##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1017850
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.13907844967333	31.0	30.0	31.0	26.0	34.0
2	30.52940315370634	31.0	30.0	33.0	27.0	34.0
3	30.54255342142752	31.0	30.0	34.0	27.0	34.0
4	34.15935255686005	35.0	33.0	37.0	30.0	37.0
5	34.485083263742204	35.0	35.0	37.0	32.0	37.0
6	34.07207643562411	35.0	35.0	37.0	30.0	37.0
7	34.52784987964828	35.0	35.0	37.0	32.0	37.0
8	34.72907599351574	35.0	35.0	37.0	32.0	37.0
9	36.03062435525863	38.0	35.0	39.0	31.0	39.0
10	35.399351574397016	37.0	34.0	39.0	30.0	39.0
11	35.5345021368571	37.0	35.0	39.0	30.0	39.0
12	35.48173011740433	37.0	35.0	39.0	30.0	39.0
13	35.45287812546053	37.0	35.0	39.0	30.0	39.0
14	35.87213145355406	38.0	34.0	40.0	27.0	41.0
15	36.18702657562509	38.0	35.0	40.0	29.0	41.0
16	36.385958638306235	38.0	35.0	40.0	30.0	41.0
17	36.20451638257111	38.0	35.0	40.0	30.0	41.0
18	36.356281377413175	38.0	35.0	40.0	30.0	41.0
19	36.37059291644152	38.0	35.0	40.0	30.0	41.0
20	36.385216878714935	38.0	35.0	40.0	30.0	41.0
21	36.068568060126736	38.0	34.0	40.0	30.0	41.0
22	36.33479294591541	38.0	34.0	40.0	30.0	41.0
23	36.47000049123152	38.0	35.0	40.0	31.0	41.0
24	36.38741759591296	38.0	35.0	40.0	30.0	41.0
25	35.90146288745886	38.0	34.0	40.0	29.0	41.0
26	36.011518396620325	38.0	34.0	40.0	30.0	41.0
27	36.09364444662769	38.0	34.0	40.0	30.0	41.0
28	35.9945895760672	38.0	34.0	40.0	30.0	41.0
29	36.092079383013214	38.0	34.0	40.0	30.0	41.0
30	35.611258043916095	38.0	34.0	40.0	29.0	41.0
31	35.68767107137594	38.0	34.0	40.0	29.0	41.0
32	35.77147418578376	38.0	34.0	40.0	29.0	41.0
33	35.79788475708602	38.0	34.0	40.0	29.0	41.0
34	35.69860195510144	38.0	34.0	40.0	29.0	41.0
35	35.725926217026085	38.0	34.0	40.0	29.0	41.0
36	35.51617625386845	38.0	34.0	40.0	28.0	41.0
37	35.562936582011105	38.0	34.0	40.0	29.0	41.0
38	35.45869921894189	38.0	34.0	40.0	29.0	41.0
39	35.41090337476052	38.0	34.0	40.0	28.0	41.0
40	35.28248464901508	38.0	34.0	40.0	27.0	41.0
41	35.15143488726237	37.0	34.0	40.0	27.0	41.0
42	35.42519821191728	38.0	34.0	40.0	28.0	41.0
43	35.398933045144176	38.0	34.0	40.0	28.0	41.0
44	35.35086014638699	37.0	34.0	40.0	28.0	41.0
45	35.45470354177924	37.0	34.0	40.0	29.0	41.0
46	35.40252100014737	37.0	34.0	40.0	29.0	41.0
47	35.371921206464606	37.0	34.0	40.0	29.0	41.0
48	35.321099376135976	37.0	34.0	40.0	28.0	41.0
49	35.32201011936926	37.0	34.0	40.0	28.0	41.0
50	35.1336729380557	37.0	34.0	40.0	28.0	41.0
51	33.9972382964091	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	18.0
12	11.0
13	21.0
14	30.0
15	57.0
16	130.0
17	271.0
18	461.0
19	841.0
20	1342.0
21	2144.0
22	3193.0
23	4723.0
24	6598.0
25	8937.0
26	11765.0
27	15178.0
28	19058.0
29	24561.0
30	31075.0
31	40074.0
32	52163.0
33	69398.0
34	91111.0
35	92404.0
36	116033.0
37	150729.0
38	183282.0
39	92232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.899985263054475	4.2756791275728245	55.16136955347055	16.662966055902146
2	21.020386107972687	3.561133762342192	54.18863290268704	21.229847226998082
3	22.545365230633195	3.5829444417153806	52.4023186127622	21.469371714889228
4	20.672692439946946	4.681436360956919	47.119614874490345	27.526256324605786
5	18.57424964385715	5.623913150267721	50.54693717148893	25.254900034386207
6	23.101537554649507	6.07633737780616	52.62533772166822	18.19678734587611
7	84.43758903571253	2.286780959866385	9.101832293559955	4.173797710861129
8	85.97976126148254	2.6805521442255733	7.061354816525029	4.278331777766862
9	80.24571400501056	5.391462396227342	9.243994694699612	5.118828904062485
10	39.14407820405757	30.644790489757824	16.70756987768335	13.503561428501254
11	30.010807093383114	24.253475462985705	27.116274500171933	18.61944294345925
12	27.769415925725795	21.725696320675937	30.548607358648134	19.95628039495014
13	26.364297293314337	22.00383160583583	31.810777619492065	19.821093481357764
14	20.65549933683745	24.509603576165446	34.64537996757872	20.189517119418383
15	19.437638158864274	24.56285307265314	36.13037284472172	19.86913592376087
16	25.433904799331923	24.271945768040478	30.328240899936137	19.965908532691458
17	25.10075158422164	24.062877634229014	28.94837156751977	21.88799921402957
18	25.227882300928428	22.770840497126297	31.39833963747114	20.602937564474136
19	25.31266886083411	24.88578867220121	28.91221692783809	20.88932553912659
20	24.336100604214767	29.179938104828807	28.564621506115834	17.919339784840595
21	24.72839809402171	25.831900574740875	31.352065628530728	18.087635702706688
22	22.71611730608636	22.935992533280935	32.55617232401631	21.7917178366164
23	21.10576214569927	24.55273370339441	32.37628334233925	21.965220808567075
24	21.85980252493	26.595962076926856	29.777177383700938	21.767058014442206
25	23.248710517266787	26.123004371960505	27.593948027705455	23.03433708306725
26	20.940118878027214	26.58387778159847	28.19403644937859	24.281966890995726
27	20.977354227047208	24.944343469076976	31.859507786019552	22.218794517856267
28	18.75531758117601	25.901851942820652	30.65441862749914	24.6884118485042
29	21.12177629316697	26.77820896988751	29.437932897774722	22.6620818391708
30	23.718426094218206	24.595863830623372	30.53465638355357	21.151053691604854
31	22.396718573463673	26.715724320872425	30.0	20.8875571056639
32	24.202387385174635	26.85464459399715	27.575281230043718	21.367686790784497
33	24.47767352753353	25.737584123397355	27.75330353195461	22.031438817114505
34	20.10630250036842	26.120155229159504	31.805865304317926	21.967676966154148
35	20.262907108120057	28.167313454831262	29.111656923908235	22.458122513140445
36	23.742005207054085	27.949894385223757	27.638158864272732	20.669941543449426
37	21.37819914525716	28.721913837991845	28.3026968610306	21.59719015572039
38	21.70329616348185	27.729036694994353	28.06277938792553	22.504887753598272
39	21.192317139067644	26.456157587070788	27.45954708454094	24.891978189320625
40	22.53819325047895	26.567765387827286	28.752075453161076	22.14196590853269
41	20.166330991796432	26.989438522375597	27.628923711745344	25.215306774082624
42	21.27130716706784	25.379476347202434	30.2191875030702	23.13002898265953
43	21.434297784545855	24.10453406690573	30.90877830721619	23.552389841332218
44	21.637078154934418	24.35005157930933	29.142113277987914	24.870756987768335
45	20.669450311932014	24.362332367244683	28.015424669646805	26.952792651176498
46	20.739696418922236	25.17011347448052	29.585302352998973	24.50488775359827
47	18.350837549737193	27.16362921845066	31.588937466227833	22.896595765584323
48	19.18318023284374	28.00589477820897	30.193446971557698	22.617478017389594
49	22.47433315321511	25.055263545709096	30.051874048238936	22.418529252836862
50	20.191678538095005	25.868742938546934	28.36400255440389	25.57557596895417
51	19.475855970919095	26.390529056344253	27.31060568846097	26.823009284275678
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	222.0
1	521.0
2	820.0
3	2564.5
4	4309.0
5	2959.5
6	1610.0
7	1655.0
8	1700.0
9	1845.0
10	1990.0
11	2076.5
12	2163.0
13	2223.5
14	2284.0
15	2177.0
16	2070.0
17	2220.5
18	2371.0
19	2527.5
20	2684.0
21	3345.0
22	4006.0
23	4457.5
24	4909.0
25	6218.5
26	8981.5
27	10435.0
28	12696.0
29	14957.0
30	17361.0
31	19765.0
32	22752.0
33	25739.0
34	28773.5
35	31808.0
36	35081.5
37	38355.0
38	40777.0
39	43199.0
40	47730.0
41	52261.0
42	57054.0
43	61847.0
44	70457.0
45	79067.0
46	88589.0
47	98111.0
48	99244.5
49	100378.0
50	90609.5
51	80841.0
52	73424.0
53	66007.0
54	61556.0
55	57105.0
56	54620.5
57	52136.0
58	47820.0
59	43504.0
60	37827.0
61	32150.0
62	27636.0
63	23122.0
64	20275.0
65	17428.0
66	14396.0
67	11364.0
68	9200.0
69	7036.0
70	6015.0
71	4994.0
72	4086.5
73	3179.0
74	2782.5
75	1809.5
76	1233.0
77	838.0
78	443.0
79	307.5
80	172.0
81	131.5
82	91.0
83	72.0
84	53.0
85	34.0
86	15.0
87	8.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1017850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.20026770171243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01514034978486	25.701524865905316
2	10.1175081393062	7.122779899556653
3	3.5951697196753165	3.796528096969847
4	1.9087379690287936	2.6875234992894574
5	1.2418481961027488	2.185669447385272
6	1.010622492798381	2.134450937512497
7	0.8055753210003238	1.9849526867173024
8	0.7311643687406209	2.058974521089875
9	0.6768422384297939	2.1442525186099513
>10	6.741582675661981	42.55494002433649
>50	0.10481708663901604	2.5030310362038053
>100	0.04645887013702633	2.925308404552344
>500	0.002266286347519527	0.5165359126362169
>1k	0.002266286347519527	1.6835281492349905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	3078	0.3024021221201552	No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCT	2700	0.26526501940364494	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2598	0.2552438964483961	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1981	0.19462592719948912	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1929	0.18951711941838187	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC	1712	0.16819767156260745	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCC	1704	0.1674117011347448	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1181	0.11602888441322395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2947389104484944	0.0
2	0.0	0.0	0.0	1.0497617527140541	0.0
3	0.0	0.0	0.0	1.4039396767696615	0.0
4	0.0	0.0	0.0	1.9294591540993271	0.0
5	0.0	0.0	0.0	2.830476003340374	0.0
6	0.0	0.0	0.0	3.430564425013509	0.0
7	0.0	0.0	0.0	4.008940413616938	0.0
8	0.0	0.0	0.0	4.875276317728545	0.0
9	0.0	0.0	0.0	5.30048631920224	0.0
10	0.0	0.0	0.0	6.101095446283834	0.0
11	0.0	0.0	0.0	7.569877683352164	0.0
12	0.0	0.0	0.0	8.832146190499582	0.0
13	0.0	0.0	0.0	9.27582649702805	0.0
14	0.0	0.0	0.0	9.470354177924056	0.0
15	0.0	0.0	0.0	9.726777030014246	0.0
16	0.0	0.0	0.0	10.26231763029916	0.0
17	0.0	0.0	0.0	11.008400058947782	0.0
18	0.0	0.0	0.0	11.842511175517021	0.0
19	0.0	0.0	0.0	12.43473989291153	0.0
20	0.0	0.0	0.0	13.118632411455518	0.0
21	0.0	0.0	0.0	13.97190155720391	0.0
22	0.0	0.0	0.0	14.906322149629121	0.0
23	0.0	0.0	0.0	15.774131748292971	0.0
24	0.0	0.0	0.0	16.455666355553372	0.0
25	0.0	0.0	0.0	17.095937515350986	0.0
26	0.0	0.0	0.0	17.67804686348676	0.0
27	0.0	0.0	0.0	18.32480227931424	0.0
28	0.0	0.0	0.0	18.95632951810188	0.0
29	0.0	0.0	0.0	19.679324065432038	0.0
30	0.0	0.0	0.0	20.560986392886967	0.0
31	0.0	0.0	0.0	21.262563246057866	0.0
32	0.0	0.0	0.0	21.90951515449231	0.0
33	0.0	0.0	0.0	22.559217959424277	0.0
34	0.0	0.0	0.0	23.30146878223707	0.0
35	0.0	0.0	0.0	24.14294837156752	0.0
36	0.0	0.0	0.0	24.844819963648867	0.0
37	0.0	0.0	0.0	25.545512600088422	0.0
38	0.0	0.0	0.0	26.413027459841825	0.0
39	0.0	0.0	0.0	27.904701085621653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAT	25	3.8904895E-5	45.000004	44
TTGCCCG	110	0.0	40.909092	1
CGTTTTT	1635	0.0	39.63303	1
CGTTTGG	670	0.0	39.626865	2
TTGGGAC	2090	0.0	39.07895	5
CGTATAA	35	6.247583E-6	38.57143	28
CTTTGCG	280	0.0	38.57143	1
GGCGATT	835	0.0	38.532936	8
TTTGGGA	5770	0.0	38.526863	4
TTGGGAT	3690	0.0	37.92683	5
TTTGGGC	1875	0.0	37.92	4
TTTCGCG	305	0.0	37.622948	1
GGTCCGT	30	1.1398299E-4	37.500004	10
TGGGCGA	1280	0.0	37.44141	6
GGGCGAT	1920	0.0	37.382816	7
CTTGGGA	3010	0.0	37.375416	4
TTGTTCG	250	0.0	36.899998	1
ACGGGAT	110	0.0	36.81818	5
TTACACG	515	0.0	36.699028	34
TGGGATG	1590	0.0	36.650944	6
>>END_MODULE