FastQCFastQC Report
Sat 14 Jan 2017
SRR2934060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672184
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC14340.2133344441402949TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.16662104423788723No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT10350.1539756971305476TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC8090.12035395070397392TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC7910.11767611249300786TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC253.8890703E-545.00000411
TCGTTAG207.0311944E-445.01
ATGATCG207.0311944E-445.027
TTGTTCG950.042.631581
ACACGCG1550.040.64516436
CGTTTTT6800.040.3676451
TTTCGCG2900.040.3448261
GTTCGCC451.9263098E-840.028
TACGAAT900.040.012
TTGGGCA9850.039.289345
GACCGAT3850.039.1558469
TTTACGG750.039.02
CGTTTGG5150.038.8834952
CTGTTCG1100.038.8636361
GATAACG356.244418E-638.57142617
CGTAGGG1400.038.5714263
TTACGGG1400.038.5714263
ACGAACG356.244418E-638.57142621
TTTGGGC16600.038.222894
TTGGGTA6550.038.1297725
Produced by FastQC (version 0.11.5)