##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520527
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.90428354340889	31.0	30.0	31.0	25.0	34.0
2	29.834648346771637	31.0	30.0	33.0	25.0	34.0
3	29.905876160122336	31.0	30.0	33.0	25.0	34.0
4	33.889973046547055	35.0	33.0	37.0	28.0	37.0
5	34.393191899747755	35.0	35.0	37.0	32.0	37.0
6	33.73102451938132	35.0	33.0	37.0	28.0	37.0
7	34.45094490775695	35.0	35.0	37.0	32.0	37.0
8	34.25045002468652	35.0	35.0	37.0	30.0	37.0
9	35.843266535645604	37.0	35.0	39.0	31.0	39.0
10	35.22710445375552	37.0	34.0	39.0	30.0	39.0
11	35.32143961792568	37.0	34.0	39.0	30.0	39.0
12	35.422965571430495	37.0	35.0	39.0	30.0	39.0
13	35.419980135516504	37.0	35.0	39.0	30.0	39.0
14	36.10835172815243	38.0	35.0	40.0	29.0	41.0
15	36.270573860721925	38.0	35.0	40.0	30.0	41.0
16	36.508866975200135	38.0	35.0	40.0	31.0	41.0
17	36.17931634670248	38.0	34.0	40.0	30.0	41.0
18	36.305029326048405	38.0	35.0	40.0	30.0	41.0
19	36.27774543875726	38.0	34.0	40.0	30.0	41.0
20	36.16871363060898	38.0	34.0	40.0	30.0	41.0
21	36.01203972128247	38.0	34.0	40.0	30.0	41.0
22	36.235711115849895	38.0	34.0	40.0	30.0	41.0
23	36.17665750287689	38.0	34.0	40.0	30.0	41.0
24	36.09431018948104	38.0	34.0	40.0	30.0	41.0
25	35.71638935156101	38.0	34.0	40.0	29.0	41.0
26	35.81059772115567	38.0	34.0	40.0	29.0	41.0
27	35.683290203966365	38.0	34.0	40.0	29.0	41.0
28	35.608106784086125	38.0	34.0	40.0	28.0	41.0
29	35.755282627029914	38.0	34.0	40.0	29.0	41.0
30	35.31059099720091	38.0	34.0	40.0	27.0	41.0
31	35.53971455851474	38.0	34.0	40.0	29.0	41.0
32	35.424640796731005	38.0	34.0	40.0	27.0	41.0
33	35.40832848248026	38.0	34.0	40.0	27.0	41.0
34	35.34886758996171	38.0	34.0	40.0	27.0	41.0
35	35.266841105264476	38.0	34.0	40.0	27.0	41.0
36	35.088773493017655	38.0	33.0	40.0	27.0	41.0
37	35.08724043133209	38.0	34.0	40.0	27.0	41.0
38	35.112059508920765	37.0	33.0	40.0	27.0	41.0
39	35.03350642714019	37.0	33.0	40.0	27.0	41.0
40	34.9244803823817	37.0	33.0	40.0	26.0	41.0
41	34.68665794473678	37.0	33.0	40.0	25.0	41.0
42	34.78385943476515	37.0	33.0	40.0	26.0	41.0
43	34.91491891871123	37.0	33.0	40.0	26.0	41.0
44	34.9967475270255	37.0	33.0	40.0	27.0	41.0
45	35.054781020004725	37.0	34.0	40.0	27.0	41.0
46	34.92248432838258	37.0	33.0	40.0	26.0	41.0
47	34.723816439877275	37.0	33.0	40.0	26.0	41.0
48	34.69702820410853	37.0	33.0	40.0	26.0	41.0
49	34.706866310489175	37.0	33.0	40.0	26.0	41.0
50	34.54750858264797	37.0	33.0	40.0	26.0	41.0
51	33.428413895917025	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	7.0
11	5.0
12	4.0
13	7.0
14	13.0
15	19.0
16	47.0
17	116.0
18	224.0
19	468.0
20	1005.0
21	1874.0
22	2695.0
23	3568.0
24	4440.0
25	5454.0
26	6462.0
27	8138.0
28	10373.0
29	13322.0
30	17614.0
31	22541.0
32	27820.0
33	35530.0
34	46334.0
35	50573.0
36	61726.0
37	76272.0
38	85944.0
39	37926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.220401631423538	4.2843118608640856	54.47901837945006	16.01626812826232
2	18.579631796237276	10.113212186879835	52.228222551375815	19.078933465507074
3	18.734666981731976	9.97047223294853	51.40424992363508	19.890610861684408
4	18.204050894574152	4.373644402691887	52.20343997525585	25.218864727478113
5	22.236310508388613	4.8508530777462076	49.06738747461707	23.845448939248108
6	20.81582703682998	10.791563165791592	50.116516530362496	18.276093267015927
7	79.04700428604087	1.824881322198464	14.733721785805539	4.394392605955119
8	80.07461668655036	7.075329425755052	7.150253493094498	5.699800394600088
9	75.42317689572299	4.60494844647828	9.076570475690984	10.895304182107749
10	39.525135103462446	32.11207103570036	15.067229941962665	13.295563918874526
11	29.479738803174477	24.97353643518972	27.229135088093415	18.31758967354239
12	25.57753968574157	20.144392125672635	30.991860172479047	23.286208016106755
13	25.64958205818334	21.876290759172914	33.451290711144665	19.022836471499076
14	19.98801214922569	28.14071124072334	29.41845475835067	22.452821851700296
15	16.79567054158574	25.131837541568448	35.8519346738978	22.220557242948015
16	19.029944652246666	25.005042966070924	30.854499382356725	25.110512999325685
17	19.89041874869123	26.65855949835455	30.135036222904866	23.315985530049353
18	20.060054521667464	24.628117273455555	30.346936854380274	24.964891350496707
19	20.99583691143783	27.745919039742418	27.786454881302987	23.471789167516764
20	26.001725174678743	26.82685048037854	30.174611499499544	16.996812845443177
21	24.333800167906755	28.999648433222486	27.772238519807814	18.894312879062948
22	20.51478597651995	25.10647862646894	30.07490485604013	24.303830540970978
23	21.819809539178564	28.454623871576306	27.467547312627396	22.258019276617734
24	22.471456812038568	26.525233081088974	27.501551312419913	23.501758794452545
25	20.601621049436435	30.43012178042638	24.860958221187374	24.107298948949815
26	21.180265384888777	27.05027789144463	28.155696054191232	23.613760669475358
27	21.95025426154647	27.58761793336369	27.85369442891531	22.60843337617453
28	18.10895496295101	28.629062469381992	29.83706897048568	23.42491359718132
29	22.091457311532352	24.10153555915447	30.291992538331346	23.51501459098183
30	23.076612740549482	26.58036951013108	29.299536815573447	21.043480933745993
31	23.366511247255186	26.127559185210377	25.38312133664536	25.12280823088908
32	24.44368880000461	28.058294766649954	26.619176334753046	20.878840098592388
33	24.2817375467555	27.021076716481566	25.04711571157692	23.65007002518601
34	24.33456861987947	25.14566967707727	27.29272448883535	23.227037214207908
35	22.50027376101528	24.607561183185503	29.379071594749167	23.51309346105005
36	22.937330820495383	28.23234913846928	26.018054779098875	22.81226526193646
37	19.99089384412336	26.487963160412427	31.506915107189442	22.014227888274768
38	21.846321132237136	26.61053125006004	26.322361760292935	25.220785857409894
39	24.08578229371387	26.096436880315526	28.272884980029854	21.544895845940747
40	25.233081088973293	23.246440626518893	29.7896170611707	21.730861223337115
41	21.915097583794886	26.76479798358202	27.114827857152463	24.20527657547063
42	21.00467410912402	26.118337761537823	29.774248021716453	23.1027401076217
43	22.24860573995201	27.397618183110577	27.124433506811368	23.229342570126047
44	21.69474398061964	24.440999218100117	29.466098780658832	24.39815802062141
45	22.672599115896006	22.100678735204898	27.120591246947807	28.106130901951293
46	24.045438565146476	25.819217831159573	27.81988254211597	22.315461061577977
47	19.354423497724422	25.75716533436306	32.84651900862011	22.04189215929241
48	21.055007713336675	25.21732782353269	29.480123029160833	24.247541433969804
49	21.879364567063764	22.347736044431894	32.80790429699132	22.964995091513025
50	22.209414689343685	23.550747607712953	28.717050220257544	25.522787482685814
51	20.81505858485727	22.570395003525274	27.712683491922608	28.90186291969485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	165.0
1	313.5
2	462.0
3	1014.0
4	1566.0
5	1120.5
6	675.0
7	741.0
8	807.0
9	1097.5
10	1388.0
11	1599.5
12	1811.0
13	1928.5
14	2046.0
15	1995.5
16	1945.0
17	1911.0
18	1877.0
19	1856.0
20	1835.0
21	1920.0
22	2005.0
23	2098.0
24	2191.0
25	2595.0
26	3736.5
27	4474.0
28	5180.0
29	5886.0
30	7024.5
31	8163.0
32	8912.5
33	9662.0
34	11532.5
35	13403.0
36	14592.0
37	15781.0
38	18113.0
39	20445.0
40	22283.5
41	24122.0
42	26215.5
43	28309.0
44	31771.0
45	35233.0
46	49993.5
47	64754.0
48	61512.5
49	58271.0
50	55777.0
51	53283.0
52	45455.0
53	37627.0
54	33513.0
55	29399.0
56	26667.0
57	23935.0
58	21911.0
59	19887.0
60	17743.5
61	15600.0
62	13532.5
63	11465.0
64	9358.0
65	7251.0
66	5797.0
67	4343.0
68	3488.0
69	2633.0
70	2121.5
71	1610.0
72	1493.0
73	1376.0
74	1140.0
75	707.0
76	510.0
77	377.0
78	244.0
79	174.5
80	105.0
81	69.5
82	34.0
83	33.5
84	33.0
85	18.0
86	3.0
87	2.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	2.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	520527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.7629280855092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05506311506561	28.70591085897133
2	10.431751173964617	8.087304411246334
3	3.6863710771165348	4.2868361087630795
4	2.0096629638313965	3.116016837724308
5	1.3833792716509337	2.6811915610994626
6	1.0556384534604133	2.455178247347049
7	0.9329083042129415	2.5313580254606416
8	0.7494489579407881	2.3240668848334862
9	0.6671361907500144	2.3274136966755026
>10	4.863919093723767	30.705240312650822
>50	0.1056419909960887	2.837383705921344
>100	0.05106864019712659	3.6534711534843867
>500	0.002503364715545421	0.7768841980025498
>1k	0.004506056487981758	3.4186398070984043
>5k	0.0010013458862181684	2.0931041907213337
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGC	5431	1.0433656659500852	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCG	5354	1.028572965475374	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTC	3947	0.7582699840738328	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	3065	0.5888263240907773	TruSeq Adapter, Index 21 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTGGTAT	2266	0.43532804254150126	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1766	0.3392715459524674	TruSeq Adapter, Index 21 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1513	0.2906669586784163	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	1485	0.2852877948694304	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTA	1370	0.2631948006539526	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTC	1190	0.22861446188190046	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCT	1013	0.1946104620893825	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCT	962	0.18481269943730105	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.18116255256691777	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCC	901	0.17309380685343892	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	696	0.13371064325193507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.31525742180520894	0.0
2	0.0	0.0	0.0	1.6944365998305564	0.0
3	0.0	0.0	0.0	2.222363105083886	0.0
4	0.0	0.0	0.0	3.208479099066907	0.0
5	0.0	0.0	0.0	5.195888013494017	0.0
6	0.0	0.0	0.0	5.906321862266511	0.0
7	0.0	0.0	0.0	6.815784771971483	0.0
8	0.0	0.0	0.0	8.126955950411794	0.0
9	0.0	0.0	0.0	8.563436670912363	0.0
10	0.0	0.0	0.0	10.555840523162104	0.0
11	0.0	0.0	0.0	12.454877460727301	0.0
12	0.0	0.0	0.0	15.35501520574341	0.0
13	0.0	0.0	0.0	15.995135699012732	0.0
14	0.0	0.0	0.0	16.21779465810611	0.0
15	0.0	0.0	0.0	16.789138699817684	0.0
16	0.0	0.0	0.0	17.507641294303657	0.0
17	0.0	0.0	0.0	18.4874175595118	0.0
18	0.0	0.0	0.0	19.495434434717122	0.0
19	0.0	0.0	0.0	20.810255760027818	0.0
20	0.0	0.0	0.0	21.613672297498496	0.0
21	0.0	0.0	0.0	22.60728069821546	0.0
22	0.0	0.0	0.0	23.738249889054746	0.0
23	0.0	0.0	0.0	24.80370854922031	0.0
24	0.0	0.0	0.0	25.609622555602304	0.0
25	0.0	0.0	0.0	26.28797353451406	0.0
26	0.0	0.0	0.0	26.936354886490037	0.0
27	0.0	0.0	0.0	27.593957662138564	0.0
28	0.0	0.0	0.0	28.278648369825195	0.0
29	0.0	0.0	0.0	29.026544252267414	0.0
30	0.0	0.0	0.0	29.81228639436571	0.0
31	0.0	0.0	0.0	30.51714896633604	0.0
32	0.0	0.0	0.0	31.23008028401985	0.0
33	0.0	0.0	0.0	31.92975580517437	0.0
34	0.0	0.0	0.0	32.59965381238629	0.0
35	0.0	0.0	0.0	33.355810553535164	0.0
36	0.0	0.0	0.0	34.08718471856407	0.0
37	0.0	0.0	0.0	34.835656939985824	0.0
38	0.0	0.0	0.0	35.53955894699026	0.0
39	0.0	0.0	0.0	36.30877937167524	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	90	0.0	45.000004	40
ATGGGCG	35	1.21021E-7	45.0	5
TCGTTGC	20	7.0297264E-4	45.0	1
CGCGGAA	20	7.0297264E-4	45.0	41
ACCGCAT	20	7.0297264E-4	45.0	22
CGCATCG	25	3.887855E-5	45.0	21
CGCGCGA	20	7.0297264E-4	45.0	41
CGATTCG	40	6.8030204E-9	45.0	10
TACGGAG	20	7.0297264E-4	45.0	29
CGGGCCA	35	1.21021E-7	45.0	6
CGAATAT	55	1.8189894E-12	45.0	14
TCGTAAC	50	2.1827873E-11	45.0	34
TATAGCG	70	0.0	45.0	1
CGTAACC	50	2.1827873E-11	45.0	35
GCTACGA	55	1.8189894E-12	45.0	10
CGATCGA	85	0.0	45.0	41
GATTACG	20	7.0297264E-4	45.0	11
TCGAAGG	20	7.0297264E-4	45.0	44
TCGAACG	25	3.887855E-5	45.0	12
GCATCGC	25	3.887855E-5	45.0	22
>>END_MODULE