SRR2934073.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	3565	0.6914998855582237	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	2989	0.5797736768397	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	2525	0.4897720087053338	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2019	0.3916236378519085	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	1351	0.2620522707963984	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1304	0.25293572251554663	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	1253	0.24304329778526068	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	901	0.17476617023505178	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	882	0.17108075710023934	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	869	0.16855915863957824	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	827	0.1604124559205192	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	764	0.1481924018419307	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	657	0.12743770681956604	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	614	0.11909703498814848	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.11036842493201383	No Hit
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	565	0.10959254848257963	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	565	0.10959254848257963	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	547	0.10610110446012577	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	536	0.10396744422418173	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	90	0.0	45.000004	40
CGATCGA	90	0.0	45.000004	41
TCCGGCA	20	7.0296624E-4	45.0	15
TCGTTGA	35	1.2101918E-7	45.0	24
AACCCGC	20	7.0296624E-4	45.0	39
GGGCGTA	20	7.0296624E-4	45.0	7
ACTACCG	20	7.0296624E-4	45.0	16
TACGTTG	20	7.0296624E-4	45.0	24
TACGGGA	50	1.0786607E-9	40.5	4
TACGGCT	435	0.0	40.34483	7
GCGATTC	145	0.0	40.34483	9
CGATGAA	635	0.0	40.03937	19
CCGGGTA	45	1.9246727E-8	40.000004	5
TACGAAT	90	0.0	40.000004	12
TTGACAC	90	0.0	40.000004	28
CGGGATA	90	0.0	40.000004	6
CCGATGA	630	0.0	39.999996	18
GCGATCT	170	0.0	39.705883	9
CCTATGC	85	0.0	39.705883	35
TGGGCGA	800	0.0	39.65625	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content