SRR2934052.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127543
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	1111	0.8710787734332734	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1003	0.7864014489231084	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	951	0.7456308852700657	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	898	0.7040762723160032	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	850	0.6664419058670409	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	847	0.6640897579639808	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	797	0.6248872929129784	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	476	0.3732074672855429	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	270	0.211693311275413	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	256	0.20071662106113233	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	246	0.19287612805093185	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	234	0.18346753643869126	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	163	0.12780003606626783	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	150	0.11760739515300724	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	138	0.10819880354076665	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	137	0.1074147542397466	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	137	0.1074147542397466	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	133	0.10427855703566641	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCAT	20	7.009689E-4	45.0	21
CGTGGGT	20	7.009689E-4	45.0	4
CAGAAGC	20	7.009689E-4	45.0	21
GGGTGAT	20	7.009689E-4	45.0	7
CCGGGTT	25	3.8712584E-5	45.0	5
CAAACTA	20	7.009689E-4	45.0	15
ACACGCG	20	7.009689E-4	45.0	36
TCCGCGG	25	3.8712584E-5	45.0	2
ATAGGTC	20	7.009689E-4	45.0	26
GAATGTC	20	7.009689E-4	45.0	27
GGAGAAT	40	6.7338988E-9	45.0	8
GGATGCG	20	7.009689E-4	45.0	12
ATGGCAA	20	7.009689E-4	45.0	11
CTATCAA	20	7.009689E-4	45.0	13
CTTTTCG	25	3.8712584E-5	45.0	1
TTTGTCG	45	3.783498E-10	45.0	1
TAGGTCC	20	7.009689E-4	45.0	27
AGCTTAG	20	7.009689E-4	45.0	36
GGCCGAT	50	2.1827873E-11	45.0	8
GGTCCAG	20	7.009689E-4	45.0	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content