##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127081
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.653520195780644	31.0	28.0	31.0	25.0	33.0
2	29.242955280490396	31.0	30.0	33.0	23.0	34.0
3	29.32723223770666	31.0	28.0	33.0	22.0	34.0
4	33.660397699105296	35.0	32.0	37.0	28.0	37.0
5	34.326130578135206	35.0	35.0	37.0	32.0	37.0
6	33.40796027730345	35.0	33.0	37.0	28.0	37.0
7	34.296149699797766	35.0	35.0	36.0	32.0	37.0
8	33.69105531117948	35.0	35.0	37.0	28.0	37.0
9	35.58937213273424	37.0	34.0	39.0	30.0	39.0
10	35.18573980374722	37.0	34.0	39.0	30.0	39.0
11	35.25561649656518	37.0	34.0	39.0	30.0	39.0
12	34.9648964046553	37.0	34.0	39.0	30.0	39.0
13	34.52768706572973	37.0	33.0	39.0	27.0	39.0
14	35.07691944507834	37.0	33.0	40.0	25.0	41.0
15	35.50648011897923	38.0	33.0	40.0	27.0	41.0
16	36.06682352200565	38.0	34.0	40.0	30.0	41.0
17	35.68959167774884	37.0	34.0	40.0	29.0	41.0
18	35.89805714465577	37.0	34.0	40.0	30.0	41.0
19	35.84092822687892	38.0	34.0	40.0	30.0	41.0
20	35.57842635799215	37.0	34.0	40.0	29.0	41.0
21	35.481094734854146	37.0	34.0	40.0	29.0	41.0
22	35.711302240303425	37.0	34.0	40.0	29.0	41.0
23	35.59706801174055	37.0	34.0	40.0	29.0	41.0
24	35.54029319882594	37.0	34.0	40.0	29.0	41.0
25	35.2877534800639	37.0	34.0	40.0	28.0	41.0
26	35.32518629850253	37.0	34.0	40.0	29.0	41.0
27	35.01653276256876	37.0	33.0	40.0	27.0	41.0
28	34.90181065619566	37.0	33.0	40.0	27.0	41.0
29	35.10974103131074	37.0	34.0	40.0	27.0	41.0
30	34.77169679180995	36.0	33.0	40.0	26.0	41.0
31	34.94439766762931	37.0	33.0	40.0	27.0	41.0
32	34.74383267364909	37.0	33.0	40.0	26.0	41.0
33	34.6499161951826	37.0	33.0	40.0	25.0	41.0
34	34.50644077399454	37.0	33.0	40.0	25.0	41.0
35	34.54453458817604	37.0	33.0	40.0	25.0	41.0
36	34.254625002950874	36.0	33.0	40.0	24.0	41.0
37	34.18632997851764	36.0	33.0	40.0	24.0	41.0
38	34.30505740433267	36.0	33.0	40.0	24.0	41.0
39	34.23894996104846	36.0	33.0	40.0	24.0	41.0
40	34.30172094963055	36.0	33.0	40.0	24.0	41.0
41	34.07059277153941	36.0	33.0	39.0	24.0	41.0
42	34.16687781808453	36.0	33.0	40.0	24.0	41.0
43	34.099944130121735	36.0	33.0	40.0	24.0	41.0
44	34.24180640693731	36.0	33.0	40.0	24.0	41.0
45	34.3116280167767	36.0	33.0	40.0	25.0	41.0
46	34.17824064966439	36.0	33.0	40.0	24.0	41.0
47	33.90231427199975	36.0	33.0	39.0	23.0	41.0
48	33.97372541922081	36.0	33.0	39.0	24.0	41.0
49	34.13048370724184	36.0	33.0	39.0	24.0	41.0
50	33.87247503560721	36.0	33.0	39.0	24.0	41.0
51	32.91366923458267	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	1.0
13	3.0
14	7.0
15	7.0
16	13.0
17	26.0
18	68.0
19	187.0
20	471.0
21	782.0
22	1147.0
23	1348.0
24	1602.0
25	1749.0
26	1914.0
27	2233.0
28	2759.0
29	3572.0
30	4621.0
31	6132.0
32	7493.0
33	9780.0
34	14264.0
35	12519.0
36	12779.0
37	15504.0
38	17800.0
39	8294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.20072237391899	4.168207678567213	48.840503301044215	14.790566646469575
2	17.473894602654997	15.516088164241705	47.40991965754125	19.600097575562042
3	17.70524311266043	15.646713513428443	47.15653795610674	19.49150541780439
4	19.19720493228728	4.766251445928187	53.25343678441309	22.783106837371438
5	27.306993177579653	4.944877676442585	44.52121088124897	23.226918264728795
6	19.445078335864526	15.735633178838693	47.34618078233568	17.473107702961105
7	74.7397329262439	2.5204397195489494	18.689654629724348	4.0501727244828105
8	75.25515222574577	12.041926015690779	7.0569164548595	5.646005303703936
9	70.56365625073772	4.667888984191185	8.76212809153217	16.006326673538922
10	48.63905697940684	22.44159237022057	15.095883727701228	13.823466922671368
11	40.21686955563774	21.81285951479765	22.940486776150646	15.029784153413964
12	32.81056963668841	18.19076022379427	25.244529079878188	23.754141059639128
13	27.521816794013265	27.60286746248456	29.0964030814992	15.778912662002975
14	16.80345606345559	37.32580008026377	25.438893304270504	20.431850552010136
15	13.573232820012432	24.241231970160765	41.52469684689293	20.660838362933877
16	16.144034119970726	21.234488239784074	35.67488452246992	26.946593117775276
17	15.218640079949008	25.926771114486037	30.512822530512036	28.341766275052922
18	17.602946152453946	21.956075259086724	32.58158182576467	27.85939676269466
19	21.896270882350628	24.832193640276675	27.358928557376792	25.912606919995905
20	29.30257080129996	22.928683280742206	29.56146080059175	18.20728511736609
21	23.0482920342144	30.856697696744597	26.70029351358582	19.394716755455182
22	18.85332976605472	23.11675230758335	32.40689009371975	25.623027832642176
23	22.157521580724104	28.213501624947867	26.08651175234693	23.542465041981096
24	21.47291884703457	25.2626277728378	29.751890526514586	23.51256285361305
25	16.522532872734715	31.322542315531038	28.28825709586799	23.866667715866257
26	17.920066729094042	27.373092751866917	31.482282953391934	23.224557565647107
27	21.823089210818296	31.06443921593314	26.615308346645055	20.497163226603504
28	17.244906791731257	30.656825174495005	33.4455976896625	18.652670344111236
29	20.9921231340641	22.491953950629913	34.42686160795084	22.089061307355152
30	17.972789008585075	30.360950889590104	30.03753511539884	21.62872498642598
31	26.338319654393654	26.100675946837054	22.28106483266578	25.27993956610351
32	30.518330828369304	28.27015840290838	22.468346959813033	18.743163808909276
33	25.492402483455436	25.6175195347849	23.185212580952307	25.704865400807357
34	24.194017988527	27.164564332984476	23.985489569644557	24.655928108843966
35	22.875174101557274	25.75837457999229	27.201548618597588	24.16490269985285
36	26.488617495927798	26.436682116130655	25.13908452089612	21.935615867045428
37	22.853927809822082	25.870114336525525	30.375901983774128	20.90005586987827
38	22.475429057058097	26.458715307559743	22.84841951196481	28.21743612341735
39	23.11911300666504	30.129602379584675	24.84478403537901	21.906500578371276
40	20.95120434998151	26.279302177351454	25.28544786396078	27.48404560870626
41	17.316514663875797	26.713670808382055	25.298825158757015	30.670989368985136
42	23.307968933200087	25.77804707233969	26.354057648271574	24.55992634618865
43	24.800717652520834	23.132490301461274	23.493677260959544	28.57311478505835
44	21.35567079264406	25.06275525058821	30.059568306827927	23.522005649939803
45	21.02202532243215	26.916690929407228	24.699207592008246	27.362076156152376
46	25.5781745500901	29.5709035969185	24.72045388374344	20.13046796924796
47	18.77463979666512	27.561948678401965	30.307441710405175	23.35596981452774
48	22.21417835868462	25.614371936009316	27.635130349934293	24.536319355371774
49	22.148078784397356	21.56734681030209	32.39902109678079	23.885553308519764
50	20.827661098039833	25.04859105609808	26.772688285424255	27.35105956043783
51	18.99811930973159	26.6664568267483	25.619093334172693	28.716330529347424
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	44.5
2	71.0
3	137.5
4	204.0
5	172.0
6	140.0
7	149.5
8	159.0
9	184.0
10	209.0
11	255.0
12	301.0
13	305.0
14	309.0
15	290.5
16	272.0
17	242.0
18	212.0
19	270.5
20	329.0
21	466.5
22	604.0
23	578.0
24	552.0
25	521.5
26	784.0
27	1077.0
28	1266.0
29	1455.0
30	1548.0
31	1641.0
32	2084.0
33	2527.0
34	2806.5
35	3086.0
36	3454.5
37	3823.0
38	3881.0
39	3939.0
40	5015.5
41	6092.0
42	7538.0
43	8984.0
44	8882.5
45	8781.0
46	9165.0
47	9549.0
48	13869.0
49	18189.0
50	16202.0
51	14215.0
52	12679.0
53	11143.0
54	9363.5
55	7584.0
56	6825.0
57	6066.0
58	5283.5
59	4501.0
60	4089.0
61	3677.0
62	3132.5
63	2588.0
64	2052.0
65	1516.0
66	1336.0
67	1156.0
68	943.0
69	730.0
70	550.0
71	370.0
72	319.5
73	269.0
74	188.5
75	88.5
76	69.0
77	46.5
78	24.0
79	26.5
80	29.0
81	22.5
82	16.0
83	10.5
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.92418221449312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35302600153216	26.6656699270544
2	9.080257762155828	6.342411532801914
3	3.035014194943896	3.179861663033813
4	1.5276463431120724	2.1340719698460036
5	0.8742282907485016	1.526585406158277
6	0.7593168401604254	1.5911111810577507
7	0.6421522238745437	1.569864889322558
8	0.5723040872425758	1.5989801779967105
9	0.5137217790996349	1.6147181718746313
>10	6.480104546888378	42.10936331945767
>50	0.10364562209904916	2.4889637317931084
>100	0.04731647965391375	3.5662294127367584
>500	0.004506331395610833	1.234645619722854
>1k	0.00675949709341625	4.377522997143554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	2136	1.6808177461618965	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	2124	1.6713749498351445	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	1303	1.025330301146513	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGCAACT	976	0.7680141012425147	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	593	0.4666315184803393	TruSeq Adapter, Index 18 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTA	480	0.37771185307008914	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	466	0.3666952573555449	RNA PCR Primer, Index 34 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTC	394	0.3100384793950315	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCT	385	0.30295638214996734	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	382	0.3005956830682793	RNA PCR Primer, Index 34 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	338	0.26597209653685444	TruSeq Adapter, Index 18 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	298	0.23449610878101368	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	162	0.12747775041115508	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCCACTCGTATGC	161	0.12669085071725908	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGCTCGAATGC	152	0.11960875347219489	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGT	152	0.11960875347219489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3454489656203524	0.0
2	0.0	0.0	0.0	1.6218002691196953	0.0
3	0.0	0.0	0.0	1.973544432291216	0.0
4	0.0	0.0	0.0	2.7801166185346355	0.0
5	0.0	0.0	0.0	4.777268041642732	0.0
6	0.0	0.0	0.0	5.3288847270638415	0.0
7	0.0	0.0	0.0	6.113423721878172	0.0
8	0.0	0.0	0.0	7.150557518433125	0.0
9	0.0	0.0	0.0	7.465317395991533	0.0
10	0.0	0.0	0.0	9.975527419519834	0.0
11	0.0	0.0	0.0	11.124400972608022	0.0
12	0.0	0.0	0.0	14.217703669313273	0.0
13	0.0	0.0	0.0	14.769320354734383	0.0
14	0.0	0.0	0.0	15.054964943618637	0.0
15	0.0	0.0	0.0	15.734059379450901	0.0
16	0.0	0.0	0.0	16.37538262997616	0.0
17	0.0	0.0	0.0	17.162282323872176	0.0
18	0.0	0.0	0.0	18.144333141854407	0.0
19	0.0	0.0	0.0	19.657541253216454	0.0
20	0.0	0.0	0.0	20.282339610169892	0.0
21	0.0	0.0	0.0	20.966155444165533	0.0
22	0.0	0.0	0.0	21.616134591323643	0.0
23	0.0	0.0	0.0	22.30073732501318	0.0
24	0.0	0.0	0.0	22.77759853951417	0.0
25	0.0	0.0	0.0	23.2064588726875	0.0
26	0.0	0.0	0.0	23.584957625451484	0.0
27	0.0	0.0	0.0	23.968177776378845	0.0
28	0.0	0.0	0.0	24.40805470526672	0.0
29	0.0	0.0	0.0	24.87232552466537	0.0
30	0.0	0.0	0.0	25.452270599066736	0.0
31	0.0	0.0	0.0	26.035363272243686	0.0
32	0.0	0.0	0.0	26.50514238949961	0.0
33	0.0	0.0	0.0	26.9615442119593	0.0
34	0.0	0.0	0.0	27.450995821562625	0.0
35	0.0	0.0	0.0	27.966415121064518	0.0
36	0.0	0.0	0.0	28.43068594046317	0.0
37	0.0	0.0	0.0	28.939023142719996	0.0
38	0.0	0.0	0.0	29.402507062424753	0.0
39	0.0	0.0	0.0	29.891171772334182	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTG	35	1.2005512E-7	45.000004	39
CACCCGT	35	1.2005512E-7	45.000004	38
GTCATAC	40	6.73208E-9	45.0	39
TCGTTTG	40	6.73208E-9	45.0	1
GGGTGGA	20	7.009595E-4	45.0	7
CGGCGAA	25	3.8711798E-5	45.0	31
TCGCAAG	20	7.009595E-4	45.0	29
ACCGGGA	20	7.009595E-4	45.0	4
GTTCTAA	25	3.8711798E-5	45.0	20
AGTTTCG	20	7.009595E-4	45.0	1
AGTCAAC	20	7.009595E-4	45.0	19
AGGAGTA	20	7.009595E-4	45.0	36
CACGGGG	40	6.73208E-9	45.0	4
CCGATAT	20	7.009595E-4	45.0	19
TGGACCG	25	3.8711798E-5	45.0	19
ACCGCTC	40	6.73208E-9	45.0	39
TTTGTCG	40	6.73208E-9	45.0	1
ACCGCGG	25	3.8711798E-5	45.0	39
TTTCGCG	25	3.8711798E-5	45.0	1
GGGTAAT	20	7.009595E-4	45.0	7
>>END_MODULE