##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934063.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	696699
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95955498716088	31.0	30.0	31.0	26.0	34.0
2	30.11483725396477	31.0	30.0	33.0	26.0	34.0
3	30.19686550432827	31.0	30.0	34.0	25.0	34.0
4	33.89311165941102	35.0	33.0	37.0	28.0	37.0
5	34.35353000363141	35.0	35.0	37.0	32.0	37.0
6	33.86122557948268	35.0	35.0	37.0	29.0	37.0
7	34.25298873688637	35.0	35.0	37.0	31.0	37.0
8	34.32845604773367	35.0	35.0	37.0	31.0	37.0
9	35.77447362490832	37.0	35.0	39.0	30.0	39.0
10	35.24530536142581	37.0	34.0	39.0	30.0	39.0
11	35.30365480645157	37.0	34.0	39.0	30.0	39.0
12	35.186869795995115	37.0	34.0	39.0	30.0	39.0
13	35.1449894430737	37.0	34.0	39.0	29.0	39.0
14	35.83155853532157	38.0	34.0	40.0	27.0	41.0
15	36.08041779879116	38.0	34.0	40.0	29.0	41.0
16	36.22336618826782	38.0	35.0	40.0	30.0	41.0
17	35.916639754040126	38.0	34.0	40.0	29.0	41.0
18	36.037439410706774	38.0	34.0	40.0	30.0	41.0
19	35.97133625855642	38.0	34.0	40.0	30.0	41.0
20	35.89428863827851	38.0	34.0	40.0	29.0	41.0
21	35.67452084759702	38.0	34.0	40.0	29.0	41.0
22	35.87018640761649	38.0	34.0	40.0	29.0	41.0
23	35.888837216645925	38.0	34.0	40.0	29.0	41.0
24	35.82586884723532	38.0	34.0	40.0	29.0	41.0
25	35.44241343822799	37.0	34.0	40.0	28.0	41.0
26	35.51062654029933	37.0	34.0	40.0	29.0	41.0
27	35.48080304406925	37.0	34.0	40.0	28.0	41.0
28	35.340774136319986	37.0	34.0	40.0	27.0	41.0
29	35.42756628041665	37.0	34.0	40.0	28.0	41.0
30	34.95343326170986	37.0	33.0	40.0	26.0	41.0
31	35.0388403026271	37.0	33.0	40.0	27.0	41.0
32	34.94466333380699	37.0	33.0	40.0	26.0	41.0
33	34.884000120568565	37.0	33.0	40.0	26.0	41.0
34	34.71040434965459	37.0	33.0	40.0	25.0	41.0
35	34.63850242357173	37.0	33.0	40.0	24.0	41.0
36	34.390022089883864	37.0	33.0	40.0	24.0	41.0
37	34.406834228267876	37.0	33.0	40.0	24.0	41.0
38	34.31575615868546	37.0	33.0	40.0	24.0	41.0
39	34.19632438111724	37.0	33.0	40.0	24.0	41.0
40	34.18173271384055	36.0	33.0	40.0	23.0	41.0
41	34.091762726801676	36.0	33.0	40.0	23.0	41.0
42	34.1525235431657	36.0	33.0	40.0	24.0	41.0
43	34.16227667902494	36.0	33.0	40.0	24.0	41.0
44	34.13598842541758	36.0	33.0	40.0	24.0	41.0
45	34.08775525729189	36.0	33.0	40.0	23.0	41.0
46	33.99659106730453	36.0	33.0	40.0	23.0	41.0
47	33.920985963809336	36.0	33.0	40.0	23.0	41.0
48	33.88219446274503	35.0	33.0	39.0	23.0	41.0
49	33.85786401300992	35.0	33.0	39.0	24.0	41.0
50	33.705897381796156	35.0	33.0	39.0	23.0	41.0
51	32.66297497197498	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	15.0
11	19.0
12	19.0
13	17.0
14	39.0
15	71.0
16	160.0
17	276.0
18	586.0
19	1093.0
20	2134.0
21	3504.0
22	5351.0
23	6967.0
24	8525.0
25	10363.0
26	11915.0
27	13591.0
28	15969.0
29	19237.0
30	23500.0
31	29597.0
32	37070.0
33	48904.0
34	66460.0
35	64419.0
36	75327.0
37	92637.0
38	106603.0
39	52323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.594840813608172	4.438645670511942	49.86701574137468	20.099497774505203
2	20.40364633794508	7.130482460861865	51.701380366557146	20.764490834635904
3	21.334177313301726	7.023262556713875	51.14016239437691	20.502397735607484
4	19.221069644136133	4.755281692667852	49.626022141556106	26.397626521639907
5	20.908455444890837	5.495917175135891	49.353881661951576	24.241745718021697
6	21.65368401562224	8.82691090413507	51.37670643994034	18.142698640302342
7	82.1607322530964	2.286209683091263	11.83968973688781	3.71336832692454
8	83.81926771819681	5.3539620409961834	7.288226335906899	3.538543904900107
9	79.07015798788287	4.494767467729967	8.58103714803667	7.8540373963505035
10	46.78548411867966	23.91176103310038	15.44626876168905	13.85648608653091
11	35.940628592835644	22.020987542683425	23.62641542473866	18.41196843974227
12	29.494659817223795	19.504118708366168	28.619820037060478	22.381401437349556
13	28.35672219997445	19.742098094011904	30.73278417221784	21.1683955337958
14	21.693012333877327	23.03950486508521	32.34739823079981	22.920084570237652
15	20.936588110503962	21.1266271374008	34.70106889775929	23.235715854335947
16	26.362030087598804	21.063328639771264	29.193956069981443	23.38068520264849
17	25.913055709854614	23.687273844228283	28.682400864648866	21.717269581268237
18	26.30547768835609	21.154329200989235	29.54532732212907	22.994865788525605
19	26.062187544405834	24.09878584582438	26.468245253689183	23.370781356080602
20	28.713978346459516	25.52206907143544	27.173858438149047	18.590094143955998
21	27.579342011399472	26.77770457543358	26.69359364661066	18.949359766556288
22	25.621538139139	22.016250920411828	30.920526654983	21.441684285466177
23	26.617951224273323	22.7893250887399	29.30117597412943	21.291547712857344
24	26.332605615911607	23.66244246080445	28.072381329670343	21.932570593613598
25	25.93458581108915	25.373941974941832	25.31652837164974	23.37494384231928
26	23.131797232377252	22.95180558605653	28.553076723233417	25.363320458332794
27	24.20729755604644	22.805113829645226	29.28825791338871	23.69933070091962
28	21.645215509136655	26.36504430177164	27.9492291506088	24.040511038482904
29	24.640483192885306	23.860232324145723	28.512887200928954	22.98639728204002
30	25.867124827220938	24.45173597206254	28.17572581559612	21.505413385120402
31	26.697181996816415	22.40695049081454	27.399924501111673	23.495943011257374
32	29.8748814050257	23.07668017321684	25.73564767568204	21.31279074607542
33	29.345958584697264	22.593257633497394	25.875306265690064	22.18547751611528
34	24.967310129625563	22.961852966632648	28.90028548914237	23.17055141459942
35	25.49580234792931	25.678377606398172	27.606613472963215	21.219206572709304
36	24.94778950450625	29.331748717882473	24.68383046337084	21.036631314240438
37	24.329588531058608	27.216057436568736	25.84530765796994	22.60904637440272
38	24.522067636095358	26.577618167960626	25.75043167852975	23.149882517414262
39	24.50455648709127	26.22064908949202	25.727609771221143	23.54718465219557
40	25.658569913262397	23.782580425693162	25.614074370711027	24.944775290333414
41	21.929843447457223	24.896691397576284	25.61723211889209	27.556233036074403
42	22.594549439571466	24.99084970697532	26.935591984486845	25.479008868966368
43	22.87300541553813	24.849899310893225	27.210603144255984	25.066492129312657
44	24.35083156427668	22.505701888476946	27.92956499148126	25.213901555765116
45	24.369778053363074	22.47771275687205	26.962576378034132	26.18993281173075
46	24.38011250195565	25.13926387148539	26.699478540948103	23.78114508561086
47	21.47340530128506	25.859804592801193	29.34466677862319	23.32212332729055
48	22.19782144082308	24.615795343469703	29.43236605765187	23.754017158055344
49	24.52120643204598	23.143710555060363	29.319835395199362	23.0152476176943
50	23.037351854961756	24.351692768326064	27.76435734800825	24.84659802870393
51	22.047971936230713	23.193229787899796	28.274764281274983	26.48403399459451
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	387.0
2	630.0
3	3032.5
4	5435.0
5	3824.5
6	2214.0
7	2164.0
8	2114.0
9	2176.5
10	2239.0
11	2290.5
12	2342.0
13	2286.0
14	2230.0
15	2064.0
16	1898.0
17	1830.0
18	1762.0
19	1787.0
20	1812.0
21	1823.0
22	1834.0
23	2241.0
24	2648.0
25	2962.0
26	4294.0
27	5312.0
28	5859.0
29	6406.0
30	7697.5
31	8989.0
32	10851.0
33	12713.0
34	14561.0
35	16409.0
36	17025.5
37	17642.0
38	19392.5
39	21143.0
40	22277.0
41	23411.0
42	25735.0
43	28059.0
44	30733.0
45	33407.0
46	41962.5
47	50518.0
48	50880.0
49	51242.0
50	51651.5
51	52061.0
52	51497.0
53	50933.0
54	50289.5
55	49646.0
56	49146.0
57	48646.0
58	46654.0
59	44662.0
60	41967.5
61	39273.0
62	35591.5
63	31910.0
64	28557.0
65	25204.0
66	21066.0
67	16928.0
68	14224.0
69	11520.0
70	9641.5
71	7763.0
72	6519.5
73	5276.0
74	4394.0
75	2723.0
76	1934.0
77	1407.5
78	881.0
79	616.0
80	351.0
81	304.0
82	257.0
83	153.5
84	50.0
85	39.5
86	29.0
87	28.0
88	27.0
89	15.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	696699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.76426198396503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40590213962761	24.823734891833833
2	7.41545944871695	5.155859500133299
3	3.408608175575551	3.5549324284918056
4	2.4588885188485943	3.4192577863446503
5	2.0477116756248788	3.559359257952365
6	1.8025782424968297	3.7599181361253016
7	1.5976891916976728	3.8879739940389055
8	1.360441287748118	3.7835789872862664
9	1.22406507764674	3.829834714026039
>10	7.192824439834457	38.754716867654786
>50	0.058616840630247025	1.3663255907661513
>100	0.021353277525725377	1.579563783740602
>500	0.003768225445716243	0.8814815954969671
>1k	0.0020934585809534684	1.6434624661090251
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3115	0.4471084356371977	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGC	2564	0.36802119710233544	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTC	2382	0.34189800760443173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCG	2217	0.31821489624644217	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGCAAT	1013	0.14539995033723316	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	869	0.1247310531520786	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	847	0.1215733049710133	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	698	0.10018673774470754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09473244543195843	0.0
2	0.0	0.0	0.0	0.39313964854262745	0.0
3	0.0	0.0	0.0	0.5619356422213897	0.0
4	0.0	0.0	0.0	0.8729738380563199	0.0
5	0.0	0.0	0.0	1.6428902582033273	0.0
6	0.0	0.0	0.0	2.095452986153274	0.0
7	0.0	0.0	0.0	2.530648099107362	0.0
8	0.0	0.0	0.0	3.1274625053287	0.0
9	0.0	0.0	0.0	3.510841841311671	0.0
10	0.0	0.0	0.0	4.32912922223227	0.0
11	0.0	0.0	0.0	5.000007176700412	0.0
12	0.0	0.0	0.0	5.999290941999343	0.0
13	0.0	0.0	0.0	6.241002211859067	0.0
14	0.0	0.0	0.0	6.361714312780698	0.0
15	0.0	0.0	0.0	6.562518390294804	0.0
16	0.0	0.0	0.0	6.789015055282123	0.0
17	0.0	0.0	0.0	7.0737865276109195	0.0
18	0.0	0.0	0.0	7.36128514609609	0.0
19	0.0	0.0	0.0	7.777533769963786	0.0
20	0.0	0.0	0.0	8.063597048366654	0.0
21	0.0	0.0	0.0	8.417551912662427	0.0
22	0.0	0.0	0.0	8.812844571328508	0.0
23	0.0	0.0	0.0	9.181870506488455	0.0
24	0.0	0.0	0.0	9.49563584847976	0.0
25	0.0	0.0	0.0	9.768781066141907	0.0
26	1.4353400823024004E-4	0.0	0.0	10.025132804841116	0.0
27	1.4353400823024004E-4	0.0	0.0	10.30330171279132	0.0
28	1.4353400823024004E-4	0.0	0.0	10.567404287934961	0.0
29	1.4353400823024004E-4	0.0	0.0	10.868251569185546	0.0
30	1.4353400823024004E-4	0.0	0.0	11.244167136740543	0.0
31	1.4353400823024004E-4	0.0	0.0	11.53467996939855	0.0
32	1.4353400823024004E-4	0.0	0.0	11.855622011801366	0.0
33	1.4353400823024004E-4	0.0	0.0	12.173980442056038	0.0
34	1.4353400823024004E-4	0.0	0.0	12.522337480030831	0.0
35	1.4353400823024004E-4	0.0	0.0	12.895238833412995	0.0
36	1.4353400823024004E-4	0.0	0.0	13.237998045066808	0.0
37	1.4353400823024004E-4	0.0	0.0	13.59798133770825	0.0
38	1.4353400823024004E-4	0.0	0.0	14.042936763221993	0.0
39	1.4353400823024004E-4	0.0	0.0	14.693289354513212	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	50	2.1827873E-11	45.0	40
ACCGAAT	85	0.0	45.0	39
CCCGTTA	25	3.889218E-5	45.0	29
CCGAATA	20	7.031371E-4	45.0	40
CGGTCTA	25	3.889218E-5	45.0	31
CGATCGA	115	0.0	44.999996	41
CGTTTTT	2120	0.0	43.089622	1
CCGATCG	130	0.0	41.53846	40
TACGAAT	170	0.0	41.02941	12
GATGAAT	1240	0.0	40.82661	20
CCGATGA	1120	0.0	39.977676	18
CGAATAT	175	0.0	39.857143	14
ATGAATG	1270	0.0	39.68504	21
TGAATGA	1295	0.0	38.91892	22
CTCACGA	35	6.2447452E-6	38.571426	24
TTTCGCG	175	0.0	38.571426	1
TGGGCGA	905	0.0	38.53591	6
CGATGAA	1170	0.0	38.46154	19
TTTGGGA	2835	0.0	38.015873	4
TCGAATG	155	0.0	37.741936	44
>>END_MODULE