##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934048.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449040
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.975841795831105	31.0	30.0	31.0	26.0	34.0
2	30.071579369321217	31.0	30.0	33.0	25.0	34.0
3	30.143559593800106	31.0	30.0	34.0	25.0	34.0
4	33.93114644575093	35.0	33.0	37.0	28.0	37.0
5	34.387281756636376	35.0	35.0	37.0	32.0	37.0
6	33.75936887582398	35.0	35.0	37.0	28.0	37.0
7	34.332322287546766	35.0	35.0	37.0	32.0	37.0
8	34.205048548013536	35.0	35.0	37.0	30.0	37.0
9	35.725258328879384	37.0	35.0	39.0	30.0	39.0
10	35.25446730803492	37.0	34.0	39.0	30.0	39.0
11	35.39197844290041	37.0	35.0	39.0	30.0	39.0
12	35.357805540709066	37.0	35.0	39.0	30.0	39.0
13	35.29717619811153	37.0	34.0	39.0	30.0	39.0
14	36.055409317655446	38.0	34.0	40.0	29.0	41.0
15	36.314949670407984	38.0	35.0	40.0	30.0	41.0
16	36.49621414573312	38.0	35.0	40.0	31.0	41.0
17	36.2197844290041	38.0	34.0	40.0	30.0	41.0
18	36.335829770176375	38.0	35.0	40.0	30.0	41.0
19	36.33756458221985	38.0	35.0	40.0	30.0	41.0
20	36.30675663637983	38.0	34.0	40.0	30.0	41.0
21	36.062201585604846	38.0	34.0	40.0	30.0	41.0
22	36.230320238731515	38.0	34.0	40.0	30.0	41.0
23	36.23097496882237	38.0	34.0	40.0	30.0	41.0
24	36.15719757705327	38.0	34.0	40.0	30.0	41.0
25	35.771960181721006	38.0	34.0	40.0	29.0	41.0
26	35.84601149118119	38.0	34.0	40.0	29.0	41.0
27	35.79955905932656	38.0	34.0	40.0	29.0	41.0
28	35.675167022982365	38.0	34.0	40.0	29.0	41.0
29	35.81661990023161	38.0	34.0	40.0	29.0	41.0
30	35.37660342063068	38.0	34.0	40.0	27.0	41.0
31	35.57787502226973	38.0	34.0	40.0	29.0	41.0
32	35.551692499554605	38.0	34.0	40.0	29.0	41.0
33	35.52793515054338	38.0	34.0	40.0	28.0	41.0
34	35.494085159451274	38.0	34.0	40.0	28.0	41.0
35	35.45453189025476	38.0	34.0	40.0	28.0	41.0
36	35.22252137894174	38.0	34.0	40.0	27.0	41.0
37	35.2901701407447	38.0	34.0	40.0	27.0	41.0
38	35.220140744699805	38.0	34.0	40.0	27.0	41.0
39	35.151349545697485	37.0	34.0	40.0	27.0	41.0
40	35.10760956707643	37.0	34.0	40.0	27.0	41.0
41	35.00887226082309	37.0	33.0	40.0	26.0	41.0
42	35.1597162836273	37.0	34.0	40.0	27.0	41.0
43	35.118216639942986	37.0	34.0	40.0	27.0	41.0
44	35.09557723142704	37.0	34.0	40.0	27.0	41.0
45	35.130404863709245	37.0	34.0	40.0	27.0	41.0
46	34.9955059682879	37.0	34.0	40.0	27.0	41.0
47	34.90097986816319	37.0	33.0	40.0	27.0	41.0
48	34.8980625334046	37.0	33.0	40.0	27.0	41.0
49	34.90604845893461	37.0	34.0	40.0	27.0	41.0
50	34.722084892214504	36.0	33.0	40.0	26.0	41.0
51	33.64678647781935	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	1.0
11	5.0
12	8.0
13	15.0
14	19.0
15	32.0
16	62.0
17	99.0
18	225.0
19	424.0
20	906.0
21	1524.0
22	2285.0
23	3145.0
24	3868.0
25	4707.0
26	5615.0
27	6877.0
28	8579.0
29	10960.0
30	14128.0
31	18006.0
32	22834.0
33	30157.0
34	41610.0
35	43192.0
36	51035.0
37	64681.0
38	76677.0
39	37356.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.290397292000712	4.750356315695706	52.016301443078575	17.942944949225012
2	18.350481026189204	7.897514698022448	52.98904329235703	20.76296098343132
3	21.107028327097808	7.815116693390343	50.39729200071263	20.680562978799216
4	19.503607696419028	5.106894708711919	49.173124888651344	26.21637270621771
5	21.949269552823804	5.666755745590593	47.83270978086585	24.551264920719756
6	22.356805629787992	9.814493140922858	50.08573846427935	17.7429627650098
7	81.98401033315518	2.6158026011045785	11.357340103331552	4.042846962408694
8	82.7712453233565	6.457108498129342	6.192098699447711	4.579547479066453
9	77.0557188669161	6.160030286834135	7.968555139853911	8.815695706395866
10	42.07286655977196	27.667468376982008	15.25877427400677	15.000890789239266
11	34.16800285052556	25.153215749153752	22.984589346160696	17.694192054159984
12	29.584669517192232	22.022759665063248	26.840370568323536	21.552200249420984
13	25.82264386246214	23.625734901122396	29.263317299127028	21.28830393728844
14	20.676999821842152	28.27944058435774	28.95532691965081	22.088232674149296
15	19.041510778549796	24.956128629966152	34.04462854088723	21.957732050596828
16	21.67713344022804	23.59099412079102	31.168938179226796	23.562934259754144
17	21.73213967575272	24.883306609656156	29.266657758774272	24.117895955816852
18	22.307366827008728	23.39390700160342	29.946775342953856	24.351950828433992
19	23.998752895065028	25.423792980580796	27.668136468911452	22.909317655442724
20	28.4814270443613	24.37310707286656	28.602797078211296	18.54266880456084
21	26.140432923570284	27.250801710315343	26.648405487261712	19.960359878852664
22	24.239488686976664	22.44722073757349	29.63633529306966	23.676955282380188
23	24.454168893639768	26.167156600748264	27.551443078567612	21.82723142704436
24	23.471851060039196	24.657491537502228	28.832175307322288	23.03848209513629
25	22.50779440584358	27.105825761624804	26.69895777659006	23.687422055941564
26	22.059282023873152	26.816987350792804	28.04159985747372	23.082130767860324
27	23.139363976483164	27.369722073757348	27.4581329057545	22.032781044004988
28	20.84558168537324	28.141590949581328	30.123597006948156	20.889230358097276
29	22.724701585604844	24.923392125423124	28.9956351327276	23.356271156244432
30	21.677578834847676	27.786834135043648	28.45871191876002	22.076875111348652
31	24.383796543737752	25.7464813825049	25.210226260466772	24.659495813290576
32	25.457420274363084	27.372394441475144	25.206440406199892	21.963744877961876
33	24.053536433279888	26.1609210760734	26.123062533404596	23.662479957242116
34	23.033582754320328	25.299973276322824	27.066185640477464	24.600258328879388
35	23.565161232852308	25.290842686620348	26.437956529485124	24.70603955104222
36	23.525743809014788	28.809682879030824	25.181498307500444	22.483075004453944
37	22.596205237840728	28.043826830571888	26.877561019062888	22.482406912524496
38	22.077320505968288	27.168403705683236	25.697487974345268	25.056787814003208
39	22.458800997683948	25.96717441653305	26.820550507749868	24.75347407803314
40	22.085337609121684	24.410742918225548	28.411500089078924	25.092419383573844
41	19.835649385355424	26.239087831818992	27.156378050953144	26.76888473187244
42	21.696508106182076	25.366782469267772	26.376714769285588	26.559994655264564
43	21.91742383752004	26.904507393550688	26.8601906288972	24.317878140032068
44	22.06908070550508	25.145866737929808	28.09103866025298	24.694013896312132
45	22.872127204703364	24.164885088188136	26.444192054159988	26.518795652948512
46	23.195038303937288	26.35578122216284	26.399875289506504	24.049305184393372
47	20.048548013539996	26.584045964724744	29.86014608943524	23.507259932300016
48	21.154462854088724	24.612506680919292	28.872928915018708	25.360101549973272
49	22.024763940851592	23.661143773383216	29.68399251737039	24.630099768394796
50	20.913281667557456	25.562756101906288	27.949180473899876	25.574781756636376
51	20.45007126313914	25.21891145554962	26.68359166221272	27.64742561909852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	89.0
1	263.5
2	438.0
3	695.0
4	952.0
5	777.5
6	603.0
7	629.0
8	655.0
9	693.0
10	731.0
11	756.5
12	782.0
13	811.0
14	840.0
15	833.5
16	827.0
17	852.0
18	877.0
19	924.5
20	972.0
21	1417.0
22	1862.0
23	1764.0
24	1666.0
25	2340.5
26	3884.0
27	4753.0
28	5356.5
29	5960.0
30	7058.0
31	8156.0
32	9601.0
33	11046.0
34	11966.5
35	12887.0
36	13458.0
37	14029.0
38	15820.5
39	17612.0
40	19274.5
41	20937.0
42	22923.0
43	24909.0
44	25584.5
45	26260.0
46	27737.5
47	29215.0
48	35666.5
49	42118.0
50	40403.5
51	38689.0
52	37544.0
53	36399.0
54	32675.0
55	28951.0
56	27436.0
57	25921.0
58	24182.0
59	22443.0
60	21063.5
61	19684.0
62	17237.5
63	14791.0
64	12544.0
65	10297.0
66	8515.0
67	6733.0
68	5734.0
69	4735.0
70	3979.5
71	3224.0
72	2684.5
73	2145.0
74	1828.5
75	1040.5
76	569.0
77	459.0
78	349.0
79	281.5
80	214.0
81	160.5
82	107.0
83	89.5
84	72.0
85	42.0
86	12.0
87	7.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.85341800412399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.50493689929273	24.477418353233453
2	8.712300595036753	5.724577064526414
3	3.003135772867033	2.9598982460741583
4	1.613245037890243	2.12002454291548
5	1.0741169055320323	1.764420584137001
6	0.9077395574211408	1.7893408271301152
7	0.7763553104023826	1.7854147872659636
8	0.70901941030588	1.8634968847853262
9	0.6168053008288541	1.8237746137760809
>10	7.934185717321227	48.81825385683782
>50	0.10769000193037509	2.264084825560806
>100	0.03292433180036946	1.6944016923849607
>500	0.004115541475046182	0.877510332510578
>1k	0.0034296178958718184	2.037383388861874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	2782	0.6195439159094959	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	2159	0.48080349189381794	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	1894	0.4217887047924461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCAT	1113	0.2478621058257616	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1093	0.24340815962943166	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	876	0.19508284339925175	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	874	0.19463744877961875	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTA	564	0.12560128273650453	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	534	0.11892036344200961	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	533	0.11869766613219312	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	513	0.11424371993586316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21735257438090147	0.0
2	0.0	0.0	0.0	0.8460270799928736	0.0
3	0.0	0.0	0.0	1.0613753785854267	0.0
4	0.0	0.0	0.0	1.4996436843042935	0.0
5	0.0	0.0	0.0	2.4465526456440405	0.0
6	0.0	0.0	0.0	2.761892036344201	0.0
7	0.0	0.0	0.0	3.134464635667201	0.0
8	0.0	0.0	0.0	3.729957242116515	0.0
9	0.0	0.0	0.0	3.945305540709068	0.0
10	0.0	0.0	0.0	4.921833244254409	0.0
11	0.0	0.0	0.0	5.771200783894531	0.0
12	0.0	0.0	0.0	7.368608587208267	0.0
13	0.0	0.0	0.0	7.707108498129343	0.0
14	0.0	0.0	0.0	7.841617673258507	0.0
15	0.0	0.0	0.0	8.167423837520042	0.0
16	0.0	0.0	0.0	8.5974523427757	0.0
17	0.0	0.0	0.0	9.142169962586852	0.0
18	0.0	0.0	0.0	9.731427044361304	0.0
19	0.0	0.0	0.0	10.457420274363086	0.0
20	0.0	0.0	0.0	10.980981649741672	0.0
21	0.0	0.0	0.0	11.64996436843043	0.0
22	0.0	0.0	0.0	12.29467308034919	0.0
23	0.0	0.0	0.0	12.902191341528594	0.0
24	0.0	0.0	0.0	13.437332977017638	0.0
25	0.0	0.0	0.0	13.91212364154641	0.0
26	0.0	0.0	0.0	14.341484054872618	0.0
27	0.0	0.0	0.0	14.75391947265277	0.0
28	0.0	0.0	0.0	15.192187778371638	0.0
29	0.0	0.0	0.0	15.64025476572243	0.0
30	0.0	0.0	0.0	16.174282914662392	0.0
31	0.0	0.0	0.0	16.66956173169428	0.0
32	0.0	0.0	0.0	17.155709959023696	0.0
33	0.0	0.0	0.0	17.639408515945128	0.0
34	0.0	0.0	0.0	18.128674505611972	0.0
35	0.0	0.0	0.0	18.634865490824872	0.0
36	0.0	0.0	0.0	19.158426866203456	0.0
37	0.0	0.0	0.0	19.686442187778372	0.0
38	0.0	0.0	0.0	20.213121325494388	0.0
39	0.0	0.0	0.0	20.744477106716552	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGG	20	7.0286903E-4	45.0	32
CGAACCC	25	3.8869963E-5	45.0	34
CGAAATA	20	7.0286903E-4	45.0	17
GCGAACC	25	3.8869963E-5	45.0	33
ATTCGCG	20	7.0286903E-4	45.0	1
TACGGGA	90	0.0	45.0	4
TACGCGG	20	7.0286903E-4	45.0	2
ACTACGG	20	7.0286903E-4	45.0	25
TGTTACG	35	1.2097189E-7	45.0	1
TACTACG	20	7.0286903E-4	45.0	24
CGACTCC	20	7.0286903E-4	45.0	28
TACCGGT	20	7.0286903E-4	45.0	40
TTTAGCG	30	2.1625365E-6	44.999996	1
GCCGATC	30	2.1625365E-6	44.999996	9
TTGCGGA	30	2.1625365E-6	44.999996	3
CTACGGC	65	0.0	41.538464	6
CGATGAA	515	0.0	40.631065	19
TCGGGTA	50	1.0786607E-9	40.5	5
CGAATAT	50	1.0786607E-9	40.5	14
TGGGCGA	700	0.0	40.17857	6
>>END_MODULE