SRR2934061.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551028
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	3233	0.5867215459105526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	3032	0.5502442707085665	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	2109	0.3827391711491975	Illumina PCR Primer Index 11 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1550	0.2812924207118332	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	1346	0.24427070856653382	Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	1296	0.2351967595113134	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1268	0.23011534804038997	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.15516452884426926	No Hit
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	837	0.1518979071843899	Illumina PCR Primer Index 11 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	834	0.15135347024107668	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	712	0.12921303454633884	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	710	0.12885007658413003	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	675	0.12249831224547574	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	563	0.10217266636178197	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	35	1.2103919E-7	45.000004	22
CCGATCG	20	7.030085E-4	45.000004	40
TAACGGA	20	7.030085E-4	45.000004	25
CGATCGA	20	7.030085E-4	45.000004	41
TATCGCG	20	7.030085E-4	45.000004	1
TACCCGC	25	3.8881535E-5	45.0	28
TCCGTTG	25	3.8881535E-5	45.0	1
ACGAACG	25	3.8881535E-5	45.0	21
TTGCGTC	30	2.1634369E-6	44.999996	1
ACGAGAC	30	2.1634369E-6	44.999996	25
CACCCGT	100	0.0	42.75	38
TCACGGG	85	0.0	42.352943	3
CGATGAA	590	0.0	41.5678	19
CCCGTTA	110	0.0	40.909092	40
GATGAAT	655	0.0	40.53435	20
CGCTCGA	50	1.0786607E-9	40.5	41
GCTCGAA	50	1.0786607E-9	40.5	42
AGAGACG	50	1.0786607E-9	40.5	33
GGCCGAT	225	0.0	40.0	8
TTTCGCG	170	0.0	39.705883	1