##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934057.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90626
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.78426720808598	31.0	30.0	31.0	25.0	34.0
2	29.729117471807207	31.0	30.0	33.0	25.0	34.0
3	29.77347560302783	31.0	30.0	33.0	25.0	34.0
4	33.81056209034935	35.0	33.0	37.0	28.0	37.0
5	34.30069737161521	35.0	35.0	37.0	32.0	37.0
6	33.665769205305324	35.0	33.0	37.0	28.0	37.0
7	34.373215192108226	35.0	35.0	37.0	32.0	37.0
8	34.14140533621698	35.0	35.0	37.0	30.0	37.0
9	35.6859731202966	37.0	35.0	39.0	30.0	39.0
10	35.20404740361486	37.0	34.0	39.0	30.0	39.0
11	35.32295367775252	37.0	34.0	39.0	30.0	39.0
12	35.135524021803896	37.0	34.0	39.0	30.0	39.0
13	34.85484298104297	37.0	33.0	39.0	27.0	39.0
14	35.38953501202745	38.0	33.0	40.0	27.0	41.0
15	35.68698828150862	38.0	34.0	40.0	27.0	41.0
16	36.08904729327125	38.0	34.0	40.0	30.0	41.0
17	35.898020435636575	38.0	34.0	40.0	30.0	41.0
18	36.03519961159049	38.0	34.0	40.0	30.0	41.0
19	36.03621477280251	38.0	34.0	40.0	30.0	41.0
20	35.9211925937369	38.0	34.0	40.0	29.0	41.0
21	35.7215589345221	38.0	34.0	40.0	29.0	41.0
22	35.94948469534129	38.0	34.0	40.0	30.0	41.0
23	35.92664356807097	38.0	34.0	40.0	30.0	41.0
24	35.83127358594664	38.0	34.0	40.0	29.0	41.0
25	35.48703462582482	37.0	34.0	40.0	29.0	41.0
26	35.5431222827886	37.0	34.0	40.0	29.0	41.0
27	35.40995961423874	37.0	34.0	40.0	28.0	41.0
28	35.327323284708584	37.0	34.0	40.0	27.0	41.0
29	35.50849645797012	37.0	34.0	40.0	29.0	41.0
30	35.13742193189592	37.0	34.0	40.0	27.0	41.0
31	35.31844062410346	37.0	34.0	40.0	29.0	41.0
32	35.27006598547878	37.0	34.0	40.0	28.0	41.0
33	35.188930329044645	37.0	34.0	40.0	27.0	41.0
34	35.168936066912366	37.0	34.0	40.0	27.0	41.0
35	35.167512634343346	37.0	34.0	40.0	27.0	41.0
36	34.96079491536645	37.0	33.0	40.0	27.0	41.0
37	34.91569748195882	37.0	33.0	40.0	27.0	41.0
38	34.902478317480636	37.0	33.0	40.0	27.0	41.0
39	34.911912696135765	37.0	33.0	40.0	27.0	41.0
40	34.859488447024034	37.0	33.0	40.0	26.0	41.0
41	34.72069825436409	37.0	33.0	40.0	26.0	41.0
42	34.93267936353806	37.0	33.0	40.0	27.0	41.0
43	34.86649526625913	37.0	33.0	40.0	27.0	41.0
44	34.84561825524684	37.0	33.0	40.0	27.0	41.0
45	34.96622382097853	37.0	33.0	40.0	27.0	41.0
46	34.86181669719507	37.0	33.0	40.0	27.0	41.0
47	34.694767506013726	36.0	33.0	40.0	26.0	41.0
48	34.768079800498754	36.0	33.0	40.0	27.0	41.0
49	34.84061968971377	36.0	33.0	40.0	27.0	41.0
50	34.5998278639684	36.0	33.0	40.0	26.0	41.0
51	33.553825612958754	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	1.0
12	3.0
13	1.0
14	2.0
15	1.0
16	12.0
17	22.0
18	35.0
19	85.0
20	178.0
21	258.0
22	390.0
23	546.0
24	734.0
25	903.0
26	1196.0
27	1564.0
28	1870.0
29	2548.0
30	3229.0
31	4137.0
32	5405.0
33	6840.0
34	9495.0
35	9205.0
36	10137.0
37	12239.0
38	13627.0
39	5960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.291858848454083	4.25926334605963	52.970449981241586	17.478427824244697
2	17.540220245845564	10.417540220245845	51.236951868117316	20.805287665791276
3	18.49910621675899	10.46498797254651	51.0570917838148	19.978814026879704
4	18.901860393264627	4.396089422461545	51.09019486681527	25.611855317458566
5	23.10926224262353	5.000772405270011	48.12746893827378	23.762496413832675
6	19.158961004568226	10.47933264184671	51.39363979431951	18.968066559265555
7	75.73323328846027	2.251009644031514	16.16644230132633	5.849314766181891
8	76.55087943857171	7.09068037869927	8.845143777723832	7.513296405005186
9	70.94211374219319	5.15856376757222	11.60925120826253	12.290071281972061
10	41.60836845938252	24.878070310948296	18.221040319555097	15.292520910114096
11	33.86114360117406	23.804426985633263	23.857391918434004	18.477037494758676
12	28.28327411559596	20.18184626928255	30.505594421027077	21.02928519409441
13	24.14428530443802	27.001081367378017	31.50310065544104	17.351532672742923
14	18.39207291505749	32.12102487144969	29.256504755809594	20.230397457683225
15	13.538057511089532	25.35365127005495	41.55319665438174	19.55509456447377
16	15.091695539911285	24.09573411603734	34.19769161167876	26.61487873237261
17	16.80202149493523	24.422351201641913	31.25151722463752	27.524110078785334
18	18.136075739853904	23.722772714232118	33.27411559596584	24.86703594994814
19	20.361706353585063	24.80082978394721	30.336768697724715	24.50069516474301
20	24.761106084346657	24.94869022134928	31.803235274645246	18.486968419658815
21	22.188996535210645	29.728775406616204	28.974025114205638	19.108202943967516
22	18.891929468364488	24.526074195043364	29.89870456601858	26.683291770573565
23	18.5498642773597	29.87001522741818	27.603557477986453	23.976563017235673
24	21.33493699379869	25.035861673250505	29.544501577913625	24.084699755037185
25	17.657184472447202	29.157195506808204	27.879416502990313	25.306203517754287
26	19.252753073069538	27.148941804780087	29.55443250281376	24.043872619336614
27	19.54074989517357	28.823957804603534	30.546421556727648	21.088870743495246
28	15.24507315781343	28.040518173592567	35.71712312140004	20.99728554719396
29	20.722529958290114	24.211594906538963	31.112484276035573	23.953390859135347
30	18.10848983735352	26.952530178977334	32.47302098735462	22.465958996314523
31	23.20857149162492	25.76633637146073	29.128506168207803	21.896585968706553
32	25.81378412376139	29.07443779930704	24.63862467724494	20.473153399686623
33	23.264846732725708	25.059033831350824	26.741773883874387	24.93434555204908
34	22.289409220312052	25.347030653454862	29.160505815108245	23.203054311124845
35	21.18376624809657	25.59640721205835	27.89817491669058	25.3216516231545
36	25.895438395162536	23.878357204334296	29.860084302518043	20.366120097985128
37	20.50404961048706	25.79392227396112	34.19769161167876	19.50433650387306
38	19.997572440579965	27.94672610509125	27.97431200759164	24.081389446737138
39	24.562487586343877	24.288835433540044	29.48712290071282	21.66155407940326
40	21.289696113698056	24.95089709354931	29.090989340807273	24.668417451945356
41	19.937986891179133	25.034758237150484	29.07554123540706	25.951713636263325
42	23.416017478427825	24.940966168649172	28.043828481892614	23.599187871030388
43	25.92192086156291	22.330236356012623	28.57458124600004	23.173261536424423
44	20.448877805486283	23.800013241233202	31.412618895239774	24.338490058040737
45	20.657427228389206	23.481120208328736	28.187275174894623	27.674177388387438
46	24.487453931542824	25.64606183655905	29.386710215611412	20.47977401628672
47	18.042283671352592	24.475316134442654	34.43603380928211	23.04636638492265
48	20.302120804184227	24.508419217443116	31.45234259484033	23.73711738353232
49	22.220996182111094	20.81301171849138	34.29920773288019	22.666784366517334
50	22.055480767108776	22.18347935471057	29.33153841061064	26.429501467570017
51	18.810275196963346	23.16774435592435	27.763555712488692	30.258424734623617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20.0
1	38.5
2	57.0
3	127.0
4	197.0
5	161.0
6	125.0
7	151.5
8	178.0
9	255.5
10	333.0
11	370.0
12	407.0
13	379.5
14	352.0
15	389.0
16	426.0
17	376.5
18	327.0
19	312.5
20	298.0
21	356.0
22	414.0
23	466.5
24	519.0
25	505.5
26	718.0
27	944.0
28	907.5
29	871.0
30	1009.5
31	1148.0
32	1363.5
33	1579.0
34	1858.5
35	2138.0
36	2298.5
37	2459.0
38	2832.5
39	3206.0
40	3906.0
41	4606.0
42	5171.0
43	5736.0
44	6654.0
45	7572.0
46	10356.5
47	13141.0
48	12397.5
49	11654.0
50	10733.5
51	9813.0
52	7940.5
53	6068.0
54	5042.5
55	4017.0
56	3602.5
57	3188.0
58	2896.0
59	2604.0
60	2238.0
61	1872.0
62	1618.5
63	1365.0
64	1129.5
65	894.0
66	713.5
67	533.0
68	483.0
69	433.0
70	356.0
71	279.0
72	228.5
73	178.0
74	128.0
75	61.5
76	45.0
77	42.0
78	39.0
79	28.0
80	17.0
81	10.0
82	3.0
83	1.5
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	90626.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.954273608015356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.18200532208667	33.04570432326264
2	10.247527238037858	9.008452320526118
3	3.7631169352814178	4.96215214176947
4	2.1715117738615253	3.81788890605345
5	1.41336546668675	3.1061726215434864
6	1.142240297233519	3.0123805530421732
7	0.9137922377868153	2.8115551828393617
8	0.8108650901240146	2.851278882439918
9	0.7255108701109605	2.8700372961401803
>10	3.514585529949289	24.04718292763666
>50	0.06778129236330774	1.9762540551276675
>100	0.03263543706381483	3.0785867190431
>500	0.010041672942712255	3.0311389667424358
>1k	0.005020836471356128	2.381215103833337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	1138	1.25571028181758	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	1020	1.125504822015757	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	820	0.9048176020126675	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	791	0.8728179551122194	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	583	0.6433032463090063	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	553	0.6102001633085428	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGCTAGT	429	0.47337408690662724	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	385	0.42482289850594757	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTA	279	0.30785867190431	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTC	255	0.28137620550393927	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	251	0.27696246110387746	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	244	0.26923840840376934	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTG	190	0.20965285900293515	No Hit
TTTTGCGGGAGGAGTGGGATTCTTTTGATATTTAGACAGTTGAATACTTTA	185	0.20413567850285788	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	134	0.14786043740207003	No Hit
CTTTGTGGGCGATTTTTTTGGGAAGGTTATTTTGTTTTTACTGTGACTCAT	124	0.13682607640191558	No Hit
TTTCTGGGGAACAAAAATCCTGTGGGCCCTGGAAAGTACGTATTTGATATT	107	0.11806766270165295	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104	0.1147573544016066	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	103	0.11365391830159115	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6984750513097786	0.0
2	0.0	0.0	0.0	2.8457617019398405	0.0
3	0.0	0.0	0.0	3.6556837993511797	0.0
4	0.0	0.0	0.0	4.97428993886964	0.0
5	0.0	0.0	0.0	7.491227683004877	0.0
6	0.0	0.0	0.0	8.37287312691722	0.0
7	0.0	0.0	0.0	9.584445964734183	0.0
8	0.0	0.0	0.0	11.283737558757972	0.0
9	0.0	0.0	0.0	11.896144594266547	0.0
10	0.0	0.0	0.0	14.225498201399157	0.0
11	0.0	0.0	0.0	16.40919824332973	0.0
12	0.0	0.0	0.0	20.060468298280846	0.0
13	0.0	0.0	0.0	20.84721823759186	0.0
14	0.0	0.0	0.0	21.16500783439631	0.0
15	0.0	0.0	0.0	21.848034780305873	0.0
16	0.0	0.0	0.0	22.814644803919403	0.0
17	0.0	0.0	0.0	24.31642133604043	0.0
18	0.0	0.0	0.0	26.118332487365656	0.0
19	0.0	0.0	0.0	27.61790214728665	0.0
20	0.0	0.0	0.0	28.695959217001743	0.0
21	0.0	0.0	0.0	29.84794650541787	0.0
22	0.0	0.0	0.0	30.998830357733983	0.0
23	0.0	0.0	0.0	32.12212830754971	0.0
24	0.0	0.0	0.0	32.9353607132611	0.0
25	0.0	0.0	0.0	33.65149074217112	0.0
26	0.0	0.0	0.0	34.30913865778033	0.0
27	0.0	0.0	0.0	34.95464877628937	0.0
28	0.0	0.0	0.0	35.56926268399797	0.0
29	0.0	0.0	0.0	36.25559993820758	0.0
30	0.0	0.0	0.0	37.083177013219164	0.0
31	0.0	0.0	0.0	37.8500651027299	0.0
32	0.0	0.0	0.0	38.56840200383996	0.0
33	0.0	0.0	0.0	39.19956745304879	0.0
34	0.0	0.0	0.0	39.751285503056515	0.0
35	0.0	0.0	0.0	40.54244918676759	0.0
36	0.0	0.0	0.0	41.23982080197736	0.0
37	0.0	0.0	0.0	41.8996755897866	0.0
38	0.0	0.0	0.0	42.680908348597534	0.0
39	0.0	0.0	0.0	43.3286253393066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGAT	45	3.765308E-10	45.000004	5
TACGGCC	25	3.8623264E-5	45.000004	7
TTCGACG	25	3.8623264E-5	45.000004	25
TTTTAAG	25	3.8623264E-5	45.000004	45
CGTGGGA	25	3.8623264E-5	45.000004	4
CGGGCAC	25	3.8623264E-5	45.000004	6
TCTGCGG	50	2.1827873E-11	45.000004	2
CGGGATT	45	3.765308E-10	45.000004	6
GACTGTA	25	3.8623264E-5	45.000004	39
GGGACCC	25	3.8623264E-5	45.000004	7
GGAGGGT	25	3.8623264E-5	45.000004	39
CCGGGAT	25	3.8623264E-5	45.000004	5
ACGGCCT	25	3.8623264E-5	45.000004	8
CGACCTG	25	3.8623264E-5	45.000004	12
AGGAGTG	50	2.1827873E-11	45.000004	10
CTCCGGG	25	3.8623264E-5	45.000004	3
TTAGACA	35	1.1954398E-7	45.0	32
TCCTCGG	20	6.998895E-4	45.0	2
GGGTCAA	20	6.998895E-4	45.0	7
TTCGCTG	20	6.998895E-4	45.0	1
>>END_MODULE