SRR2934069.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920834
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	27490	2.9853372051857336	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	26287	2.8546947658318436	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	23552	2.557681406203507	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT	11383	1.2361620009686871	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATGC	7576	0.8227324360308156	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	6024	0.6541895716274594	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGACAACCAT	5829	0.6330131163705944	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5699	0.6188954795326845	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC	5443	0.5910945946826464	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	4916	0.5338638668858882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA	4682	0.5084521205776502	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	4333	0.4705516955281842	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	4030	0.4376467419752094	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	3018	0.3277463690524025	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACAACCATCGAATGC	2340	0.25411746308237965	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACAACCATCGT	1871	0.2031853732594583	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	1787	0.19406320791803952	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1677	0.18211751520903877	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGACAACGAT	1467	0.15931210185549188	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGCCCACCCGACAACCAT	1418	0.15399083873966427	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATAC	1396	0.15160170019786412	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG	1327	0.14410849295312728	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGACCAACGAA	1196	0.12988225890877184	Illumina DpnII expression PCR Primer 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGACAACCA	1171	0.12716732874763528	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAACCATCGTAT	1133	0.12304063490270777	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	1103	0.11978271870934393	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1081	0.11739358016754377	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG	1018	0.11055195616147971	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	1016	0.11033476174858876	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGAGACCACCCGACAACCAT	998	0.10838001203257047	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	958	0.104036123774752	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACATCG	70	0.0	45.000004	38
CGTAATC	125	0.0	45.000004	43
CGACGTC	110	0.0	45.0	45
TTCGTCG	50	2.1827873E-11	45.0	1
CGTAAGC	395	0.0	45.0	43
CGATCGA	150	0.0	45.0	41
CGTCAAT	145	0.0	45.0	42
CGACGTT	65	0.0	44.999996	45
TCGTAAT	60	0.0	44.999996	42
CGGTCAA	65	0.0	44.999996	41
CACCCGT	760	0.0	44.70395	38
ATCGAAT	2345	0.0	44.040512	43
CATCGAA	2865	0.0	43.979057	42
CGTTTTT	3220	0.0	43.951866	1
TCGACGA	210	0.0	43.92857	44
TCGAATG	2030	0.0	43.891624	44
ACGGACG	175	0.0	43.714287	43
TCGAATA	330	0.0	43.636364	44
GGCGAAA	610	0.0	43.524586	32
CCGTCAA	285	0.0	43.421055	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content