##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999960
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.877204088163527	31.0	30.0	31.0	25.0	34.0
2	29.658233329333175	31.0	30.0	33.0	25.0	34.0
3	29.744763790551623	31.0	30.0	33.0	25.0	34.0
4	33.821563862554505	35.0	33.0	37.0	28.0	37.0
5	34.41716368654746	35.0	35.0	37.0	32.0	37.0
6	33.625117004680185	35.0	33.0	37.0	28.0	37.0
7	34.354207168286734	35.0	35.0	37.0	32.0	37.0
8	34.0022510900436	35.0	35.0	37.0	28.0	37.0
9	35.75448117924717	37.0	35.0	39.0	31.0	39.0
10	35.32299291971679	37.0	34.0	39.0	30.0	39.0
11	35.45408916356654	37.0	35.0	39.0	30.0	39.0
12	35.45691327653106	37.0	35.0	39.0	30.0	39.0
13	35.38972558902356	37.0	35.0	39.0	30.0	39.0
14	36.10247109884396	38.0	35.0	40.0	29.0	41.0
15	36.27359394375775	38.0	35.0	40.0	30.0	41.0
16	36.557604304172166	38.0	35.0	40.0	31.0	41.0
17	36.144632785311416	38.0	34.0	40.0	30.0	41.0
18	36.32479299171967	38.0	35.0	40.0	30.0	41.0
19	36.259434377375094	38.0	35.0	40.0	30.0	41.0
20	36.05170306812273	38.0	34.0	40.0	29.0	41.0
21	35.93249229969199	38.0	34.0	40.0	29.0	41.0
22	36.125508020320815	38.0	34.0	40.0	30.0	41.0
23	36.00592723708948	38.0	34.0	40.0	30.0	41.0
24	35.989487579503184	38.0	34.0	40.0	30.0	41.0
25	35.67513800552022	38.0	34.0	40.0	29.0	41.0
26	35.716611664466576	38.0	34.0	40.0	29.0	41.0
27	35.59921296851874	38.0	34.0	40.0	28.0	41.0
28	35.48782951318053	38.0	34.0	40.0	27.0	41.0
29	35.630759230369215	38.0	34.0	40.0	29.0	41.0
30	35.18978659146366	37.0	34.0	40.0	27.0	41.0
31	35.40970438817553	38.0	34.0	40.0	27.0	41.0
32	35.19286471458858	38.0	34.0	40.0	26.0	41.0
33	35.11713168526741	38.0	34.0	40.0	26.0	41.0
34	34.989589583583346	38.0	34.0	40.0	25.0	41.0
35	34.97559702388096	38.0	34.0	40.0	25.0	41.0
36	34.66222148885956	38.0	33.0	40.0	24.0	41.0
37	34.68989059562382	38.0	33.0	40.0	24.0	41.0
38	34.76791871674867	37.0	33.0	40.0	24.0	41.0
39	34.625595023800955	37.0	33.0	40.0	24.0	41.0
40	34.60067702708108	37.0	33.0	40.0	24.0	41.0
41	34.54072762910516	37.0	33.0	40.0	24.0	41.0
42	34.497627905116204	37.0	33.0	40.0	24.0	41.0
43	34.43910056402256	37.0	33.0	40.0	24.0	41.0
44	34.55965638625545	37.0	33.0	40.0	24.0	41.0
45	34.5657536301452	37.0	33.0	40.0	24.0	41.0
46	34.51824472978919	37.0	33.0	40.0	24.0	41.0
47	34.34271670866835	37.0	33.0	40.0	24.0	41.0
48	34.31905776231049	37.0	33.0	40.0	24.0	41.0
49	34.36833273330933	37.0	33.0	40.0	24.0	41.0
50	34.1602504100164	37.0	33.0	40.0	24.0	41.0
51	33.17690707628305	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	10.0
11	20.0
12	20.0
13	27.0
14	31.0
15	63.0
16	124.0
17	289.0
18	609.0
19	1345.0
20	3046.0
21	5525.0
22	8338.0
23	10751.0
24	12630.0
25	13964.0
26	15203.0
27	16749.0
28	19695.0
29	24659.0
30	31262.0
31	40324.0
32	50823.0
33	65809.0
34	86984.0
35	90744.0
36	109615.0
37	137556.0
38	166636.0
39	87099.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.628985159406373	4.001260050402016	49.3113724548982	17.058382335293413
2	20.518120724828993	12.519800792031683	47.709808392335695	19.252270090803634
3	19.951798071922877	12.48709948397936	48.1976279051162	19.36347453898156
4	18.098623944957797	4.569182767310692	52.576303052122086	24.755890235609424
5	24.347373894955798	5.103204128165126	46.735469418776745	23.813952558102326
6	20.374314972598903	13.378835153406134	49.55768230729229	16.689167566702668
7	77.18188727549102	2.048781951278051	16.89157566302652	3.8777551102044083
8	78.4549381975279	9.413476539061563	7.8659146365854635	4.265670626825073
9	73.48223928957158	4.429977199087964	9.244669786791473	12.843113724548981
10	43.25963038521541	24.176267050682025	17.216188647545902	15.347913916556664
11	33.50344013760551	22.64770590823633	25.6551262050482	18.193727749109964
12	26.300252010080406	19.954698187927516	29.096063842553704	24.648985959438377
13	27.62900516020641	19.948597943917758	33.10672426897076	19.315672626905076
14	20.910236409456378	27.172086883475338	30.77393095723829	21.143745749829993
15	18.669246769870796	22.739009560382414	35.55942237689508	23.032321292851716
16	22.710508420336815	22.459098363934558	29.485779431177246	25.344613784551385
17	22.5180007200288	26.29395175807032	30.62622504900196	20.561822472898918
18	22.33239329573183	22.402496099843994	30.015600624024962	25.249509980399214
19	22.34989399575983	24.99769990799632	28.60594423776951	24.046461858474338
20	27.268890755630228	24.85259410376415	30.61532461298452	17.263190527621106
21	24.711788471538863	28.78405136205448	28.49163966558662	18.012520500820035
22	22.680107204288174	21.53746149845994	33.90505620224809	21.8773750950038
23	25.213708548341934	25.408016320652827	29.255270210808433	20.123004920196806
24	22.399295971838875	26.65316612664507	28.481139245569825	22.466398655946236
25	21.931077243089725	29.595383815352616	26.431757270290813	22.04178167126685
26	20.299311972478897	24.84689387575503	31.37565502620105	23.478139125565022
27	24.004560182407296	25.34851394055762	28.477639105564222	22.16928677147086
28	19.046161846473858	28.322332893315732	30.072002880115207	22.559502380095203
29	22.306492259690387	24.08626345053802	31.477659106364253	22.129585183407336
30	23.049221968878754	27.618104724188967	28.87545501820073	20.45721828873155
31	23.055322212888516	23.657646305852236	26.75147005880235	26.5355614224569
32	26.392155686227447	26.720868834753393	26.60626425057002	20.28071122844914
33	26.61036441457658	23.4339373574943	26.308852354094164	23.646845873834955
34	22.15778631145246	23.764850594023763	29.30947237889516	24.767890715628624
35	24.287371494859794	24.926997079883193	28.772450898035924	22.01318052722109
36	21.58296331853274	27.240289611584462	28.922056882275292	22.254690187607505
37	22.37579503180127	26.70296811872475	27.838713548541943	23.08252330093204
38	22.46629865194608	25.25861034441378	26.312852514100566	25.96223848953958
39	21.655366214648588	27.849213968558743	26.952978119124765	23.542441697667908
40	22.7878115124605	22.749809992399697	29.464578583143325	24.99779991199648
41	19.558382335293413	23.842453698147924	28.966658666346657	27.632505300212006
42	21.434757390295612	23.77095083803352	31.61796471858874	23.176327053082126
43	20.703028121124845	24.69408776351054	30.50182007280291	24.101064042561703
44	23.083723348933958	23.16082643305732	30.00190007600304	23.75355014200568
45	21.68716748669947	22.80321212848514	27.95641825673027	27.553202128085125
46	23.349533981359254	26.491059642385693	27.88121524860994	22.278191127645105
47	19.571782871314852	26.100544021760868	32.402596103844154	21.925077003080123
48	21.10184407376295	25.526521060842434	29.125265010600426	24.246369854794192
49	22.162386495459817	22.85501420056802	31.96287851514061	23.019720788831552
50	21.666466658666348	23.94825793031721	29.529581183247327	24.85569422776911
51	20.536721468858754	23.474838993559743	29.52428097123885	26.464158566342654
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	523.0
2	841.0
3	4066.0
4	7291.0
5	5114.5
6	2938.0
7	2803.5
8	2669.0
9	2785.0
10	2901.0
11	2901.5
12	2902.0
13	2896.5
14	2891.0
15	2805.5
16	2720.0
17	2847.0
18	2974.0
19	2969.0
20	2964.0
21	3184.0
22	3404.0
23	4109.5
24	4815.0
25	5757.5
26	8619.0
27	10538.0
28	11831.0
29	13124.0
30	15251.0
31	17378.0
32	19629.0
33	21880.0
34	24449.0
35	27018.0
36	29464.0
37	31910.0
38	33838.5
39	35767.0
40	38889.5
41	42012.0
42	45528.5
43	49045.0
44	51886.5
45	54728.0
46	82175.0
47	109622.0
48	99030.0
49	88438.0
50	86137.0
51	83836.0
52	78623.5
53	73411.0
54	68493.0
55	63575.0
56	59276.5
57	54978.0
58	51515.0
59	48052.0
60	43324.5
61	38597.0
62	34686.5
63	30776.0
64	26776.5
65	22777.0
66	18548.5
67	14320.0
68	11450.0
69	8580.0
70	7121.5
71	5663.0
72	4556.5
73	3450.0
74	2865.0
75	1603.5
76	927.0
77	751.5
78	576.0
79	456.0
80	336.0
81	214.0
82	92.0
83	71.0
84	50.0
85	28.0
86	6.0
87	4.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	999960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.903638556943125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.22029742712508	23.678975427038136
2	8.056010226857548	5.1403207697740125
3	2.9460667069116884	2.819707421458626
4	1.633134094602058	2.084116794768185
5	1.25080562585717	1.9952625296169095
6	1.0710402137395028	2.050204791545773
7	0.9266262711197774	2.0693924741821355
8	0.8611361708878036	2.19787017154516
9	0.8126484425714311	2.333379796709552
>10	8.08894710801843	44.942331999703946
>50	0.08684385910932028	1.8833204882512486
>100	0.03880924719483634	2.316366967085096
>500	0.003181085835604476	0.7592333193413874
>1k	0.0034991944191649234	2.4933624100493526
>5k	3.181085835604476E-4	0.8904575783854208
>10k+	6.362171671208951E-4	2.345697060545046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	11982	1.1982479299171966	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	11131	1.1131445257810313	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	8774	0.877435097403896	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4367	0.43671746869874795	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT	4164	0.4164166566662667	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA	2653	0.265310612424497	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2425	0.2425097003880155	TruSeq Adapter, Index 13 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT	2218	0.22180887235489422	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC	2180	0.21800872034881394	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT	1928	0.19280771230849233	No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	1201	0.12010480419216768	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	1184	0.11840473618944757	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTT	1152	0.11520460818432737	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1096	0.10960438417536701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12620504820192807	0.0
2	0.0	0.0	0.0	0.6158246329853194	0.0
3	0.0	0.0	0.0	0.8199327973118925	0.0
4	0.0	0.0	0.0	1.3194527781111245	0.0
5	0.0	0.0	0.0	2.7405096203848154	0.0
6	0.0	0.0	0.0	3.28663146525861	0.0
7	0.0	0.0	0.0	3.844453778151126	0.0
8	0.0	0.0	0.0	4.551282051282051	0.0
9	0.0	0.0	0.0	4.813592543701748	0.0
10	0.0	0.0	0.0	6.493659746389856	0.0
11	0.0	0.0	0.0	7.295291811672467	0.0
12	0.0	0.0	0.0	9.37457498299932	0.0
13	0.0	0.0	0.0	9.68178727149086	0.0
14	0.0	0.0	0.0	9.85319412776511	0.0
15	0.0	0.0	0.0	10.294311772470898	0.0
16	0.0	0.0	0.0	10.650726029041161	0.0
17	0.0	0.0	0.0	11.058642345693828	0.0
18	0.0	0.0	0.0	11.461558462338493	0.0
19	0.0	0.0	0.0	12.33359334373375	0.0
20	0.0	0.0	0.0	12.723808952358095	0.0
21	0.0	0.0	0.0	13.193627745109804	0.0
22	0.0	0.0	0.0	13.723448937957519	0.0
23	0.0	0.0	0.0	14.22366894675787	0.0
24	0.0	0.0	0.0	14.639685587423497	0.0
25	0.0	0.0	0.0	15.00320012800512	0.0
26	0.0	0.0	0.0	15.352614104564182	0.0
27	0.0	0.0	0.0	15.707028281131246	0.0
28	0.0	0.0	0.0	16.07334293371735	0.0
29	0.0	0.0	0.0	16.465458618344734	0.0
30	0.0	0.0	0.0	16.930677227089085	0.0
31	0.0	0.0	0.0	17.34629385175407	0.0
32	0.0	0.0	0.0	17.756010240409616	0.0
33	0.0	0.0	0.0	18.160426417056684	0.0
34	0.0	0.0	0.0	18.58994359774391	0.0
35	0.0	0.0	0.0	19.04146165846634	0.0
36	0.0	0.0	0.0	19.463578543141725	0.0
37	0.0	0.0	0.0	19.904996199847993	0.0
38	0.0	0.0	0.0	20.405716228649148	0.0
39	0.0	0.0	0.0	21.088643545741828	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	20	7.0328446E-4	45.000004	1
ACACTCG	20	7.0328446E-4	45.000004	29
CGATCGA	275	0.0	45.0	41
CCGATCG	280	0.0	44.196426	40
GATCGAA	295	0.0	41.949154	42
CGTTTTT	2585	0.0	41.86654	1
CACCCGT	70	0.0	41.785713	38
GATGAAT	3290	0.0	41.717327	20
CCGATGA	3050	0.0	41.45902	18
CTACGGT	60	3.6379788E-12	41.250004	45
CGATGAA	3100	0.0	41.225807	19
ATGAATG	3325	0.0	41.00752	21
TACGGCT	1475	0.0	40.881355	7
CGAATAG	95	0.0	40.26316	26
TGATACC	1265	0.0	39.8419	4
TTGGGAC	1755	0.0	39.743587	5
TTTGGGC	2025	0.0	39.555557	4
TGAATGA	3440	0.0	39.309593	22
ACGGCTG	1535	0.0	39.283386	8
TGGGCGA	1285	0.0	38.6965	6
>>END_MODULE