SRR2934067.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399499
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	2050	0.5131427112458354	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	1769	0.4428046127775038	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	1586	0.3969972390419	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1459	0.3652074222964263	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1424	0.35644644917759494	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1195	0.2991246536286699	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	1099	0.275094555931304	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	836	0.20926210078122848	No Hit
TTCTGTCGGGCCTCACCCTTGCAACCTCCCCACAAAAATAAAACACAAATA	506	0.12665863994653304	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.027754E-4	45.0	8
CCGATCG	20	7.027754E-4	45.0	40
GTTTACG	20	7.027754E-4	45.0	1
CACGCTA	25	3.88622E-5	45.0	35
GAATCGG	20	7.027754E-4	45.0	22
ACTTCGA	20	7.027754E-4	45.0	29
TTCGTAG	25	3.88622E-5	45.0	1
GCCGATC	35	1.2092642E-7	45.0	9
CTACGAA	25	3.88622E-5	45.0	11
TACGAAT	25	3.88622E-5	45.0	12
GTCGTCG	25	3.88622E-5	45.0	1
GCGATAG	20	7.027754E-4	45.0	9
TATAGCG	35	1.2092642E-7	45.0	38
ATGCGCT	20	7.027754E-4	45.0	12
GACGCTA	20	7.027754E-4	45.0	21
GCTACGA	25	3.88622E-5	45.0	10
TTGCACG	20	7.027754E-4	45.0	1
CATCCCG	20	7.027754E-4	45.0	36
AAAGCGA	20	7.027754E-4	45.0	28
CGCCCAC	25	3.88622E-5	45.0	34