##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.949655316887338	31.0	30.0	31.0	26.0	34.0
2	30.048030631602224	31.0	30.0	33.0	25.0	34.0
3	30.080636839389694	31.0	30.0	34.0	25.0	34.0
4	33.907121769929354	35.0	33.0	37.0	28.0	37.0
5	34.391790451288536	35.0	35.0	37.0	32.0	37.0
6	33.81465475437691	35.0	35.0	37.0	29.0	37.0
7	34.36529427054036	35.0	35.0	37.0	32.0	37.0
8	34.33452882962917	35.0	35.0	37.0	31.0	37.0
9	35.85708549770535	37.0	35.0	39.0	31.0	39.0
10	35.307887172046726	37.0	34.0	39.0	30.0	39.0
11	35.416833803385146	37.0	35.0	39.0	30.0	39.0
12	35.386623114135304	37.0	35.0	39.0	30.0	39.0
13	35.30042518029429	37.0	34.0	39.0	30.0	39.0
14	35.861443983660045	38.0	34.0	40.0	27.0	41.0
15	36.101465242674756	38.0	34.0	40.0	29.0	41.0
16	36.35523503237344	38.0	35.0	40.0	30.0	41.0
17	36.12474735523115	38.0	34.0	40.0	30.0	41.0
18	36.3052045792228	38.0	35.0	40.0	30.0	41.0
19	36.25568426483767	38.0	35.0	40.0	30.0	41.0
20	36.19923149437683	38.0	34.0	40.0	30.0	41.0
21	35.951519360057105	38.0	34.0	40.0	29.0	41.0
22	36.17817420753919	38.0	34.0	40.0	30.0	41.0
23	36.21876418399134	38.0	34.0	40.0	30.0	41.0
24	36.146791944850705	38.0	34.0	40.0	30.0	41.0
25	35.72778956678938	38.0	34.0	40.0	29.0	41.0
26	35.85443200024828	38.0	34.0	40.0	30.0	41.0
27	35.829280025448746	38.0	34.0	40.0	29.0	41.0
28	35.74568127771334	38.0	34.0	40.0	29.0	41.0
29	35.85799909222455	38.0	34.0	40.0	29.0	41.0
30	35.44901133943431	38.0	34.0	40.0	28.0	41.0
31	35.56185093085777	38.0	34.0	40.0	29.0	41.0
32	35.51453410558903	38.0	34.0	40.0	28.0	41.0
33	35.555048434087354	38.0	34.0	40.0	29.0	41.0
34	35.48499260977682	38.0	34.0	40.0	28.0	41.0
35	35.48571805425704	38.0	34.0	40.0	28.0	41.0
36	35.27078282054366	38.0	34.0	40.0	27.0	41.0
37	35.353353531983565	38.0	34.0	40.0	28.0	41.0
38	35.20807066682701	38.0	34.0	40.0	27.0	41.0
39	35.160938112215014	37.0	34.0	40.0	27.0	41.0
40	35.07578955127185	37.0	33.0	40.0	27.0	41.0
41	34.881413103777355	37.0	33.0	40.0	26.0	41.0
42	35.12636117824598	37.0	33.0	40.0	27.0	41.0
43	35.10517393210305	37.0	34.0	40.0	27.0	41.0
44	35.11946751599275	37.0	34.0	40.0	27.0	41.0
45	35.15946006757884	37.0	34.0	40.0	28.0	41.0
46	35.07694948656376	37.0	34.0	40.0	27.0	41.0
47	34.967446163872864	37.0	33.0	40.0	27.0	41.0
48	34.94614253626253	37.0	33.0	40.0	27.0	41.0
49	34.975707308368214	37.0	34.0	40.0	27.0	41.0
50	34.76927955992288	36.0	33.0	40.0	27.0	41.0
51	33.70279858635311	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	8.0
11	7.0
12	12.0
13	15.0
14	19.0
15	36.0
16	81.0
17	139.0
18	253.0
19	459.0
20	939.0
21	1578.0
22	2415.0
23	3241.0
24	4297.0
25	5318.0
26	6474.0
27	7824.0
28	9986.0
29	12728.0
30	16686.0
31	21042.0
32	27391.0
33	35573.0
34	47718.0
35	48619.0
36	59028.0
37	74671.0
38	86549.0
39	42436.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.466891412211517	4.724505669717154	52.453321333110914	16.35528158496041
2	20.360743755164428	8.176379993249874	50.735336904951254	20.72753934663444
3	21.228367594744213	8.116249568418725	49.723594014889066	20.931788821947993
4	19.759827445077647	4.929143083255423	49.3556346087449	25.955394862922027
5	21.37811174948501	5.509498667432198	47.70204792588828	25.410341657194508
6	21.834909009089394	9.47830067540045	50.50238000100864	18.18441031450152
7	81.75390750776846	2.226765409876131	11.883129730421729	4.136197351933678
8	82.93071811244778	5.769417277992652	6.296625325383186	5.003239284176388
9	78.16819449670834	4.817416874536899	8.291597645990853	8.722790982763904
10	41.94116528883941	27.72225950739604	15.75145573818825	14.585119465576302
11	32.87931629767276	24.1053174692462	24.074670349493545	18.940695883587498
12	27.182831405926922	21.286558328451775	28.678527231323685	22.852083034297618
13	26.266715288257497	23.479185174552804	30.588735049830667	19.66536448735903
14	21.0192688916217	26.971017135231385	30.282651790528874	21.72706218261804
15	18.287795851388626	24.075252256830623	34.450854046001716	23.186097845779038
16	22.867794532398662	23.580631020316325	29.266253641770085	24.285320805514928
17	22.847427775601016	25.478618784744718	28.258002195730352	23.415951243923917
18	24.312864419469843	22.29597358916566	30.16646429222611	23.22469769913839
19	23.86576561548339	24.927358567421724	26.939400169916944	24.267475647177942
20	25.851815356922565	26.71614172159225	28.821482467131933	18.610560454353248
21	24.612934636288518	28.286515655247058	28.022135755102358	19.078413953362066
22	23.09648411586939	22.853828756308843	31.00208322826673	23.047603899555035
23	23.035577814588805	25.82679334142831	28.988295903760285	22.1493329402226
24	22.652100879455954	26.347212469886294	27.912543206619777	23.08814344403797
25	22.044201681324267	28.931462953839233	25.66036784302468	23.363967521811826
26	20.964569601936585	25.612457472272116	27.84290829528306	25.580064630508236
27	22.048856940020872	25.448553572329143	28.904501247221393	23.598088240428595
28	17.936517788907295	27.034832973197346	30.42366733521354	24.604981902681818
29	20.80376920779135	25.620604174991175	29.58979412118414	23.985832496033332
30	22.91706268693773	25.79226683942849	29.29088771903962	21.999782754594158
31	25.087771023342242	25.252838737959365	26.929507745186655	22.72988249351173
32	26.25333141950476	26.943279552164114	24.879642165781522	21.923746862549606
33	24.9279404747588	25.496076004856988	24.90117273725332	24.674810783130894
34	22.36308690204172	24.56192075973822	29.697834916767853	23.377157421452207
35	22.803784725320337	25.948024036652406	26.91980928956873	24.328381948458528
36	23.443494857878832	27.78568740713729	26.29309508753826	22.47772264744562
37	20.819286736780036	27.902262843664776	27.114166340151996	24.164284079403195
38	21.930341812369797	28.41725083697672	26.482214972087846	23.170192378565638
39	23.123639791599583	28.059183855562843	25.836879735270955	22.980296617566616
40	26.799160501681712	23.421964286407032	26.702757852839515	23.07611735907174
41	22.38403556617644	24.357865253537025	26.498314408413602	26.759784771872923
42	22.692252485714175	24.360774790222408	28.365655053089345	24.581317670974073
43	21.07086467550907	26.058198492472055	28.167612589371267	24.7033242426476
44	22.346793496603603	24.017643430460133	28.79413282228938	24.841430250646887
45	22.700399188433234	23.487331877271863	26.263999720684478	27.54826921361043
46	23.162239644958937	25.308119934981555	27.440810325363792	24.08883009469572
47	19.147466957361708	26.38270881744791	31.445302650006013	23.024521575184366
48	20.9841604822848	27.12929593091596	28.42753119993172	23.459012386867514
49	23.656472943248517	24.029087608089288	29.491839719443075	22.82259972921912
50	22.235649195222155	25.29958529403778	27.325980610847527	25.138784899892542
51	21.28112719330574	24.065941739437413	26.813708185108602	27.839222882148245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	120.0
1	267.5
2	415.0
3	736.5
4	1058.0
5	785.0
6	512.0
7	584.0
8	656.0
9	739.5
10	823.0
11	878.5
12	934.0
13	980.0
14	1026.0
15	1051.0
16	1076.0
17	1060.5
18	1045.0
19	1074.0
20	1103.0
21	1269.5
22	1436.0
23	1556.0
24	1676.0
25	2298.0
26	3515.0
27	4110.0
28	4768.0
29	5426.0
30	6331.0
31	7236.0
32	9391.5
33	11547.0
34	12781.5
35	14016.0
36	14970.5
37	15925.0
38	17010.5
39	18096.0
40	20737.5
41	23379.0
42	25314.0
43	27249.0
44	30399.5
45	33550.0
46	42733.0
47	51916.0
48	51649.0
49	51382.0
50	49988.0
51	48594.0
52	43593.5
53	38593.0
54	36363.0
55	34133.0
56	31672.0
57	29211.0
58	26787.0
59	24363.0
60	20572.5
61	16782.0
62	14604.5
63	12427.0
64	11859.5
65	11292.0
66	9665.0
67	8038.0
68	6549.0
69	5060.0
70	4357.0
71	3654.0
72	2870.0
73	2086.0
74	1743.0
75	1025.5
76	651.0
77	506.0
78	361.0
79	260.5
80	160.0
81	112.0
82	64.0
83	49.5
84	35.0
85	21.0
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	515546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.93548605060111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.08836034669034	25.14224519057018
2	9.578226521103355	6.500835449928009
3	3.375089717725403	3.4360593010619285
4	1.790054616973304	2.429854939364466
5	1.1854278667461033	2.0114035417978107
6	0.9039919801055014	1.8406444338435308
7	0.7570050184630193	1.7982533271000771
8	0.6536218138900527	1.7744779158107578
9	0.6530009827233236	1.9943915166212542
>10	6.846966756159471	43.12600086312556
>50	0.10870042381779497	2.5191768741393314
>100	0.04741188698435736	2.6549561713512957
>500	0.008094712411963452	1.8273303395014866
>1k	0.004047356205981726	2.9443701357843133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	3565	0.6914998855582237	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	2989	0.5797736768397	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	2525	0.4897720087053338	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2019	0.3916236378519085	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	1351	0.2620522707963984	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	1304	0.25293572251554663	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	1253	0.24304329778526068	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	901	0.17476617023505178	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	882	0.17108075710023934	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	869	0.16855915863957824	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	827	0.1604124559205192	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	764	0.1481924018419307	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	657	0.12743770681956604	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	614	0.11909703498814848	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.11036842493201383	No Hit
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	565	0.10959254848257963	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	565	0.10959254848257963	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	547	0.10610110446012577	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	536	0.10396744422418173	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28009139824574336	0.0
2	0.0	0.0	0.0	1.1898065352073335	0.0
3	0.0	0.0	0.0	1.5160625821944114	0.0
4	0.0	0.0	0.0	2.157712405876488	0.0
5	0.0	0.0	0.0	3.4458612810496057	0.0
6	0.0	0.0	0.0	3.9492111276200377	0.0
7	0.0	0.0	0.0	4.554588727291066	0.0
8	0.0	0.0	0.0	5.484088713713228	0.0
9	0.0	0.0	0.0	5.793081509700396	0.0
10	0.0	0.0	0.0	7.047479759323125	0.0
11	0.0	0.0	0.0	8.391103800630788	0.0
12	0.0	0.0	0.0	10.393059009283363	0.0
13	0.0	0.0	0.0	10.838800029483306	0.0
14	0.0	0.0	0.0	11.009298879246469	0.0
15	0.0	0.0	0.0	11.383465296986108	0.0
16	0.0	0.0	0.0	11.88409957598352	0.0
17	0.0	0.0	0.0	12.588983330294484	0.0
18	0.0	0.0	0.0	13.366411532627545	0.0
19	0.0	0.0	0.0	14.175650669387407	0.0
20	0.0	0.0	0.0	14.804692500766178	0.0
21	0.0	0.0	0.0	15.59918998498679	0.0
22	0.0	0.0	0.0	16.454981708712705	0.0
23	0.0	0.0	0.0	17.226978775899727	0.0
24	0.0	0.0	0.0	17.853499008817835	0.0
25	0.0	0.0	0.0	18.42241041536546	0.0
26	0.0	0.0	0.0	18.933325057317873	0.0
27	0.0	0.0	0.0	19.482451614404923	0.0
28	0.0	0.0	0.0	20.010241569132532	0.0
29	0.0	0.0	0.0	20.621632211286673	0.0
30	0.0	0.0	0.0	21.405267425215207	0.0
31	0.0	0.0	0.0	21.977476306672926	0.0
32	0.0	0.0	0.0	22.517098377254406	0.0
33	0.0	0.0	0.0	23.08445803090316	0.0
34	0.0	0.0	0.0	23.692745167259567	0.0
35	0.0	0.0	0.0	24.37881391767175	0.0
36	0.0	0.0	0.0	24.94908310800588	0.0
37	0.0	0.0	0.0	25.543210499160114	0.0
38	0.0	0.0	0.0	26.20367532674097	0.0
39	0.0	0.0	0.0	27.24431961454458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	90	0.0	45.000004	40
CGATCGA	90	0.0	45.000004	41
TCCGGCA	20	7.0296624E-4	45.0	15
TCGTTGA	35	1.2101918E-7	45.0	24
AACCCGC	20	7.0296624E-4	45.0	39
GGGCGTA	20	7.0296624E-4	45.0	7
ACTACCG	20	7.0296624E-4	45.0	16
TACGTTG	20	7.0296624E-4	45.0	24
TACGGGA	50	1.0786607E-9	40.5	4
TACGGCT	435	0.0	40.34483	7
GCGATTC	145	0.0	40.34483	9
CGATGAA	635	0.0	40.03937	19
CCGGGTA	45	1.9246727E-8	40.000004	5
TACGAAT	90	0.0	40.000004	12
TTGACAC	90	0.0	40.000004	28
CGGGATA	90	0.0	40.000004	6
CCGATGA	630	0.0	39.999996	18
GCGATCT	170	0.0	39.705883	9
CCTATGC	85	0.0	39.705883	35
TGGGCGA	800	0.0	39.65625	6
>>END_MODULE