##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.041965613349944	31.0	30.0	31.0	26.0	34.0
2	30.155598054906115	31.0	30.0	33.0	26.0	34.0
3	30.220631517234693	31.0	30.0	34.0	25.0	34.0
4	34.01038265963457	35.0	33.0	37.0	28.0	37.0
5	34.46213709744145	35.0	35.0	37.0	32.0	37.0
6	33.902293782933384	35.0	35.0	37.0	30.0	37.0
7	34.43448782897925	35.0	35.0	37.0	32.0	37.0
8	34.426930141626855	35.0	35.0	37.0	31.0	37.0
9	35.933846004732715	37.0	35.0	39.0	31.0	39.0
10	35.339743695820225	37.0	34.0	39.0	30.0	39.0
11	35.48071808667819	37.0	35.0	39.0	30.0	39.0
12	35.44618959701002	37.0	35.0	39.0	30.0	39.0
13	35.37154177294788	37.0	35.0	39.0	30.0	39.0
14	35.97966729154896	38.0	34.0	40.0	27.0	41.0
15	36.260932879131374	38.0	35.0	40.0	30.0	41.0
16	36.47638725051003	38.0	35.0	40.0	31.0	41.0
17	36.23113710453343	38.0	35.0	40.0	30.0	41.0
18	36.37718698952514	38.0	35.0	40.0	30.0	41.0
19	36.35041003467978	38.0	35.0	40.0	30.0	41.0
20	36.291504043610956	38.0	34.0	40.0	30.0	41.0
21	36.071730656031846	38.0	34.0	40.0	30.0	41.0
22	36.28532692848683	38.0	34.0	40.0	30.0	41.0
23	36.3153401904906	38.0	34.0	40.0	30.0	41.0
24	36.2528929370965	38.0	34.0	40.0	30.0	41.0
25	35.8499927898197	38.0	34.0	40.0	29.0	41.0
26	35.908849137024156	38.0	34.0	40.0	30.0	41.0
27	35.895698240952406	38.0	34.0	40.0	30.0	41.0
28	35.80100611565129	38.0	34.0	40.0	29.0	41.0
29	35.922654859306924	38.0	34.0	40.0	30.0	41.0
30	35.49767737634541	38.0	34.0	40.0	28.0	41.0
31	35.623522208537324	38.0	34.0	40.0	29.0	41.0
32	35.612858233671304	38.0	34.0	40.0	29.0	41.0
33	35.615751761766184	38.0	34.0	40.0	29.0	41.0
34	35.569209456919765	38.0	34.0	40.0	28.0	41.0
35	35.52903102268725	38.0	34.0	40.0	28.0	41.0
36	35.35834832499238	38.0	34.0	40.0	27.0	41.0
37	35.36509279856647	38.0	34.0	40.0	28.0	41.0
38	35.28915659802417	38.0	34.0	40.0	27.0	41.0
39	35.21109280329446	37.0	34.0	40.0	27.0	41.0
40	35.155449123313	37.0	34.0	40.0	27.0	41.0
41	35.0106001470404	37.0	33.0	40.0	26.0	41.0
42	35.25067787514804	37.0	34.0	40.0	28.0	41.0
43	35.23481784247765	37.0	34.0	40.0	28.0	41.0
44	35.20082361534988	37.0	34.0	40.0	28.0	41.0
45	35.23240893305879	37.0	34.0	40.0	28.0	41.0
46	35.13541664204173	37.0	34.0	40.0	28.0	41.0
47	35.04343838132634	37.0	34.0	40.0	27.0	41.0
48	35.00859784451069	37.0	34.0	40.0	27.0	41.0
49	35.04151645457705	37.0	34.0	40.0	27.0	41.0
50	34.84131693352214	37.0	33.0	40.0	27.0	41.0
51	33.71493311080274	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	3.0
13	11.0
14	16.0
15	30.0
16	46.0
17	109.0
18	177.0
19	374.0
20	679.0
21	1177.0
22	1878.0
23	2600.0
24	3264.0
25	4281.0
26	5284.0
27	6316.0
28	8111.0
29	10093.0
30	13152.0
31	16799.0
32	21716.0
33	28844.0
34	39225.0
35	39786.0
36	48200.0
37	60912.0
38	73032.0
39	36891.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.98498627701749	4.293485070198788	51.15752943762958	17.563999215154144
2	18.588081217362117	7.587237271667774	52.5806535496545	21.24402796131561
3	21.175235749255933	7.451071243673363	50.91711129445194	20.45658171261876
4	19.991820582346172	5.129629585851971	48.49519991111384	26.383349920688016
5	21.28161545862657	5.724410360910894	48.37298144501469	24.620992735447846
6	22.22815847267105	9.130215856250281	51.0057610522608	17.635864618817862
7	82.57807679669419	2.4429509258580704	10.998952750861084	3.9800195265866534
8	84.39882462241113	5.6390701940602295	5.8196793006361505	4.142425882892488
9	79.3304224692858	5.031996652585145	7.390316609655022	8.247264268474018
10	43.590149711710986	23.85033084089614	16.626912175275937	15.932607272116933
11	33.06163167562226	23.95883814445419	23.482493445827906	19.497036734095644
12	27.977863564476742	21.131265469382736	29.06104540522395	21.829825560916568
13	26.44150416181063	22.862417939874188	30.76973993707049	19.926337961244688
14	21.02441296130379	26.09446990990821	31.44584208995941	21.435275038828593
15	18.773891109729487	23.490294624515084	36.36862224092404	21.36719202483139
16	23.344672622354395	22.31940862337564	30.566200093141344	23.769718661128618
17	24.13259166975956	23.945836179975558	28.22017290248763	23.701399247777253
18	24.345351088500827	22.66691567398638	29.8704767938574	23.117256443655396
19	24.671109398529122	24.258828924879378	27.416887897062264	23.653173779529236
20	26.141040582677128	26.430866190873566	28.709519565592544	18.71857366085676
21	25.893294059520628	26.468217288830363	28.580209118868687	19.05827953278032
22	23.916522660060092	23.247748887150042	30.06125107266207	22.7744773801278
23	23.2869911799401	25.180313607383226	29.290825577464524	22.241869635212154
24	23.42457560405945	25.242486637526508	28.676187256656416	22.65675050175763
25	23.327651868855096	27.387337977792647	26.05522761711815	23.22978253623411
26	21.56482188490661	26.35521839754334	27.592532617200888	24.487427100349162
27	22.466212622307115	26.139149387843872	28.773347391214926	22.621290598634083
28	19.022819629656773	27.206019673154252	30.489843101748647	23.281317595440328
29	22.290095103460178	25.711030157465608	29.080430152264825	22.91844458680939
30	23.162172320945217	26.71407261715361	28.2769087474853	21.84684631441587
31	23.871843182124426	25.839395006772843	27.368426029460085	22.920335781642645
32	27.39986714356296	26.633460437386088	25.017670851723235	20.94900156732772
33	25.618834409344394	25.755000437338804	25.160928860342352	23.46523629297445
34	22.452974258474327	26.276733811963226	27.452347800185812	23.817944129376638
35	23.197395824714604	27.66179762796888	26.136548994948143	23.00425755236837
36	23.706127234860393	29.5903435591814	25.312933645065282	21.390595560892926
37	22.075444489885655	28.798169323401407	26.716909409403495	22.409476777309443
38	24.482462713911865	26.388077907771155	25.957358284497168	23.172101093819812
39	23.968057719266312	26.690905480446226	25.598504064886896	23.742532735400566
40	25.820246659086127	24.38246578710347	26.343871228543804	23.4534163252666
41	20.842385458602926	25.98667180441263	26.019767713994607	27.151175022989836
42	22.43595350497502	25.938919134872922	27.732481034861813	23.89264632529024
43	21.74874058244073	26.788774813067214	27.61049896811682	23.851985636375243
44	22.7354714866919	25.23492185819348	27.80647403271294	24.223132622401675
45	22.288440307981077	24.55598291305468	26.52282553964063	26.632751239323614
46	23.20945219177661	26.398006680645747	26.584525771075597	23.808015356502047
47	20.405046653412544	26.846692654835667	29.951088973624923	22.797171718126865
48	21.751577374690612	26.71241782167451	28.09937283251342	23.436631971121454
49	23.006621545909937	25.116722181115	29.06151820393227	22.815138069042796
50	22.51041930153447	25.73821608319366	26.842910265169156	24.908454350102716
51	21.541654748199228	25.388817837749666	26.236073123048225	26.83345429100288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	57.0
1	226.0
2	395.0
3	651.0
4	907.0
5	686.5
6	466.0
7	490.5
8	515.0
9	537.5
10	560.0
11	600.5
12	641.0
13	654.0
14	667.0
15	719.0
16	771.0
17	770.5
18	770.0
19	884.0
20	998.0
21	1112.0
22	1226.0
23	1327.0
24	1428.0
25	2099.5
26	3255.5
27	3740.0
28	4155.5
29	4571.0
30	5583.5
31	6596.0
32	8016.0
33	9436.0
34	11128.5
35	12821.0
36	13388.0
37	13955.0
38	15058.5
39	16162.0
40	18214.5
41	20267.0
42	22167.5
43	24068.0
44	25612.5
45	27157.0
46	32975.0
47	38793.0
48	37983.0
49	37173.0
50	36233.0
51	35293.0
52	32248.5
53	29204.0
54	27677.0
55	26150.0
56	25148.0
57	24146.0
58	22659.0
59	21172.0
60	19427.5
61	17683.0
62	15698.0
63	13713.0
64	11992.0
65	10271.0
66	8446.5
67	6622.0
68	5469.0
69	4316.0
70	3614.0
71	2912.0
72	2436.5
73	1961.0
74	1597.5
75	960.5
76	687.0
77	553.5
78	420.0
79	310.0
80	200.0
81	140.5
82	81.0
83	54.0
84	27.0
85	15.0
86	3.0
87	4.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	423013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.370656487888915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.33535592286825	25.20584326841974
2	8.669218096457733	5.95933434423878
3	3.0962521677904182	3.192606589770176
4	1.7304732341800144	2.3791000437394976
5	1.290067950183403	2.217024118089437
6	1.1297579399382836	2.3298313240850272
7	1.008782248594107	2.427075569625575
8	0.9668231681234019	2.658427759688154
9	0.9073428655776828	2.806737295455658
>10	7.751142780452019	44.46724493365862
>50	0.0793050505766646	1.8372623637935095
>100	0.028521991874063582	1.7475989871117596
>500	0.004173950030350768	0.9621478073796307
>1k	0.002782633353567179	1.8097655949444396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	2676	0.6326046717240369	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	2393	0.565703654497616	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1478	0.34939824544399345	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	1022	0.24160013994841767	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	858	0.20283064586667549	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	842	0.19904825620016406	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	691	0.16335195372246245	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	573	0.13545682993194064	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	532	0.1257644564115051	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	528	0.12481885899487723	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18226390205501958	0.0
2	0.0	0.0	0.0	0.7895738428842612	0.0
3	0.0	0.0	0.0	1.037083966686603	0.0
4	0.0	0.0	0.0	1.4538560280653314	0.0
5	0.0	0.0	0.0	2.3876334769853407	0.0
6	0.0	0.0	0.0	2.733722131471137	0.0
7	0.0	0.0	0.0	3.162786959266027	0.0
8	0.0	0.0	0.0	3.802956410323087	0.0
9	0.0	0.0	0.0	4.031081787084558	0.0
10	0.0	0.0	0.0	5.13364837487264	0.0
11	0.0	0.0	0.0	6.035984709689773	0.0
12	0.0	0.0	0.0	7.590546862625971	0.0
13	0.0	0.0	0.0	7.932380328736942	0.0
14	0.0	0.0	0.0	8.076820334126847	0.0
15	0.0	0.0	0.0	8.387685484843255	0.0
16	0.0	0.0	0.0	8.813677121034106	0.0
17	0.0	0.0	0.0	9.372525194261168	0.0
18	0.0	0.0	0.0	10.007021060818461	0.0
19	0.0	0.0	0.0	10.693051986581972	0.0
20	0.0	0.0	0.0	11.201546997373603	0.0
21	0.0	0.0	0.0	11.8294236820145	0.0
22	0.0	0.0	0.0	12.536021351589667	0.0
23	0.0	0.0	0.0	13.182809984563121	0.0
24	0.0	0.0	0.0	13.69225059277138	0.0
25	0.0	0.0	0.0	14.165758499147781	0.0
26	0.0	0.0	0.0	14.608770888837931	0.0
27	0.0	0.0	0.0	15.062657648819304	0.0
28	0.0	0.0	0.0	15.507797632696867	0.0
29	0.0	0.0	0.0	16.00990986092626	0.0
30	0.0	0.0	0.0	16.593816265693963	0.0
31	0.0	0.0	0.0	17.1110580525894	0.0
32	0.0	0.0	0.0	17.591185140882196	0.0
33	0.0	0.0	0.0	18.072730625299933	0.0
34	0.0	0.0	0.0	18.596827993465922	0.0
35	0.0	0.0	0.0	19.162058849255224	0.0
36	0.0	0.0	0.0	19.679773434858976	0.0
37	0.0	0.0	0.0	20.207653192691478	0.0
38	0.0	0.0	0.0	20.78919560391761	0.0
39	0.0	0.0	0.0	21.660327223986023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGATA	30	2.1622363E-6	45.000004	8
CGATCGA	65	0.0	45.000004	41
ACAACGA	20	7.028227E-4	45.0	13
ACCGGTA	20	7.028227E-4	45.0	41
ATTCCGA	20	7.028227E-4	45.0	12
CCGGTAT	20	7.028227E-4	45.0	42
CGATAAC	20	7.028227E-4	45.0	16
TACCGGT	25	3.8866114E-5	45.0	40
CGTTCAT	85	0.0	42.352943	17
CCGATGA	405	0.0	41.666668	18
TCTTGCG	90	0.0	40.000004	1
GTTAGGG	135	0.0	40.0	3
CGTTTGG	295	0.0	39.661015	2
TTTGGGC	940	0.0	39.255318	4
CGATGAA	430	0.0	39.244186	19
TACGGCT	380	0.0	39.078945	7
TTGGGAC	760	0.0	39.078945	5
CCGATCG	75	0.0	39.0	40
ACACGCG	145	0.0	38.793106	36
TGCTACG	35	6.23893E-6	38.571426	4
>>END_MODULE