##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432229
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11300722533657	31.0	30.0	31.0	26.0	34.0
2	30.421491848071277	31.0	30.0	33.0	27.0	34.0
3	30.46988054943097	31.0	30.0	34.0	26.0	34.0
4	34.079779468753834	35.0	33.0	37.0	30.0	37.0
5	34.48397030277931	35.0	35.0	37.0	32.0	37.0
6	34.01607712578286	35.0	35.0	37.0	30.0	37.0
7	34.50326563002483	35.0	35.0	37.0	32.0	37.0
8	34.74089429445965	35.0	35.0	37.0	32.0	37.0
9	36.05271511166534	38.0	35.0	39.0	31.0	39.0
10	35.28400917106441	37.0	34.0	39.0	30.0	39.0
11	35.41958776481911	37.0	35.0	39.0	30.0	39.0
12	35.46576467566961	37.0	35.0	39.0	30.0	39.0
13	35.51234646448989	37.0	35.0	39.0	30.0	39.0
14	36.208072572640894	38.0	35.0	40.0	30.0	41.0
15	36.48985838525412	38.0	35.0	40.0	30.0	41.0
16	36.62634159207272	38.0	35.0	40.0	31.0	41.0
17	36.36687033956537	38.0	35.0	40.0	30.0	41.0
18	36.49580893461568	38.0	35.0	40.0	31.0	41.0
19	36.48754479685537	38.0	35.0	40.0	30.0	41.0
20	36.53045492088685	38.0	35.0	40.0	30.0	41.0
21	36.223284416362624	38.0	34.0	40.0	30.0	41.0
22	36.42792362381978	38.0	35.0	40.0	30.0	41.0
23	36.461149066814116	38.0	35.0	40.0	30.0	41.0
24	36.36712714787763	38.0	35.0	40.0	30.0	41.0
25	35.85222185461873	38.0	34.0	40.0	29.0	41.0
26	35.98379562685521	38.0	34.0	40.0	30.0	41.0
27	36.02619213426216	38.0	34.0	40.0	30.0	41.0
28	35.89822987351612	38.0	34.0	40.0	29.0	41.0
29	36.06811204245898	38.0	34.0	40.0	30.0	41.0
30	35.546811990865955	38.0	34.0	40.0	28.0	41.0
31	35.71658310756567	38.0	34.0	40.0	29.0	41.0
32	35.773853674788135	38.0	34.0	40.0	29.0	41.0
33	35.81177107505512	38.0	34.0	40.0	29.0	41.0
34	35.80798604443478	38.0	34.0	40.0	29.0	41.0
35	35.827103225373584	38.0	34.0	40.0	29.0	41.0
36	35.575544445189934	38.0	34.0	40.0	28.0	41.0
37	35.58671676356746	38.0	34.0	40.0	29.0	41.0
38	35.47346429786062	38.0	34.0	40.0	28.0	41.0
39	35.44778346663458	38.0	34.0	40.0	28.0	41.0
40	35.395815644022036	38.0	34.0	40.0	28.0	41.0
41	35.2689569649422	38.0	34.0	40.0	27.0	41.0
42	35.45079113155295	38.0	34.0	40.0	28.0	41.0
43	35.41569399554403	38.0	34.0	40.0	28.0	41.0
44	35.36064447318435	38.0	34.0	40.0	28.0	41.0
45	35.43588005432306	38.0	34.0	40.0	28.0	41.0
46	35.31320202947974	37.0	34.0	40.0	28.0	41.0
47	35.23172901401803	37.0	34.0	40.0	28.0	41.0
48	35.187632019138	37.0	34.0	40.0	28.0	41.0
49	35.160563034872716	37.0	34.0	40.0	28.0	41.0
50	34.98791381420497	37.0	34.0	40.0	27.0	41.0
51	33.88448484483919	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	8.0
12	5.0
13	9.0
14	14.0
15	27.0
16	55.0
17	84.0
18	165.0
19	329.0
20	587.0
21	946.0
22	1400.0
23	2088.0
24	2815.0
25	3836.0
26	4957.0
27	6232.0
28	8059.0
29	10229.0
30	13234.0
31	16544.0
32	21504.0
33	28319.0
34	38359.0
35	41585.0
36	50216.0
37	64990.0
38	77843.0
39	37783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.900524953207675	4.423349659555467	55.08931608013344	16.58680930710341
2	20.34130056058247	4.446022825863142	55.14738714894188	20.06528946461251
3	21.42729895495212	4.317155952053194	53.153536666905744	21.102008426088947
4	19.48828051796617	4.847661771884812	49.46521404163071	26.198843668518307
5	18.33727028959186	5.530633067193548	51.66312302043593	24.468973622778666
6	22.143817282042622	6.865804932107748	53.84853862188793	17.1418391639617
7	85.51462303547424	1.9015383049263237	8.743281917687153	3.8405567419122733
8	88.1863549183419	2.798053809438978	5.20117807921264	3.814413193006485
9	82.74317549262081	4.939048513635133	7.144823692996074	5.1729523007479825
10	40.793653364304575	28.020563173688025	16.477145217003024	14.708638245004385
11	30.65782258941441	27.5205967207198	22.932750926013757	18.888829763852033
12	26.176633219890384	24.11800226268945	28.70954979883349	20.995814718586676
13	24.67904744938447	25.78031552718582	30.035698668992595	19.504938354437115
14	21.2523916720072	28.420813966670444	29.57645137184224	20.750342989480114
15	19.95215499191401	27.135846970008952	33.119480645676255	19.79251739240079
16	22.13340613424828	25.450629180365038	30.680033038042332	21.735931647344348
17	22.313866029350184	26.331643642606117	28.232025153333073	23.122465174710626
18	22.909152324346586	25.611192215237754	28.843275208280794	22.636380252134863
19	24.274169479604563	27.831774360350646	26.992635848126806	20.901420311917988
20	26.52644778578022	27.121271363096877	27.634887987617674	18.717392863505225
21	25.784479986303555	26.981067906133095	27.299880387479785	19.934571720083564
22	24.986291988737452	25.76111274347626	26.402439447607634	22.850155820178657
23	22.979485411668353	27.921310231381973	25.88627787584822	23.212926481101455
24	23.193955056231765	26.656008736109793	27.6948099271451	22.455226280513337
25	22.193328073775707	28.676465484731473	26.190052032603088	22.940154408889732
26	22.7985627988867	28.899032688690486	27.01276406719586	21.28964044522695
27	23.296215663456177	28.059431458786893	27.941669809290907	20.702683068466023
28	21.003680919142397	28.232719229852695	29.535963574864248	21.227636276140657
29	22.135719722647025	26.76844913228867	28.180200773201243	22.915630371863063
30	23.934303343829313	27.535866404151506	27.680002961393154	20.84982729062603
31	24.055535375923412	28.360660668303144	25.57741382461612	22.00639013115733
32	24.07843990107096	30.542837245996914	24.71351991652573	20.665202936406395
33	24.433575720277908	28.495542871949826	25.458032663241013	21.612848744531256
34	23.061849158663577	28.31739656524666	26.32354608321052	22.297208192879236
35	23.788084557028796	28.19616453315256	25.564689088423037	22.4510618213956
36	25.07791008932765	27.981463529749277	25.7257148409755	21.214911539947572
37	22.84113282542356	29.927191373091578	27.575891483449745	19.655784318035117
38	22.04109395713846	30.24646657211802	26.048229063760182	21.664210406983337
39	23.327680465679073	29.173655631621198	26.247660383731773	21.251003518967952
40	24.349129743723815	27.915757619224994	26.549120952087897	21.185991684963295
41	22.01749535547129	28.446957515576234	26.216195581508877	23.319351547443603
42	22.485765647376738	28.621864798521152	26.33141228376624	22.560957270335862
43	22.61925969798417	28.019175020648778	27.37622880463828	21.98533647672877
44	22.027212426746008	28.018943661808905	27.05255778765423	22.90128612379086
45	23.045422681032505	27.402603712383943	25.486721159385418	24.06525244719813
46	24.05993119388102	28.11819660411495	26.01907784993603	21.802794352068
47	21.837960895728884	28.80949681765916	27.75866496695039	21.59387731966157
48	22.57391336536882	27.473862235065212	27.55414375250157	22.398080647064404
49	22.990127918302566	27.085873460596122	28.227397976535585	21.696600644565727
50	22.395535699825786	27.555069187861065	26.53014952721821	23.51924558509494
51	22.116748297777335	27.298260875600665	25.314127464839242	25.27086336178276
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	231.0
2	374.0
3	671.0
4	968.0
5	735.5
6	503.0
7	552.5
8	602.0
9	707.5
10	813.0
11	905.5
12	998.0
13	1045.5
14	1093.0
15	1127.0
16	1161.0
17	1116.5
18	1072.0
19	1198.0
20	1324.0
21	1515.0
22	1706.0
23	1861.0
24	2016.0
25	2351.5
26	3487.0
27	4287.0
28	5057.0
29	5827.0
30	6589.0
31	7351.0
32	8921.5
33	10492.0
34	11284.5
35	12077.0
36	13255.5
37	14434.0
38	16226.5
39	18019.0
40	20065.0
41	22111.0
42	24167.0
43	26223.0
44	28847.0
45	31471.0
46	35401.0
47	39331.0
48	41523.5
49	43716.0
50	42268.5
51	40821.0
52	35867.5
53	30914.0
54	28073.0
55	25232.0
56	22965.0
57	20698.0
58	19072.0
59	17446.0
60	16333.0
61	15220.0
62	12923.5
63	10627.0
64	9014.0
65	7401.0
66	5986.5
67	4572.0
68	3762.5
69	2953.0
70	2612.0
71	2271.0
72	1819.0
73	1367.0
74	1127.0
75	754.5
76	622.0
77	425.5
78	229.0
79	172.0
80	115.0
81	93.5
82	72.0
83	53.0
84	34.0
85	18.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.310521835717054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.27389651644711	28.085083596364058
2	9.076160508867286	6.772725697011313
3	3.196674155305613	3.5780874261960727
4	1.728481931257933	2.5796225135536597
5	1.181875231748495	2.2048190820622686
6	0.9643130840465877	2.158741462727272
7	0.7822628580490735	2.0430644812557275
8	0.7037191934459538	2.1004904266622764
9	0.6276587407406126	2.1076447636603164
>10	6.338424706917614	42.59372231027248
>50	0.08080577445255786	2.033308436110731
>100	0.04134248925479705	2.3806068655429775
>500	0.003758408114072459	1.0164205044420194
>1k	6.264013523454099E-4	0.34566243413882425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1479	0.3421797241739911	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	970	0.22441807467800634	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	750	0.173519129905675	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	746	0.17259369454617807	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	734	0.16981738846768726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	618	0.1429797630422762	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	531	0.1228515439732179	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	441	0.1020292483845369	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24871075286480082	0.0
2	0.0	0.0	0.0	1.0297781962802126	0.0
3	0.0	0.0	0.0	1.3143495693255196	0.0
4	0.0	0.0	0.0	1.7856275261493328	0.0
5	0.0	0.0	0.0	2.5653068165254993	0.0
6	0.0	0.0	0.0	2.983603599018113	0.0
7	0.0	0.0	0.0	3.426887136217144	0.0
8	0.0	0.0	0.0	4.129986650594939	0.0
9	0.0	0.0	0.0	4.413863947120624	0.0
10	0.0	0.0	0.0	5.166936970911253	0.0
11	0.0	0.0	0.0	6.368152067538272	0.0
12	0.0	0.0	0.0	7.661447982435237	0.0
13	0.0	0.0	0.0	8.022136413799167	0.0
14	0.0	0.0	0.0	8.145682034292006	0.0
15	0.0	0.0	0.0	8.352748195979446	0.0
16	0.0	0.0	0.0	8.754849859680863	0.0
17	0.0	0.0	0.0	9.472524980970736	0.0
18	0.0	0.0	0.0	10.206163862211929	0.0
19	0.0	0.0	0.0	10.807002769365313	0.0
20	0.0	0.0	0.0	11.37036154445907	0.0
21	0.0	0.0	0.0	12.144256863838383	0.0
22	0.0	0.0	0.0	12.968588410310275	0.0
23	0.0	0.0	0.0	13.803099745736635	0.0
24	0.0	0.0	0.0	14.467099616175684	0.0
25	0.0	0.0	0.0	15.06469949957083	0.0
26	0.0	0.0	0.0	15.58386873624861	0.0
27	0.0	0.0	0.0	16.10974737928274	0.0
28	0.0	0.0	0.0	16.648119399670083	0.0
29	0.0	0.0	0.0	17.23230047035252	0.0
30	0.0	0.0	0.0	17.871082227245278	0.0
31	0.0	0.0	0.0	18.4689134694803	0.0
32	0.0	0.0	0.0	19.06628199403557	0.0
33	0.0	0.0	0.0	19.637506969685052	0.0
34	0.0	0.0	0.0	20.250376536511894	0.0
35	0.0	0.0	0.0	20.89262867600277	0.0
36	0.0	0.0	0.0	21.526089179578417	0.0
37	0.0	0.0	0.0	22.134331569607777	0.0
38	0.0	0.0	0.0	22.748589289473866	0.0
39	0.0	0.0	0.0	23.39361773504323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGG	20	7.028395E-4	45.000004	4
GCGACTC	20	7.028395E-4	45.000004	31
GACCGGA	20	7.028395E-4	45.000004	28
ATCGTCG	20	7.028395E-4	45.000004	21
CATACTC	20	7.028395E-4	45.000004	30
GCGATGA	20	7.028395E-4	45.000004	9
GCGATAT	20	7.028395E-4	45.000004	9
TACCGAA	20	7.028395E-4	45.000004	30
CGAACGA	25	3.886753E-5	45.0	22
TCTAGCG	25	3.886753E-5	45.0	1
CGCTAGA	25	3.886753E-5	45.0	24
GTCGTTT	25	3.886753E-5	45.0	9
CGGGACG	35	1.2095734E-7	45.0	6
CTAGCGG	55	1.8189894E-12	45.0	2
TAGCGGG	150	0.0	40.5	3
TCGTCTT	50	1.0786607E-9	40.5	38
GGCGATA	45	1.9235813E-8	40.0	8
TGTTTCG	125	0.0	39.6	1
CGTTTGG	285	0.0	39.473682	2
TTTAGCG	40	3.4515506E-7	39.375004	1
>>END_MODULE