FastQCFastQC Report
Sat 14 Jan 2017
SRR2934058.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934058.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133235
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC14871.1160731039141367No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG12090.907419221675986No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC9160.687507036439374No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC6740.5058730813975306TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT5550.4165572109430705No Hit
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC5220.3917889443464555TruSeq Adapter, Index 13 (95% over 22bp)
TGCTTTGGGAATGTGTATTTTTAAACAAATTTATGGCAATTAAAATATTTG4910.368521784816302No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT4240.31823469808984123Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC4050.30397418095845685TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA3090.23192104176830414No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC2690.20189890043907383No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT2440.18313506210830488No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT2410.1808834015086126No Hit
TTCTATGGTACTAAAATTTTGTTTTATTTTATCAAGCATTAAAGTTTTTTA2390.1793822944421511No Hit
TGGCTGGGGTAATGCTTTAAAAATAATTACTGATTCTTTTTTAGAGGTAAA1940.145607385446767No Hit
TTTCTTGGGCTTCAGTGTCCGGGTCATTGACAAAGATGGCATCCACAATCT1710.12834465418245955No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT1410.10582804818553683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGAA1700.045.00000419
TGATCCG207.0108235E-445.04
AAACTTG207.0108235E-445.019
CTGTCCG207.0108235E-445.01
CTCGTGT207.0108235E-445.016
GGTCGGC253.8721977E-545.02
TTTACGG253.8721977E-545.02
ACCATTA207.0108235E-445.05
GTCCTTG207.0108235E-445.01
TATGGTA351.2011515E-745.04
TATGGCA502.1827873E-1145.032
GACCGAT253.8721977E-545.09
TCGGGCT207.0108235E-445.05
GGATCGT253.8721977E-545.09
GATTTGA207.0108235E-445.09
CAATTAA502.1827873E-1145.037
TACGGGA253.8721977E-545.04
CGTTCAT207.0108235E-445.017
TCGTGTA207.0108235E-445.017
CTTGCGG207.0108235E-445.02
Produced by FastQC (version 0.11.5)