FastQCFastQC Report
Sat 14 Jan 2017
SRR2934076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999960
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC119821.1982479299171966No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG111311.1131445257810313No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC87740.877435097403896No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43670.43671746869874795No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT41640.4164166566662667No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA26530.265310612424497No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC24250.2425097003880155TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT22180.22180887235489422No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC21800.21800872034881394No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT19280.19280771230849233No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC12010.12010480419216768TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT11840.11840473618944757TruSeq Adapter, Index 19 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTT11520.11520460818432737No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG10960.10960438417536701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG207.0328446E-445.0000041
ACACTCG207.0328446E-445.00000429
CGATCGA2750.045.041
CCGATCG2800.044.19642640
GATCGAA2950.041.94915442
CGTTTTT25850.041.866541
CACCCGT700.041.78571338
GATGAAT32900.041.71732720
CCGATGA30500.041.4590218
CTACGGT603.6379788E-1241.25000445
CGATGAA31000.041.22580719
ATGAATG33250.041.0075221
TACGGCT14750.040.8813557
CGAATAG950.040.2631626
TGATACC12650.039.84194
TTGGGAC17550.039.7435875
TTTGGGC20250.039.5555574
TGAATGA34400.039.30959322
ACGGCTG15350.039.2833868
TGGGCGA12850.038.69656
Produced by FastQC (version 0.11.5)