##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359476
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.17650969744851	31.0	30.0	31.0	27.0	34.0
2	30.42521892977556	31.0	30.0	33.0	27.0	34.0
3	30.47643514448809	31.0	30.0	34.0	26.0	34.0
4	34.13471553038311	35.0	33.0	37.0	30.0	37.0
5	34.538742502976554	35.0	35.0	37.0	32.0	37.0
6	34.02975442032292	35.0	35.0	37.0	30.0	37.0
7	34.50965293927828	35.0	35.0	37.0	32.0	37.0
8	34.59589513625388	35.0	35.0	37.0	32.0	37.0
9	36.00265386284481	37.0	35.0	39.0	31.0	39.0
10	35.40148438282389	37.0	34.0	39.0	30.0	39.0
11	35.56285537838409	37.0	35.0	39.0	30.0	39.0
12	35.59571431750659	37.0	35.0	39.0	30.0	39.0
13	35.59133294016847	37.0	35.0	39.0	30.0	39.0
14	36.30963680468237	38.0	35.0	40.0	30.0	41.0
15	36.598768763422314	38.0	35.0	40.0	31.0	41.0
16	36.72886924300927	38.0	35.0	40.0	31.0	41.0
17	36.49855901367546	38.0	35.0	40.0	31.0	41.0
18	36.61056092757236	38.0	35.0	40.0	31.0	41.0
19	36.6007243877199	38.0	35.0	40.0	31.0	41.0
20	36.6227703657546	38.0	35.0	40.0	31.0	41.0
21	36.3429686543747	38.0	35.0	40.0	30.0	41.0
22	36.488388654597244	38.0	35.0	40.0	30.0	41.0
23	36.584564755366145	38.0	35.0	40.0	31.0	41.0
24	36.51447384526366	38.0	35.0	40.0	30.0	41.0
25	36.051441542689915	38.0	34.0	40.0	30.0	41.0
26	36.18261580745307	38.0	35.0	40.0	30.0	41.0
27	36.24739621003906	38.0	35.0	40.0	30.0	41.0
28	36.14695556866105	38.0	35.0	40.0	30.0	41.0
29	36.26422348084434	38.0	35.0	40.0	30.0	41.0
30	35.81119462773593	38.0	34.0	40.0	29.0	41.0
31	35.984307714562306	38.0	34.0	40.0	30.0	41.0
32	36.02429369415483	38.0	34.0	40.0	30.0	41.0
33	36.008253680356965	38.0	34.0	40.0	30.0	41.0
34	36.00833435333652	38.0	35.0	40.0	30.0	41.0
35	36.0274594131458	38.0	35.0	40.0	30.0	41.0
36	35.803811659192824	38.0	34.0	40.0	29.0	41.0
37	35.90639152544259	38.0	34.0	40.0	30.0	41.0
38	35.79756367601731	38.0	34.0	40.0	29.0	41.0
39	35.7135163404511	38.0	34.0	40.0	29.0	41.0
40	35.65244411309796	38.0	34.0	40.0	29.0	41.0
41	35.559091566613624	38.0	34.0	40.0	29.0	41.0
42	35.80334987593052	38.0	34.0	40.0	29.0	41.0
43	35.78298968498592	38.0	34.0	40.0	29.0	41.0
44	35.737743270760774	38.0	34.0	40.0	29.0	41.0
45	35.79125171082353	38.0	34.0	40.0	29.0	41.0
46	35.669841658413915	38.0	34.0	40.0	29.0	41.0
47	35.61384348329235	38.0	34.0	40.0	29.0	41.0
48	35.611453894001265	38.0	34.0	40.0	29.0	41.0
49	35.63330792598115	37.0	34.0	40.0	29.0	41.0
50	35.45172695812794	37.0	34.0	40.0	29.0	41.0
51	34.33935506125583	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	9.0
12	10.0
13	9.0
14	6.0
15	24.0
16	25.0
17	59.0
18	99.0
19	220.0
20	404.0
21	695.0
22	1059.0
23	1451.0
24	1995.0
25	2648.0
26	3534.0
27	4629.0
28	5999.0
29	7577.0
30	10110.0
31	13036.0
32	16984.0
33	23023.0
34	32333.0
35	35146.0
36	42119.0
37	54878.0
38	67648.0
39	33743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.05355573111974	4.878767984510788	55.23512000801166	16.83255627635781
2	19.346215046345236	5.283801978435278	54.1713494085836	21.19863356663588
3	21.477372620147104	5.237345469516741	52.4911259722485	20.79415593808766
4	20.729895737128487	4.808387764412645	48.44662786945443	26.015088629004442
5	19.043552281654406	6.065217149406358	49.44419098910637	25.447039579832868
6	22.090487264796536	7.624431116402763	51.969255249307324	18.315826369493372
7	83.42281543135007	2.427422136665591	9.63930832656422	4.510454105420111
8	85.90253591338504	4.018627112797516	5.824032758793355	4.25480421502409
9	80.34889672745885	5.449877043251845	7.821106276914175	6.380119952375124
10	40.81885856079405	28.869799374645318	15.686721783929942	14.624620280630696
11	29.445915721772803	25.73996595043897	25.48960153111752	19.32451679667071
12	26.137767194471955	21.684340540119507	31.04240616897929	21.135486096429247
13	24.329023356218496	24.129566368825735	31.354248962378573	20.187161312577196
14	19.644426888025905	27.45663131891976	31.876676050696013	21.02226574235832
15	18.479954155492994	25.073996595043894	35.96178882595778	20.484260423505322
16	20.88790350398914	24.495376603723198	32.038856557878695	22.577863334408974
17	21.20364085502231	24.19493930053745	30.562819214634633	24.038600629805607
18	22.540586854198892	23.033526577574023	30.967575025870993	23.458311542356096
19	24.31817423138123	24.153211897317206	28.830575615618287	22.698038255683272
20	26.412333507661153	25.17608964158942	29.34076266565779	19.070814185091635
21	25.587800020029157	25.834269881716722	28.355439584283793	20.222490513970335
22	23.104463163048436	24.349330692452348	29.82007143731431	22.726134707184904
23	22.138612869843886	26.124692608129614	28.92793955646552	22.808754965560986
24	22.957304521025048	24.523473055224827	29.652605459057074	22.866616964693055
25	20.454494875874886	27.13644304487643	29.283178849213854	23.125883230034827
26	20.51207869231882	26.984833479842884	30.021753886212153	22.481333941626144
27	20.781081351745318	27.21489056293049	29.78168222635169	22.222345858972503
28	19.498380976755055	26.72918358944686	31.635213477394874	22.13722195640321
29	20.59247348918982	24.657000745529604	31.856368714462164	22.894157050818414
30	21.097096885466623	25.671254826469642	31.65357353481178	21.578074753251954
31	23.365954889895292	26.489390112274535	27.82800520757992	22.316649790250253
32	23.490858916867886	26.91417507705661	27.229077880025372	22.36588812605014
33	22.984288241774138	27.133383035306945	27.570964403743226	22.311364319175688
34	21.552203763255406	26.580634033982797	28.73070803057784	23.136454172183957
35	20.88178348485017	25.655398413245944	29.72882751560605	23.733990586297836
36	22.169769330915	26.498013775606715	30.568939233773605	20.76327765970468
37	22.19397122478274	26.545304832589657	29.86152065784642	21.399203284781183
38	22.73336745707641	26.13748901178382	27.253279773893112	23.87586375724666
39	22.27492238703001	26.719169012674005	29.00110160344501	22.004806996850974
40	21.248706450500173	25.93970112051987	29.595856190677544	23.215736238302416
41	18.90334820683439	26.871891308460093	28.95603600796715	25.268724476738363
42	20.61723174843383	25.776129699896515	28.665613281554265	24.94102527011539
43	21.706038789794032	26.36504245067821	28.759917212832008	23.169001546695746
44	21.674604146034785	24.825857637227518	29.353559069311995	24.1459791474257
45	21.427021553594678	24.54378039145868	28.309261258053386	25.719936796893254
46	22.055436246091535	25.419221311019374	30.01146112675116	22.513881316137933
47	19.494486419121166	26.381177046590036	31.599606093313604	22.524730440975198
48	20.604991710155893	25.296542745551857	30.60927572355317	23.489189820739075
49	21.529670965516473	23.72926148059954	31.78153757135386	22.959529982530128
50	21.084856847188686	25.02976554763044	29.659003660884174	24.226373944296697
51	20.170748533977235	25.131024046111563	28.322057661707596	26.376169758203606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	205.0
2	346.0
3	528.0
4	710.0
5	560.0
6	410.0
7	393.0
8	376.0
9	438.0
10	500.0
11	502.0
12	504.0
13	545.0
14	586.0
15	577.0
16	568.0
17	658.0
18	748.0
19	837.5
20	927.0
21	1228.0
22	1529.0
23	1711.0
24	1893.0
25	2277.5
26	3344.0
27	4026.0
28	4417.5
29	4809.0
30	5656.5
31	6504.0
32	7995.0
33	9486.0
34	10308.0
35	11130.0
36	12078.5
37	13027.0
38	14747.5
39	16468.0
40	18254.5
41	20041.0
42	22282.0
43	24523.0
44	28706.0
45	32889.0
46	34353.5
47	35818.0
48	34316.5
49	32815.0
50	29880.0
51	26945.0
52	24209.5
53	21474.0
54	19726.0
55	17978.0
56	17018.0
57	16058.0
58	15934.5
59	15811.0
60	13847.0
61	11883.0
62	10458.0
63	9033.0
64	7833.0
65	6633.0
66	5142.0
67	3651.0
68	2987.5
69	2324.0
70	2061.5
71	1799.0
72	1454.0
73	1109.0
74	904.5
75	530.5
76	361.0
77	285.5
78	210.0
79	141.5
80	73.0
81	61.0
82	49.0
83	30.5
84	12.0
85	12.0
86	12.0
87	7.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.67310667321556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55205316415301	25.50278185394699
2	9.869766135325547	6.844309080996662
3	3.4629219612988393	3.6021078769540638
4	1.6706458469644394	2.317059266598502
5	1.1198628413253107	1.9414561878321377
6	0.8778940333052371	1.8263588078743158
7	0.7494528526443219	1.8190101094397586
8	0.6503646537257021	1.804013041209613
9	0.6300596168410381	1.9661511874693167
>10	7.295189432429561	46.73196966019005
>50	0.08525262339088864	1.95995106185106
>100	0.030853330370035893	1.8490316825966333
>500	0.004059648732899459	1.1804186731314987
>1k	0.0016238594931597837	0.6553815099094036
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1243	0.3457810813517453	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGC	1085	0.3018282166264229	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCG	992	0.2759572266298724	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	925	0.2573189865248306	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	793	0.2205988716910169	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	775	0.2155915833045878	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	708	0.19695334319954602	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGGTACAT	423	0.11767127708108469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27901723619935687	0.0
2	0.0	0.0	0.0	1.1313689926448498	0.0
3	0.0	0.0	0.0	1.4395954110983766	0.0
4	0.0	0.0	0.0	1.9497824611378785	0.0
5	0.0	0.0	0.0	2.9003327064950093	0.0
6	0.0	0.0	0.0	3.3095394407415237	0.0
7	0.0	0.0	0.0	3.788570029709911	0.0
8	0.0	0.0	0.0	4.51879958606416	0.0
9	0.0	0.0	0.0	4.801433197209271	0.0
10	0.0	0.0	0.0	5.671032280319131	0.0
11	0.0	0.0	0.0	6.829384993713071	0.0
12	0.0	0.0	0.0	8.34158608641467	0.0
13	0.0	0.0	0.0	8.736883686254437	0.0
14	0.0	0.0	0.0	8.884042328277827	0.0
15	0.0	0.0	0.0	9.15054134351111	0.0
16	0.0	0.0	0.0	9.662675672367557	0.0
17	0.0	0.0	0.0	10.466901823765703	0.0
18	0.0	0.0	0.0	11.369882829451758	0.0
19	0.0	0.0	0.0	12.084812337958585	0.0
20	0.0	0.0	0.0	12.827838297967041	0.0
21	0.0	0.0	0.0	13.620102593775384	0.0
22	0.0	0.0	0.0	14.51863267645128	0.0
23	0.0	0.0	0.0	15.335377048815499	0.0
24	0.0	0.0	0.0	15.972415404644538	0.0
25	0.0	0.0	0.0	16.54102081919238	0.0
26	0.0	0.0	0.0	17.069011561272518	0.0
27	0.0	0.0	0.0	17.606738697437383	0.0
28	0.0	0.0	0.0	18.215124236388522	0.0
29	0.0	0.0	0.0	18.809878823621048	0.0
30	0.0	0.0	0.0	19.485306390412713	0.0
31	0.0	0.0	0.0	20.10871379452314	0.0
32	0.0	0.0	0.0	20.710701131647177	0.0
33	0.0	0.0	0.0	21.327432151242363	0.0
34	0.0	0.0	0.0	21.907721238691874	0.0
35	0.0	0.0	0.0	22.549488700219207	0.0
36	0.0	0.0	0.0	23.19403798862789	0.0
37	0.0	0.0	0.0	23.816610844673914	0.0
38	0.0	0.0	0.0	24.447529181363986	0.0
39	0.0	0.0	0.0	25.080672979559136	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGAACT	25	3.8854378E-5	45.000004	12
TCGACGC	25	3.8854378E-5	45.000004	14
CGTAGGC	20	7.0268114E-4	45.0	33
CGGTGAT	20	7.0268114E-4	45.0	31
TACCGGG	30	2.161325E-6	44.999996	3
CTTTGCG	145	0.0	41.89655	1
GACCGAT	150	0.0	40.5	9
CGTTTGG	190	0.0	40.263157	2
GTTCGCG	45	1.9219442E-8	40.0	1
CGGGTAC	40	3.449386E-7	39.375	6
GCGATCT	115	0.0	39.130436	9
CGATGAA	255	0.0	38.82353	19
CGCGGGC	70	0.0	38.57143	4
GCGGTCT	35	6.2363124E-6	38.57143	36
CGCATGG	35	6.2363124E-6	38.57143	2
GGTATCC	35	6.2363124E-6	38.57143	8
TCCGTTG	35	6.2363124E-6	38.57143	1
CGAGATA	70	0.0	38.57143	19
TTTCGCG	65	9.094947E-12	38.07692	1
TTACACG	65	9.094947E-12	38.07692	34
>>END_MODULE