##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934059.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	244722
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.966758199099385	31.0	30.0	31.0	26.0	34.0
2	30.128243476271034	31.0	30.0	33.0	26.0	34.0
3	30.213483054241138	31.0	30.0	34.0	25.0	34.0
4	33.969639018968465	35.0	33.0	37.0	28.0	37.0
5	34.40601989195904	35.0	35.0	37.0	32.0	37.0
6	33.8408398100702	35.0	35.0	37.0	30.0	37.0
7	34.3902509786615	35.0	35.0	37.0	32.0	37.0
8	34.48201632873219	35.0	35.0	37.0	32.0	37.0
9	35.88422373141769	37.0	35.0	39.0	31.0	39.0
10	35.25396572437296	37.0	34.0	39.0	30.0	39.0
11	35.38533928294146	37.0	35.0	39.0	30.0	39.0
12	35.31635488431772	37.0	34.0	39.0	30.0	39.0
13	35.18494046305604	37.0	34.0	39.0	29.0	39.0
14	35.86744142332933	38.0	34.0	40.0	27.0	41.0
15	36.12898717728688	38.0	34.0	40.0	30.0	41.0
16	36.35330701775893	38.0	35.0	40.0	31.0	41.0
17	36.130016917154975	38.0	34.0	40.0	30.0	41.0
18	36.25884472993846	38.0	35.0	40.0	30.0	41.0
19	36.181397667557476	38.0	34.0	40.0	30.0	41.0
20	36.22373959022891	38.0	34.0	40.0	30.0	41.0
21	35.91744918724103	38.0	34.0	40.0	29.0	41.0
22	36.1601694984513	38.0	34.0	40.0	30.0	41.0
23	36.25567787121714	38.0	34.0	40.0	30.0	41.0
24	36.14077606426884	38.0	34.0	40.0	30.0	41.0
25	35.66389208979985	38.0	34.0	40.0	29.0	41.0
26	35.73546718317111	38.0	34.0	40.0	29.0	41.0
27	35.72534549407082	38.0	34.0	40.0	29.0	41.0
28	35.67347439135018	38.0	34.0	40.0	29.0	41.0
29	35.83489020194343	38.0	34.0	40.0	30.0	41.0
30	35.37490703737302	37.0	34.0	40.0	28.0	41.0
31	35.459231291015925	37.0	34.0	40.0	29.0	41.0
32	35.43649120226216	38.0	34.0	40.0	29.0	41.0
33	35.42824511077876	38.0	34.0	40.0	29.0	41.0
34	35.38969115976496	38.0	34.0	40.0	28.0	41.0
35	35.41815611183302	38.0	34.0	40.0	28.0	41.0
36	35.20041107869338	37.0	34.0	40.0	27.0	41.0
37	35.25021044286987	37.0	34.0	40.0	28.0	41.0
38	35.12959603141524	37.0	34.0	40.0	27.0	41.0
39	35.11200872827126	37.0	34.0	40.0	27.0	41.0
40	35.02088492248347	37.0	33.0	40.0	27.0	41.0
41	34.87706867384215	37.0	33.0	40.0	27.0	41.0
42	35.11099533348044	37.0	34.0	40.0	28.0	41.0
43	35.03237142553591	37.0	33.0	40.0	27.0	41.0
44	34.960878057551014	37.0	33.0	40.0	27.0	41.0
45	35.02566994385466	37.0	34.0	40.0	27.0	41.0
46	34.92510685594266	37.0	33.0	40.0	27.0	41.0
47	34.836794403445545	36.0	33.0	40.0	27.0	41.0
48	34.83676988582963	36.0	33.0	40.0	27.0	41.0
49	34.86724936867139	36.0	33.0	40.0	27.0	41.0
50	34.64938174745221	36.0	33.0	40.0	27.0	41.0
51	33.58303299253847	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	4.0
13	2.0
14	5.0
15	9.0
16	20.0
17	42.0
18	105.0
19	188.0
20	384.0
21	612.0
22	948.0
23	1362.0
24	1772.0
25	2299.0
26	2959.0
27	3807.0
28	4912.0
29	6113.0
30	7972.0
31	10468.0
32	13402.0
33	17482.0
34	24514.0
35	25129.0
36	28680.0
37	35415.0
38	38942.0
39	17172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.077116074566245	4.534124435073267	53.49294301288809	17.895816477472398
2	19.69295772345764	6.685953857846863	52.430512990250165	21.19057542844534
3	21.623719976136186	6.2912202417436935	49.5529621366285	22.532097645491618
4	20.122016001830648	5.402865292045668	46.83886205572037	27.63625665040331
5	18.993797043175523	5.777576188491431	49.66043101968764	25.5681957486454
6	21.10108613038468	8.55133580144	51.793872230530965	18.553705837644348
7	81.50881408291858	2.2212960011768454	11.415401966312794	4.854487949591782
8	82.8062045913322	4.459345706556828	6.932355897712507	5.80209380439846
9	78.03262477423361	4.743750051078367	9.217806327179412	8.005818847508602
10	43.367576270216816	25.110533585047524	16.089685438987914	15.432204705747745
11	32.1446375887742	23.974142087756718	22.780951446947967	21.100268876521113
12	26.970194751595688	20.974820408463483	29.033352130172197	23.021632709768635
13	23.83602618481379	25.376141090707005	30.628631671856226	20.159201052622976
14	20.440745008622027	28.247153913420124	29.359436421735687	21.95266465622216
15	16.81050334665457	24.143722264447003	37.420419905035104	21.625354483863322
16	19.630029175962928	23.445378838028457	32.97251575256822	23.952076233440394
17	21.151755869925875	24.284698555912424	28.48293165305939	26.080613921102312
18	23.36365345167169	22.06258530087201	30.28947131847566	24.28428992898064
19	23.979045610938126	25.074165788118762	27.49568898586968	23.45109961507343
20	26.600796005263117	24.646333390541102	29.215599741747777	19.537270862448004
21	24.59974992031775	26.955075555119684	27.667720924150668	20.777453600411896
22	23.001201363179444	23.24147399906833	28.651694575886104	25.10563006186612
23	22.73436797672461	25.561249090805077	26.413644870506126	25.290738061964184
24	23.24678614918152	24.371736092382378	28.926291874044836	23.45518588439127
25	20.32959848317683	26.962430839891795	27.421727511216808	25.286243165714566
26	19.058360098397365	29.438710046501743	27.029854283636123	24.473075571464765
27	20.150619887055516	29.6822516978449	28.232034716944128	21.93509369815546
28	17.011956424023992	28.47394186056015	31.03562409591291	23.478477619502947
29	19.545034774151894	26.960796332164662	28.487426549309014	25.006742344374434
30	20.288735789998448	30.130106815079966	26.468809506296942	23.112347888624644
31	23.26394848031644	28.15357834604163	23.55897712506436	25.02349604857757
32	23.260679464862168	29.427268492411795	24.0301239774111	23.28192806531493
33	23.07148519544626	28.35339691568392	23.90263237469455	24.672485514175268
34	19.43184511404778	27.659957012446778	26.575869762424304	26.332328111081143
35	20.042742377064588	28.42327212101895	25.4243590686575	26.109626433258963
36	22.4164562237968	30.388767662899124	25.828082477259912	21.366693636044165
37	19.580585317217086	29.502047220928233	27.269718292593225	23.64764916926145
38	20.23234527341228	30.381412378127017	23.998251076731965	25.387991271728737
39	20.924967922785857	27.45400903882773	25.94740154134079	25.67362149704563
40	21.232255375487288	26.523156888224186	25.991941876905223	26.2526458593833
41	19.261856310425706	25.696913232157303	25.91798040225235	29.123250055164636
42	22.359657080278847	25.660136808296762	24.330056145340425	27.650149966083966
43	22.45527578231626	28.013010681508	24.80120299768717	24.73051053848857
44	19.83311676105949	26.45369030982094	27.451557277237026	26.261635651882543
45	20.508985706229925	27.44501924632849	24.814687686436038	27.23130736100555
46	21.781449971804744	27.908810813903123	26.579956031742142	23.729783182549998
47	17.515793430913444	28.775508536216606	28.99248943699381	24.71620859587614
48	20.69205057166908	26.570148985379326	26.901954053987794	25.8358463889638
49	20.83670450552055	24.20297316955566	29.66427211284641	25.296050212077375
50	20.891869141311364	25.26744632685251	26.976324155572446	26.86436037626368
51	19.62267389119082	25.41822966468074	25.268672207647864	29.690424236480577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	139.0
2	231.0
3	385.5
4	540.0
5	424.5
6	309.0
7	326.5
8	344.0
9	461.0
10	578.0
11	645.5
12	713.0
13	732.5
14	752.0
15	748.0
16	744.0
17	737.5
18	731.0
19	801.5
20	872.0
21	822.0
22	772.0
23	977.0
24	1182.0
25	1392.5
26	1887.0
27	2171.0
28	2464.5
29	2758.0
30	2760.5
31	2763.0
32	3614.5
33	4466.0
34	5137.5
35	5809.0
36	6352.0
37	6895.0
38	7527.5
39	8160.0
40	9604.5
41	11049.0
42	12513.5
43	13978.0
44	15547.0
45	17116.0
46	20405.0
47	23694.0
48	24448.0
49	25202.0
50	24777.0
51	24352.0
52	21203.5
53	18055.0
54	16757.0
55	15459.0
56	14394.5
57	13330.0
58	11958.5
59	10587.0
60	9829.5
61	9072.0
62	7741.5
63	6411.0
64	5613.0
65	4815.0
66	4082.0
67	3349.0
68	2723.5
69	2098.0
70	1768.0
71	1438.0
72	1234.0
73	1030.0
74	835.0
75	441.0
76	242.0
77	228.0
78	214.0
79	162.0
80	110.0
81	70.5
82	31.0
83	18.5
84	6.0
85	3.5
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	244722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.97052165314111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00856590218292	27.73105809857716
2	9.729759602100026	7.194285760985935
3	3.279358938933407	3.6371883198077817
4	1.716496269687759	2.53839050024109
5	1.1229621442387399	2.075824813461806
6	0.8886432716219951	1.9712163189251477
7	0.7372202265819288	1.9078791444986556
8	0.63111356728378	1.8666078243884898
9	0.6067974578612877	2.0190256699438547
>10	6.140922906880354	40.9436013108752
>50	0.08400110527770102	2.127311806866567
>100	0.04531638574191766	2.859162641691389
>500	0.004421110804089527	1.2311929454646497
>1k	0.004421110804089527	1.8972548442722763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	1309	0.5348926537050204	No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	1180	0.4821797795049076	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	1136	0.46420019450641953	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	1018	0.4159822165559287	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	977	0.3992285123527921	No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	900	0.367764238605438	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	634	0.2590694747509419	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAACACCGT	502	0.20513071975547764	No Hit
GGCTTTGGGACAGATTACTCATAGACATATAAATAAATAAATATTTTTAAA	326	0.13321237976152533	No Hit
TTTATTGGGCCACCAAAAATAAAAAAATGGACCTCTCCTGTCTTGTAGTGT	305	0.12463121419406509	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	290	0.11850181021730781	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	277	0.11318966010411816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.46501744836998715	0.0
2	0.0	0.0	0.0	1.9123740407482777	0.0
3	0.0	0.0	0.0	2.39823146263924	0.0
4	0.0	0.0	0.0	3.2113990568890416	0.0
5	0.0	0.0	0.0	4.600321998022245	0.0
6	0.0	0.0	0.0	5.182206748882406	0.0
7	0.0	0.0	0.0	5.851129036212519	0.0
8	0.0	0.0	0.0	6.708428339094973	0.0
9	0.0	0.0	0.0	7.0569871119065715	0.0
10	0.0	0.0	0.0	8.279190264871977	0.0
11	0.0	0.0	0.0	9.816036155310924	0.0
12	0.0	0.0	0.0	11.810135582415965	0.0
13	0.0	0.0	0.0	12.29190673498909	0.0
14	0.0	0.0	0.0	12.479466496677864	0.0
15	0.0	0.0	0.0	12.852951512328275	0.0
16	0.0	0.0	0.0	13.50512009545525	0.0
17	0.0	0.0	0.0	14.46457613128366	0.0
18	0.0	0.0	0.0	15.558061800737162	0.0
19	0.0	0.0	0.0	16.484419055091085	0.0
20	0.0	0.0	0.0	17.191752274008877	0.0
21	0.0	0.0	0.0	18.12709932086204	0.0
22	0.0	0.0	0.0	19.0170887782872	0.0
23	0.0	0.0	0.0	19.943037405709337	0.0
24	0.0	0.0	0.0	20.638111816673614	0.0
25	0.0	0.0	0.0	21.228577733101233	0.0
26	0.0	0.0	0.0	21.799020929871446	0.0
27	0.0	0.0	0.0	22.33636534516717	0.0
28	0.0	0.0	0.0	22.90558266114203	0.0
29	0.0	0.0	0.0	23.493596815978947	0.0
30	0.0	0.0	0.0	24.235663324098365	0.0
31	0.0	0.0	0.0	24.88578877256642	0.0
32	0.0	0.0	0.0	25.49300839319718	0.0
33	0.0	0.0	0.0	26.047923766559606	0.0
34	0.0	0.0	0.0	26.644519086963985	0.0
35	0.0	0.0	0.0	27.305677462590204	0.0
36	0.0	0.0	0.0	27.921886875720205	0.0
37	0.0	0.0	0.0	28.517664942261014	0.0
38	0.0	0.0	0.0	29.145724536412747	0.0
39	0.0	0.0	0.0	29.822410735446752	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	35	1.2066994E-7	45.000004	16
CAAACTA	20	7.0223934E-4	45.000004	36
GCGCGAC	70	0.0	45.000004	9
TTTCGTC	35	1.2066994E-7	45.000004	16
GACAATC	20	7.0223934E-4	45.000004	13
CGCGCAT	20	7.0223934E-4	45.000004	36
ATTCGTA	20	7.0223934E-4	45.000004	32
CGTTCAA	20	7.0223934E-4	45.000004	9
TCCTAAC	20	7.0223934E-4	45.000004	26
GCCGATC	20	7.0223934E-4	45.000004	9
TACGCGC	20	7.0223934E-4	45.000004	34
TCTACGG	20	7.0223934E-4	45.000004	2
CTTGCAT	20	7.0223934E-4	45.000004	38
TCGTCCC	20	7.0223934E-4	45.000004	38
AAATTCG	20	7.0223934E-4	45.000004	30
AATTCGT	20	7.0223934E-4	45.000004	31
TCACTAC	40	6.7775545E-9	45.000004	30
TCGTACA	20	7.0223934E-4	45.000004	34
CAATGAA	20	7.0223934E-4	45.000004	27
TCATATG	20	7.0223934E-4	45.000004	44
>>END_MODULE