##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934044.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785758
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.007288503585073	31.0	30.0	31.0	26.0	34.0
2	30.09099748268551	31.0	30.0	33.0	25.0	34.0
3	30.176138200311037	31.0	30.0	34.0	25.0	34.0
4	33.99310983788902	35.0	33.0	37.0	28.0	37.0
5	34.474968119955506	35.0	35.0	37.0	32.0	37.0
6	33.9050369197641	35.0	35.0	37.0	30.0	37.0
7	34.50854588817422	35.0	35.0	37.0	32.0	37.0
8	34.48529190921378	35.0	35.0	37.0	31.0	37.0
9	35.96162049893224	37.0	35.0	39.0	31.0	39.0
10	35.386736119772245	37.0	34.0	39.0	30.0	39.0
11	35.50887041557324	37.0	35.0	39.0	30.0	39.0
12	35.594401838734065	37.0	35.0	39.0	30.0	39.0
13	35.590706044354626	37.0	35.0	39.0	30.0	39.0
14	36.22818221386228	38.0	35.0	40.0	29.0	41.0
15	36.451188533874294	38.0	35.0	40.0	30.0	41.0
16	36.67152991124494	38.0	35.0	40.0	31.0	41.0
17	36.34762356857964	38.0	35.0	40.0	30.0	41.0
18	36.48122322648958	38.0	35.0	40.0	30.0	41.0
19	36.43431438178167	38.0	35.0	40.0	30.0	41.0
20	36.33095431417816	38.0	35.0	40.0	30.0	41.0
21	36.101143100038435	38.0	34.0	40.0	30.0	41.0
22	36.32070942962083	38.0	35.0	40.0	30.0	41.0
23	36.328123926196106	38.0	35.0	40.0	30.0	41.0
24	36.23624958320501	38.0	35.0	40.0	30.0	41.0
25	35.82299384797864	38.0	34.0	40.0	29.0	41.0
26	35.931696527429565	38.0	34.0	40.0	30.0	41.0
27	35.92641373043609	38.0	34.0	40.0	30.0	41.0
28	35.81447595824669	38.0	34.0	40.0	29.0	41.0
29	35.95544302444264	38.0	34.0	40.0	30.0	41.0
30	35.5050677180506	38.0	34.0	40.0	27.0	41.0
31	35.63575681062108	38.0	34.0	40.0	29.0	41.0
32	35.54659704387356	38.0	34.0	40.0	28.0	41.0
33	35.46887845876211	38.0	34.0	40.0	27.0	41.0
34	35.36216239605578	38.0	34.0	40.0	27.0	41.0
35	35.37773462058293	38.0	34.0	40.0	27.0	41.0
36	35.12075728150398	38.0	34.0	40.0	26.0	41.0
37	35.18264020219966	38.0	34.0	40.0	26.0	41.0
38	35.196085817770864	38.0	34.0	40.0	26.0	41.0
39	35.044800052942506	38.0	34.0	40.0	26.0	41.0
40	35.02696377256102	38.0	34.0	40.0	26.0	41.0
41	34.77894593500798	38.0	33.0	40.0	25.0	41.0
42	35.02066794102001	38.0	34.0	40.0	26.0	41.0
43	35.002686577801306	38.0	34.0	40.0	26.0	41.0
44	34.99288203238147	38.0	34.0	40.0	26.0	41.0
45	35.050444284372546	38.0	34.0	40.0	26.0	41.0
46	34.977290718007325	38.0	34.0	40.0	26.0	41.0
47	34.8876142527343	37.0	34.0	40.0	26.0	41.0
48	34.863508866597606	37.0	34.0	40.0	26.0	41.0
49	34.91154019430919	37.0	34.0	40.0	26.0	41.0
50	34.738287106208276	37.0	33.0	40.0	26.0	41.0
51	33.700294492706405	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	14.0
13	24.0
14	10.0
15	50.0
16	80.0
17	203.0
18	410.0
19	831.0
20	1530.0
21	2632.0
22	4349.0
23	5969.0
24	7554.0
25	9518.0
26	11246.0
27	12899.0
28	14899.0
29	18221.0
30	23463.0
31	30225.0
32	38333.0
33	49784.0
34	65252.0
35	71891.0
36	89187.0
37	113422.0
38	140191.0
39	73561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.334645017931727	3.947780359856343	52.59278301970836	18.12479160250357
2	20.179113671130295	7.917577676587448	52.482698235334546	19.42061041694771
3	18.751320381084252	7.649810755983394	53.74071915271623	19.85814971021612
4	17.936056648484648	4.440807475075023	52.589983175481514	25.033152700958823
5	20.445607935267603	5.103352431664711	51.63498176283283	22.816057870234857
6	20.545384202260745	9.25933938948124	52.91425604320923	17.28102036504878
7	80.87948197791177	1.6586531731143686	13.714400616983854	3.747464231990002
8	82.76174089223399	5.41515326601829	8.046370511022477	3.776735330725236
9	77.69402284163827	5.001921711264791	9.415748869244728	7.888306577852214
10	38.12853321251581	34.57934885804535	15.896624660518889	11.395493268919948
11	26.668007198144977	24.014263933679324	31.13668584984181	18.181043018333888
12	23.709589975539544	21.9614690528127	32.52693577411875	21.80200519752901
13	24.261795616462063	22.72239035428211	34.422812112635185	18.59300191662064
14	19.259492108257252	26.89008575159273	32.962820613980384	20.887601526169636
15	17.305073572270345	25.17594475652809	36.460589647194176	21.058392024007393
16	21.4086270836568	26.03676959063732	30.767360943191164	21.78724238251472
17	21.760898393653008	27.01722413262099	30.310095474688136	20.91178199903787
18	21.29714237716956	24.235451627600355	31.988093026097093	22.479312969132987
19	21.610724930576588	26.413221373501766	29.91315392270903	22.062899773212617
20	23.487893219031815	27.914828738619267	30.828448453595126	17.76882958875379
21	22.792259194306645	28.68822207346282	30.829721110061875	17.68979762216866
22	21.376810671988068	23.856072734862387	33.56402352887275	21.203093064276786
23	21.017157954484713	26.827598319075342	31.32682072597415	20.828423000465794
24	20.03212184922075	27.54626742584867	31.050908803982907	21.37070192094767
25	20.367721359502543	29.874210634826497	28.3583495172814	21.399718488389556
26	19.77033641401042	27.30026293082603	30.38747298786649	22.541927667297056
27	21.333158555178567	26.996988894799674	30.451487608143985	21.21836494187778
28	17.63952769173206	28.02453172605306	31.619913510266517	22.71602707194836
29	19.6739963194775	25.379060728621276	31.930059891213325	23.016883060687896
30	21.680593770601124	27.36325942593012	30.03532894351696	20.920817859951793
31	21.53818351197188	26.607174219034356	28.389529600716763	23.465112668277
32	23.594796362238753	27.076784455264853	27.51992343698696	21.808495745509433
33	22.44940554216438	26.099002491861363	27.407293339679644	24.044298626294612
34	20.205712191285357	26.576884995125727	29.501195024422277	23.71620778916664
35	21.41448130340385	26.821744099328292	28.495287353103627	23.268487244164234
36	21.53283835481153	28.468561567301894	27.669969634416702	22.328630443469873
37	20.738955250853316	27.845596226828107	28.381257333682893	23.034191188635685
38	20.326869086919892	26.9009033315601	27.527559375787458	25.24466820573255
39	19.734320236001416	28.567446974768313	27.54295851903512	24.15527427019515
40	21.771843239267053	26.27501088121279	28.24317410704059	23.70997177247957
41	19.839950722741605	28.178395892883053	27.528577500960854	24.453075883414485
42	21.394373331229207	26.296009712914152	29.738290924177672	22.571326031678964
43	20.171859529269824	27.527304844494104	29.186085283255146	23.114750342980916
44	21.37897418798154	25.743676806344958	29.014658457184016	23.862690548489486
45	20.299379707238106	25.24530453396593	27.656861272809184	26.798454485986777
46	21.25476291682681	27.108091804346884	28.24966465502101	23.387480623805292
47	18.00528916027581	27.060367186843788	31.34680143250212	23.587542220378285
48	19.54634887586254	27.649225334008694	28.980296732581788	23.82412905754698
49	21.42249903914437	25.117020762117598	30.096416453920927	23.364063744817106
50	20.297725253831334	26.324771749062688	28.545430017893548	24.83207297921243
51	19.424046589407933	25.601775610302408	27.96598952858259	27.008188271707066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	304.0
2	474.0
3	3746.5
4	7019.0
5	4820.5
6	2622.0
7	2626.5
8	2631.0
9	2926.0
10	3221.0
11	3417.0
12	3613.0
13	3658.0
14	3703.0
15	3657.5
16	3612.0
17	3553.0
18	3494.0
19	3487.5
20	3481.0
21	3679.5
22	3878.0
23	4177.5
24	4477.0
25	5378.0
26	7881.0
27	9483.0
28	11215.0
29	12947.0
30	15036.0
31	17125.0
32	19723.5
33	22322.0
34	25082.0
35	27842.0
36	29902.0
37	31962.0
38	34025.5
39	36089.0
40	38065.0
41	40041.0
42	42627.0
43	45213.0
44	46722.5
45	48232.0
46	51412.5
47	54593.0
48	67411.5
49	80230.0
50	75990.5
51	71751.0
52	67514.0
53	63277.0
54	55335.0
55	47393.0
56	42238.0
57	37083.0
58	32272.0
59	27461.0
60	23966.5
61	20472.0
62	17738.0
63	15004.0
64	12794.0
65	10584.0
66	8594.5
67	6605.0
68	5429.0
69	4253.0
70	3603.5
71	2954.0
72	2346.0
73	1738.0
74	1420.0
75	841.0
76	580.0
77	552.0
78	524.0
79	340.0
80	156.0
81	100.5
82	45.0
83	40.5
84	36.0
85	24.0
86	12.0
87	11.0
88	10.0
89	5.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.08720293846012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30976849704916	26.816316960630044
2	8.728227916310196	6.29954664218038
3	3.2606850801573906	3.5300701261814664
4	1.8692557919829684	2.698248524367255
5	1.3335025091957613	2.406118783414662
6	1.11096302436071	2.4054928870338212
7	0.9645881554533717	2.436650196251653
8	0.9050791916993697	2.612942117298609
9	0.8356487088046954	2.7140602085907495
>10	6.576249885485748	39.78055013764838
>50	0.0689024582286129	1.7107871850620835
>100	0.029631623515128117	1.924651506110164
>500	0.004284090146765261	1.2274017604063805
>1k	0.0028560600978435077	2.773667733787023
>5k	3.5700751223043846E-4	0.6634952310373529
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	5150	0.6554180803758919	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	4796	0.6103660414529665	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4729	0.6018392431257461	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	3255	0.4142496799268986	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGAT	2258	0.28736583019199297	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2155	0.2742574685844751	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1685	0.2144426146472578	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1556	0.1980253462261918	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTA	1095	0.13935588310904884	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	994	0.1265020527948809	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC	928	0.11810252011433546	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	913	0.1161935354142115	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	912	0.11606626976753656	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	878	0.11173923778058893	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	872	0.11097564390053936	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	838	0.10664861191359172	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23416878988187204	0.0
2	0.0	0.0	0.0	0.8862779634442156	0.0
3	0.0	0.0	0.0	1.1816615293767292	0.0
4	0.0	0.0	0.0	1.716177245411437	0.0
5	0.0	0.0	0.0	2.896184321381392	0.0
6	0.0	0.0	0.0	3.5183860679751273	0.0
7	0.0	0.0	0.0	4.185130790905088	0.0
8	0.0	0.0	0.0	5.063645549902133	0.0
9	0.0	0.0	0.0	5.456260069894293	0.0
10	0.0	0.0	0.0	6.725989426770075	0.0
11	0.0	0.0	0.0	7.818055940887652	0.0
12	0.0	0.0	0.0	9.5600681125741	0.0
13	0.0	0.0	0.0	9.91730278279063	0.0
14	0.0	0.0	0.0	10.090129530975187	0.0
15	0.0	0.0	0.0	10.40956630412926	0.0
16	0.0	0.0	0.0	10.783600039706881	0.0
17	0.0	0.0	0.0	11.335042086749356	0.0
18	0.0	0.0	0.0	11.910919137953416	0.0
19	0.0	0.0	0.0	12.708238414371856	0.0
20	0.0	0.0	0.0	13.204828967697434	0.0
21	0.0	0.0	0.0	13.822194619717521	0.0
22	0.0	0.0	0.0	14.514519737629143	0.0
23	0.0	0.0	0.0	15.171465005765134	0.0
24	0.0	0.0	0.0	15.696435798299222	0.0
25	0.0	0.0	0.0	16.166809628409766	0.0
26	0.0	0.0	0.0	16.598112904991105	0.0
27	0.0	0.0	0.0	17.053723920087354	0.0
28	0.0	0.0	0.0	17.5210433746777	0.0
29	0.0	0.0	0.0	18.03468752465772	0.0
30	0.0	0.0	0.0	18.653834895731254	0.0
31	0.0	0.0	0.0	19.150934511643534	0.0
32	0.0	0.0	0.0	19.64790686190914	0.0
33	0.0	0.0	0.0	20.152515150975237	0.0
34	0.0	0.0	0.0	20.700266494264138	0.0
35	0.0	0.0	0.0	21.30503284726341	0.0
36	0.0	0.0	0.0	21.835476062604517	0.0
37	0.0	0.0	0.0	22.373682482392798	0.0
38	0.0	0.0	0.0	22.97564899116522	0.0
39	0.0	0.0	0.0	23.852891093695515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAATC	20	7.0319226E-4	45.0	43
TCGAATT	20	7.0319226E-4	45.0	18
CCGTTAG	20	7.0319226E-4	45.0	1
GCGATCT	250	0.0	43.2	9
CGTTTTT	2715	0.0	42.928173	1
CGATCGA	155	0.0	42.096775	41
CCGATGA	945	0.0	41.90476	18
CGATGAA	945	0.0	41.42857	19
TCGATTG	60	3.6379788E-12	41.249996	1
TGGGCGA	970	0.0	40.12887	6
TACGGCT	630	0.0	40.0	7
ACGGGAC	90	0.0	40.0	5
TACGAAT	80	0.0	39.375	12
ATACGGA	40	3.4563527E-7	39.375	28
TGCGTTG	160	0.0	39.375	1
GGGCGAT	1555	0.0	39.35691	7
GGCGATT	805	0.0	39.130436	8
TTTCTCG	300	0.0	39.0	1
TTTCGCG	255	0.0	38.82353	1
TTTGGGA	5815	0.0	38.654343	4
>>END_MODULE