##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722394
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.99430919968881	31.0	30.0	31.0	26.0	34.0
2	30.382795261311696	31.0	30.0	33.0	27.0	34.0
3	30.404755576596706	31.0	30.0	34.0	26.0	34.0
4	34.021545029443764	35.0	33.0	37.0	30.0	37.0
5	34.37790319410183	35.0	35.0	37.0	32.0	37.0
6	33.99796925223632	35.0	35.0	37.0	30.0	37.0
7	34.40504489239944	35.0	35.0	37.0	32.0	37.0
8	34.58585481053276	35.0	35.0	37.0	32.0	37.0
9	35.84737553191195	37.0	35.0	39.0	30.0	39.0
10	35.215430914431735	37.0	34.0	39.0	30.0	39.0
11	35.34858816656838	37.0	35.0	39.0	30.0	39.0
12	35.28610149032246	37.0	34.0	39.0	30.0	39.0
13	35.261066675526095	37.0	34.0	39.0	30.0	39.0
14	35.80189342657885	38.0	34.0	40.0	27.0	41.0
15	36.088729972840305	38.0	34.0	40.0	29.0	41.0
16	36.22930838295999	38.0	35.0	40.0	30.0	41.0
17	36.0338928617901	38.0	34.0	40.0	30.0	41.0
18	36.18242953291417	38.0	34.0	40.0	30.0	41.0
19	36.161252723582976	38.0	34.0	40.0	30.0	41.0
20	36.21387497681321	38.0	34.0	40.0	30.0	41.0
21	35.8643330370961	38.0	34.0	40.0	29.0	41.0
22	36.09022500186879	38.0	34.0	40.0	30.0	41.0
23	36.2410235965415	38.0	34.0	40.0	30.0	41.0
24	36.14094801451839	38.0	34.0	40.0	30.0	41.0
25	35.64231015207768	38.0	34.0	40.0	29.0	41.0
26	35.768551510671465	38.0	34.0	40.0	29.0	41.0
27	35.86290583808836	38.0	34.0	40.0	30.0	41.0
28	35.7462977267253	38.0	34.0	40.0	29.0	41.0
29	35.860074972937205	38.0	34.0	40.0	30.0	41.0
30	35.36389421839052	38.0	34.0	40.0	27.0	41.0
31	35.47438655359817	38.0	34.0	40.0	29.0	41.0
32	35.544133810635195	38.0	34.0	40.0	29.0	41.0
33	35.52736733693801	38.0	34.0	40.0	29.0	41.0
34	35.48443231809788	38.0	34.0	40.0	28.0	41.0
35	35.49516745709405	38.0	34.0	40.0	28.0	41.0
36	35.253785330442945	38.0	34.0	40.0	27.0	41.0
37	35.3375830917754	38.0	34.0	40.0	28.0	41.0
38	35.22308463248587	37.0	34.0	40.0	27.0	41.0
39	35.15289994102941	37.0	34.0	40.0	27.0	41.0
40	35.07323842667575	37.0	33.0	40.0	27.0	41.0
41	34.93724200367112	37.0	33.0	40.0	27.0	41.0
42	35.13472703261655	37.0	33.0	40.0	27.0	41.0
43	35.10749535572001	37.0	33.0	40.0	27.0	41.0
44	35.06055144422573	37.0	33.0	40.0	27.0	41.0
45	35.15874993424641	37.0	34.0	40.0	28.0	41.0
46	35.07239954927643	37.0	33.0	40.0	28.0	41.0
47	35.01904362439333	37.0	33.0	40.0	27.0	41.0
48	34.9603678878839	37.0	33.0	40.0	27.0	41.0
49	34.95106825361229	36.0	33.0	40.0	27.0	41.0
50	34.76975999246948	36.0	33.0	40.0	27.0	41.0
51	33.65437005290742	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	9.0
11	8.0
12	12.0
13	16.0
14	26.0
15	57.0
16	109.0
17	204.0
18	325.0
19	642.0
20	1097.0
21	1592.0
22	2440.0
23	3519.0
24	5034.0
25	6881.0
26	8972.0
27	11612.0
28	14705.0
29	18728.0
30	24362.0
31	30429.0
32	38838.0
33	51461.0
34	68193.0
35	69323.0
36	84313.0
37	106944.0
38	120349.0
39	52191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.265143398200983	4.694944863883144	54.79765889528429	17.242252842631583
2	19.861322214747076	3.580732951824074	54.61880912632164	21.9391357071072
3	22.215162362921063	3.5117955021774825	52.28711755634736	21.985924578554087
4	20.98993070263596	5.261948465795674	45.664415817407125	28.083705014161247
5	18.262056440114396	5.694261026531229	50.060216446980455	25.98346608637392
6	22.447722434018004	5.759599332220367	52.33266057027052	19.460017663491115
7	83.81035833630955	2.650769524663826	8.808627978637697	4.73024416038893
8	85.14093417165702	2.7970885693956484	6.77345049931201	5.288526759635324
9	79.31530439067878	5.427370659224744	9.753680124696496	5.50364482539999
10	41.99024355130303	25.653729128425763	16.89715030855738	15.45887701171383
11	31.642012530558116	24.208257543667305	23.987325476125218	20.162404449649358
12	28.290102077259778	21.10828716739065	29.938094723931812	20.663516031417757
13	25.394590763489177	22.771645390188734	30.800920273424197	21.032843572897892
14	20.593748010088678	23.90800588044751	33.53225525128946	21.965990858174347
15	19.163088287001276	23.219461955664084	35.36408109701908	22.253368660315562
16	23.82619456972234	22.868406991198707	30.65349379978239	22.65190463929656
17	24.399012173412295	23.868415296915536	27.752168484234364	23.98040404543781
18	25.174073981788332	22.10483475776377	30.366808140709917	22.354283119737982
19	25.010036074496746	24.69607997851588	27.44956353458085	22.844320412406528
20	26.386708638222355	26.758666323363705	27.3166720653826	19.537952973031338
21	25.144588687059972	25.87770662547031	28.987782290550584	19.989922396919134
22	23.762517407398178	22.76278595890885	30.268800682176213	23.205895951516762
23	23.471402032685766	23.500195184345387	29.356002403120733	23.672400379848117
24	24.225976406227073	23.667832235594428	29.182551350094272	22.92364000808423
25	22.79462454007093	25.300736163367915	26.07690540065394	25.82773389590722
26	21.191898050094547	25.356799751935927	27.22517075169506	26.22613144627447
27	20.794884785864777	23.94994975041321	30.262156108716297	24.993009355005714
28	18.57310553520655	24.940129624553915	29.15888005714333	27.327884783096206
29	20.390396376492607	24.932792908025263	27.951228830804244	26.72558188467789
30	22.639307635445476	24.68832797614598	28.765742794098507	23.906621594310028
31	24.6639645401263	25.153032832498607	26.31583318798329	23.8671694393918
32	24.404964603803464	24.937361052278952	25.60763240004762	25.050041943869967
33	23.891532875411478	24.388214741539933	27.081204993396955	24.63904738965163
34	21.80043023613153	23.744383258997168	28.827481955830198	25.627704549041102
35	21.4839824251032	25.422138057625066	27.269744765321974	25.824134751949767
36	23.18997666093572	26.20052215273106	26.975168675265852	23.63433251106737
37	21.665600766340805	26.322339332829454	27.423400526582448	24.588659374247293
38	21.819118098987534	26.795765191848215	25.98318922914642	25.40192748001783
39	22.06385988809431	25.43376606117991	26.86373364119857	25.638640409527213
40	24.722104557900536	23.96143932535431	27.170214592037034	24.146241524708124
41	21.937751420969718	23.616475219893854	27.00811468533792	27.43765867379851
42	21.207402054834343	22.574661472825078	29.608634623211156	26.609301849129423
43	20.695908327034832	22.89553899949335	29.909163143658446	26.49938952981337
44	21.188714191978338	23.029399468987837	27.604049867523816	28.17783647151001
45	21.568147022262092	22.971674737054848	25.98775737340011	29.47242086728295
46	21.73841421717235	23.526496620957538	28.23306948839553	26.502019673474585
47	19.652018150759833	24.028715631635922	30.61777921743536	25.701487000168882
48	20.073394851009283	24.204519971096104	29.77461053109522	25.94747464679939
49	22.335179971040734	22.403563706232333	29.84368640935556	25.417569913371374
50	21.617842894597686	23.08172548498465	28.002585846504818	27.297845773912847
51	20.857454519278953	22.747005096941557	26.909415083735468	29.48612530004402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	414.0
2	676.0
3	1219.0
4	1762.0
5	1323.0
6	884.0
7	997.0
8	1110.0
9	1173.0
10	1236.0
11	1275.5
12	1315.0
13	1322.5
14	1330.0
15	1392.0
16	1454.0
17	1545.0
18	1636.0
19	1702.5
20	1769.0
21	2099.5
22	2430.0
23	2591.5
24	2753.0
25	3440.5
26	5079.5
27	6031.0
28	6963.5
29	7896.0
30	9275.5
31	10655.0
32	12349.0
33	14043.0
34	16695.0
35	19347.0
36	19700.5
37	20054.0
38	22524.0
39	24994.0
40	26667.0
41	28340.0
42	30769.5
43	33199.0
44	35888.5
45	38578.0
46	41577.5
47	44577.0
48	50911.5
49	57246.0
50	64708.5
51	72171.0
52	71754.5
53	71338.0
54	63404.0
55	55470.0
56	51341.0
57	47212.0
58	43347.5
59	39483.0
60	35187.5
61	30892.0
62	27998.5
63	25105.0
64	21623.5
65	18142.0
66	15178.0
67	12214.0
68	10500.0
69	8786.0
70	7387.0
71	5988.0
72	4670.5
73	3353.0
74	2781.0
75	1783.5
76	1358.0
77	964.5
78	571.0
79	459.0
80	347.0
81	220.5
82	94.0
83	70.0
84	46.0
85	28.5
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	722394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.1313084917889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1141196508489	27.148229318612355
2	10.267250271664425	7.624728743991503
3	3.651719671714213	4.0677938896786365
4	1.8912978854212645	2.8090546093378
5	1.328736929121986	2.466887040983035
6	1.0183204518451714	2.2686942504576555
7	0.8501623014054464	2.2097347077102394
8	0.7880915703146144	2.3410296973704234
9	0.7074734763541581	2.3642474310238115
>10	6.265165864589592	40.26675439957469
>50	0.07542431262896568	1.9440388539163078
>100	0.038466398547462505	2.624585434108719
>500	0.0022627293263213183	0.6423183794620221
>1k	0.0015084862175475456	1.2219032437727502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	2925	0.4049036952134154	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	2010	0.27824151363383415	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1915	0.2650907953277574	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	1876	0.25969207939157857	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.12984603969578928	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	892	0.12347832346337316	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC	837	0.11586474970722348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30509666470098035	0.0
2	0.0	0.0	0.0	1.3586768439383494	0.0
3	0.0	0.0	0.0	1.7538905361893924	0.0
4	0.0	0.0	0.0	2.415717738519423	0.0
5	0.0	0.0	0.0	3.4589157717256787	0.0
6	0.0	0.0	0.0	4.102608825654698	0.0
7	0.0	0.0	0.0	4.732182160981403	0.0
8	0.0	0.0	0.0	5.672250876945268	0.0
9	0.0	0.0	0.0	6.107470438569534	0.0
10	0.0	0.0	0.0	6.899835823664095	0.0
11	0.0	0.0	0.0	8.462141158426011	0.0
12	0.0	0.0	0.0	9.809189998809513	0.0
13	0.0	0.0	0.0	10.314454438990357	0.0
14	0.0	0.0	0.0	10.497595494979194	0.0
15	0.0	0.0	0.0	10.770715149904346	0.0
16	0.0	0.0	0.0	11.387276195538723	0.0
17	0.0	0.0	0.0	12.29827490261547	0.0
18	0.0	0.0	0.0	13.255647195297858	0.0
19	0.0	0.0	0.0	13.904185250708062	0.0
20	0.0	0.0	0.0	14.643809333964567	0.0
21	0.0	0.0	0.0	15.661813359468656	0.0
22	0.0	0.0	0.0	16.696428818622525	0.0
23	0.0	0.0	0.0	17.64480325141128	0.0
24	0.0	0.0	0.0	18.38941076476272	0.0
25	0.0	0.0	0.0	19.095950409333412	0.0
26	0.0	0.0	0.0	19.7266311735701	0.0
27	0.0	0.0	0.0	20.381536945212723	0.0
28	0.0	0.0	0.0	21.046548005658963	0.0
29	0.0	0.0	0.0	21.784787802777984	0.0
30	0.0	0.0	0.0	22.644844779995402	0.0
31	0.0	0.0	0.0	23.33145070418636	0.0
32	0.0	0.0	0.0	23.995769621563856	0.0
33	0.0	0.0	0.0	24.722519843741782	0.0
34	0.0	0.0	0.0	25.489414363906675	0.0
35	0.0	0.0	0.0	26.286624750482424	0.0
36	0.0	0.0	0.0	26.972676960218386	0.0
37	0.0	0.0	0.0	27.67977031924407	0.0
38	0.0	0.0	0.0	28.492899996400855	0.0
39	0.0	0.0	0.0	29.641303776055725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAT	20	7.031544E-4	45.0	17
GCGATAT	35	1.2110831E-7	45.0	9
TTCGCGA	20	7.031544E-4	45.0	2
CTACGAA	95	0.0	42.63158	11
GGCGATA	70	0.0	41.785713	8
TTTGTCG	285	0.0	41.05263	1
TTACACG	385	0.0	40.909092	34
TTGGGAC	1220	0.0	40.758194	5
GCTACGA	100	0.0	40.499996	10
TCGTTCA	90	0.0	40.0	16
CGCGACC	180	0.0	40.0	10
CGAGATA	465	0.0	39.67742	19
AGACACG	115	0.0	39.130436	24
ACACGCG	390	0.0	38.653847	36
TACGAAT	105	0.0	38.57143	12
ACCGGTA	35	6.245067E-6	38.571426	41
CGTTAGG	35	6.245067E-6	38.571426	2
TTTGGGC	1620	0.0	38.333336	4
CGTTTGG	530	0.0	38.207546	2
TACACGC	415	0.0	37.95181	35
>>END_MODULE