##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127543
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.788204762315456	31.0	30.0	31.0	25.0	34.0
2	29.88822593164658	31.0	30.0	33.0	25.0	34.0
3	29.91626353465106	31.0	30.0	33.0	25.0	34.0
4	33.876857216781794	35.0	33.0	37.0	28.0	37.0
5	34.357040370698506	35.0	35.0	37.0	32.0	37.0
6	33.62747465560634	35.0	33.0	37.0	28.0	37.0
7	34.41783555349961	35.0	35.0	37.0	32.0	37.0
8	34.29766431713226	35.0	35.0	37.0	31.0	37.0
9	35.68543157993775	37.0	35.0	39.0	30.0	39.0
10	35.16109076938758	37.0	34.0	39.0	30.0	39.0
11	35.33355809413296	37.0	34.0	39.0	30.0	39.0
12	35.205075935174804	37.0	34.0	39.0	30.0	39.0
13	34.87610452945281	37.0	33.0	39.0	27.0	39.0
14	35.37200787185498	38.0	33.0	40.0	27.0	41.0
15	35.832205609088696	38.0	34.0	40.0	29.0	41.0
16	36.1615690394612	38.0	34.0	40.0	30.0	41.0
17	35.98745521118368	38.0	34.0	40.0	30.0	41.0
18	36.05641234720839	38.0	34.0	40.0	30.0	41.0
19	36.060450201108644	38.0	34.0	40.0	30.0	41.0
20	36.03089154246019	38.0	34.0	40.0	30.0	41.0
21	35.729589236571194	38.0	34.0	40.0	29.0	41.0
22	35.96145613636185	38.0	34.0	40.0	30.0	41.0
23	36.05061822287385	38.0	34.0	40.0	30.0	41.0
24	35.95976258987165	38.0	34.0	40.0	30.0	41.0
25	35.49536234838447	37.0	34.0	40.0	29.0	41.0
26	35.57183851720596	37.0	34.0	40.0	29.0	41.0
27	35.57516288624229	37.0	34.0	40.0	29.0	41.0
28	35.473408967955905	37.0	34.0	40.0	29.0	41.0
29	35.53654061767404	37.0	34.0	40.0	29.0	41.0
30	35.17674823392895	37.0	34.0	40.0	27.0	41.0
31	35.198207663297865	37.0	34.0	40.0	28.0	41.0
32	35.17518797581992	37.0	34.0	40.0	28.0	41.0
33	35.18657237167073	37.0	34.0	40.0	28.0	41.0
34	35.16712010851242	37.0	34.0	40.0	27.0	41.0
35	35.27070086167018	37.0	34.0	40.0	28.0	41.0
36	35.12398955646331	37.0	33.0	40.0	27.0	41.0
37	35.03311040198208	37.0	33.0	40.0	27.0	41.0
38	34.93913425276181	37.0	33.0	40.0	27.0	41.0
39	34.81502708890335	37.0	33.0	40.0	27.0	41.0
40	34.79456340214673	37.0	33.0	40.0	26.0	41.0
41	34.62665140384027	37.0	33.0	40.0	26.0	41.0
42	34.998988576401686	37.0	33.0	40.0	27.0	41.0
43	35.00432011164862	37.0	33.0	40.0	27.0	41.0
44	34.93639792070125	37.0	33.0	40.0	27.0	41.0
45	34.99033267211842	37.0	33.0	40.0	27.0	41.0
46	34.84975263244553	37.0	33.0	40.0	27.0	41.0
47	34.669264483350716	37.0	33.0	40.0	26.0	41.0
48	34.752938224755574	36.0	33.0	40.0	27.0	41.0
49	34.83844664152482	36.0	33.0	40.0	27.0	41.0
50	34.61203672486926	36.0	33.0	40.0	26.0	41.0
51	33.55690237802153	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	3.0
13	2.0
14	5.0
15	5.0
16	12.0
17	25.0
18	51.0
19	94.0
20	159.0
21	303.0
22	491.0
23	692.0
24	966.0
25	1223.0
26	1615.0
27	2081.0
28	2692.0
29	3471.0
30	4456.0
31	5827.0
32	7674.0
33	9751.0
34	13506.0
35	13268.0
36	14722.0
37	17651.0
38	19218.0
39	7570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.128442956493103	3.8810440400492383	53.58663352751621	16.403879475941448
2	18.357730334083406	8.235653857914587	51.90249562892515	21.50412017907686
3	19.737657103878693	8.357181499572693	52.49053260469019	19.414628791858433
4	19.48597727825126	3.9147581599931	49.01484205326831	27.584422508487332
5	20.19005355056726	4.998314294002807	50.19091600479838	24.62071615063155
6	19.65690002587363	9.846875171510785	51.69942685996095	18.796797942654635
7	78.4629497502803	1.775871666810409	14.744831154983027	5.016347427926268
8	78.5452749268874	5.574590530252542	8.509287063970582	7.3708474788894724
9	72.23446210297703	5.799612679645296	11.506707541770227	10.459217675607443
10	39.732482378491966	26.662380530487756	18.538845722619037	15.066291368401243
11	32.69799204974009	26.36757799330422	24.56896889676423	16.365461060191464
12	29.254447519660037	19.625538053832827	31.416071442572306	19.70394298393483
13	23.775511004131943	28.733054734481705	29.891879601389338	17.599554659997022
14	16.76611025301271	35.428835765192915	28.641320966262356	19.163733015532017
15	13.55699646393765	25.915965595916674	41.57499823588907	18.952039704256602
16	14.753455697294246	24.293767592106192	36.59314897720768	24.359627733391875
17	15.50771112487553	24.29298354280517	29.762511466721026	30.436793865598265
18	18.679190547501626	23.02360772445371	32.24794775095458	26.049253977090082
19	21.466485812627898	25.647036685666798	29.542977662435415	23.343499839269892
20	26.299365704115473	24.641885481759093	30.472860133445188	18.58588868068024
21	22.059227084199055	28.350438675583923	28.733054734481705	20.85727950573532
22	17.41295092635425	25.945759469355433	29.82366731220059	26.817622292089727
23	18.610194209011862	28.052499941196302	28.04701159608916	25.290294253702672
24	21.043099190077072	23.691617728922797	30.6375104866594	24.627772594340733
25	16.407799722446548	27.63852191025772	29.24582297734882	26.70785538994692
26	16.720635393553547	28.55585959245117	29.515535936899713	25.207969077095566
27	21.439044087092196	29.61040590232314	27.40409116925272	21.54645884133194
28	17.218506699701276	27.263746344370134	33.570638921775405	21.94710803415319
29	17.410598778451188	26.37933873281952	30.918984185725602	25.291078303003694
30	23.26352681056585	25.959088307472772	28.551155296645053	22.22622958531632
31	23.906447237402286	27.50993782489043	25.834424468610585	22.749190469096696
32	24.11735649937668	30.811569431485854	24.191057133672565	20.8800169354649
33	23.338011494162753	27.970958813890217	25.574904149972948	23.11612554197408
34	19.8827062245674	25.55216672024337	29.98518146821072	24.57994558697851
35	19.36209748869009	24.887292912978367	28.278306139890073	27.472303458441466
36	25.683887002814735	25.172686858549664	27.081062857232464	22.062363281403137
37	22.91932916741805	27.111640779972245	30.141207279113715	19.827822773496
38	22.515543777392722	29.362646323200803	25.146813231616004	22.97499666779047
39	21.434339791286074	27.62284092423732	29.62922308554762	21.313596198928987
40	24.0671773441114	22.58375606658147	30.203147173894294	23.14591941541284
41	17.648949765961284	25.007252456034436	29.98518146821072	27.35861630979356
42	22.011400076836832	23.486980861356564	29.49358255647115	25.008036505335458
43	25.033910132269117	25.847753306727927	25.313815732733275	23.80452082826968
44	22.358733917188715	25.627435453141295	27.06459782191104	24.949232807758953
45	21.613103031918644	24.639533333856033	25.62116305873313	28.126200575492188
46	25.142892985110905	25.34831390197816	28.563700085461374	20.945093027449566
47	18.38752420752217	24.499972558274465	34.29902072242302	22.813482511780343
48	20.617360419623186	23.854699983534967	31.63168500035282	23.896254596489026
49	21.376320143010595	21.934563245336864	34.03401205867825	22.655104552974294
50	21.423363101071796	22.595516806096768	30.039280869981106	25.941839222850334
51	19.275068016276865	23.11847768987714	27.002657927130457	30.603796366715542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	61.5
2	80.0
3	139.0
4	198.0
5	151.0
6	104.0
7	130.5
8	157.0
9	194.0
10	231.0
11	294.0
12	357.0
13	384.0
14	411.0
15	428.5
16	446.0
17	377.5
18	309.0
19	316.5
20	324.0
21	352.0
22	380.0
23	390.5
24	401.0
25	587.5
26	897.5
27	1021.0
28	1203.5
29	1386.0
30	1608.0
31	1830.0
32	2137.5
33	2445.0
34	2502.5
35	2560.0
36	2942.5
37	3325.0
38	3741.0
39	4157.0
40	5269.5
41	6382.0
42	7579.5
43	8777.0
44	10140.0
45	11503.0
46	14773.5
47	18044.0
48	17798.0
49	17552.0
50	16208.5
51	14865.0
52	12083.5
53	9302.0
54	7600.5
55	5899.0
56	5197.5
57	4496.0
58	3881.5
59	3267.0
60	2861.5
61	2456.0
62	2076.0
63	1696.0
64	1346.0
65	996.0
66	759.5
67	523.0
68	425.5
69	328.0
70	247.0
71	166.0
72	162.5
73	159.0
74	123.5
75	56.0
76	24.0
77	28.5
78	33.0
79	29.0
80	25.0
81	20.5
82	16.0
83	11.5
84	7.0
85	3.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.308531240444395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23399170845629	32.58273680249014
2	10.72650578416641	9.290984217087571
3	3.774643807592737	4.9042283778804014
4	1.937107373680685	3.355731008365806
5	1.2600249832539783	2.7284915675497676
6	0.8780346506870395	2.2815834659683403
7	0.8726035085178413	2.645382341641642
8	0.655357821749914	2.270606775754059
9	0.5919944964426019	2.3074570929020015
>10	3.955681879899343	28.11051958947179
>50	0.07241522892264243	2.1843613526418544
>100	0.02896609156905697	2.27531107156018
>500	0.009051903615330304	3.405126114330069
>1k	0.0036207614461321214	1.6574802223563816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	1111	0.8710787734332734	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1003	0.7864014489231084	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	951	0.7456308852700657	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	898	0.7040762723160032	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	850	0.6664419058670409	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	847	0.6640897579639808	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	797	0.6248872929129784	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	476	0.3732074672855429	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	270	0.211693311275413	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	256	0.20071662106113233	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	246	0.19287612805093185	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	234	0.18346753643869126	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	163	0.12780003606626783	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	150	0.11760739515300724	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	138	0.10819880354076665	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	137	0.1074147542397466	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	137	0.1074147542397466	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	133	0.10427855703566641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7762088080098477	0.0
2	0.0	0.0	0.0	3.1111076264475512	0.0
3	0.0	0.0	0.0	3.8387053777941555	0.0
4	0.0	0.0	0.0	5.191974471354759	0.0
5	0.0	0.0	0.0	7.667218114675051	0.0
6	0.0	0.0	0.0	8.712355832934774	0.0
7	0.0	0.0	0.0	9.809240805061822	0.0
8	0.0	0.0	0.0	11.531013070101848	0.0
9	0.0	0.0	0.0	12.22568075080561	0.0
10	0.0	0.0	0.0	14.33006907474342	0.0
11	0.0	0.0	0.0	16.701818210329066	0.0
12	0.0	0.0	0.0	19.978360239291845	0.0
13	0.0	0.0	0.0	20.893345773582244	0.0
14	0.0	0.0	0.0	21.221862430709642	0.0
15	0.0	0.0	0.0	21.797354617658357	0.0
16	0.0	0.0	0.0	22.98910955520883	0.0
17	0.0	0.0	0.0	24.7359713978815	0.0
18	0.0	0.0	0.0	27.0551892302988	0.0
19	0.0	0.0	0.0	28.535474310624654	0.0
20	0.0	0.0	0.0	29.716252557960846	0.0
21	0.0	0.0	0.0	31.125973201194892	0.0
22	0.0	0.0	0.0	32.41651835067389	0.0
23	0.0	0.0	0.0	33.73215307778553	0.0
24	0.0	0.0	0.0	34.645570513473885	0.0
25	0.0	0.0	0.0	35.38571305363681	0.0
26	0.0	0.0	0.0	36.1556494672385	0.0
27	0.0	0.0	0.0	36.84326070423308	0.0
28	0.0	0.0	0.0	37.505782363595024	0.0
29	0.0	0.0	0.0	38.27963902370181	0.0
30	0.0	0.0	0.0	39.156206142242226	0.0
31	0.0	0.0	0.0	40.0508063947061	0.0
32	0.0	0.0	0.0	40.782324392557804	0.0
33	0.0	0.0	0.0	41.53422767223603	0.0
34	0.0	0.0	0.0	42.28456285331222	0.0
35	0.0	0.0	0.0	43.07723669664348	0.0
36	0.0	0.0	0.0	43.826003779117634	0.0
37	0.0	0.0	0.0	44.49636593148977	0.0
38	0.0	0.0	0.0	45.13615016112213	0.0
39	0.0	0.0	0.0	45.83003379252487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCAT	20	7.009689E-4	45.0	21
CGTGGGT	20	7.009689E-4	45.0	4
CAGAAGC	20	7.009689E-4	45.0	21
GGGTGAT	20	7.009689E-4	45.0	7
CCGGGTT	25	3.8712584E-5	45.0	5
CAAACTA	20	7.009689E-4	45.0	15
ACACGCG	20	7.009689E-4	45.0	36
TCCGCGG	25	3.8712584E-5	45.0	2
ATAGGTC	20	7.009689E-4	45.0	26
GAATGTC	20	7.009689E-4	45.0	27
GGAGAAT	40	6.7338988E-9	45.0	8
GGATGCG	20	7.009689E-4	45.0	12
ATGGCAA	20	7.009689E-4	45.0	11
CTATCAA	20	7.009689E-4	45.0	13
CTTTTCG	25	3.8712584E-5	45.0	1
TTTGTCG	45	3.783498E-10	45.0	1
TAGGTCC	20	7.009689E-4	45.0	27
AGCTTAG	20	7.009689E-4	45.0	36
GGCCGAT	50	2.1827873E-11	45.0	8
GGTCCAG	20	7.009689E-4	45.0	29
>>END_MODULE