##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211055
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.979261329985075	31.0	30.0	31.0	26.0	34.0
2	30.271189974177346	31.0	30.0	33.0	26.0	34.0
3	30.366852242306507	31.0	30.0	34.0	26.0	34.0
4	34.04067660088602	35.0	33.0	37.0	30.0	37.0
5	34.39856909336429	35.0	35.0	37.0	32.0	37.0
6	33.97174670109687	35.0	35.0	37.0	30.0	37.0
7	34.467333159602944	35.0	35.0	37.0	32.0	37.0
8	34.58438795574613	35.0	35.0	37.0	32.0	37.0
9	35.93019828954538	37.0	35.0	39.0	31.0	39.0
10	35.20335931392291	37.0	34.0	39.0	30.0	39.0
11	35.374561133353865	37.0	35.0	39.0	30.0	39.0
12	35.343289663831705	37.0	34.0	39.0	30.0	39.0
13	35.22902087133686	37.0	34.0	39.0	30.0	39.0
14	35.83482978370567	38.0	34.0	40.0	27.0	41.0
15	36.21827011916325	38.0	34.0	40.0	30.0	41.0
16	36.40693658051219	38.0	35.0	40.0	31.0	41.0
17	36.21478761460283	38.0	34.0	40.0	30.0	41.0
18	36.33972187344531	38.0	35.0	40.0	30.0	41.0
19	36.349738219895286	38.0	35.0	40.0	30.0	41.0
20	36.35983037596835	38.0	34.0	40.0	30.0	41.0
21	36.04078557721921	38.0	34.0	40.0	30.0	41.0
22	36.22980265807491	38.0	34.0	40.0	30.0	41.0
23	36.326066665087296	38.0	35.0	40.0	30.0	41.0
24	36.177882542465234	38.0	34.0	40.0	30.0	41.0
25	35.671270521901874	38.0	34.0	40.0	29.0	41.0
26	35.8280732510483	38.0	34.0	40.0	29.0	41.0
27	35.83956788514842	38.0	34.0	40.0	29.0	41.0
28	35.73729596550662	38.0	34.0	40.0	29.0	41.0
29	35.89316528866883	38.0	34.0	40.0	30.0	41.0
30	35.422311719693916	38.0	34.0	40.0	28.0	41.0
31	35.57845585273981	38.0	34.0	40.0	29.0	41.0
32	35.65323256970932	38.0	34.0	40.0	29.0	41.0
33	35.5979720925825	38.0	34.0	40.0	29.0	41.0
34	35.61152306270877	38.0	34.0	40.0	29.0	41.0
35	35.674715121650756	38.0	34.0	40.0	29.0	41.0
36	35.473118381464545	38.0	34.0	40.0	28.0	41.0
37	35.54580559569781	38.0	34.0	40.0	29.0	41.0
38	35.400488024448606	38.0	34.0	40.0	28.0	41.0
39	35.34915543341783	38.0	34.0	40.0	28.0	41.0
40	35.26917154296274	37.0	34.0	40.0	27.0	41.0
41	35.18824477032053	37.0	34.0	40.0	27.0	41.0
42	35.420648646087514	37.0	34.0	40.0	28.0	41.0
43	35.33209353012248	37.0	34.0	40.0	28.0	41.0
44	35.24104143469712	37.0	34.0	40.0	28.0	41.0
45	35.324957949349695	37.0	34.0	40.0	28.0	41.0
46	35.18747245978536	37.0	34.0	40.0	28.0	41.0
47	35.14390561701926	37.0	33.0	40.0	28.0	41.0
48	35.144028807656774	37.0	34.0	40.0	28.0	41.0
49	35.141877709601765	37.0	34.0	40.0	28.0	41.0
50	34.94547866669826	37.0	33.0	40.0	27.0	41.0
51	33.72085475350027	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	6.0
12	4.0
13	7.0
14	10.0
15	14.0
16	16.0
17	38.0
18	69.0
19	120.0
20	214.0
21	394.0
22	639.0
23	944.0
24	1411.0
25	1843.0
26	2595.0
27	3138.0
28	4163.0
29	5333.0
30	6823.0
31	8761.0
32	11066.0
33	14553.0
34	20181.0
35	21026.0
36	24448.0
37	31286.0
38	35857.0
39	16091.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.380635379403472	4.785956267323684	55.397408258510815	16.43600009476203
2	18.50133851365758	4.930942171471892	55.14439364146786	21.42332567340267
3	20.421217218260644	4.443865343156997	52.905166899623325	22.22975053895904
4	19.861647437871646	4.447182014166923	49.51647674776717	26.17469380019426
5	18.069223662078603	5.7620051645305725	52.12290635142498	24.045864821965836
6	20.511714955817204	6.620549145957215	53.827201440382844	19.040534457842742
7	82.44533415460425	2.0932932174077847	10.773495060529246	4.687877567458719
8	83.65970955438155	3.3365710359858802	7.27488095520125	5.728838454431309
9	79.16988462722986	4.878349245457345	9.474781455070953	6.476984672241833
10	40.57804837601573	25.340314136125652	17.648480253962237	16.433157233896377
11	29.297576461112033	27.497097912866312	24.61822747625027	18.587098149771386
12	26.249555802989743	22.47186752268366	30.904740470493476	20.37383620383312
13	22.23164577953614	27.28293572765393	31.900215583615644	18.585202909194287
14	18.105707043187795	30.093103693350077	31.991187131316483	19.810002132145648
15	15.320651015138234	26.25666295515387	39.32955864585061	19.09312738385729
16	17.584989694629364	23.42706877354244	34.97856009097155	24.009381440856647
17	18.014261685342685	24.29508895785459	30.69389495629101	26.996754400511712
18	20.194735969297103	23.011063466868826	33.897325341735566	22.896875222098505
19	22.392741228589706	24.97642794532231	30.741749780862808	21.88908104522518
20	23.610907109521214	26.55326810547014	31.127905048447087	18.70791973656156
21	23.309563857762193	26.238658169671414	30.2129776598517	20.238800312714694
22	20.090971547700835	25.02665182061548	30.67399493023146	24.20838170145223
23	20.57615313543863	25.826443344152	29.052142806377486	24.545260714031887
24	20.6874985193433	25.49951434460212	30.778233161971997	23.034753974082587
25	18.377674066001752	27.603231385183957	29.40607898415105	24.61301556466324
26	19.4290587761484	28.452299163725097	27.887043661604793	24.23159839852171
27	17.963090189760962	28.75222098505129	29.498945772428986	23.78574305275876
28	16.54687166852242	27.76716969510317	32.40814005827865	23.277818578095758
29	18.835374665371585	25.42228329108526	30.735116438842958	25.007225604700196
30	19.13245362583213	28.2097083698562	29.276728814763924	23.381109189547747
31	20.80547724526782	29.530691052095428	25.123782900191895	24.54004880244486
32	21.845964322096137	28.86640922982161	26.240079600104238	23.047546847978015
33	20.189524057710077	29.588022079552722	25.921679183151312	24.300774679585892
34	18.85574850157542	28.300206107412762	27.05740209897894	25.786643292032885
35	18.818791310321952	27.177749875624833	27.758641112506215	26.244817701546992
36	21.967259719030586	27.91926275141551	26.89062092819407	23.222856601359833
37	18.08533320698396	28.796285328468883	28.643244651867995	24.47513681267916
38	17.618630214872898	29.999289284783586	26.59259434744498	25.789486152898533
39	18.82400322190898	28.348060931984552	27.969960436852954	24.857975409253513
40	20.500817322498875	26.666034919807636	28.248086991542486	24.585060766151003
41	17.83042334936391	26.379379782521145	28.236715548079882	27.55348132003506
42	20.21368837506811	25.91694108170856	28.20260121769207	25.66676932553126
43	21.11392764919097	26.889199497761247	27.014285375849894	24.98258747719789
44	19.005946317310656	26.52578711710218	27.643505247447347	26.82476131813982
45	19.76167349742958	27.378171566653243	24.810594394825994	28.049560541091186
46	21.16509914477269	26.21544147260193	28.362749046457086	24.256710336168297
47	16.933026936106703	27.698467224183272	30.336168297363248	25.032337542346784
48	17.974935443367844	26.905782852810876	29.576176825945844	25.543104877875432
49	19.774466371325012	24.31735803463552	31.25962426855559	24.64855132548388
50	19.349458671910167	25.200540143564474	28.28362275236313	27.16637843216223
51	18.173461893819145	25.476297647532636	26.7773803037123	29.572860154935913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	110.0
2	162.0
3	278.0
4	394.0
5	308.0
6	222.0
7	244.0
8	266.0
9	337.5
10	409.0
11	470.5
12	532.0
13	571.0
14	610.0
15	624.5
16	639.0
17	590.5
18	542.0
19	541.5
20	541.0
21	644.0
22	747.0
23	995.0
24	1243.0
25	1467.5
26	1876.5
27	2061.0
28	2383.5
29	2706.0
30	3323.5
31	3941.0
32	4584.0
33	5227.0
34	6000.5
35	6774.0
36	7442.5
37	8111.0
38	8354.0
39	8597.0
40	9987.0
41	11377.0
42	12847.0
43	14317.0
44	15991.0
45	17665.0
46	19809.0
47	21953.0
48	23301.5
49	24650.0
50	22828.5
51	21007.0
52	17787.5
53	14568.0
54	12649.5
55	10731.0
56	9098.5
57	7466.0
58	7068.5
59	6671.0
60	5637.5
61	4604.0
62	4125.0
63	3646.0
64	3055.5
65	2465.0
66	1991.0
67	1517.0
68	1394.0
69	1271.0
70	1013.0
71	755.0
72	569.0
73	383.0
74	336.5
75	222.0
76	154.0
77	106.0
78	58.0
79	42.5
80	27.0
81	16.5
82	6.0
83	3.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	211055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.69344483665395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90778871978515	29.733481793845208
2	10.36944195762459	8.231977446637133
3	3.7087436586093703	4.416384354789036
4	1.8669054013727246	2.9641562625855817
5	1.1626380185019398	2.30745540262017
6	0.8928678006565204	2.1264599275070477
7	0.6410026857654432	1.781052332330435
8	0.576544315129812	1.8308023974793302
9	0.5335720680393913	1.9061382104190852
>10	5.205610265592361	37.483120513610196
>50	0.08116980005968367	2.1368837506811023
>100	0.04774694121157863	3.0641302030276467
>500	0.005968367651447329	2.0179574044680297
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	996	0.47191490369808814	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	938	0.4444339153301272	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	888	0.4207434081163678	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	859	0.4070029139323873	Illumina PCR Primer Index 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	578	0.2738622633910592	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	434	0.20563360261543198	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	340	0.16109544905356424	No Hit
TGTTGACGGGGGAAGTGACTGAATCAATTAGAAATGGAATTGTTAGCATAT	230	0.10897633318329344	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT	229	0.10850252303901828	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	229	0.10850252303901828	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	221	0.10471204188481677	No Hit
TGGGTTGGGTTTCTTTTCTAGTGTGTAACACGAGGATCTGCAGAATTTCCC	214	0.10139537087489044	No Hit
TTCTTTGGGGAATTATTTAATAGTTGTAACCTTGGTCTCTAACTTCTGTGT	213	0.10092156073061523	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44869820662860394	0.0
2	0.0	0.0	0.0	1.9933192769657198	0.0
3	0.0	0.0	0.0	2.5438866646134892	0.0
4	0.0	0.0	0.0	3.56020942408377	0.0
5	0.0	0.0	0.0	5.113832887162114	0.0
6	0.0	0.0	0.0	5.821705242709246	0.0
7	0.0	0.0	0.0	6.59733244888773	0.0
8	0.0	0.0	0.0	7.7965459240482335	0.0
9	0.0	0.0	0.0	8.306839449432612	0.0
10	0.0	0.0	0.0	9.473833834782402	0.0
11	0.0	0.0	0.0	11.387079197365615	0.0
12	0.0	0.0	0.0	13.489848617658904	0.0
13	0.0	0.0	0.0	14.105327995072374	0.0
14	0.0	0.0	0.0	14.338916396200043	0.0
15	0.0	0.0	0.0	14.702328776859112	0.0
16	0.0	0.0	0.0	15.545237023524674	0.0
17	0.0	0.0	0.0	16.883750681102082	0.0
18	0.0	0.0	0.0	18.417947928265143	0.0
19	0.0	0.0	0.0	19.3774134704224	0.0
20	0.0	0.0	0.0	20.347302835753712	0.0
21	0.0	0.0	0.0	21.534671057307335	0.0
22	0.0	0.0	0.0	22.692189239771622	0.0
23	0.0	0.0	0.0	23.78574305275876	0.0
24	0.0	0.0	0.0	24.605434602354837	0.0
25	0.0	0.0	0.0	25.323256970931748	0.0
26	0.0	0.0	0.0	25.959583994693325	0.0
27	0.0	0.0	0.0	26.588803866290778	0.0
28	0.0	0.0	0.0	27.317050058041744	0.0
29	0.0	0.0	0.0	28.039610528061406	0.0
30	0.0	0.0	0.0	28.801023429911634	0.0
31	0.0	0.0	0.0	29.48378384781218	0.0
32	0.0	0.0	0.0	30.23856340764256	0.0
33	0.0	0.0	0.0	30.913269053090428	0.0
34	0.0	0.0	0.0	31.62114140863756	0.0
35	0.0	0.0	0.0	32.369761436592356	0.0
36	0.0	0.0	0.0	33.10464097036318	0.0
37	0.0	0.0	0.0	33.79213948970647	0.0
38	0.0	0.0	0.0	34.55876430314373	0.0
39	0.0	0.0	0.0	35.2983819383573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCG	30	2.1570559E-6	45.000004	1
TAATAGT	60	0.0	45.000004	18
GGGTAAC	35	1.2056262E-7	45.000004	7
TGACCTC	30	2.1570559E-6	45.000004	11
TCGACGT	30	2.1570559E-6	45.000004	26
TTCGACG	30	2.1570559E-6	45.000004	25
GACTAAT	35	1.2056262E-7	45.000004	9
GTTGACG	55	1.8189894E-12	45.0	2
GTGCAAT	20	7.0201897E-4	45.0	21
ATGGGCG	20	7.0201897E-4	45.0	5
CGTGGGT	20	7.0201897E-4	45.0	4
CATATGC	45	3.8198777E-10	45.0	33
GGTAACA	20	7.0201897E-4	45.0	8
CCTAGCG	20	7.0201897E-4	45.0	22
CCCTACA	20	7.0201897E-4	45.0	27
CGGTTGG	25	3.8799524E-5	45.0	2
TAGCGCA	20	7.0201897E-4	45.0	24
TAAACTC	25	3.8799524E-5	45.0	10
TTCCGTT	20	7.0201897E-4	45.0	15
ATTCGAA	25	3.8799524E-5	45.0	42
>>END_MODULE