##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754871
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88816499772809	31.0	30.0	31.0	25.0	34.0
2	29.77303009388359	31.0	30.0	33.0	25.0	34.0
3	29.876193415828666	31.0	30.0	33.0	25.0	34.0
4	33.85681262096438	35.0	33.0	37.0	28.0	37.0
5	34.411784265126094	35.0	35.0	37.0	32.0	37.0
6	33.653221543813444	35.0	33.0	37.0	28.0	37.0
7	34.347681921811805	35.0	35.0	37.0	32.0	37.0
8	34.10057612492731	35.0	35.0	37.0	30.0	37.0
9	35.739334270358775	37.0	35.0	39.0	30.0	39.0
10	35.2489458463764	37.0	34.0	39.0	30.0	39.0
11	35.42720014413059	37.0	35.0	39.0	30.0	39.0
12	35.481796227434884	37.0	35.0	39.0	30.0	39.0
13	35.448354752003986	37.0	35.0	39.0	30.0	39.0
14	36.22107485914812	38.0	35.0	40.0	30.0	41.0
15	36.38875913897871	38.0	35.0	40.0	30.0	41.0
16	36.633424783837235	38.0	35.0	40.0	31.0	41.0
17	36.25238881875181	38.0	35.0	40.0	30.0	41.0
18	36.41626582555165	38.0	35.0	40.0	30.0	41.0
19	36.393476501283004	38.0	35.0	40.0	30.0	41.0
20	36.27766466058439	38.0	34.0	40.0	30.0	41.0
21	36.13883299265702	38.0	34.0	40.0	30.0	41.0
22	36.300891145639454	38.0	35.0	40.0	30.0	41.0
23	36.244402023657024	38.0	34.0	40.0	30.0	41.0
24	36.17467620295388	38.0	34.0	40.0	30.0	41.0
25	35.84422768923432	38.0	34.0	40.0	29.0	41.0
26	35.896021969316614	38.0	34.0	40.0	29.0	41.0
27	35.829997443271765	38.0	34.0	40.0	29.0	41.0
28	35.70332281939563	38.0	34.0	40.0	29.0	41.0
29	35.83632832629681	38.0	34.0	40.0	29.0	41.0
30	35.42949325116477	38.0	34.0	40.0	27.0	41.0
31	35.63938209309935	38.0	34.0	40.0	29.0	41.0
32	35.55133658598621	38.0	34.0	40.0	27.0	41.0
33	35.550091340109766	38.0	34.0	40.0	27.0	41.0
34	35.46824689251541	38.0	34.0	40.0	27.0	41.0
35	35.45048624202016	38.0	34.0	40.0	27.0	41.0
36	35.348193267458946	38.0	34.0	40.0	27.0	41.0
37	35.49113954569721	38.0	34.0	40.0	28.0	41.0
38	35.29674606654647	38.0	34.0	40.0	27.0	41.0
39	35.19704161373268	38.0	34.0	40.0	27.0	41.0
40	35.272810851125556	38.0	34.0	40.0	27.0	41.0
41	35.06011225759103	37.0	33.0	40.0	26.0	41.0
42	35.16424793110346	38.0	34.0	40.0	27.0	41.0
43	35.1427594913568	38.0	34.0	40.0	27.0	41.0
44	35.17431455175785	38.0	34.0	40.0	27.0	41.0
45	35.1952876716684	38.0	34.0	40.0	27.0	41.0
46	35.12096901324862	37.0	34.0	40.0	27.0	41.0
47	34.975205035032474	37.0	34.0	40.0	26.0	41.0
48	35.017542070102046	37.0	34.0	40.0	27.0	41.0
49	35.03765014154737	37.0	34.0	40.0	27.0	41.0
50	34.805834374350056	37.0	34.0	40.0	26.0	41.0
51	33.81476172750046	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	7.0
11	10.0
12	8.0
13	17.0
14	16.0
15	35.0
16	76.0
17	178.0
18	337.0
19	695.0
20	1430.0
21	2710.0
22	4327.0
23	5850.0
24	7158.0
25	8156.0
26	9326.0
27	11341.0
28	13966.0
29	17918.0
30	23475.0
31	29708.0
32	38068.0
33	48921.0
34	66147.0
35	70901.0
36	85708.0
37	108917.0
38	131264.0
39	68195.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.295204081227123	4.432545428291721	49.93859878045388	16.333651710027276
2	18.90985347165277	10.965847144743938	49.93634674003903	20.187952643564262
3	20.8063364468896	10.841852448961479	48.54167135841753	19.81013974573139
4	18.80784928815652	4.952236872260293	50.79543392182241	25.44447991776078
5	23.335245359803196	5.770787326576329	46.28751137611592	24.606455937504553
6	21.00040934146364	12.303691624131806	49.23384260357068	17.46205643083388
7	80.0493064377887	2.3462286933793983	13.711614302311256	3.892850566520637
8	81.42808506354065	8.40143547705502	5.827353282878796	4.343126176525526
9	76.23209793461399	4.8785819033980635	7.568577942456393	11.32074221953155
10	41.97723849505412	28.372662348931144	14.92148989694928	14.728609259065456
11	31.670179408137283	25.237027253663207	24.346808924968638	18.74598441323087
12	25.358637436065234	22.030651594775797	28.224425100447625	24.386285868711344
13	25.939928809028295	22.015814622630884	31.630569991429	20.413686576911815
14	20.88025636168299	28.312519622558025	29.09662710582338	21.710596909935607
15	18.68451695720196	24.193802649724258	33.869230636757806	23.25244975631598
16	21.376765036675142	23.718092230328093	29.002173881365163	25.902968851631602
17	20.761030692661393	26.331386422315866	30.0079086360451	22.89967424897764
18	21.47148320706452	23.99535814728609	29.11424601024546	25.418912635403927
19	22.431249842688352	26.1569195266476	27.633728146928416	23.77810248373563
20	26.81835704378629	25.408844690019883	29.285798500670975	18.486999765522853
21	24.978837443748667	28.92229268312069	26.453129077683474	19.64574079544717
22	22.8882815739378	23.626685884078206	30.637685114410278	22.84734742757372
23	23.912032651936556	26.687076334896958	27.722087614969976	21.678803398196514
24	22.19756753140603	26.072666720539004	28.35835526864855	23.371410479406414
25	21.4884397466587	29.878085129777144	25.89237101438524	22.74110410917892
26	20.67147896792962	26.66786775488792	29.082717444437527	23.57793583274493
27	22.723882623653576	27.29035822014622	26.79318718032617	23.192571975874024
28	18.597217272885036	28.88082864489429	30.11627152188917	22.4056825603315
29	20.987824409733584	25.124160286989433	29.170547020616766	24.717468282660214
30	20.451706318033146	28.42896335930245	28.40233629322096	22.71699402944344
31	22.28486721572295	26.616600717208637	25.17728194618683	25.921250120881584
32	24.313028318745854	27.65015479466028	25.032091575911647	23.004725310682222
33	23.650663490848107	24.614801734336066	25.61589993522072	26.11863483959511
34	21.867312428216213	26.25349231855509	27.851248756410037	24.02794649681866
35	22.17094046532454	25.23927929407806	27.279098018071963	25.310682222525436
36	21.638796562591487	30.735450163007982	25.281803116029096	22.34395015837143
37	20.072568690544475	26.26872670959674	27.873636687592978	25.785067912265802
38	20.34228364846444	28.18812750787883	25.468987416393002	26.00060142726373
39	21.06200927045813	26.322908152518774	27.560205651031765	25.054876925991326
40	20.286247584024288	23.968333662307863	29.353227240150964	26.392191513516877
41	20.1896747921168	25.02056642790623	29.306861702198123	25.482897077778855
42	21.559312783243758	24.34667645200306	29.56730355252752	24.526707212225666
43	22.885897060557365	25.74400129293614	26.132147082084224	25.237954564422267
44	21.072739580670074	24.50100745690323	29.212011058843167	25.214241903583527
45	20.555167704150776	24.806092696632934	27.464427696917753	27.174311902298538
46	22.513648027278833	26.44783015906029	27.04780022017007	23.990721593490807
47	18.281004304046654	26.701913307041863	31.014968120380832	24.00211426853065
48	20.41222937429044	26.057034910600617	28.86188501081642	24.668850704292524
49	21.04439036603605	23.401216896661815	31.352509236677523	24.20188350062461
50	21.633762589899465	24.33117711503025	28.18415331891144	25.85090697615884
51	19.82749370422231	23.6461594100184	28.3962425368043	28.130104348954987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	383.0
2	639.0
3	1264.0
4	1889.0
5	1426.0
6	963.0
7	943.5
8	924.0
9	990.0
10	1056.0
11	1046.5
12	1037.0
13	1093.0
14	1149.0
15	1160.5
16	1172.0
17	1259.5
18	1347.0
19	1553.0
20	1759.0
21	2201.0
22	2643.0
23	2987.5
24	3332.0
25	4006.5
26	6414.5
27	8148.0
28	9129.0
29	10110.0
30	11771.0
31	13432.0
32	15406.5
33	17381.0
34	19934.0
35	22487.0
36	24178.0
37	25869.0
38	28339.5
39	30810.0
40	33879.5
41	36949.0
42	39653.5
43	42358.0
44	44983.0
45	47608.0
46	64488.5
47	81369.0
48	75315.5
49	69262.0
50	66069.5
51	62877.0
52	57420.0
53	51963.0
54	47806.0
55	43649.0
56	41334.5
57	39020.0
58	37029.0
59	35038.0
60	31757.5
61	28477.0
62	24673.0
63	20869.0
64	18072.5
65	15276.0
66	12854.0
67	10432.0
68	8647.0
69	6862.0
70	5743.0
71	4624.0
72	3962.5
73	3301.0
74	2616.0
75	1491.5
76	1052.0
77	791.5
78	531.0
79	374.0
80	217.0
81	191.0
82	165.0
83	119.0
84	73.0
85	40.5
86	8.0
87	6.5
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	754871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.69660746741417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33787060295887	22.81920433862456
2	8.814057871115917	5.41123349329435
3	2.934942126149613	2.7027829915797796
4	1.610375322458544	1.977322365948818
5	1.0100467322783957	1.5502504032247142
6	0.8288379246103733	1.5265507455522511
7	0.7046087153534947	1.5140368007327634
8	0.6594329738117224	1.619388411853444
9	0.615633548991226	1.7008075257439284
>10	8.318602168387693	49.123541207089474
>50	0.10995660183025227	2.2282323609248764
>100	0.04731200395133443	2.523940444522285
>500	0.003504592885284032	0.7397408698021786
>1k	0.003942666995944536	2.374002056987395
>5k	8.76148221321008E-4	2.188965984119226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	8969	1.1881500282829782	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	7309	0.9682449054209263	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	4633	0.6137472495300522	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACACCTTGT	3007	0.3983462074977049	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	2228	0.295149767311236	TruSeq Adapter, Index 20 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTA	1871	0.2478569185993368	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTC	1307	0.173142166012471	No Hit
GAACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCT	1248	0.16532626104327758	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCT	1212	0.1605572342824138	No Hit
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1132	0.14995939703604988	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	1016	0.13459253302882215	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	979	0.1296910333023788	No Hit
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	908	0.12028545274623081	TruSeq Adapter, Index 20 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	809	0.10717062915385543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14519037027518608	0.0
2	0.0	0.0	0.0	0.781060605057023	0.0
3	0.0	0.0	0.0	0.989175633982495	0.0
4	0.0	0.0	0.0	1.4854193630434869	0.0
5	0.0	0.0	0.0	2.689466147196011	0.0
6	0.0	0.0	0.0	3.0493951946756463	0.0
7	0.0	0.0	0.0	3.5323916271786837	0.0
8	0.0	0.0	0.0	4.204956873426055	0.0
9	0.0	0.0	0.0	4.43572477946563	0.0
10	0.0	0.0	0.0	5.960356140320664	0.0
11	0.0	0.0	0.0	6.783675621397563	0.0
12	0.0	0.0	0.0	9.006174564925663	0.0
13	0.0	0.0	0.0	9.364646409783923	0.0
14	0.0	0.0	0.0	9.529442778964883	0.0
15	0.0	0.0	0.0	9.978261186348396	0.0
16	0.0	0.0	0.0	10.418866269865978	0.0
17	0.0	0.0	0.0	10.963065212466766	0.0
18	0.0	0.0	0.0	11.507926519895452	0.0
19	0.0	0.0	0.0	12.41072978032008	0.0
20	0.0	0.0	0.0	12.892533956132903	0.0
21	0.0	0.0	0.0	13.476077369510817	0.0
22	0.0	0.0	0.0	14.133408224716542	0.0
23	0.0	0.0	0.0	14.736822582931389	0.0
24	0.0	0.0	0.0	15.230549325646368	0.0
25	0.0	0.0	0.0	15.669167314680257	0.0
26	0.0	0.0	0.0	16.06473158990079	0.0
27	0.0	0.0	0.0	16.489704863479986	0.0
28	0.0	0.0	0.0	16.91149878588527	0.0
29	0.0	0.0	0.0	17.360052247337624	0.0
30	0.0	0.0	0.0	17.880406056134095	0.0
31	0.0	0.0	0.0	18.350420138010335	0.0
32	0.0	0.0	0.0	18.79950349132501	0.0
33	0.0	0.0	0.0	19.274021654030953	0.0
34	0.0	0.0	0.0	19.737809506524957	0.0
35	0.0	0.0	0.0	20.240676883864925	0.0
36	0.0	0.0	0.0	20.73095932947484	0.0
37	0.0	0.0	0.0	21.222434031774966	0.0
38	0.0	0.0	0.0	21.71245153145372	0.0
39	0.0	0.0	0.0	22.205648382306382	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	35	1.2111923E-7	45.000004	45
TCGTACG	30	2.1645083E-6	45.000004	30
CCGATCG	25	3.889529E-5	45.0	40
ATAGCCG	20	7.0317474E-4	45.0	23
TACGGGA	90	0.0	45.0	4
CGTAACA	20	7.0317474E-4	45.0	30
CGATCGA	25	3.889529E-5	45.0	41
CGGGATA	185	0.0	41.351353	6
ACCGGTC	60	3.6379788E-12	41.250004	39
TACGGCT	1195	0.0	40.857742	7
CGATGAA	1375	0.0	40.745457	19
ATGAATG	1525	0.0	39.836067	21
CGGTCGA	85	0.0	39.705883	41
CGTTCAT	165	0.0	39.545452	17
CGTAAGC	40	3.4561162E-7	39.375	43
CCGCGTA	40	3.4561162E-7	39.375	40
TTGGGAC	1445	0.0	39.083046	5
TGGGTAC	265	0.0	39.056602	6
TTTGGGC	1590	0.0	38.915092	4
GCGAACC	35	6.2454383E-6	38.57143	33
>>END_MODULE