##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	158638
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.019680026223224	31.0	30.0	31.0	26.0	34.0
2	30.400288707623645	31.0	30.0	33.0	27.0	34.0
3	30.36014069768908	31.0	30.0	34.0	26.0	34.0
4	34.0087999092273	35.0	33.0	37.0	28.0	37.0
5	34.38826762818493	35.0	35.0	37.0	32.0	37.0
6	33.93852040494711	35.0	35.0	37.0	30.0	37.0
7	34.4622410771694	35.0	35.0	37.0	32.0	37.0
8	34.677151754308554	35.0	35.0	37.0	32.0	37.0
9	35.92775375382947	37.0	35.0	39.0	31.0	39.0
10	35.258891312295916	37.0	34.0	39.0	30.0	39.0
11	35.36167248704598	37.0	34.0	39.0	30.0	39.0
12	35.21512500157591	37.0	34.0	39.0	30.0	39.0
13	35.08835209722765	37.0	34.0	39.0	29.0	39.0
14	35.66709741676017	38.0	34.0	40.0	27.0	41.0
15	36.06223603424148	38.0	34.0	40.0	30.0	41.0
16	36.26111020058246	38.0	34.0	40.0	30.0	41.0
17	36.07397344898448	38.0	34.0	40.0	30.0	41.0
18	36.224933496388005	38.0	35.0	40.0	30.0	41.0
19	36.20308501115748	38.0	34.0	40.0	30.0	41.0
20	36.275596011044016	38.0	34.0	40.0	30.0	41.0
21	35.921922868417404	38.0	34.0	40.0	29.0	41.0
22	36.09319961169455	38.0	34.0	40.0	30.0	41.0
23	36.20754169871027	38.0	34.0	40.0	30.0	41.0
24	36.08107137003744	38.0	34.0	40.0	30.0	41.0
25	35.58349197544094	38.0	34.0	40.0	29.0	41.0
26	35.69964321284938	38.0	34.0	40.0	29.0	41.0
27	35.7644826586316	38.0	34.0	40.0	29.0	41.0
28	35.68657572586644	38.0	34.0	40.0	29.0	41.0
29	35.83879650525095	38.0	34.0	40.0	30.0	41.0
30	35.38790831957034	37.0	34.0	40.0	28.0	41.0
31	35.53081859327526	37.0	34.0	40.0	29.0	41.0
32	35.5904575196359	38.0	34.0	40.0	29.0	41.0
33	35.57777455590716	38.0	34.0	40.0	29.0	41.0
34	35.56921418575625	38.0	34.0	40.0	29.0	41.0
35	35.57775564492744	38.0	34.0	40.0	29.0	41.0
36	35.35076715541043	37.0	34.0	40.0	28.0	41.0
37	35.38117601079187	37.0	34.0	40.0	29.0	41.0
38	35.310694789394724	37.0	34.0	40.0	28.0	41.0
39	35.31252285076715	37.0	34.0	40.0	28.0	41.0
40	35.226629180902435	37.0	34.0	40.0	28.0	41.0
41	35.11388191984266	37.0	34.0	40.0	27.0	41.0
42	35.34465890896254	37.0	34.0	40.0	28.0	41.0
43	35.258973259874686	37.0	34.0	40.0	28.0	41.0
44	35.139386527818054	37.0	34.0	40.0	27.0	41.0
45	35.25805292552856	37.0	34.0	40.0	28.0	41.0
46	35.14898700185328	37.0	34.0	40.0	28.0	41.0
47	35.10451468122392	37.0	33.0	40.0	28.0	41.0
48	35.11572889219481	37.0	34.0	40.0	28.0	41.0
49	35.120954626256	37.0	34.0	40.0	28.0	41.0
50	34.933578335581636	36.0	33.0	40.0	27.0	41.0
51	33.798093773244744	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	6.0
12	1.0
13	1.0
14	3.0
15	6.0
16	21.0
17	40.0
18	66.0
19	130.0
20	210.0
21	343.0
22	514.0
23	753.0
24	1027.0
25	1446.0
26	1917.0
27	2379.0
28	3058.0
29	3939.0
30	5165.0
31	6561.0
32	8479.0
33	11413.0
34	16212.0
35	15933.0
36	17910.0
37	22943.0
38	26585.0
39	11576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.89237131078304	4.39932424765819	56.61632143622587	16.091983005332896
2	19.94225847527074	3.639733229112823	55.184760271813815	21.23324802380262
3	22.16492895775287	3.8679257176716804	53.768958257164115	20.19818706741134
4	20.515891526620354	4.333135818656312	47.754636341860085	27.39633631286325
5	17.75425812226579	5.798106380564556	51.68055573065722	24.76707976651244
6	22.22292262887833	6.373000163895158	53.564089310253536	17.839987896972982
7	84.08262837403396	2.263644271864244	8.925352059405691	4.728375294696101
8	85.34588181898411	2.540374941691146	6.685031329189728	5.428711910135024
9	79.0409611820623	5.54532961837643	9.517265724479634	5.896443475081632
10	41.76615943216632	25.918758431145122	17.77506019995209	14.54002193673647
11	31.778010312787604	24.965014687527578	24.49539202460949	18.761582975075328
12	28.112432078064526	22.580970511478967	29.72427791575789	19.582319494698623
13	22.954777543841956	28.698042083233528	29.560382758229427	18.786797614695093
14	18.64118307089096	30.308627188945902	30.613724328345036	20.4364654118181
15	16.92973940669953	27.006770130737905	37.01446059582194	19.04902986674063
16	19.272179427375534	24.908281748383114	34.72749278230941	21.092046041931944
17	18.58760196169896	24.70530389944402	29.20233487562879	27.50475926322823
18	21.298806086814004	24.305021495480275	31.756577869110806	22.639594548594914
19	22.849506423429446	26.382077434158273	29.034027156166868	21.734388986245413
20	25.698130334472197	27.691347596414477	27.79346688687452	18.817055182238807
21	23.735170640073626	27.233701887315775	28.91930054589695	20.11182692671365
22	21.941149031127473	25.643918859289705	27.610030383640744	24.804901725942084
23	21.476569296133334	26.271763385821806	27.77140407720723	24.48026324083763
24	22.173123715629295	25.102434473455286	30.597965178582687	22.126476632332732
25	19.705240862844967	26.842874973209447	29.127321322759997	24.324562841185593
26	19.071092676407922	29.749492555377653	27.526822072895524	23.6525926953189
27	19.221119782145514	30.048286034871847	30.34392768441357	20.38666649856907
28	17.515979777859027	27.508541459171195	33.22154843101905	21.75393033195073
29	18.23837920296524	26.317780103127873	32.48780241808394	22.956038275822944
30	19.770798925856354	28.95334030938363	29.57677227398227	21.699088490777747
31	22.176275545581763	28.98927117084179	26.36442718642444	22.470026097152008
32	22.410771694045565	29.787944880797788	25.794576331017787	22.006707094138857
33	22.976209987518754	29.095172657244795	26.020877721605164	21.907739633631287
34	19.25578991162269	27.103216127283503	27.956101312421993	25.68489264867182
35	19.777102585761295	27.757536025416357	27.80418310871292	24.661178280109432
36	23.566862920611705	27.474501695684516	27.433527906302402	21.525107477401377
37	19.02948852103531	29.543993242476578	30.89991048802935	20.526607748458755
38	17.769386906037646	29.038439718100328	29.15757889030371	24.034594485558316
39	19.135390007438318	28.34818895850931	29.228179881239047	23.288241152813324
40	21.41857562500788	26.28626180360317	28.01598608151893	24.279176489870018
41	18.88387397723118	24.85848283513408	27.311867270137043	28.945775917497702
42	22.161146761809906	24.937908949936332	26.75714519850225	26.143799089751507
43	22.163668225771882	25.606727265850555	28.246700034039762	23.9829044743378
44	20.08850338506537	26.842874973209447	28.906062860096572	24.16255878162861
45	19.306849556852708	28.10612841815958	26.84350533919994	25.74351668578777
46	20.469874809314287	28.265611013754587	28.698672449224023	22.565841727707106
47	18.738889799417542	27.25576469698307	29.653676924822552	24.351668578776838
48	19.528738385506625	24.8565917371626	30.425875263177797	25.188794614152975
49	20.392339792483515	23.601533050088882	31.527124648570958	24.47900250885664
50	19.13286854347634	25.368448921443793	29.814420252398545	25.684262282681324
51	18.679005030320603	25.618073853679448	26.89141315447749	28.81150796152246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	80.0
2	127.0
3	205.5
4	284.0
5	242.5
6	201.0
7	222.5
8	244.0
9	294.5
10	345.0
11	364.5
12	384.0
13	448.0
14	512.0
15	497.5
16	483.0
17	444.5
18	406.0
19	423.5
20	441.0
21	477.0
22	513.0
23	744.5
24	976.0
25	995.5
26	1345.5
27	1676.0
28	1763.5
29	1851.0
30	2394.5
31	2938.0
32	3205.5
33	3473.0
34	3917.0
35	4361.0
36	4797.0
37	5233.0
38	6223.5
39	7214.0
40	8261.5
41	9309.0
42	10521.5
43	11734.0
44	13135.5
45	14537.0
46	15035.5
47	15534.0
48	16883.0
49	18232.0
50	16825.5
51	15419.0
52	13038.0
53	10657.0
54	9104.0
55	7551.0
56	6757.0
57	5963.0
58	5159.5
59	4356.0
60	4032.5
61	3709.0
62	3401.5
63	3094.0
64	2691.5
65	2289.0
66	1754.5
67	1220.0
68	1015.0
69	810.0
70	741.0
71	672.0
72	544.5
73	417.0
74	279.0
75	156.0
76	171.0
77	121.5
78	72.0
79	54.5
80	37.0
81	19.5
82	2.0
83	1.0
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	158638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.12594712490072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93676851779122	30.069088112558152
2	10.228575917052863	8.208625928213921
3	3.487550074621004	4.198237496690579
4	1.779907312858377	2.8568186689191744
5	1.1656586285444976	2.33865782473304
6	0.8703165501531693	2.095336552402325
7	0.7854842510407667	2.2062809667292833
8	0.615819652815961	1.9768277461894375
9	0.5514099442306182	1.9913261639708013
>10	5.45126070222292	38.466193471929806
>50	0.0989710156311366	2.753438646478145
>100	0.021993559029141466	1.1907613560433188
>500	0.006283874008326132	1.6484070651420213
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	798	0.5030320604142765	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	663	0.4179326516975756	No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	596	0.3756981303344722	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	558	0.35174422269569716	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	182	0.11472661026992273	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	171	0.10779258437448783	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	168	0.10590148640300559	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCC	166	0.10464075442201742	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.10401038843152334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4614279050416672	0.0
2	0.0	0.0	0.0	1.8482330841286452	0.0
3	0.0	0.0	0.0	2.423757233449741	0.0
4	0.0	0.0	0.0	3.2325167992536468	0.0
5	0.0	0.0	0.0	4.518463419861572	0.0
6	0.0	0.0	0.0	5.1803477098803565	0.0
7	0.0	0.0	0.0	5.8302550460797535	0.0
8	0.0	0.0	0.0	6.918266745672537	0.0
9	0.0	0.0	0.0	7.374021356799758	0.0
10	0.0	0.0	0.0	8.300659362826057	0.0
11	0.0	0.0	0.0	10.104136461629622	0.0
12	0.0	0.0	0.0	11.644120576406662	0.0
13	0.0	0.0	0.0	12.180562034317125	0.0
14	0.0	0.0	0.0	12.355173413683985	0.0
15	0.0	0.0	0.0	12.647032867282745	0.0
16	0.0	0.0	0.0	13.431838525447874	0.0
17	0.0	0.0	0.0	14.664204036863802	0.0
18	0.0	0.0	0.0	16.05857360783671	0.0
19	0.0	0.0	0.0	16.885613787364942	0.0
20	0.0	0.0	0.0	17.769386906037646	0.0
21	0.0	0.0	0.0	18.79751383653349	0.0
22	0.0	0.0	0.0	20.02861861596843	0.0
23	0.0	0.0	0.0	21.10339262976084	0.0
24	0.0	0.0	0.0	22.008598192110338	0.0
25	0.0	0.0	0.0	22.769449942636694	0.0
26	0.0	0.0	0.0	23.410532154969175	0.0
27	0.0	0.0	0.0	24.051614367301656	0.0
28	0.0	0.0	0.0	24.697109141567594	0.0
29	0.0	0.0	0.0	25.415096004740352	0.0
30	0.0	0.0	0.0	26.315889005156393	0.0
31	0.0	0.0	0.0	27.088087343511642	0.0
32	0.0	0.0	0.0	27.89054324941061	0.0
33	0.0	0.0	0.0	28.59087986484953	0.0
34	0.0	0.0	0.0	29.277978794488078	0.0
35	0.0	0.0	0.0	30.067827380577164	0.0
36	0.0	0.0	0.0	30.777619485873497	0.0
37	0.0	0.0	0.0	31.471652441407482	0.0
38	0.0	0.0	0.0	32.17451052080838	0.0
39	0.0	0.0	0.0	32.92968897742029	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTGCG	110	0.0	45.000004	1
TCACGGG	25	3.875563E-5	45.0	3
TGTTTAG	20	7.014889E-4	45.0	1
CGGGTGT	35	1.2030978E-7	45.0	6
CGGCGCA	35	1.2030978E-7	45.0	22
ACCGGCT	35	1.2030978E-7	45.0	20
GTACAGA	35	1.2030978E-7	45.0	9
ACACCGG	35	1.2030978E-7	45.0	18
TTTCGCG	20	7.014889E-4	45.0	1
ATAGCCC	20	7.014889E-4	45.0	24
GTGATAG	20	7.014889E-4	45.0	32
TTCCGGA	20	7.014889E-4	45.0	3
TACGGGG	25	3.875563E-5	45.0	4
TAACGAG	20	7.014889E-4	45.0	21
AGAACGA	25	3.875563E-5	45.0	13
GACCACT	20	7.014889E-4	45.0	25
GCCGATG	20	7.014889E-4	45.0	9
GTCGTGA	20	7.014889E-4	45.0	10
GTGTACA	25	3.875563E-5	45.0	7
TAGGGTA	20	7.014889E-4	45.0	5
>>END_MODULE