##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133235
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.753217998273726	31.0	30.0	31.0	25.0	33.0
2	29.69045671182497	31.0	30.0	33.0	25.0	34.0
3	29.761316470897288	31.0	30.0	33.0	25.0	34.0
4	33.7636657034563	35.0	33.0	37.0	28.0	37.0
5	34.29497504409502	35.0	35.0	37.0	32.0	37.0
6	33.541599429579314	35.0	33.0	37.0	28.0	37.0
7	34.24398243704732	35.0	35.0	37.0	31.0	37.0
8	34.00266446504297	35.0	35.0	37.0	30.0	37.0
9	35.59866401471085	37.0	35.0	39.0	30.0	39.0
10	35.124779524899616	37.0	34.0	39.0	30.0	39.0
11	35.29686643899876	37.0	34.0	39.0	30.0	39.0
12	35.192682103051	37.0	34.0	39.0	30.0	39.0
13	34.90437197433107	37.0	33.0	39.0	27.0	39.0
14	35.52857732577776	38.0	34.0	40.0	27.0	41.0
15	35.85503058505648	38.0	34.0	40.0	29.0	41.0
16	36.15234735617518	38.0	34.0	40.0	30.0	41.0
17	35.92523736255488	38.0	34.0	40.0	30.0	41.0
18	36.077337036064094	38.0	34.0	40.0	30.0	41.0
19	36.024370473224	38.0	34.0	40.0	30.0	41.0
20	35.940646226592115	38.0	34.0	40.0	29.0	41.0
21	35.70094945021954	38.0	34.0	40.0	29.0	41.0
22	35.90107704432019	38.0	34.0	40.0	30.0	41.0
23	35.939452846474275	38.0	34.0	40.0	30.0	41.0
24	35.83857094607273	38.0	34.0	40.0	29.0	41.0
25	35.43989192029122	37.0	34.0	40.0	28.0	41.0
26	35.5153450669869	37.0	34.0	40.0	29.0	41.0
27	35.43400007505535	37.0	34.0	40.0	28.0	41.0
28	35.278688032423915	37.0	34.0	40.0	27.0	41.0
29	35.46607122753031	37.0	34.0	40.0	29.0	41.0
30	35.03748264344954	37.0	34.0	40.0	27.0	41.0
31	35.20431568281608	37.0	34.0	40.0	27.0	41.0
32	35.14427140015761	37.0	34.0	40.0	27.0	41.0
33	35.145795023830075	37.0	34.0	40.0	27.0	41.0
34	35.159132360115585	37.0	34.0	40.0	27.0	41.0
35	35.19590197770856	37.0	34.0	40.0	27.0	41.0
36	34.96217210192517	37.0	33.0	40.0	27.0	41.0
37	34.960655983788044	37.0	33.0	40.0	27.0	41.0
38	34.7833902503096	37.0	33.0	40.0	26.0	41.0
39	34.78795361579164	37.0	33.0	40.0	26.0	41.0
40	34.68975869703907	37.0	33.0	40.0	26.0	41.0
41	34.659211168236574	37.0	33.0	40.0	26.0	41.0
42	34.86446504296919	37.0	33.0	40.0	27.0	41.0
43	34.77430104702218	37.0	33.0	40.0	26.0	41.0
44	34.80833864975419	37.0	33.0	40.0	26.0	41.0
45	34.85575862198371	37.0	33.0	40.0	27.0	41.0
46	34.71588546553083	37.0	33.0	40.0	26.0	41.0
47	34.57704056741847	36.0	33.0	40.0	26.0	41.0
48	34.57694299545915	36.0	33.0	40.0	26.0	41.0
49	34.663144068750704	36.0	33.0	40.0	26.0	41.0
50	34.42217885690697	36.0	33.0	40.0	26.0	41.0
51	33.32901264682703	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	3.0
12	4.0
13	6.0
14	6.0
15	13.0
16	12.0
17	32.0
18	71.0
19	137.0
20	258.0
21	481.0
22	711.0
23	947.0
24	1215.0
25	1542.0
26	1815.0
27	2231.0
28	2893.0
29	3657.0
30	4689.0
31	5943.0
32	7406.0
33	9824.0
34	13824.0
35	13282.0
36	15247.0
37	18060.0
38	20028.0
39	8883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.757458625736483	4.696964010958082	50.39366532817954	16.151912035125907
2	20.9126730964086	10.374901489848764	49.54704094269523	19.165384471047396
3	19.849138739820617	10.048410702893385	48.45198333771156	21.650467219574438
4	19.592449431455698	4.847074717604233	49.799977483394	25.760498367546063
5	22.283183848087965	6.569595076368823	47.008668893308815	24.1385521822344
6	20.08856531692123	11.475963523098285	50.7734454159943	17.66202574398619
7	78.51540511126956	2.7019927196307276	13.859721544639173	4.922880624460539
8	78.65050474725109	7.79599954966788	7.512290314106654	6.041205388974369
9	72.82921154351334	5.196832664089766	10.199271963072766	11.774683829324127
10	42.39576687807258	26.398468870792207	16.25623897624498	14.949525274890233
11	33.910008631365635	24.621908657635004	23.088527789244566	18.379554921754796
12	28.14425638908695	19.697526926108004	29.989116973768155	22.169099711036893
13	24.687957368559314	26.193567756220215	31.335610012384134	17.78286486283634
14	19.740308477502158	31.297331782189364	28.65838555935002	20.303974180958456
15	16.79288475250497	23.831575787143016	37.914962284684954	21.460577175667055
16	17.636506923856345	22.291439936953502	33.37261230157241	26.699440837617743
17	17.857169662626188	24.036476901715016	29.839006267122002	28.267347168536794
18	20.847374939017527	20.875145419747064	32.36837167410966	25.909107967125756
19	22.69373663076519	24.332945547341165	27.859046046459262	25.114271775434382
20	26.713701354749126	24.540848876046084	29.99362029496754	18.75182947423725
21	23.595151424175327	28.689908807745713	28.01290952077157	19.70203024730739
22	20.659736555709838	22.77329530528765	31.04814800915675	25.51882012984576
23	20.430817728074455	27.0717153901002	27.067212068900815	25.43025481292453
24	21.30596314782152	25.027958119112846	29.827747964123542	23.838330768942097
25	19.50238300746801	27.975381844110032	27.585094006830037	24.937141141591923
26	18.260216909971103	27.973880737043572	27.236086613877735	26.529815739107594
27	21.233159455098136	27.329905805531578	28.533043119300483	22.903891620069803
28	16.87844785529328	27.926595864450032	32.60929935827673	22.58565692197996
29	18.745825045971404	24.09126730964086	32.01335985289151	25.14954779149623
30	18.667016924982175	29.366908094719857	30.608323638683526	21.35775134161444
31	23.631928547303637	27.21131834728112	24.404998686531318	24.751754418883927
32	23.89237062333471	28.07295380343003	25.96014560738545	22.074529965849816
33	21.369009644612902	26.698690284084513	24.62716253236762	27.305137538934964
34	19.65399482118062	26.54632791683867	26.82478327766728	26.974893984313432
35	20.610950576049834	24.281907907081475	26.969640109580816	28.137501407287875
36	22.186362442301196	26.149285097759602	25.44226366945622	26.222088790482985
37	18.90043907381694	25.660674747626373	31.589297106616133	23.849589071940557
38	19.302735767628626	30.967088227567828	24.668442976695314	25.06173302810823
39	19.5339062558637	25.79652493714114	29.22280181633955	25.446766990655608
40	22.0009757195932	25.036214207978386	26.82553383120051	26.13727624122791
41	18.61522873118925	23.28817502908395	25.788268848275603	32.3083273914512
42	22.440800090066425	23.08102225391226	27.510789207040194	26.967388448981122
43	20.201148346905843	23.924644425263633	27.02142830337374	28.852778924456786
44	19.01752542500094	24.87184298420085	29.871280069050925	26.239351521747288
45	19.590948324389238	25.04747251097685	27.324651930798964	28.036927233834952
46	23.435283521597178	25.808533793672833	27.67591098435096	23.08027170037903
47	17.388073704356962	26.465268135249747	31.05265133035614	25.094006830037152
48	20.272450932562766	24.596389837505157	28.92333095658048	26.207828273351595
49	20.686756482906144	21.57616241978459	32.33009344391489	25.40698765339438
50	20.722032498967987	23.143318197170412	28.819004015461402	27.315645288400198
51	19.12785679438586	24.106278380305476	26.506548579577437	30.259316245731227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	71.5
2	110.0
3	175.0
4	240.0
5	192.5
6	145.0
7	158.5
8	172.0
9	233.5
10	295.0
11	316.5
12	338.0
13	352.0
14	366.0
15	341.0
16	316.0
17	398.0
18	480.0
19	395.0
20	310.0
21	372.5
22	435.0
23	724.0
24	1013.0
25	1052.0
26	1077.5
27	1064.0
28	1269.5
29	1475.0
30	1568.5
31	1662.0
32	1747.5
33	1833.0
34	2355.0
35	2877.0
36	3093.5
37	3310.0
38	3638.5
39	3967.0
40	4869.5
41	5772.0
42	7162.5
43	8553.0
44	9236.5
45	9920.0
46	13854.5
47	17789.0
48	16648.5
49	15508.0
50	14325.0
51	13142.0
52	11290.0
53	9438.0
54	8148.0
55	6858.0
56	6344.0
57	5830.0
58	5172.0
59	4514.0
60	4288.0
61	4062.0
62	3630.0
63	3198.0
64	2857.5
65	2517.0
66	2075.5
67	1634.0
68	1300.5
69	967.0
70	842.5
71	718.0
72	629.5
73	541.0
74	455.0
75	281.5
76	194.0
77	156.5
78	119.0
79	81.0
80	43.0
81	29.5
82	16.0
83	8.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	133235.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.38428340901415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.22941679366613	30.784703718992755
2	9.238746608184961	7.462003227380193
3	3.1929524588335876	3.8683529102713248
4	1.7098464855220608	2.7620370022891882
5	1.124409917109616	2.270424438023042
6	0.9943129019068506	2.4092768416707324
7	0.810318551834368	2.2906893834202724
8	0.7136750548265992	2.3057004540848873
9	0.6653533063227149	2.4182834840695016
>10	5.202022079322009	34.52321086801516
>50	0.07619968033304836	2.0309978609224304
>100	0.03159498940638591	2.8491012121439563
>500	0.007434115154443742	2.0017262731264305
>1k	0.003717057577221871	2.0234923255901225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	1487	1.1160731039141367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	1209	0.907419221675986	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	916	0.687507036439374	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	674	0.5058730813975306	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT	555	0.4165572109430705	No Hit
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	522	0.3917889443464555	TruSeq Adapter, Index 13 (95% over 22bp)
TGCTTTGGGAATGTGTATTTTTAAACAAATTTATGGCAATTAAAATATTTG	491	0.368521784816302	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	424	0.31823469808984123	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	405	0.30397418095845685	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA	309	0.23192104176830414	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC	269	0.20189890043907383	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	244	0.18313506210830488	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT	241	0.1808834015086126	No Hit
TTCTATGGTACTAAAATTTTGTTTTATTTTATCAAGCATTAAAGTTTTTTA	239	0.1793822944421511	No Hit
TGGCTGGGGTAATGCTTTAAAAATAATTACTGATTCTTTTTTAGAGGTAAA	194	0.145607385446767	No Hit
TTTCTTGGGCTTCAGTGTCCGGGTCATTGACAAAGATGGCATCCACAATCT	171	0.12834465418245955	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT	141	0.10582804818553683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3505085000187638	0.0
2	0.0	0.0	0.0	1.7247720193642813	0.0
3	0.0	0.0	0.0	2.193867977633505	0.0
4	0.0	0.0	0.0	3.1703381243667206	0.0
5	0.0	0.0	0.0	5.006192066649154	0.0
6	0.0	0.0	0.0	5.727474012083912	0.0
7	0.0	0.0	0.0	6.546327916838669	0.0
8	0.0	0.0	0.0	7.728449731677112	0.0
9	0.0	0.0	0.0	8.169775209216798	0.0
10	0.0	0.0	0.0	10.058918452358615	0.0
11	0.0	0.0	0.0	11.61931924794536	0.0
12	0.0	0.0	0.0	14.564491312342852	0.0
13	0.0	0.0	0.0	15.262506098247458	0.0
14	0.0	0.0	0.0	15.58299245693699	0.0
15	0.0	0.0	0.0	16.188689158254213	0.0
16	0.0	0.0	0.0	16.987278117611737	0.0
17	0.0	0.0	0.0	18.075580740796337	0.0
18	0.0	0.0	0.0	19.440087064209855	0.0
19	0.0	0.0	0.0	20.689758697039068	0.0
20	0.0	0.0	0.0	21.504859834127668	0.0
21	0.0	0.0	0.0	22.484332194993808	0.0
22	0.0	0.0	0.0	23.394003077269485	0.0
23	0.0	0.0	0.0	24.30592562014486	0.0
24	0.0	0.0	0.0	25.019702030247306	0.0
25	0.0	0.0	0.0	25.62765039216422	0.0
26	0.0	0.0	0.0	26.157541186625135	0.0
27	0.0	0.0	0.0	26.703944158817126	0.0
28	0.0	0.0	0.0	27.26310654107404	0.0
29	0.0	0.0	0.0	27.866551581791573	0.0
30	0.0	0.0	0.0	28.58858408075956	0.0
31	0.0	0.0	0.0	29.18302247907832	0.0
32	0.0	0.0	0.0	29.778211430930313	0.0
33	0.0	0.0	0.0	30.378654257514917	0.0
34	0.0	0.0	0.0	30.980598191165985	0.0
35	0.0	0.0	0.0	31.666604120538896	0.0
36	0.0	0.0	0.0	32.22126318159643	0.0
37	0.0	0.0	0.0	32.773670582054265	0.0
38	0.0	0.0	0.0	33.32908019664502	0.0
39	0.0	0.0	0.0	33.98131121702255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGAA	170	0.0	45.000004	19
TGATCCG	20	7.0108235E-4	45.0	4
AAACTTG	20	7.0108235E-4	45.0	19
CTGTCCG	20	7.0108235E-4	45.0	1
CTCGTGT	20	7.0108235E-4	45.0	16
GGTCGGC	25	3.8721977E-5	45.0	2
TTTACGG	25	3.8721977E-5	45.0	2
ACCATTA	20	7.0108235E-4	45.0	5
GTCCTTG	20	7.0108235E-4	45.0	1
TATGGTA	35	1.2011515E-7	45.0	4
TATGGCA	50	2.1827873E-11	45.0	32
GACCGAT	25	3.8721977E-5	45.0	9
TCGGGCT	20	7.0108235E-4	45.0	5
GGATCGT	25	3.8721977E-5	45.0	9
GATTTGA	20	7.0108235E-4	45.0	9
CAATTAA	50	2.1827873E-11	45.0	37
TACGGGA	25	3.8721977E-5	45.0	4
CGTTCAT	20	7.0108235E-4	45.0	17
TCGTGTA	20	7.0108235E-4	45.0	17
CTTGCGG	20	7.0108235E-4	45.0	2
>>END_MODULE