##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399499
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88404726920468	31.0	30.0	31.0	25.0	34.0
2	30.07085124117958	31.0	30.0	33.0	26.0	34.0
3	30.147517265374884	31.0	30.0	33.0	25.0	34.0
4	33.93433775804195	35.0	33.0	37.0	28.0	37.0
5	34.383905842067186	35.0	35.0	37.0	32.0	37.0
6	33.83645516008801	35.0	35.0	37.0	30.0	37.0
7	34.47072708567481	35.0	35.0	37.0	32.0	37.0
8	34.587230506209025	35.0	35.0	37.0	32.0	37.0
9	35.91812745463693	37.0	35.0	39.0	31.0	39.0
10	35.046282969419195	37.0	34.0	39.0	29.0	39.0
11	35.21414321437601	37.0	34.0	39.0	30.0	39.0
12	35.23009819799299	37.0	34.0	39.0	30.0	39.0
13	35.25243367317565	37.0	34.0	39.0	30.0	39.0
14	35.944355305019535	38.0	34.0	40.0	29.0	41.0
15	36.17093409495393	38.0	34.0	40.0	30.0	41.0
16	36.36068676016711	38.0	35.0	40.0	31.0	41.0
17	36.06112656101767	38.0	34.0	40.0	30.0	41.0
18	36.21416323945742	38.0	35.0	40.0	30.0	41.0
19	36.215522441858425	38.0	34.0	40.0	30.0	41.0
20	36.214513678382175	38.0	34.0	40.0	30.0	41.0
21	35.92800983231498	38.0	34.0	40.0	29.0	41.0
22	36.10620802555201	38.0	34.0	40.0	30.0	41.0
23	36.14162738830385	38.0	34.0	40.0	30.0	41.0
24	36.06210779000698	38.0	34.0	40.0	30.0	41.0
25	35.57847704249573	38.0	34.0	40.0	28.0	41.0
26	35.65944595605996	38.0	34.0	40.0	29.0	41.0
27	35.702857829431366	38.0	34.0	40.0	29.0	41.0
28	35.66718815316184	38.0	34.0	40.0	29.0	41.0
29	35.81103331923234	38.0	34.0	40.0	29.0	41.0
30	35.29870913318932	38.0	34.0	40.0	27.0	41.0
31	35.47254185867799	38.0	34.0	40.0	29.0	41.0
32	35.526138989083826	38.0	34.0	40.0	29.0	41.0
33	35.577911333945764	38.0	34.0	40.0	29.0	41.0
34	35.54964843466442	38.0	34.0	40.0	28.0	41.0
35	35.55381865786898	38.0	34.0	40.0	28.0	41.0
36	35.36807601520905	38.0	34.0	40.0	27.0	41.0
37	35.42648166828953	38.0	34.0	40.0	28.0	41.0
38	35.378454013652096	38.0	34.0	40.0	28.0	41.0
39	35.264676507325426	38.0	34.0	40.0	27.0	41.0
40	35.24458384126118	37.0	34.0	40.0	27.0	41.0
41	35.115049098996494	37.0	33.0	40.0	27.0	41.0
42	35.32633623613576	38.0	34.0	40.0	28.0	41.0
43	35.308599020272894	38.0	34.0	40.0	28.0	41.0
44	35.286979441750795	38.0	34.0	40.0	27.0	41.0
45	35.36180315845596	38.0	34.0	40.0	28.0	41.0
46	35.23957256463721	37.0	34.0	40.0	28.0	41.0
47	35.15606046573333	37.0	34.0	40.0	27.0	41.0
48	35.158984127619846	37.0	34.0	40.0	27.0	41.0
49	35.203610021551995	37.0	34.0	40.0	28.0	41.0
50	34.98761198400997	37.0	34.0	40.0	27.0	41.0
51	33.909481625736234	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	2.0
13	3.0
14	13.0
15	24.0
16	40.0
17	74.0
18	159.0
19	302.0
20	596.0
21	997.0
22	1577.0
23	2214.0
24	2889.0
25	3761.0
26	4859.0
27	6264.0
28	7871.0
29	10104.0
30	13052.0
31	16665.0
32	21235.0
33	27410.0
34	36855.0
35	38994.0
36	46757.0
37	58911.0
38	68581.0
39	29282.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.33147767578893	4.843316253607643	56.0224180786435	13.802787991959931
2	18.548481973672022	6.387250030663407	54.18061121554747	20.883656780117096
3	20.301177224473655	6.074608447079967	52.61815423818332	21.006060090263055
4	18.74547871208689	4.64682014222814	49.92753423663138	26.680166909053586
5	18.823075902567965	5.344944543040158	51.37159292013247	24.46038663425941
6	19.76625723718958	8.194013001284109	54.601388238769054	17.438341522757252
7	83.46078463275252	1.8743476203945442	10.51216648852688	4.152701258326053
8	85.51886237512485	4.00626785048273	5.670852743060683	4.8040170313317425
9	80.03949947309005	4.581988941148789	8.227054385617986	7.15145720014318
10	40.45316759240949	25.076157887754412	18.767506301642808	15.703168218193287
11	30.63862487765927	27.801321154746322	22.38528757268479	19.17476639490962
12	24.51044933779559	23.500183980435494	29.49919774517583	22.490168936593083
13	23.983539383077304	25.843368819446354	31.948765829201076	18.224325968275267
14	21.17627328228606	27.85438762049467	29.883428994816008	21.08591010240326
15	18.878895817010807	25.716960493017503	34.961289014490646	20.44285467548104
16	21.746487475563143	24.213327192308366	30.454143815128447	23.58604151700004
17	21.17852610394519	26.650379600449565	29.259147081719856	22.91194721388539
18	22.36075684795206	23.826092180456023	30.585308098393238	23.227842873198682
19	23.07139692464812	28.13123437104974	27.674662514799785	21.122706189502352
20	25.627097940170064	26.36502219029334	29.438371560379373	18.56950830915722
21	24.726720217071883	28.416591781205963	27.342246163319555	19.5144418384026
22	21.950993619508434	25.444118758745326	28.58680497322897	24.018082648517268
23	21.694422263885514	28.644126768777895	27.27290931892195	22.388541648414638
24	22.647115512179003	25.93172949118771	28.547005123917707	22.874149872715577
25	20.875646747551308	29.254391124883917	26.120465883519106	23.749496244045666
26	20.731966788402474	28.113462111294396	28.36027123972776	22.79429986057537
27	21.13922688166929	27.912460356596636	27.48567580895071	23.46263695278336
28	18.9051787363673	26.837864425192553	30.234118233086942	24.022838605353204
29	21.48541047662197	25.01357950833419	29.793065814933207	23.70794420011064
30	22.045361815674134	27.408579245504995	27.56677738867932	22.979281550141554
31	21.995799739173314	26.343495227772785	27.576038988833513	24.084666044220384
32	23.09342451420404	27.87441270190914	27.560018923701936	21.472143860184882
33	22.933474176405948	25.782042007614535	27.891684334629126	23.39279948135039
34	21.306185997962448	25.23986292831772	29.023852375099814	24.43009869862002
35	20.468636967802173	25.107947704499882	30.065156608652337	24.358258719045605
36	22.95875584169172	26.070403179982925	27.922973524339234	23.047867453986118
37	18.91644284466294	27.61759103276854	30.17229079421976	23.293675328348755
38	19.82257777866778	27.396814510173996	28.53098505878613	24.249622652372096
39	21.249364829448886	26.164521062630946	30.570789914367747	22.015324193552424
40	22.451870968387905	24.321712945464196	29.907709406031053	23.318706680116847
41	19.381275046996365	24.783040758550083	30.097447052433175	25.73823714202038
42	20.447610632316977	24.530975046245423	29.602326914460363	25.419087406977237
43	20.9242075699814	24.218083149144302	29.414091149164328	25.443618131709968
44	19.778522599555945	24.571776149627407	29.01183732625113	26.63786392456552
45	21.566762369868258	24.034603340684207	27.702697628780047	26.695936660667485
46	22.642609868860745	25.029599573465767	28.7069554617158	23.620835095957688
47	19.460123805065844	24.467645726272156	31.36603596003995	24.706194508622048
48	19.187282070793668	24.27290180951642	31.244383590447033	25.295432529242877
49	20.970765884270048	22.060630940252665	33.145514757233435	23.823088418243852
50	21.419578021471892	22.74348621648615	29.82685813982012	26.010077622221832
51	19.638347029654643	22.76651506011279	29.007581996450554	28.587555913782015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	118.5
2	202.0
3	367.0
4	532.0
5	416.5
6	301.0
7	335.5
8	370.0
9	453.0
10	536.0
11	649.5
12	763.0
13	805.5
14	848.0
15	874.0
16	900.0
17	901.0
18	902.0
19	929.0
20	956.0
21	1072.5
22	1189.0
23	1380.5
24	1572.0
25	2115.5
26	2965.5
27	3272.0
28	4011.0
29	4750.0
30	5717.0
31	6684.0
32	7098.0
33	7512.0
34	9010.0
35	10508.0
36	11697.0
37	12886.0
38	14510.5
39	16135.0
40	18063.0
41	19991.0
42	22558.5
43	25126.0
44	28073.0
45	31020.0
46	38036.5
47	45053.0
48	45817.5
49	46582.0
50	44618.5
51	42655.0
52	36742.0
53	30829.0
54	27201.5
55	23574.0
56	20788.0
57	18002.0
58	15999.5
59	13997.0
60	12195.0
61	10393.0
62	9117.5
63	7842.0
64	6259.5
65	4677.0
66	3654.0
67	2631.0
68	2060.0
69	1489.0
70	1170.5
71	852.0
72	668.5
73	485.0
74	460.0
75	332.0
76	229.0
77	158.5
78	88.0
79	59.0
80	30.0
81	16.5
82	3.0
83	2.0
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.83772106699769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65804645578919	28.995483836568965
2	10.647580664984606	8.270555358100598
3	3.7565933430821623	4.376925732622961
4	1.891502383656999	2.938465678961271
5	1.1869257918154619	2.3048746414877135
6	0.8503831432991262	1.9816165991716914
7	0.6732756419380974	1.8303944107956944
8	0.5768883970776487	1.7924024521991304
9	0.5093074369587254	1.7802306156555439
>10	5.08851085570024	36.61381907405165
>50	0.10688941398553195	2.786461245682764
>100	0.04823058923737418	3.3104415126307405
>500	0.0013035294388479507	0.3397012165430841
>1k	0.004562353035967828	2.6786276255282533
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	2050	0.5131427112458354	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	1769	0.4428046127775038	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	1586	0.3969972390419	TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1459	0.3652074222964263	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1424	0.35644644917759494	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1195	0.2991246536286699	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	1099	0.275094555931304	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT	836	0.20926210078122848	No Hit
TTCTGTCGGGCCTCACCCTTGCAACCTCCCCACAAAAATAAAACACAAATA	506	0.12665863994653304	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4330423855879489	0.0
2	0.0	0.0	0.0	1.5872380156145571	0.0
3	0.0	0.0	0.0	1.9747233409845832	0.0
4	0.0	0.0	0.0	2.7038866179890313	0.0
5	0.0	0.0	0.0	4.011774747871709	0.0
6	0.0	0.0	0.0	4.620036595836285	0.0
7	0.0	0.0	0.0	5.260088260546334	0.0
8	0.0	0.0	0.0	6.328176040490715	0.0
9	0.0	0.0	0.0	6.710655095507122	0.0
10	0.0	0.0	0.0	8.039319247357316	0.0
11	0.0	0.0	0.0	9.644579836244898	0.0
12	0.0	0.0	0.0	11.64984142638655	0.0
13	0.0	0.0	0.0	12.153722537478192	0.0
14	0.0	0.0	0.0	12.306664096781219	0.0
15	0.0	0.0	0.0	12.635826372531596	0.0
16	0.0	0.0	0.0	13.312173497305375	0.0
17	0.0	0.0	0.0	14.40404106142944	0.0
18	0.0	0.0	0.0	15.500414268871761	0.0
19	0.0	0.0	0.0	16.49215642592347	0.0
20	0.0	0.0	0.0	17.255362341332518	0.0
21	0.0	0.0	0.0	18.333212348466454	0.0
22	0.0	0.0	0.0	19.482401708139445	0.0
23	0.0	0.0	0.0	20.682154398383975	0.0
24	0.0	0.0	0.0	21.508939947283974	0.0
25	0.0	0.0	0.0	22.353998382974677	0.0
26	0.0	0.0	0.0	23.058130308211034	0.0
27	0.0	0.0	0.0	23.76451505510652	0.0
28	0.0	0.0	0.0	24.520461878502825	0.0
29	0.0	0.0	0.0	25.272653999133915	0.0
30	0.0	0.0	0.0	26.127474662014173	0.0
31	0.0	0.0	0.0	26.94099359447708	0.0
32	0.0	0.0	0.0	27.71396173707569	0.0
33	0.0	0.0	0.0	28.523225339738023	0.0
34	0.0	0.0	0.0	29.28543000107635	0.0
35	2.5031351768089533E-4	0.0	0.0	30.151264458734566	0.0
36	2.5031351768089533E-4	0.0	0.0	30.928738244651427	0.0
37	2.5031351768089533E-4	0.0	0.0	31.745010625808824	0.0
38	2.5031351768089533E-4	0.0	0.0	32.56278488807231	0.0
39	2.5031351768089533E-4	0.0	0.0	33.39833141009114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.027754E-4	45.0	8
CCGATCG	20	7.027754E-4	45.0	40
GTTTACG	20	7.027754E-4	45.0	1
CACGCTA	25	3.88622E-5	45.0	35
GAATCGG	20	7.027754E-4	45.0	22
ACTTCGA	20	7.027754E-4	45.0	29
TTCGTAG	25	3.88622E-5	45.0	1
GCCGATC	35	1.2092642E-7	45.0	9
CTACGAA	25	3.88622E-5	45.0	11
TACGAAT	25	3.88622E-5	45.0	12
GTCGTCG	25	3.88622E-5	45.0	1
GCGATAG	20	7.027754E-4	45.0	9
TATAGCG	35	1.2092642E-7	45.0	38
ATGCGCT	20	7.027754E-4	45.0	12
GACGCTA	20	7.027754E-4	45.0	21
GCTACGA	25	3.88622E-5	45.0	10
TTGCACG	20	7.027754E-4	45.0	1
CATCCCG	20	7.027754E-4	45.0	36
AAAGCGA	20	7.027754E-4	45.0	28
CGCCCAC	25	3.88622E-5	45.0	34
>>END_MODULE