##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920834
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.860459105549968	30.0	28.0	31.0	25.0	33.0
2	26.464876405519345	30.0	23.0	31.0	16.0	34.0
3	26.701984288156172	30.0	25.0	31.0	16.0	34.0
4	32.69803134984156	33.0	32.0	35.0	28.0	37.0
5	34.03562205565824	35.0	33.0	35.0	32.0	37.0
6	32.10190544658429	35.0	32.0	35.0	25.0	37.0
7	33.992028965046906	35.0	33.0	35.0	32.0	37.0
8	31.658225043818973	35.0	28.0	37.0	19.0	37.0
9	34.75366026884324	35.0	32.0	39.0	30.0	39.0
10	34.90728730694131	37.0	34.0	39.0	30.0	39.0
11	34.99902805500231	37.0	34.0	39.0	28.0	39.0
12	35.040417708294875	37.0	34.0	39.0	30.0	39.0
13	34.71590210613422	37.0	33.0	39.0	27.0	39.0
14	35.65960857222909	38.0	34.0	40.0	27.0	41.0
15	35.658734364717205	38.0	33.0	40.0	27.0	41.0
16	36.20423225032959	38.0	35.0	40.0	30.0	41.0
17	35.263398180345206	37.0	33.0	40.0	27.0	41.0
18	35.8255375018733	38.0	34.0	40.0	29.0	41.0
19	35.39183175252	37.0	34.0	40.0	27.0	41.0
20	34.29112087520661	36.0	31.0	40.0	25.0	41.0
21	35.03428631001896	37.0	33.0	40.0	27.0	41.0
22	35.08843070520854	37.0	33.0	40.0	27.0	41.0
23	34.06988881818004	36.0	31.0	40.0	24.0	41.0
24	34.45933034618618	37.0	32.0	40.0	24.0	41.0
25	35.090419120058556	37.0	33.0	39.0	27.0	41.0
26	34.767068765923064	37.0	33.0	40.0	27.0	41.0
27	33.76059528644685	36.0	31.0	39.0	23.0	41.0
28	33.52379147598807	36.0	31.0	39.0	23.0	41.0
29	33.7127071763206	36.0	31.0	40.0	23.0	41.0
30	33.635012390941256	36.0	31.0	39.0	23.0	40.0
31	34.0101125718642	37.0	32.0	40.0	24.0	40.0
32	32.50778533373008	36.0	30.0	40.0	16.0	40.0
33	31.69495479098296	35.0	27.0	39.0	12.0	41.0
34	31.686815430359868	36.0	28.0	39.0	12.0	41.0
35	30.86783611378381	35.0	26.0	39.0	12.0	41.0
36	30.903257264610126	35.0	26.0	39.0	10.0	41.0
37	30.101801193266105	35.0	24.0	39.0	7.0	41.0
38	31.330311435068644	35.0	25.0	39.0	12.0	40.0
39	30.98617991950775	35.0	27.0	39.0	11.0	40.0
40	31.07107144175823	35.0	26.0	39.0	13.0	40.0
41	31.572173703403653	35.0	27.0	39.0	17.0	40.0
42	29.921419061416064	35.0	23.0	39.0	7.0	40.0
43	30.33115849327892	35.0	26.0	39.0	7.0	40.0
44	30.506120538555265	35.0	26.0	39.0	8.0	41.0
45	29.867216023735004	35.0	23.0	39.0	7.0	40.0
46	29.96452129265427	35.0	23.0	39.0	7.0	40.0
47	29.956768538086127	35.0	23.0	39.0	8.0	40.0
48	29.392523516725056	35.0	22.0	39.0	7.0	40.0
49	29.391456006185695	35.0	22.0	39.0	7.0	40.0
50	29.40640875554117	35.0	22.0	39.0	7.0	40.0
51	28.67015987680733	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	19.0
12	14.0
13	21.0
14	32.0
15	57.0
16	163.0
17	437.0
18	1158.0
19	3032.0
20	7750.0
21	17971.0
22	30279.0
23	37810.0
24	40886.0
25	39157.0
26	33052.0
27	25832.0
28	22801.0
29	25268.0
30	30529.0
31	37556.0
32	45275.0
33	53876.0
34	65158.0
35	71112.0
36	80259.0
37	92145.0
38	104954.0
39	54221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.47380961172154	2.809409730744086	32.578836142019085	14.137944515515283
2	14.630975832777679	40.090287717438756	32.008266419354634	13.270470030428939
3	12.56339361926254	40.04956376502171	34.21691640404242	13.170126211673331
4	11.86370181813443	3.0160702146097993	68.44719026447764	16.673037702778135
5	44.45155152828848	3.550477067527915	33.80848231060104	18.189489093582555
6	13.570632709044192	36.57260700625737	37.50838913419791	12.348371150500524
7	53.23771711296499	1.360180010729404	42.31348972779024	3.0886131485153676
8	51.9633288953275	32.74075457682927	10.374291131734928	4.92162539610831
9	49.14870649867403	2.9548213901745592	9.555576792342594	38.34089531880882
10	53.12455882384881	18.744855207344646	16.017544964673327	12.11304100413321
11	44.42103571327731	16.14579826548542	23.594806447198952	15.838359574038318
12	17.027064595790335	14.173238607610058	25.97949250353484	42.82020429306477
13	38.35718489977564	15.321545468564365	32.671469559117064	13.649800072542934
14	15.702070079949262	43.4067378050767	23.162698162752463	17.728493952221573
15	11.518254104431417	17.808312898958985	32.861080281570835	37.81235271503876
16	13.394705234602544	20.122410771105322	22.384490581364286	44.09839341292784
17	13.57573677774713	41.6158612735846	27.812721945540673	16.9956800031276
18	13.11745656654728	19.40067373706879	25.582461116770233	41.8994085796137
19	13.562162126941447	22.591259662436443	24.155385226870425	39.69119298375169
20	36.293620782898984	20.26803962494869	30.269082158130566	13.169257434021766
21	17.265978449970355	45.10248318372258	23.685702308993804	13.945836057313263
22	15.181889461075503	16.337146543242323	47.713811609910145	20.76715238577203
23	34.99240905526946	23.5003268775914	25.560415883861804	15.946848183277334
24	17.411064317781488	38.332316139499625	23.46850789610288	20.788111646616002
25	14.14978161101784	48.44553958693967	20.33091740748061	17.07376139456189
26	15.22836906543416	18.490520549849375	45.27015727047438	21.01095311424209
27	37.79063327374967	22.262210126906695	21.41102522278717	18.53613137655647
28	13.572370264347319	40.39816079771164	27.212939574342386	18.81652936359865
29	20.67951444017054	17.722303911454183	42.27689246921812	19.321289179157155
30	14.164550831094422	46.61600244995297	23.853159201332705	15.366287517619897
31	18.001615926431906	19.269705506095562	19.078574422751547	43.65010414472098
32	38.02835255865878	27.62452298677069	18.875714841111428	15.471409613459103
33	34.14882595560112	23.035422236798382	18.38843917579064	24.42731263180986
34	20.639442070992164	20.418229561462763	19.261886507231488	39.680441860313586
35	33.415577617681365	23.685919503406694	19.917270648129847	22.981232230782094
36	18.399407493641633	41.68015081980031	18.32371524074915	21.59672644580891
37	14.030541878340722	28.21811531720158	24.50843474502462	33.24290805943308
38	15.495083804464214	23.95784690834613	18.39853871599007	42.148530571199586
39	17.33884717549526	40.49014263157095	19.74199475692687	22.429015436006924
40	14.235030418077526	26.766713653057987	22.34539558704392	36.65286034182056
41	14.662034633821081	25.230497570680495	19.477560559232174	40.629907236266256
42	23.18257145153198	19.99372308146745	25.87132968591516	30.952375781085408
43	22.15708803106749	35.278019708221024	19.69855587434869	22.8663363863628
44	19.48223023910933	24.062317420946663	32.61195828998495	23.84349404995906
45	20.51509826961211	18.306774076543654	25.284144590664546	35.89398306317968
46	28.634259812300588	33.4577133337822	20.126428867743805	17.781597986173402
47	13.2749225158932	38.729999109502906	30.414711011973928	17.580367362629964
48	18.266158721333053	27.031256447959134	25.76533881242439	28.937246018283425
49	24.06720429523671	18.63636659810563	33.44283551649917	23.853593590158486
50	21.635821440129273	29.701335962833692	25.277520161071376	23.38532243596566
51	19.15795898066318	19.420221234228972	31.6883390491663	29.733480735941548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	446.5
2	686.0
3	4980.5
4	9275.0
5	6484.0
6	3693.0
7	3589.0
8	3485.0
9	3516.5
10	3548.0
11	3625.0
12	3702.0
13	3732.5
14	3763.0
15	3478.0
16	3193.0
17	3030.0
18	2867.0
19	2896.0
20	2925.0
21	3023.5
22	3122.0
23	3565.5
24	4009.0
25	4438.5
26	5828.0
27	6788.0
28	7811.5
29	8835.0
30	10231.5
31	11628.0
32	12822.5
33	14017.0
34	16738.5
35	19460.0
36	20204.0
37	20948.0
38	22924.5
39	24901.0
40	26159.5
41	27418.0
42	30282.5
43	33147.0
44	37637.0
45	42127.0
46	99159.5
47	156192.0
48	123466.5
49	90741.0
50	88349.5
51	85958.0
52	85400.5
53	84843.0
54	81525.5
55	78208.0
56	66469.5
57	54731.0
58	46129.5
59	37528.0
60	31341.0
61	25154.0
62	20943.5
63	16733.0
64	14204.5
65	11676.0
66	9669.5
67	7663.0
68	6290.5
69	4918.0
70	4071.5
71	3225.0
72	2684.5
73	2144.0
74	1782.5
75	995.0
76	569.0
77	441.5
78	314.0
79	222.5
80	131.0
81	80.5
82	30.0
83	23.5
84	17.0
85	17.0
86	17.0
87	8.5
88	0.0
89	3.5
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	920834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.543220050276513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28433685736796	21.650552900158115
2	7.687157322333952	4.542067606696127
3	3.445545959985019	3.05377567467536
4	2.4132090580310366	2.851758649149257
5	1.8911668220172853	2.793557878731939
6	1.6059547714667382	2.8467045122540027
7	1.4195957869652316	2.935760150173151
8	1.2712923898875013	3.00464566581507
9	1.110989244934558	2.9539979762932975
>10	5.6307584396483294	24.9565561958641
>50	0.1289683906314421	2.683333564307052
>100	0.08747420833346664	5.219231016831523
>500	0.012709927706571929	2.6983531482732146
>1k	0.00747642806268937	4.636224170773525
>5k	0.0018691070156723426	3.3762269920132795
>10k+	0.001495285612537874	9.797253897990972
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	27490	2.9853372051857336	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	26287	2.8546947658318436	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	23552	2.557681406203507	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT	11383	1.2361620009686871	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATGC	7576	0.8227324360308156	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	6024	0.6541895716274594	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGACAACCAT	5829	0.6330131163705944	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5699	0.6188954795326845	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC	5443	0.5910945946826464	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	4916	0.5338638668858882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA	4682	0.5084521205776502	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	4333	0.4705516955281842	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCATCGAATGC	4030	0.4376467419752094	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	3018	0.3277463690524025	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACAACCATCGAATGC	2340	0.25411746308237965	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACAACCATCGT	1871	0.2031853732594583	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	1787	0.19406320791803952	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1677	0.18211751520903877	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGACAACGAT	1467	0.15931210185549188	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGCCCACCCGACAACCAT	1418	0.15399083873966427	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCAACCATCGAATAC	1396	0.15160170019786412	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG	1327	0.14410849295312728	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGACCAACGAA	1196	0.12988225890877184	Illumina DpnII expression PCR Primer 2 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGACAACCA	1171	0.12716732874763528	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAACCATCGTAT	1133	0.12304063490270777	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	1103	0.11978271870934393	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	1081	0.11739358016754377	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG	1018	0.11055195616147971	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	1016	0.11033476174858876	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGAGACCACCCGACAACCAT	998	0.10838001203257047	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	958	0.104036123774752	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11120353940015247	0.0
2	0.0	0.0	0.0	0.8693206375959185	0.0
3	0.0	0.0	0.0	1.0810851901645682	0.0
4	0.0	0.0	0.0	2.0318537325945827	0.0
5	0.0	0.0	0.0	5.702982296483405	0.0
6	0.0	0.0	0.0	6.480212503013573	0.0
7	1.085972064454614E-4	0.0	0.0	7.364953943924746	0.0
8	1.085972064454614E-4	0.0	0.0	8.3626364795392	0.0
9	1.085972064454614E-4	0.0	0.0	8.603396486228789	0.0
10	1.085972064454614E-4	0.0	0.0	11.865222179024666	0.0
11	1.085972064454614E-4	0.0	0.0	12.280823688091447	0.0
12	1.085972064454614E-4	0.0	0.0	16.334866001906967	0.0
13	1.085972064454614E-4	0.0	0.0	16.651752650314823	0.0
14	1.085972064454614E-4	0.0	0.0	16.863408605677026	0.0
15	1.085972064454614E-4	0.0	0.0	17.570593614049873	0.0
16	1.085972064454614E-4	0.0	0.0	17.974683819233434	0.0
17	1.085972064454614E-4	0.0	0.0	18.262466416313906	0.0
18	1.085972064454614E-4	0.0	0.0	18.495516021345868	0.0
19	1.085972064454614E-4	0.0	0.0	20.28660974725086	0.0
20	1.085972064454614E-4	0.0	0.0	20.60946924201322	0.0
21	1.085972064454614E-4	0.0	0.0	20.832527904052196	0.0
22	1.085972064454614E-4	0.0	0.0	21.11618380728774	0.0
23	1.085972064454614E-4	0.0	0.0	21.38615646251116	0.0
24	1.085972064454614E-4	0.0	0.0	21.673178879146512	0.0
25	1.085972064454614E-4	0.0	0.0	21.868871045161235	0.0
26	1.085972064454614E-4	0.0	0.0	22.07401116813671	0.0
27	1.085972064454614E-4	0.0	0.0	22.34083450437321	0.0
28	1.085972064454614E-4	0.0	0.0	22.544237072045558	0.0
29	1.085972064454614E-4	0.0	0.0	22.795531007760356	0.0
30	1.085972064454614E-4	0.0	0.0	23.012182434619053	0.0
31	1.085972064454614E-4	0.0	0.0	23.23882480447073	0.0
32	1.085972064454614E-4	0.0	0.0	23.45699659221966	0.0
33	1.085972064454614E-4	0.0	0.0	23.66952132523343	0.0
34	1.085972064454614E-4	0.0	0.0	23.900290388930035	0.0
35	1.085972064454614E-4	0.0	0.0	24.13920424311005	0.0
36	1.085972064454614E-4	0.0	0.0	24.363783266039263	0.0
37	2.171944128909228E-4	0.0	0.0	24.584452789536442	0.0
38	2.171944128909228E-4	0.0	0.0	24.83238021185143	0.0
39	2.171944128909228E-4	0.0	0.0	25.0699908995541	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACATCG	70	0.0	45.000004	38
CGTAATC	125	0.0	45.000004	43
CGACGTC	110	0.0	45.0	45
TTCGTCG	50	2.1827873E-11	45.0	1
CGTAAGC	395	0.0	45.0	43
CGATCGA	150	0.0	45.0	41
CGTCAAT	145	0.0	45.0	42
CGACGTT	65	0.0	44.999996	45
TCGTAAT	60	0.0	44.999996	42
CGGTCAA	65	0.0	44.999996	41
CACCCGT	760	0.0	44.70395	38
ATCGAAT	2345	0.0	44.040512	43
CATCGAA	2865	0.0	43.979057	42
CGTTTTT	3220	0.0	43.951866	1
TCGACGA	210	0.0	43.92857	44
TCGAATG	2030	0.0	43.891624	44
ACGGACG	175	0.0	43.714287	43
TCGAATA	330	0.0	43.636364	44
GGCGAAA	610	0.0	43.524586	32
CCGTCAA	285	0.0	43.421055	41
>>END_MODULE