##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934060.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672184
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.112980969496448	31.0	30.0	31.0	26.0	34.0
2	30.512489139878365	31.0	30.0	33.0	27.0	34.0
3	30.52138997655404	31.0	30.0	34.0	27.0	34.0
4	34.079476750413576	35.0	33.0	37.0	30.0	37.0
5	34.44096259357557	35.0	35.0	37.0	32.0	37.0
6	34.01265427323471	35.0	35.0	37.0	30.0	37.0
7	34.420762767337514	35.0	35.0	37.0	32.0	37.0
8	34.71312170477131	35.0	35.0	37.0	32.0	37.0
9	35.98329326493936	37.0	35.0	39.0	31.0	39.0
10	35.30735185603942	37.0	34.0	39.0	30.0	39.0
11	35.43114385346869	37.0	35.0	39.0	30.0	39.0
12	35.46178873641741	37.0	35.0	39.0	30.0	39.0
13	35.527574295133476	37.0	35.0	39.0	30.0	39.0
14	36.23291539221404	38.0	35.0	40.0	30.0	41.0
15	36.49992859098104	38.0	35.0	40.0	30.0	41.0
16	36.58614456755888	38.0	35.0	40.0	31.0	41.0
17	36.36072414695976	38.0	35.0	40.0	30.0	41.0
18	36.45668150387394	38.0	35.0	40.0	30.0	41.0
19	36.45149096080835	38.0	35.0	40.0	30.0	41.0
20	36.495691655856135	38.0	35.0	40.0	30.0	41.0
21	36.151596884186475	38.0	34.0	40.0	30.0	41.0
22	36.33280917129834	38.0	35.0	40.0	30.0	41.0
23	36.4165288075884	38.0	35.0	40.0	30.0	41.0
24	36.34214143746355	38.0	35.0	40.0	30.0	41.0
25	35.84346547969008	38.0	34.0	40.0	29.0	41.0
26	35.928985218333075	38.0	34.0	40.0	30.0	41.0
27	35.99519625578711	38.0	34.0	40.0	30.0	41.0
28	35.890564190757296	38.0	34.0	40.0	29.0	41.0
29	35.99300935458148	38.0	34.0	40.0	30.0	41.0
30	35.54891964105067	38.0	34.0	40.0	28.0	41.0
31	35.702182735679514	38.0	34.0	40.0	29.0	41.0
32	35.77006295895171	38.0	34.0	40.0	29.0	41.0
33	35.772446234959475	38.0	34.0	40.0	29.0	41.0
34	35.74079418730586	38.0	34.0	40.0	29.0	41.0
35	35.726080954024496	38.0	34.0	40.0	29.0	41.0
36	35.475499268057554	38.0	34.0	40.0	28.0	41.0
37	35.55617360722659	38.0	34.0	40.0	28.0	41.0
38	35.45297567332754	38.0	34.0	40.0	28.0	41.0
39	35.378795091820095	38.0	34.0	40.0	28.0	41.0
40	35.293656201278225	38.0	34.0	40.0	27.0	41.0
41	35.16888530521405	37.0	34.0	40.0	27.0	41.0
42	35.36700665294027	38.0	34.0	40.0	28.0	41.0
43	35.33153868583602	37.0	34.0	40.0	28.0	41.0
44	35.2414324054128	37.0	34.0	40.0	28.0	41.0
45	35.31979487759304	37.0	34.0	40.0	28.0	41.0
46	35.22134861883056	37.0	34.0	40.0	28.0	41.0
47	35.18231168846626	37.0	34.0	40.0	28.0	41.0
48	35.10836764933411	37.0	34.0	40.0	28.0	41.0
49	35.0916832295919	37.0	34.0	40.0	28.0	41.0
50	34.90750746819323	37.0	33.0	40.0	27.0	41.0
51	33.76834616712091	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	13.0
11	12.0
12	14.0
13	24.0
14	29.0
15	37.0
16	95.0
17	170.0
18	338.0
19	647.0
20	971.0
21	1495.0
22	2314.0
23	3422.0
24	4687.0
25	6109.0
26	8139.0
27	10337.0
28	12877.0
29	15881.0
30	20618.0
31	26057.0
32	33471.0
33	43726.0
34	59776.0
35	63430.0
36	78030.0
37	99008.0
38	119734.0
39	60717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.151994096854434	4.504123870844888	54.49906573200195	17.844816300298728
2	20.72155838282375	3.612403746593195	54.42363995572641	21.242397914856646
3	21.828546945479214	3.8068445544672294	52.64689430275043	21.71771419730312
4	20.229728764742987	5.169715435059448	47.40636492388989	27.19419087630768
5	18.008164431167657	6.01888768551468	50.552229746616995	25.420718136700664
6	22.647816669245326	6.469805886483463	52.49262106804089	18.389756376230316
7	86.09978815324375	2.463016078930769	7.734043059638432	3.7031527081870443
8	88.01012817918902	2.4506682694024255	5.773716720421789	3.7654868309867537
9	82.21305475881604	5.048766409197482	7.752490389536199	4.985688442450281
10	40.939831950775385	26.832533949037764	16.26905728193471	15.958576818252146
11	30.19203075348417	25.763183890125323	23.824577794175404	20.22020756221511
12	26.970442616902513	22.168334860692905	29.964712043131048	20.896510479273534
13	24.463986051438297	22.752401128262502	31.382776144627066	21.40083667567214
14	20.901271080537473	24.953286600097595	31.820751460909513	22.324690858455423
15	19.69698772955024	24.32087047594111	34.24121966604382	21.740922128464828
16	23.02360663151756	23.20019518465182	32.05535984194804	21.72083834188258
17	23.329475262725683	24.029432417314307	28.673547719076918	23.96754460088309
18	23.967247063304097	23.525701296073695	29.988663818240248	22.518387822381968
19	25.12838746533687	24.97069254846887	27.530408340573416	22.37051164562084
20	27.389673065708198	25.843072730085808	27.1138557299787	19.653398474227295
21	27.263963438582294	25.414172309962744	27.41079823381693	19.911066017638028
22	25.47397736334099	23.560215655237258	28.003790628756413	22.96201635266534
23	24.138182402437426	25.0709627125906	28.048570034395343	22.742284850576628
24	23.737101745950515	24.511145757709198	29.19141187531985	22.560340621020437
25	24.031068873998787	25.583768729990595	26.974161836639965	23.41100055937065
26	22.833926424907467	26.88638825083608	26.82078121466741	23.458904109589042
27	22.078180974257048	26.345018625852447	28.83511062447187	22.741689775418635
28	21.05033740761458	26.108178712971448	29.096051081251563	23.74543279816241
29	22.394909727098533	25.066797186484653	28.972424217178627	23.565868869238184
30	22.711192173571522	24.509062994656226	29.07090915582638	23.70883567594587
31	23.365477309784225	25.61367125667972	27.97240041417231	23.048451019363743
32	24.357616366947145	25.74518286659605	27.316478821275126	22.580721945181676
33	23.939873606036443	25.5046237339776	27.23406091189317	23.321441748092784
34	22.18217035811623	24.894820465824832	28.926157123644717	23.99685205241422
35	22.78617164347857	25.09788986348976	28.5329612130012	23.582977280030466
36	23.70913321352487	26.485902669507162	27.150155314616235	22.654808802351738
37	22.66730538066958	26.77124120776454	28.22010044868667	22.341352962879213
38	22.319930257191483	26.330141746902637	27.948746176642107	23.401181819263773
39	22.593516061078514	25.234608381038527	28.260565559430155	23.911309998452804
40	23.645311402830178	23.899110957713958	29.229794223010362	23.2257834164455
41	21.243736833962128	24.68699046689597	29.178766528212513	24.890506170929388
42	22.115521940421075	24.88738202634993	28.643050117229805	24.35404591599919
43	22.364114587672425	24.34854147078776	28.79241398188591	24.494929959653906
44	22.27842376492151	24.367286338264524	28.50231484236459	24.851975054449376
45	22.063899170465227	23.924104114349642	27.864840579365175	26.147156135819955
46	21.87154112564417	24.914457946038585	28.60957713959273	24.604423788724517
47	20.84250740868572	25.3232745795794	29.986134748818777	23.848083262916106
48	21.35486711971722	24.73504278590386	29.845845780322055	24.064244314056864
49	22.317549956559514	23.804642776382657	29.70749080608881	24.170316460969023
50	20.88966711495662	23.95980862382919	29.595170370017733	25.555353891196457
51	20.761577187198743	23.86891089344584	28.782446472989538	26.587065446365877
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122.0
1	397.5
2	673.0
3	1397.0
4	2121.0
5	1552.5
6	984.0
7	1067.0
8	1150.0
9	1232.5
10	1315.0
11	1356.0
12	1397.0
13	1399.0
14	1401.0
15	1433.5
16	1466.0
17	1386.0
18	1306.0
19	1491.5
20	1677.0
21	1920.0
22	2163.0
23	2416.5
24	2670.0
25	3636.0
26	5385.5
27	6169.0
28	7319.5
29	8470.0
30	9582.0
31	10694.0
32	13159.5
33	15625.0
34	17394.5
35	19164.0
36	21365.0
37	23566.0
38	24992.5
39	26419.0
40	29507.5
41	32596.0
42	34962.0
43	37328.0
44	40069.0
45	42810.0
46	46545.5
47	50281.0
48	53659.0
49	57037.0
50	56284.5
51	55532.0
52	50501.0
53	45470.0
54	43079.0
55	40688.0
56	38864.0
57	37040.0
58	35348.0
59	33656.0
60	31759.5
61	29863.0
62	26796.0
63	23729.0
64	20752.5
65	17776.0
66	14908.5
67	12041.0
68	10056.0
69	8071.0
70	6947.5
71	5824.0
72	4844.0
73	3864.0
74	3341.5
75	2069.0
76	1319.0
77	1048.5
78	778.0
79	553.0
80	328.0
81	235.0
82	142.0
83	86.5
84	31.0
85	17.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.40589034561102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.15784103511066	25.514665469212705
2	8.681366879921452	5.9738031384119346
3	3.018314372221217	3.1154337995767487
4	1.6514060031320605	2.272723754393818
5	1.2135162747575352	2.0876053940961063
6	0.9752777367668701	2.0133179320629973
7	0.8895479910462079	2.142398344596608
8	0.7794834112384683	2.1455056618634853
9	0.7423720307645232	2.298777361751948
>10	7.760782880682125	47.43239510932791
>50	0.0902317346162843	2.095690341525768
>100	0.0372315409683359	2.0343537977732176
>500	0.0013140543871177374	0.3324531059995346
>1k	0.0013140543871177374	0.5408767894072211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	1434	0.2133344441402949	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.16662104423788723	No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	1035	0.1539756971305476	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	809	0.12035395070397392	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	791	0.11767611249300786	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16557966271140045	0.0
2	0.0	0.0	0.0	0.6682694024255263	0.0
3	0.0	0.0	0.0	0.9110600668864478	0.0
4	0.0	0.0	0.0	1.279560358473275	0.0
5	0.0	0.0	0.0	1.8768670483082013	0.0
6	0.0	0.0	0.0	2.3023457862728063	0.0
7	0.0	0.0	0.0	2.71056734465563	0.0
8	0.0	0.0	0.0	3.2828808778548733	0.0
9	0.0	0.0	0.0	3.5688144912702473	0.0
10	0.0	0.0	0.0	4.109440272306392	0.0
11	0.0	0.0	0.0	5.0986039536793495	0.0
12	1.487687894981136E-4	0.0	0.0	5.999398974090427	0.0
13	1.487687894981136E-4	0.0	0.0	6.305118836509051	0.0
14	1.487687894981136E-4	0.0	0.0	6.416546659843138	0.0
15	1.487687894981136E-4	0.0	0.0	6.585101698344501	0.0
16	1.487687894981136E-4	0.0	0.0	6.9835045166204495	0.0
17	1.487687894981136E-4	0.0	0.0	7.573819073348964	0.0
18	1.487687894981136E-4	0.0	0.0	8.212483486664366	0.0
19	1.487687894981136E-4	0.0	0.0	8.655070635421254	0.0
20	1.487687894981136E-4	0.0	0.0	9.146900253502018	0.0
21	1.487687894981136E-4	0.0	0.0	9.819037644454495	0.0
22	1.487687894981136E-4	0.0	0.0	10.524052937886054	0.0
23	1.487687894981136E-4	0.0	0.0	11.207199219261392	0.0
24	1.487687894981136E-4	0.0	0.0	11.76106542256287	0.0
25	2.975375789962272E-4	0.0	0.0	12.245010294800233	0.0
26	2.975375789962272E-4	0.0	0.0	12.697713721242993	0.0
27	2.975375789962272E-4	0.0	0.0	13.14744177189579	0.0
28	2.975375789962272E-4	0.0	0.0	13.609815169655928	0.0
29	2.975375789962272E-4	0.0	0.0	14.111612296633064	0.0
30	2.975375789962272E-4	0.0	0.0	14.69552979541316	0.0
31	2.975375789962272E-4	0.0	0.0	15.227080680289921	0.0
32	2.975375789962272E-4	0.0	0.0	15.735274865215477	0.0
33	2.975375789962272E-4	0.0	0.0	16.261470073670303	0.0
34	2.975375789962272E-4	0.0	0.0	16.786623900598645	0.0
35	2.975375789962272E-4	0.0	0.0	17.392856717803458	0.0
36	2.975375789962272E-4	0.0	0.0	17.921432226890257	0.0
37	2.975375789962272E-4	0.0	0.0	18.488390083667568	0.0
38	2.975375789962272E-4	0.0	0.0	19.050884876759934	0.0
39	2.975375789962272E-4	0.0	0.0	19.737006533925236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	25	3.8890703E-5	45.000004	11
TCGTTAG	20	7.0311944E-4	45.0	1
ATGATCG	20	7.0311944E-4	45.0	27
TTGTTCG	95	0.0	42.63158	1
ACACGCG	155	0.0	40.645164	36
CGTTTTT	680	0.0	40.367645	1
TTTCGCG	290	0.0	40.344826	1
GTTCGCC	45	1.9263098E-8	40.0	28
TACGAAT	90	0.0	40.0	12
TTGGGCA	985	0.0	39.28934	5
GACCGAT	385	0.0	39.155846	9
TTTACGG	75	0.0	39.0	2
CGTTTGG	515	0.0	38.883495	2
CTGTTCG	110	0.0	38.863636	1
GATAACG	35	6.244418E-6	38.571426	17
CGTAGGG	140	0.0	38.571426	3
TTACGGG	140	0.0	38.571426	3
ACGAACG	35	6.244418E-6	38.571426	21
TTTGGGC	1660	0.0	38.22289	4
TTGGGTA	655	0.0	38.129772	5
>>END_MODULE