##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551028
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.943440260749	31.0	30.0	31.0	26.0	34.0
2	30.06931589683283	31.0	30.0	33.0	25.0	34.0
3	30.12356177907475	31.0	30.0	34.0	25.0	34.0
4	33.94310452463396	35.0	33.0	37.0	28.0	37.0
5	34.38973700066058	35.0	35.0	37.0	32.0	37.0
6	33.8423982084395	35.0	35.0	37.0	29.0	37.0
7	34.41557597799023	35.0	35.0	37.0	32.0	37.0
8	34.41439636461305	35.0	35.0	37.0	31.0	37.0
9	35.85561169305371	37.0	35.0	39.0	31.0	39.0
10	35.263322372002875	37.0	34.0	39.0	30.0	39.0
11	35.38304224104764	37.0	35.0	39.0	30.0	39.0
12	35.42979485615976	37.0	35.0	39.0	30.0	39.0
13	35.4111170394245	37.0	35.0	39.0	30.0	39.0
14	36.01394484490806	38.0	34.0	40.0	28.0	41.0
15	36.278666419855256	38.0	35.0	40.0	30.0	41.0
16	36.4768469115907	38.0	35.0	40.0	31.0	41.0
17	36.192438496773306	38.0	34.0	40.0	30.0	41.0
18	36.35188592957164	38.0	35.0	40.0	30.0	41.0
19	36.322067118186375	38.0	35.0	40.0	30.0	41.0
20	36.27784613486066	38.0	34.0	40.0	30.0	41.0
21	36.03052657941157	38.0	34.0	40.0	29.0	41.0
22	36.23747613551399	38.0	34.0	40.0	30.0	41.0
23	36.265726968502506	38.0	34.0	40.0	30.0	41.0
24	36.17286054429176	38.0	34.0	40.0	30.0	41.0
25	35.75678368431368	38.0	34.0	40.0	29.0	41.0
26	35.8701862700262	38.0	34.0	40.0	29.0	41.0
27	35.827830890626245	38.0	34.0	40.0	29.0	41.0
28	35.71343198530746	38.0	34.0	40.0	29.0	41.0
29	35.835523784635264	38.0	34.0	40.0	29.0	41.0
30	35.41119689017618	38.0	34.0	40.0	27.0	41.0
31	35.56935763699848	38.0	34.0	40.0	29.0	41.0
32	35.56089164252996	38.0	34.0	40.0	28.0	41.0
33	35.55606611642239	38.0	34.0	40.0	28.0	41.0
34	35.49962615329893	38.0	34.0	40.0	28.0	41.0
35	35.471796351546566	38.0	34.0	40.0	28.0	41.0
36	35.24118375109795	38.0	34.0	40.0	27.0	41.0
37	35.278806158670704	38.0	34.0	40.0	27.0	41.0
38	35.225084750684175	38.0	34.0	40.0	27.0	41.0
39	35.199271180411884	38.0	34.0	40.0	27.0	41.0
40	35.134559405329675	37.0	33.0	40.0	27.0	41.0
41	34.93410498196099	37.0	33.0	40.0	26.0	41.0
42	35.16304616099363	37.0	34.0	40.0	27.0	41.0
43	35.09819101751635	37.0	34.0	40.0	27.0	41.0
44	35.07877458132799	37.0	34.0	40.0	27.0	41.0
45	35.12520597864356	37.0	34.0	40.0	27.0	41.0
46	35.01581044883382	37.0	34.0	40.0	27.0	41.0
47	34.865210116364324	37.0	33.0	40.0	26.0	41.0
48	34.85372612644004	37.0	33.0	40.0	26.0	41.0
49	34.89207989430664	37.0	33.0	40.0	27.0	41.0
50	34.673096466967195	37.0	33.0	40.0	26.0	41.0
51	33.54884143818463	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	11.0
12	14.0
13	21.0
14	26.0
15	43.0
16	89.0
17	167.0
18	334.0
19	529.0
20	1096.0
21	1873.0
22	2725.0
23	3539.0
24	4469.0
25	5718.0
26	7020.0
27	8571.0
28	10954.0
29	13894.0
30	17530.0
31	22321.0
32	28583.0
33	36788.0
34	48462.0
35	52192.0
36	62913.0
37	79490.0
38	94893.0
39	46752.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.312455991347083	4.351503008921507	52.678085324157756	16.657955675573653
2	20.07484193180746	7.511596506892572	51.75236104154417	20.661200519755802
3	21.368061151157473	7.465682324673156	50.87926566345086	20.28699086071851
4	19.86323743983972	4.685787292115827	49.24776962332223	26.203205644722228
5	20.895671363342696	5.607881995107326	48.52548327852668	24.970963363023294
6	21.642275891606232	8.896825569662521	51.47125009981344	17.989648438917804
7	81.84647604114491	2.0812009553053565	11.804844762879563	4.267478240670165
8	83.80427128929927	5.257990519538027	6.546491285379327	4.391246905783372
9	78.61233911888324	4.995934870823262	8.34059249257751	8.051133517715977
10	39.97945657933898	30.984632359880077	15.227901304470917	13.808009756310025
11	29.148428029065677	23.43329195612564	28.15900462408444	19.259275390724245
12	24.998548168151164	21.319969221164804	30.91077041457058	22.770712196113447
13	24.961526456005863	22.7070130737458	32.83553648816394	19.495923982084395
14	20.466110615068565	26.858889203452456	31.869160913782967	20.805839267696015
15	17.99146322872885	23.971014177138002	36.13682063343423	21.900701960698914
16	22.393598873378483	24.055038945389345	30.79734605138033	22.75401612985184
17	21.939538462655257	25.482189652794414	29.534252342893648	23.044019541656684
18	22.63460296028514	23.201361818274208	30.74417270991674	23.419862511523913
19	22.920613834505687	24.966063430533474	29.0591403703623	23.05418236459853
20	25.528648271957145	26.758349847920616	29.52082289829192	18.192178981830324
21	24.118012151832573	27.747773252901847	29.367291680277592	18.766922914987987
22	23.052186095806384	22.58632955131137	31.919249112567783	22.442235240314467
23	22.139891257794524	25.759489535921947	29.882510507633004	22.21810869865052
24	21.783829496867675	26.718424472077647	29.32464411971805	22.17310191133663
25	23.050189827014236	28.00964742263551	26.55400451519705	22.386158235153207
26	21.664416327300973	26.103936642058116	27.86301240590315	24.368634624737762
27	22.078188404219024	26.108473616585727	29.943305966302987	21.870032012892267
28	19.33640395769362	27.273387196294923	30.90605196106187	22.484156884949584
29	21.193659850316138	25.70431992566621	30.007912483576156	23.0941077404415
30	22.041892607998143	25.703412530760687	31.244147302859382	21.010547558381788
31	23.09610400923365	26.544023171236308	27.733254934413498	22.626617885116545
32	24.596027788061587	26.645651400654774	27.23436921535748	21.52395159592616
33	23.202450692160834	24.96334124581691	27.183192142686035	24.65101591933622
34	22.182901776316267	25.94586844951618	28.70852297886859	23.162706795298966
35	23.037849256299136	26.102666289190385	27.755032412146026	23.104452042364453
36	23.449988022387245	26.8195082645528	26.872681606016393	22.857822107043564
37	20.36430090666899	27.53453545010417	29.81100778907787	22.29015585414897
38	21.246288754836414	27.447607018155157	27.307686723723656	23.99841750328477
39	20.440522078732844	27.55395370108234	27.141996413975335	24.863527806209483
40	23.09773732006359	24.876231334886796	27.816190828778208	24.209840516271406
41	20.695863005146744	25.611221208359648	27.277016775917012	26.415899010576595
42	22.80864130316427	24.721792721966942	28.738648489731922	23.73091748513687
43	22.020115130265612	24.343590525345356	28.48185573146918	25.15443861291985
44	22.144609711303236	24.68876354740594	29.132094920766278	24.034531820524546
45	21.85170263580072	23.874285880209353	27.46738822709554	26.806623256894387
46	22.695761376917325	25.56113300957483	27.782435738292794	23.96066987521505
47	18.899039613231995	27.343438083001224	30.700073317508362	23.05744898625841
48	20.288442692567347	27.647233897370004	28.807973460513804	23.256349949548845
49	22.496860413626894	24.186792685671147	30.025334465762178	23.291012434939784
50	21.25627009879716	25.136290714809412	28.00311417931575	25.604325007077684
51	20.57009807124139	25.00108887388663	27.176658899366274	27.25215415550571
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	551.0
1	656.5
2	762.0
3	1314.0
4	1866.0
5	1491.5
6	1117.0
7	1038.5
8	960.0
9	1035.5
10	1111.0
11	1171.5
12	1232.0
13	1253.5
14	1275.0
15	1192.0
16	1109.0
17	1125.5
18	1142.0
19	1272.5
20	1403.0
21	1691.0
22	1979.0
23	2192.5
24	2406.0
25	2955.5
26	4734.5
27	5964.0
28	6847.5
29	7731.0
30	9017.5
31	10304.0
32	11704.5
33	13105.0
34	14854.5
35	16604.0
36	17779.0
37	18954.0
38	20936.5
39	22919.0
40	25048.0
41	27177.0
42	29444.0
43	31711.0
44	33344.5
45	34978.0
46	44695.5
47	54413.0
48	53878.0
49	53343.0
50	51346.0
51	49349.0
52	43928.5
53	38508.0
54	35476.5
55	32445.0
56	29608.0
57	26771.0
58	24851.0
59	22931.0
60	20437.0
61	17943.0
62	15677.5
63	13412.0
64	12261.0
65	11110.0
66	9300.5
67	7491.0
68	6123.5
69	4756.0
70	3947.0
71	3138.0
72	2783.0
73	2428.0
74	1960.0
75	1190.5
76	889.0
77	616.5
78	344.0
79	282.5
80	221.0
81	178.0
82	135.0
83	82.0
84	29.0
85	21.5
86	14.0
87	7.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.986006509877605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54194004338096	26.079247996166327
2	9.078867696055653	6.352666486330411
3	3.167674551630781	3.324728474535844
4	1.7213870999291188	2.4089784113655788
5	1.1491508187547377	2.0102099012892216
6	0.9167369628001871	1.924377920902366
7	0.8680934686913938	2.1259786622773533
8	0.7458627005808932	2.087580583839842
9	0.7263468461454372	2.2870777938906
>10	6.929613944737939	42.473135308385324
>50	0.09501014278916195	2.3554668912231693
>100	0.05091703784833098	2.8874946148993854
>500	0.004724261243659576	1.1424730648915016
>1k	0.0036744254117352256	2.540583890003059
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	3233	0.5867215459105526	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	3032	0.5502442707085665	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	2109	0.3827391711491975	Illumina PCR Primer Index 11 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1550	0.2812924207118332	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	1346	0.24427070856653382	Illumina PCR Primer Index 11 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	1296	0.2351967595113134	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	1268	0.23011534804038997	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.15516452884426926	No Hit
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	837	0.1518979071843899	Illumina PCR Primer Index 11 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	834	0.15135347024107668	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	712	0.12921303454633884	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	710	0.12885007658413003	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	675	0.12249831224547574	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	563	0.10217266636178197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27403326146765683	0.0
2	0.0	0.0	0.0	1.0309820916541446	0.0
3	0.0	0.0	0.0	1.3636330640185255	0.0
4	0.0	0.0	0.0	1.9538027105700617	0.0
5	0.0	0.0	0.0	3.1795117489492366	0.0
6	0.0	0.0	0.0	3.8165029726257105	0.0
7	0.0	0.0	0.0	4.415383610270259	0.0
8	0.0	0.0	0.0	5.2258687398825465	0.0
9	0.0	0.0	0.0	5.532386738967893	0.0
10	0.0	0.0	0.0	6.723070333993917	0.0
11	0.0	0.0	0.0	7.861669461442975	0.0
12	0.0	0.0	0.0	9.537990809904397	0.0
13	0.0	0.0	0.0	9.933433509730904	0.0
14	0.0	0.0	0.0	10.09259057615947	0.0
15	0.0	0.0	0.0	10.446111631350856	0.0
16	0.0	0.0	0.0	10.896723941433104	0.0
17	0.0	0.0	0.0	11.54079284537265	0.0
18	0.0	0.0	0.0	12.222609377381911	0.0
19	0.0	0.0	0.0	13.00678005473406	0.0
20	0.0	0.0	0.0	13.551942913971704	0.0
21	0.0	0.0	0.0	14.233759445980967	0.0
22	0.0	0.0	0.0	14.984900948772113	0.0
23	0.0	0.0	0.0	15.716442721603984	0.0
24	0.0	0.0	0.0	16.294090318459315	0.0
25	0.0	0.0	0.0	16.830360707622845	0.0
26	0.0	0.0	0.0	17.282969286497238	0.0
27	0.0	0.0	0.0	17.79927698773928	0.0
28	1.814789811044085E-4	0.0	0.0	18.299796017625237	0.0
29	1.814789811044085E-4	0.0	0.0	18.86582895968989	0.0
30	1.814789811044085E-4	0.0	0.0	19.50300166234747	0.0
31	1.814789811044085E-4	0.0	0.0	20.031831413285712	0.0
32	1.814789811044085E-4	0.0	0.0	20.540698476302474	0.0
33	1.814789811044085E-4	0.0	0.0	21.05845800939335	0.0
34	1.814789811044085E-4	0.0	0.0	21.606343053347562	0.0
35	1.814789811044085E-4	0.0	0.0	22.199779321558978	0.0
36	1.814789811044085E-4	0.0	0.0	22.742945912004473	0.0
37	1.814789811044085E-4	0.0	0.0	23.310793643880167	0.0
38	1.814789811044085E-4	0.0	0.0	23.915118650957847	0.0
39	1.814789811044085E-4	0.0	0.0	24.687130236576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	35	1.2103919E-7	45.000004	22
CCGATCG	20	7.030085E-4	45.000004	40
TAACGGA	20	7.030085E-4	45.000004	25
CGATCGA	20	7.030085E-4	45.000004	41
TATCGCG	20	7.030085E-4	45.000004	1
TACCCGC	25	3.8881535E-5	45.0	28
TCCGTTG	25	3.8881535E-5	45.0	1
ACGAACG	25	3.8881535E-5	45.0	21
TTGCGTC	30	2.1634369E-6	44.999996	1
ACGAGAC	30	2.1634369E-6	44.999996	25
CACCCGT	100	0.0	42.75	38
TCACGGG	85	0.0	42.352943	3
CGATGAA	590	0.0	41.5678	19
CCCGTTA	110	0.0	40.909092	40
GATGAAT	655	0.0	40.53435	20
CGCTCGA	50	1.0786607E-9	40.5	41
GCTCGAA	50	1.0786607E-9	40.5	42
AGAGACG	50	1.0786607E-9	40.5	33
GGCCGAT	225	0.0	40.0	8
TTTCGCG	170	0.0	39.705883	1
>>END_MODULE