SRR2934043.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127081
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	2136	1.6808177461618965	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	2124	1.6713749498351445	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	1303	1.025330301146513	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGCAACT	976	0.7680141012425147	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	593	0.4666315184803393	TruSeq Adapter, Index 18 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTA	480	0.37771185307008914	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	466	0.3666952573555449	RNA PCR Primer, Index 34 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC	394	0.3100384793950315	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	385	0.30295638214996734	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	382	0.3005956830682793	RNA PCR Primer, Index 34 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	338	0.26597209653685444	TruSeq Adapter, Index 18 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	298	0.23449610878101368	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	162	0.12747775041115508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCCACTCGTATGC	161	0.12669085071725908	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGCTCGAATGC	152	0.11960875347219489	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGT	152	0.11960875347219489	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTG	35	1.2005512E-7	45.000004	39
CACCCGT	35	1.2005512E-7	45.000004	38
GTCATAC	40	6.73208E-9	45.0	39
TCGTTTG	40	6.73208E-9	45.0	1
GGGTGGA	20	7.009595E-4	45.0	7
CGGCGAA	25	3.8711798E-5	45.0	31
TCGCAAG	20	7.009595E-4	45.0	29
ACCGGGA	20	7.009595E-4	45.0	4
GTTCTAA	25	3.8711798E-5	45.0	20
AGTTTCG	20	7.009595E-4	45.0	1
AGTCAAC	20	7.009595E-4	45.0	19
AGGAGTA	20	7.009595E-4	45.0	36
CACGGGG	40	6.73208E-9	45.0	4
CCGATAT	20	7.009595E-4	45.0	19
TGGACCG	25	3.8711798E-5	45.0	19
ACCGCTC	40	6.73208E-9	45.0	39
TTTGTCG	40	6.73208E-9	45.0	1
ACCGCGG	25	3.8711798E-5	45.0	39
TTTCGCG	25	3.8711798E-5	45.0	1
GGGTAAT	20	7.009595E-4	45.0	7