##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152580
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.774931183641367	31.0	30.0	31.0	25.0	34.0
2	30.032795910342116	31.0	30.0	33.0	26.0	34.0
3	30.08648577795255	31.0	30.0	33.0	25.0	34.0
4	33.879872853585006	35.0	33.0	37.0	28.0	37.0
5	34.31803643990038	35.0	35.0	37.0	32.0	37.0
6	33.87291912439376	35.0	35.0	37.0	30.0	37.0
7	34.38387075632455	35.0	35.0	37.0	32.0	37.0
8	34.49368200288373	35.0	35.0	37.0	32.0	37.0
9	35.783202254554986	37.0	35.0	39.0	30.0	39.0
10	35.189395726831826	37.0	34.0	39.0	30.0	39.0
11	35.3108795386027	37.0	34.0	39.0	30.0	39.0
12	35.31428103290077	37.0	34.0	39.0	30.0	39.0
13	35.18761960938524	37.0	34.0	39.0	30.0	39.0
14	35.73110499410146	38.0	34.0	40.0	27.0	41.0
15	36.061757766417614	38.0	34.0	40.0	30.0	41.0
16	36.24567440031459	38.0	34.0	40.0	30.0	41.0
17	36.05075370297549	38.0	34.0	40.0	30.0	41.0
18	36.19168960545288	38.0	35.0	40.0	30.0	41.0
19	36.165611482500985	38.0	34.0	40.0	30.0	41.0
20	36.21920959496658	38.0	34.0	40.0	30.0	41.0
21	35.876340280508586	38.0	34.0	40.0	29.0	41.0
22	36.05080613448683	38.0	34.0	40.0	30.0	41.0
23	36.17492462970245	38.0	34.0	40.0	30.0	41.0
24	36.03664307248656	38.0	34.0	40.0	30.0	41.0
25	35.58621051251802	38.0	34.0	40.0	29.0	41.0
26	35.67552103814393	38.0	34.0	40.0	29.0	41.0
27	35.68054135535457	38.0	34.0	40.0	29.0	41.0
28	35.61662078909425	38.0	34.0	40.0	29.0	41.0
29	35.802143138025954	38.0	34.0	40.0	29.0	41.0
30	35.33272381701403	38.0	34.0	40.0	27.0	41.0
31	35.35806134486827	37.0	34.0	40.0	28.0	41.0
32	35.42928955302136	38.0	34.0	40.0	29.0	41.0
33	35.40968672171975	38.0	34.0	40.0	28.0	41.0
34	35.42055315244462	38.0	34.0	40.0	28.0	41.0
35	35.43990038012846	38.0	34.0	40.0	28.0	41.0
36	35.24505833005637	37.0	34.0	40.0	27.0	41.0
37	35.2633438196356	37.0	34.0	40.0	27.0	41.0
38	35.17119543845851	37.0	34.0	40.0	27.0	41.0
39	35.120808756062395	37.0	33.0	40.0	27.0	41.0
40	35.041257045484336	37.0	33.0	40.0	27.0	41.0
41	34.874649364267924	37.0	33.0	40.0	26.0	41.0
42	35.13640713068554	37.0	33.0	40.0	27.0	41.0
43	35.142194258749505	37.0	33.0	40.0	27.0	41.0
44	35.07040896578844	37.0	33.0	40.0	27.0	41.0
45	35.184814523528644	37.0	34.0	40.0	28.0	41.0
46	35.0317669419321	37.0	33.0	40.0	27.0	41.0
47	34.96196749246297	37.0	33.0	40.0	27.0	41.0
48	34.94409490103552	37.0	33.0	40.0	27.0	41.0
49	35.00236597194914	37.0	33.0	40.0	27.0	41.0
50	34.85503342508848	37.0	33.0	40.0	27.0	41.0
51	33.79378686590641	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	6.0
13	6.0
14	7.0
15	8.0
16	18.0
17	36.0
18	61.0
19	107.0
20	208.0
21	290.0
22	537.0
23	773.0
24	1060.0
25	1492.0
26	2005.0
27	2378.0
28	3067.0
29	3983.0
30	5039.0
31	6570.0
32	8327.0
33	10941.0
34	14857.0
35	15741.0
36	18491.0
37	22251.0
38	24665.0
39	9652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.470572814261374	5.102241447109713	50.9581858697077	18.46899986892122
2	19.882684493380523	5.314589068029886	51.772840477126756	23.02988596146284
3	20.353912701533623	4.970507274872198	52.07563245510551	22.599947568488663
4	20.49547778214707	4.523528640713068	47.50032769694587	27.480665880193992
5	17.390221523135406	5.50203172106436	51.391401232140524	25.71634552365972
6	20.156639140123215	6.7263075108139985	54.280377506881635	18.83667584218115
7	79.99213527329925	2.375147463625639	12.236859352470834	5.395857910604273
8	80.47450517761175	3.536505439769301	8.485384716214444	7.5036046664045095
9	74.14471097129375	5.63048892384323	11.984532704155198	8.240267400707825
10	38.03250753702975	29.442259798138682	18.342508847817538	14.182723817014026
11	28.326124000524317	27.48001048630227	25.47974832874558	18.714117184427842
12	25.282474767335167	23.069209594966576	31.5926071569013	20.05570848079696
13	22.73626949796828	28.15834316424171	31.14300694717525	17.96238039061476
14	17.921745969327567	30.168436230174333	30.210381439244987	21.699436361253113
15	16.375671778739022	26.80036702057937	36.4838117708743	20.340149429807315
16	17.744789618560755	26.295058330056364	31.731550661947832	24.22860138943505
17	18.553545680954254	26.79971162668764	27.788701009306592	26.858041683051514
18	19.747673351684362	24.494035915585265	32.2224406868528	23.535850045877574
19	20.539389172892907	25.8834709660506	29.802071044697865	23.775068816358633
20	23.216017826713855	28.4277100537423	29.05164503866824	19.304627080875605
21	22.953860270022282	27.39612006816097	28.94350504653297	20.706514615283787
22	20.113383143269104	26.017826713855026	29.31445798925154	24.55433215362433
23	20.17236859352471	26.574911521824617	26.71975357189671	26.532966312753963
24	21.375671778739022	26.75186787259143	28.480141565080615	23.392318783588937
25	18.189802071044696	27.44593000393236	26.637829335430595	27.726438589592345
26	17.557346965526282	28.900249049678855	26.629964608729846	26.912439376065016
27	18.583693799973787	28.07707432166732	27.286013894350504	26.053217984008388
28	15.53152444619216	25.793682002883735	30.668501769563505	28.0062917813606
29	16.258356272119546	23.649888583038408	31.59129636911784	28.50045877572421
30	18.23830121903264	25.85201205924761	27.998427054659853	27.911259667059902
31	19.072617643203564	30.379473063311053	24.581858697076946	25.96605059640844
32	18.484073928430984	30.994232533752786	24.576615545943113	25.945077991873116
33	18.42115611482501	30.125835627211956	24.22139205662603	27.231616201337005
34	16.624066063704287	28.50570192685804	27.15952287324682	27.710709136190854
35	15.454843360859877	28.39756193472277	25.87101848210775	30.276576222309608
36	19.6624721457596	27.728404771267535	25.178267138550268	27.4308559444226
37	17.590116660112727	28.43098702320094	28.259273823567966	25.719622493118365
38	16.369117839821733	30.172368593524705	25.98374623148512	27.474767335168437
39	18.113120985712413	27.557346965526282	29.46847555380784	24.861056494953466
40	19.484205007209333	27.47804430462708	27.04024118495216	25.99750950321143
41	16.236072879800762	29.787652379079827	25.179577926333724	28.796696814785687
42	18.69576615545943	28.252719884650674	25.22480010486302	27.82671385502687
43	20.20775986367807	25.909031327828025	26.54804037226373	27.335168436230173
44	17.755931314720147	25.774675580023594	27.416437278804562	29.052955826451697
45	18.470966050596406	24.825009830908375	25.517761174465853	31.186262944029362
46	20.58657753309739	26.417616987809673	26.99501900642286	26.00078647267007
47	15.583955957530476	27.90798269760126	29.38917289290864	27.118888451959627
48	16.270808756062394	26.214444881373705	28.966443832743476	28.548302529820425
49	18.564687377113646	23.82618953991349	31.751867872591426	25.857255210381442
50	18.978896316686328	24.07589461266221	28.34775199895137	28.597457071700095
51	16.686328483418535	25.11469393105256	25.303447371870497	32.89553021365841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	82.5
2	125.0
3	213.5
4	302.0
5	224.0
6	146.0
7	205.0
8	264.0
9	342.0
10	420.0
11	497.5
12	575.0
13	643.5
14	712.0
15	655.0
16	598.0
17	672.5
18	747.0
19	641.0
20	535.0
21	567.0
22	599.0
23	593.5
24	588.0
25	729.0
26	1024.5
27	1179.0
28	1367.0
29	1555.0
30	1835.5
31	2116.0
32	2358.0
33	2600.0
34	3034.5
35	3469.0
36	3870.5
37	4272.0
38	4786.5
39	5301.0
40	6302.0
41	7303.0
42	8385.5
43	9468.0
44	10682.0
45	11896.0
46	14014.0
47	16132.0
48	18018.0
49	19904.0
50	19417.5
51	18931.0
52	15072.0
53	11213.0
54	9924.0
55	8635.0
56	7543.5
57	6452.0
58	5511.0
59	4570.0
60	3991.5
61	3413.0
62	3001.0
63	2589.0
64	2238.0
65	1887.0
66	1486.5
67	1086.0
68	947.0
69	808.0
70	640.0
71	472.0
72	446.5
73	421.0
74	308.5
75	154.0
76	112.0
77	82.5
78	53.0
79	36.0
80	19.0
81	12.0
82	5.0
83	3.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.74125049154542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12763640531867	34.82173286144973
2	10.018340210912426	9.165028181937345
3	3.5304906006419072	4.844671647660244
4	1.8755731315910134	3.431642417092673
5	1.3597546996790464	3.109844016253769
6	1.0302040348464008	2.8273692489186
7	0.8797569922054103	2.8168829466509373
8	0.7866231086657497	2.8784899724734565
9	0.6318775790921596	2.6012583562721194
>10	3.6436840898670337	25.85135666535588
>50	0.07450710683172856	2.308952680561017
>100	0.035820724438331036	2.6844933805216935
>500	0.002865657955066483	1.182985974570717
>1k	0.002865657955066483	1.4752916502818192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1191	0.7805741250491546	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1060	0.6947175252326648	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	999	0.6547384978372002	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	806	0.5282474767335168	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	392	0.2569144055577402	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	315	0.20644907589461264	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	263	0.17236859352470835	No Hit
TGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	240	0.15729453401494298	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	213	0.13959889893826188	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	211	0.13828811115480405	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	205	0.13435574780443046	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.11993708218639401	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	171	0.11207235548564688	No Hit
TTTTTTGGGATAGCAATAATAGCTCTACTAAACCTATTCTTTTATACTCGC	158	0.10355223489317081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5957530475815965	0.0
2	0.0	0.0	0.0	3.0541355354568096	0.0
3	0.0	0.0	0.0	3.946782015991611	0.0
4	0.0	0.0	0.0	5.353257307641893	0.0
5	0.0	0.0	0.0	7.57635338838642	0.0
6	0.0	0.0	0.0	8.65382094638878	0.0
7	0.0	0.0	0.0	9.691309476995674	0.0
8	0.0	0.0	0.0	11.271464149954122	0.0
9	0.0	0.0	0.0	11.871149560886092	0.0
10	0.0	0.0	0.0	13.225848735089789	0.0
11	0.0	0.0	0.0	15.653427710053743	0.0
12	0.0	0.0	0.0	18.191768252719886	0.0
13	0.0	0.0	0.0	18.982173286144974	0.0
14	0.0	0.0	0.0	19.231878358893695	0.0
15	0.0	0.0	0.0	19.685410931970114	0.0
16	0.0	0.0	0.0	20.62590116660113	0.0
17	0.0	0.0	0.0	21.991086643072485	0.0
18	0.0	0.0	0.0	23.643990038012845	0.0
19	0.0	0.0	0.0	24.649364267925023	0.0
20	0.0	0.0	0.0	25.6547384978372	0.0
21	0.0	0.0	0.0	26.957006160702583	0.0
22	0.0	0.0	0.0	28.211430069471753	0.0
23	0.0	0.0	0.0	29.352470834971818	0.0
24	0.0	0.0	0.0	30.395202516712544	0.0
25	0.0	0.0	0.0	31.250491545418797	0.0
26	0.0	0.0	0.0	32.06186918337921	0.0
27	0.0	0.0	0.0	32.76641761698781	0.0
28	0.0	0.0	0.0	33.51684362301744	0.0
29	0.0	0.0	0.0	34.315768777035	0.0
30	0.0	0.0	0.0	35.247083497181805	0.0
31	0.0	0.0	0.0	36.05911652903395	0.0
32	0.0	0.0	0.0	36.81347489841394	0.0
33	0.0	0.0	0.0	37.52785424039848	0.0
34	0.0	0.0	0.0	38.33529951500852	0.0
35	0.0	0.0	0.0	39.14012321405165	0.0
36	0.0	0.0	0.0	39.85319176825272	0.0
37	0.0	0.0	0.0	40.555774020186135	0.0
38	0.0	0.0	0.0	41.28522742168043	0.0
39	0.0	0.0	0.0	42.078909424564166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCTTG	55	1.8189894E-12	45.000004	1
TCGTTTG	45	3.8016879E-10	45.000004	1
CTTGGCG	45	3.8016879E-10	45.000004	1
CAGGTTT	20	7.0140423E-4	45.0	13
CAGCGGA	25	3.874863E-5	45.0	37
GATACTC	20	7.0140423E-4	45.0	9
AGTCATC	20	7.0140423E-4	45.0	30
GGTCGTT	25	3.874863E-5	45.0	8
AAGAGTG	25	3.874863E-5	45.0	43
TGCCAGT	25	3.874863E-5	45.0	24
CCCTAGT	20	7.0140423E-4	45.0	26
TAGGTTG	20	7.0140423E-4	45.0	14
CGCATTA	20	7.0140423E-4	45.0	15
GACAATA	35	1.2026794E-7	45.0	9
TCGGGTT	20	7.0140423E-4	45.0	5
ATTAACT	20	7.0140423E-4	45.0	15
TCAGGGC	20	7.0140423E-4	45.0	4
ATCGTGT	25	3.874863E-5	45.0	10
TAGTTGG	20	7.0140423E-4	45.0	2
TTCCCTA	25	3.874863E-5	45.0	24
>>END_MODULE