230346721594	20.015939838152423
22	24.973349955733827	24.68426472979565	27.057975640554645	23.284409673915878
23	24.343385067543558	25.475234028543152	28.034842799870713	22.146538104042577
24	24.49575707204847	24.690688845927614	28.403426462941884	22.410127619082036
25	23.50122359336951	25.876339779532366	26.50570240683526	24.116734220262867
26	22.513917245339	27.181840629723986	27.495217044786123	22.809025080150885
27	22.238483266181245	26.736970587585795	29.25943993752547	21.76510620870749
28	21.556523188047933	26.97646966713041	29.28192434398733	22.185082800834333
29	22.06463062336009	25.977519608610727	29.202024399596084	22.755825368433097
30	22.904784561242906	25.48466944911197	28.86134549097311	22.749200498672014
31	23.03567592743158	27.065805032090466	27.262342835002602	22.636176205475355
32	24.379319967156707	27.610248874274024	26.52236495805253	21.48806620051674
33	24.668204439465754	26.942743057437617	25.959050274731343	22.430002228365282
34	21.955420649116782	26.382640432182413	28.589725830768703	23.0722130879321
35	22.011832418900553	26.268010109952762	28.949074826900183	22.7710826442465
36	22.58096895746633	28.229573820120734	27.159155469633	22.030301752779934
37	23.2101308311401	28.917757260756883	27.377976925377865	20.49413498272515
38	22.976052099581832	27.255517211612396	26.727936674275227	23.040494014530548
39	22.340265356146976	26.286278690203023	27.151326078097178	24.222129875552824
40	23.173392917010457	25.234329673594676	28.369298345990853	23.22297906340402
41	20.639480610210732	26.468362231818244	28.81999024337362	24.0721669145974
42	22.56531017439468	26.17967851313832	27.300084517277863	23.95492679518914
43	22.79738136966171	25.217265615119157	28.2149188051947	23.770434210024433
44	22.06061555077762	24.852697024630462	28.54596153961973	24.540725884972186
45	22.204555902859333	25.214856571569673	27.071225380076807	25.509362145494187
46	21.516573215852308	26.558701364923927	28.034441292612467	23.8902841266113
47	20.44836315528494	26.342288952728545	29.73362000951572	23.475727882470796
48	21.592056580402833	24.635080090660338	30.558115164326882	23.214748164609947
49	22.269600078695422	24.5662215958709	29.533669394908486	23.630508930525192
50	20.857900558697352	25.16366439614312	28.697129022349905	25.28130602280963
51	20.254234395922293	25.259624630864263	27.82907033001889	26.65707064319455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	296.5
2	504.0
3	818.0
4	1132.0
5	877.5
6	623.0
7	649.0
8	675.0
9	689.0
10	703.0
11	832.5
12	962.0
13	972.5
14	983.0
15	997.0
16	1011.0
17	1118.0
18	1225.0
19	1268.0
20	1311.0
21	1428.5
22	1546.0
23	1671.0
24	1796.0
25	2409.0
26	4131.5
27	5241.0
28	5991.5
29	6742.0
30	7732.5
31	8723.0
32	10219.5
33	11716.0
34	13777.5
35	15839.0
36	16704.0
37	17569.0
38	19115.5
39	20662.0
40	22357.0
41	24052.0
42	27017.5
43	29983.0
44	31264.5
45	32546.0
46	35497.0
47	38448.0
48	40445.0
49	42442.0
50	41738.5
51	41035.0
52	37985.0
53	34935.0
54	31513.0
55	28091.0
56	27320.0
57	26549.0
58	25570.5
59	24592.0
60	23653.5
61	22715.0
62	20324.0
63	17933.0
64	15112.0
65	12291.0
66	10173.0
67	8055.0
68	6329.5
69	4604.0
70	3822.5
71	3041.0
72	2496.0
73	1951.0
74	1645.0
75	1081.0
76	823.0
77	576.5
78	330.0
79	277.5
80	225.0
81	138.5
82	52.0
83	29.0
84	6.0
85	6.0
86	6.0
87	5.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.675085178100716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41555500059084	25.803657082211263
2	9.081900951892921	6.298313781719219
3	3.1163308315523683	3.2417711108165945
4	1.6874027543969932	2.3404333695390998
5	1.131728766919851	1.9621395695726365
6	0.9423376185837804	1.9605382314552702
7	0.7578677539143641	1.8395390244501515
8	0.6794709781679455	1.884857123521674
9	0.6437357630456737	2.0089433174218554
>10	7.402937199484323	47.478769127986006
>50	0.10672205593366961	2.5050190879201386
>100	0.029905631058335987	1.617892604105563
>500	0.003518309536274822	0.7986781637459661
>1k	5.863849227124704E-4	0.25944840553458576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1276	0.25616163076188014	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	906	0.1818827879860998	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	753	0.15116748273016906	Illumina Single End Adapter 1 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	615	0.12346348191109426	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	585	0.11744087303738233	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	558	0.1120205250510416	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.10258510448222628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19412875936264737	0.0
2	0.0	0.0	0.0	0.7827383999534252	0.0
3	0.0	0.0	0.0	1.0320744073250985	0.0
4	0.0	0.0	0.0	1.3952377224099268	0.0
5	0.0	0.0	0.0	1.99328278356952	0.0
6	0.0	0.0	0.0	2.366483780110535	0.0
7	0.0	0.0	0.0	2.6961212391317004	0.0
8	0.0	0.0	0.0	3.2116565587214403	0.0
9	0.0	0.0	0.0	3.4286712318041928	0.0
10	0.0	0.0	0.0	3.94621408768517	0.0
11	0.0	0.0	0.0	5.013621133736045	0.0
12	0.0	0.0	0.0	5.839521563951072	0.0
13	0.0	0.0	0.0	6.136235427795946	0.0
14	0.0	0.0	0.0	6.232195662517089	0.0
15	0.0	0.0	0.0	6.408457348887724	0.0
16	0.0	0.0	0.0	6.842085187794982	0.0
17	0.0	0.0	0.0	7.486504337282157	0.0
18	0.0	0.0	0.0	8.16685838638248	0.0
19	0.0	0.0	0.0	8.651276893458041	0.0
20	0.0	0.0	0.0	9.131078067063758	0.0
21	0.0	0.0	0.0	9.83993913149965	0.0
22	0.0	0.0	0.0	10.611033820963899	0.0
23	0.0	0.0	0.0	11.425491294318872	0.0
24	0.0	0.0	0.0	12.039195138550117	0.0
25	0.0	0.0	0.0	12.59528269122285	0.0
26	0.0	0.0	0.0	13.08712908257599	0.0
27	0.0	0.0	0.0	13.584797329173718	0.0
28	0.0	0.0	0.0	14.126229866920418	0.0
29	0.0	0.0	0.0	14.678503100639801	0.0
30	0.0	0.0	0.0	15.286786596884705	0.0
31	0.0	0.0	0.0	15.873990962071616	0.0
32	0.0	0.0	0.0	16.44132071797528	0.0
33	0.0	0.0	0.0	17.001824850488735	0.0
34	0.0	0.0	0.0	17.572567418087502	0.0
35	0.0	0.0	0.0	18.147525811897864	0.0
36	0.0	0.0	0.0	18.735332437972147	0.0
37	0.0	0.0	0.0	19.33598729631035	0.0
38	0.0	0.0	0.0	19.942263256264017	0.0
39	0.0	0.0	0.0	20.575640956149385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.029432E-4	45.000004	37
CGTATTA	20	7.029432E-4	45.000004	35
CACGAAC	20	7.029432E-4	45.000004	43
TAACGCG	20	7.029432E-4	45.000004	34
ATTCCGG	30	2.1630149E-6	45.000004	2
ACGTATT	30	2.1630149E-6	45.000004	23
GCAATCA	30	2.1630149E-6	45.000004	9
GCTAACG	20	7.029432E-4	45.000004	32
CGATCAT	20	7.029432E-4	45.000004	10
CGCCCTA	20	7.029432E-4	45.000004	39
TTACTAG	25	3.8876122E-5	45.0	26
TCAGACG	85	0.0	42.352943	22
AAACACG	60	3.6379788E-12	41.250004	40
AGACACG	60	3.6379788E-12	41.250004	24
TATGACG	50	1.0786607E-9	40.5	19
TCGTTCA	95	0.0	40.263157	16
CTCGTAA	80	0.0	39.375004	33
TTTCGCG	190	0.0	39.078945	1
TTGGGAC	800	0.0	38.8125	5
TTCGGGC	290	0.0	38.793102	4
>>END_MODULE