##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427990
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.791861959391575	31.0	28.0	31.0	25.0	34.0
2	29.39653262926704	31.0	30.0	33.0	23.0	34.0
3	29.526168835720462	31.0	30.0	33.0	23.0	34.0
4	33.765936120002806	35.0	33.0	37.0	28.0	37.0
5	34.380174770438565	35.0	35.0	37.0	32.0	37.0
6	33.42371550737167	35.0	33.0	37.0	28.0	37.0
7	34.27460921984159	35.0	35.0	37.0	32.0	37.0
8	33.74237482184163	35.0	35.0	37.0	28.0	37.0
9	35.59695086333793	37.0	34.0	39.0	30.0	39.0
10	35.184581415453636	37.0	34.0	39.0	30.0	39.0
11	35.35533073202645	37.0	35.0	39.0	30.0	39.0
12	35.36995957849482	37.0	35.0	39.0	30.0	39.0
13	35.21732984415524	37.0	34.0	39.0	29.0	39.0
14	35.986389868922174	38.0	34.0	40.0	29.0	41.0
15	36.19024743568775	38.0	34.0	40.0	30.0	41.0
16	36.50775485408538	38.0	35.0	40.0	31.0	41.0
17	36.13725320685063	38.0	34.0	40.0	30.0	41.0
18	36.31837426108087	38.0	35.0	40.0	30.0	41.0
19	36.23210121731816	38.0	34.0	40.0	30.0	41.0
20	35.98324727213252	38.0	34.0	40.0	29.0	41.0
21	36.00961938363046	38.0	34.0	40.0	30.0	41.0
22	36.14166452487208	38.0	34.0	40.0	30.0	41.0
23	35.926837075632605	38.0	34.0	40.0	29.0	41.0
24	35.9202504731419	38.0	34.0	40.0	29.0	41.0
25	35.6945279095306	38.0	34.0	40.0	29.0	41.0
26	35.678868665155726	38.0	34.0	40.0	29.0	41.0
27	35.444437954157806	38.0	34.0	40.0	27.0	41.0
28	35.33277646673988	38.0	34.0	40.0	27.0	41.0
29	35.565634711091384	38.0	34.0	40.0	27.0	41.0
30	35.243601486016026	37.0	34.0	40.0	27.0	41.0
31	35.360697679852336	38.0	34.0	40.0	27.0	41.0
32	35.116255052688146	38.0	34.0	40.0	26.0	41.0
33	35.02461973410594	38.0	34.0	40.0	26.0	41.0
34	35.04052664781887	38.0	34.0	40.0	26.0	41.0
35	34.95549896025608	38.0	34.0	40.0	25.0	41.0
36	34.723369704899646	37.0	33.0	40.0	25.0	41.0
37	34.545629570784364	37.0	33.0	40.0	24.0	41.0
38	34.64394495198486	37.0	33.0	40.0	24.0	41.0
39	34.67429612841421	37.0	33.0	40.0	25.0	41.0
40	34.85370919881306	37.0	33.0	40.0	26.0	41.0
41	34.4296852730204	37.0	33.0	40.0	24.0	41.0
42	34.593044230005376	37.0	33.0	40.0	24.0	41.0
43	34.339617748078226	37.0	33.0	40.0	24.0	41.0
44	34.56497815369518	37.0	33.0	40.0	24.0	41.0
45	34.52059627561392	37.0	33.0	40.0	24.0	41.0
46	34.508385709946495	37.0	33.0	40.0	24.0	41.0
47	34.34210378747167	37.0	33.0	40.0	24.0	41.0
48	34.34935395686815	37.0	33.0	40.0	24.0	41.0
49	34.433234421364986	37.0	33.0	40.0	24.0	41.0
50	34.04149162363607	36.0	33.0	40.0	23.0	41.0
51	33.204694034907355	36.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	11.0
11	12.0
12	9.0
13	14.0
14	16.0
15	19.0
16	53.0
17	107.0
18	171.0
19	455.0
20	1094.0
21	2156.0
22	3740.0
23	4921.0
24	6065.0
25	6414.0
26	6380.0
27	6831.0
28	8233.0
29	10621.0
30	13650.0
31	17621.0
32	22084.0
33	28198.0
34	38372.0
35	40679.0
36	47530.0
37	58395.0
38	68745.0
39	35389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.08159069137129	3.8685483305684714	48.3665506203416	15.68331035771864
2	17.375639617748078	14.613425547325873	49.062828570761	18.94810626416505
3	19.532465711815696	14.457814434916704	45.78611649804902	20.223603355218582
4	18.20579920091591	4.510853057314423	52.28510011916166	24.998247622608005
5	26.22047244094488	5.706208088974042	44.421832285801074	23.651487184280008
6	20.989509100679925	15.606205752470853	47.01184607116989	16.392439075679338
7	77.1742330428281	2.4675810182480897	16.570480618706043	3.7877053202177624
8	77.82892123647748	11.617327507652048	5.812752634407346	4.740998621463119
9	72.72810112385804	4.983994953153111	7.4303137923783265	14.857590130610529
10	45.40503282786981	22.91992803570177	15.616720016822825	16.0583191196056
11	35.23166429122176	22.68510946517442	22.461739760274774	19.62148648332905
12	26.142900535059233	19.433164326269306	27.458118180331315	26.96581695834015
13	26.757400873852195	22.21266852029253	30.79955139138765	20.23037921446763
14	19.518213042360802	30.56800392532536	27.166989882941188	22.74679314937265
15	15.819294843337461	22.966658099488306	36.07537559288768	25.13867146428655
16	18.245052454496598	22.603565503866914	29.982943526717914	29.16843851491857
17	19.444379541578076	26.48286174910629	29.46821187410921	24.60454683520643
18	19.515642888852543	21.950980163087923	29.92079254188182	28.612584406177717
19	22.33720414028365	24.253370405850603	26.880534591929717	26.528890861936027
20	28.05976775158298	23.39844388887591	29.555129792752165	18.986658566788943
21	24.605948737120027	29.90864272529732	25.391714759690647	20.093693777892007
22	22.148414682586044	21.854015280730856	30.739035958784083	25.258534077899014
23	25.780742540713568	25.818126591742796	26.036589639944857	22.364541227598774
24	21.84794037243861	26.759270076403652	27.573541437884064	23.819248113273677
25	19.6761606579593	31.16568144115517	26.449683403817847	22.708474497067687
26	19.446015093810605	25.0353980233183	31.476202715016704	24.04238416785439
27	23.947989439005585	26.963947755788688	26.33846585200589	22.74959695319984
28	16.94081637421435	30.213790041823408	30.565200121498165	22.280193462464077
29	19.548120283184186	23.382088366550622	31.373396574686325	25.696394775578867
30	18.71025023949158	31.650739503259423	27.1985326759971	22.4404775812519
31	21.911259608869365	27.04782822028552	23.273207317927987	27.767704852917124
32	27.057641533680695	27.873782097712564	23.809434799878503	21.25914156872824
33	24.759924297296664	25.07605317881259	24.375102222014533	25.78892030187621
34	21.947475408303934	26.448982452861046	24.045421622000514	27.558120516834506
35	24.786794083973927	25.424659454660155	25.424659454660155	24.363887006705763
36	20.914974648940397	29.178952779270546	25.735881679478496	24.17019089231057
37	19.62242108460478	30.874085843127176	26.689875931680646	22.813617140587397
38	20.755157830790438	27.811397462557537	23.922054253604056	27.51139045304797
39	20.088319820556556	32.169209560971055	25.13166195471857	22.610808663753826
40	21.53040958900909	24.262950068926845	25.705039837379378	28.50160050468469
41	19.081053295637748	27.015584476272807	25.471389518446692	28.43197270964275
42	24.817168625435173	24.07275870931564	26.547816537769574	24.562256127479614
43	20.23575317180308	27.22727166522582	26.400850487160916	26.136124675810184
44	20.910768943199606	26.444075796163464	28.78525199186897	23.85990326876796
45	20.597443865510876	26.5987523072969	25.540550012850765	27.26325381434146
46	23.466903432323186	29.2915722329961	25.21437416762074	22.02715016705998
47	18.426832402626232	28.398327063716444	30.07500175237739	23.099838781279935
48	20.501647234748475	27.792238136405057	26.772821794901752	24.933292833944716
49	22.282997266291268	23.486062758475665	30.82525292647024	23.40568704876282
50	22.187200635528868	26.08916096170471	27.19058856515339	24.533049837613028
51	20.109815649898362	24.825112736278886	26.831935325591722	28.23313628823103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	184.5
2	325.0
3	530.0
4	735.0
5	570.0
6	405.0
7	407.0
8	409.0
9	471.0
10	533.0
11	518.5
12	504.0
13	477.5
14	451.0
15	514.5
16	578.0
17	596.5
18	615.0
19	812.5
20	1010.0
21	943.0
22	876.0
23	1194.0
24	1512.0
25	1989.5
26	2976.5
27	3486.0
28	3916.0
29	4346.0
30	5551.0
31	6756.0
32	7922.0
33	9088.0
34	9699.5
35	10311.0
36	11436.5
37	12562.0
38	14657.0
39	16752.0
40	17919.5
41	19087.0
42	21967.5
43	24848.0
44	37636.5
45	50425.0
46	45325.5
47	40226.0
48	39058.5
49	37891.0
50	35349.5
51	32808.0
52	31138.0
53	29468.0
54	27408.0
55	25348.0
56	23370.5
57	21393.0
58	20467.0
59	19541.0
60	17464.5
61	15388.0
62	13656.5
63	11925.0
64	10629.0
65	9333.0
66	7647.0
67	5961.0
68	5071.5
69	4182.0
70	3378.5
71	2575.0
72	2018.0
73	1461.0
74	1232.0
75	876.0
76	749.0
77	532.0
78	315.0
79	261.0
80	207.0
81	136.5
82	66.0
83	41.0
84	16.0
85	12.0
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.578300069562545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52132200096199	22.042140237249466
2	9.105190249856904	5.386320988014454
3	3.139415227360242	2.7857569691344546
4	1.5158062111004318	1.7933988383694095
5	1.0529737696497394	1.5572587062039214
6	0.7610516394295578	1.3506368255687988
7	0.6090207753436591	1.260965946919867
8	0.5218723375176021	1.2348877277679728
9	0.5210650120343358	1.3870995553531622
>10	8.044497257042245	49.54575434540195
>50	0.15024491424699105	2.908685456559729
>100	0.04795050454691203	2.746290746388535
>500	0.003995875378909336	0.8743785049828879
>1k	0.003995875378909336	2.3137109507360116
>5k	0.0015983501515637343	2.8127142013493875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC	6657	1.5554101731348864	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	5242	1.2247949718451365	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	3672	0.8579639711208206	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAACAGTT	2363	0.5521157036379355	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1351	0.3156615808780579	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA	1335	0.3119231757751349	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	1067	0.24930489030117525	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC	986	0.23037921446762774	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	879	0.2053786303418304	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	678	0.15841491623636067	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	598	0.13972289072174585	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	558	0.13037687796443842	No Hit
TCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	485	0.11332040468235238	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGT	432	0.10093693777892007	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	430	0.10046963714105471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16005046846888946	0.0
2	0.0	0.0	0.0	0.8614687259048108	0.0
3	0.0	0.0	0.0	1.0577349938082665	0.0
4	0.0	0.0	0.0	1.6572817121895371	0.0
5	0.0	0.0	0.0	3.139792985817426	0.0
6	0.0	0.0	0.0	3.4241454239585036	0.0
7	0.0	0.0	0.0	3.9099044370195566	0.0
8	0.0	0.0	0.0	4.596836374681652	0.0
9	0.0	0.0	0.0	4.797308348325895	0.0
10	0.0	0.0	0.0	6.595247552512909	0.0
11	0.0	0.0	0.0	7.362321549568915	0.0
12	0.0	0.0	0.0	10.048365616019066	0.0
13	0.0	0.0	0.0	10.404682352391411	0.0
14	0.0	0.0	0.0	10.557956961611252	0.0
15	0.0	0.0	0.0	11.092548891329237	0.0
16	0.0	0.0	0.0	11.541157503679992	0.0
17	0.0	0.0	0.0	12.070609126381457	0.0
18	4.673006378653707E-4	0.0	0.0	12.629033388630576	0.0
19	4.673006378653707E-4	0.0	0.0	13.706161358910254	0.0
20	4.673006378653707E-4	0.0	0.0	14.190284819738778	0.0
21	4.673006378653707E-4	0.0	0.0	14.767401107502511	0.0
22	4.673006378653707E-4	0.0	0.0	15.37722843991682	0.0
23	4.673006378653707E-4	0.0	0.0	15.950139021939766	0.0
24	4.673006378653707E-4	0.0	0.0	16.455991962429028	0.0
25	4.673006378653707E-4	0.0	0.0	16.87819808874039	0.0
26	4.673006378653707E-4	0.0	0.0	17.244094488188978	0.0
27	4.673006378653707E-4	0.0	0.0	17.605785181896774	0.0
28	4.673006378653707E-4	0.0	0.0	18.007897380779927	0.0
29	4.673006378653707E-4	0.0	0.0	18.432673660599548	0.0
30	4.673006378653707E-4	0.0	0.0	18.975677001799106	0.0
31	4.673006378653707E-4	0.0	0.0	19.43176242435571	0.0
32	4.673006378653707E-4	0.0	0.0	19.86448281501904	0.0
33	4.673006378653707E-4	0.0	0.0	20.32103553821351	0.0
34	4.673006378653707E-4	0.0	0.0	20.755858781747236	0.0
35	4.673006378653707E-4	0.0	0.0	21.206570246968386	0.0
36	4.673006378653707E-4	0.0	0.0	21.665693123671115	0.0
37	4.673006378653707E-4	0.0	0.0	22.11009603028108	0.0
38	4.673006378653707E-4	0.0	0.0	22.5834715764387	0.0
39	4.673006378653707E-4	0.0	0.0	23.04983761302834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	20	7.028318E-4	45.000004	32
CGGGTCG	20	7.028318E-4	45.000004	6
CGTATGA	30	2.1622964E-6	45.000004	45
TTTACCG	20	7.028318E-4	45.000004	1
TCGGGTA	30	2.1622964E-6	45.000004	5
AGGGTAC	30	2.1622964E-6	45.000004	6
AGCGATA	20	7.028318E-4	45.000004	34
CGAATGT	20	7.028318E-4	45.000004	45
TCATCGA	20	7.028318E-4	45.000004	16
TCGACAA	20	7.028318E-4	45.000004	19
CGGGTAT	50	2.1827873E-11	45.0	6
CGACGGA	25	3.8866878E-5	45.0	41
CCGATCG	330	0.0	45.0	40
ACCGCGT	45	3.8380676E-10	45.0	39
GTTAGCG	25	3.8866878E-5	45.0	1
TACGCCG	25	3.8866878E-5	45.0	29
CCGCGTA	45	3.8380676E-10	45.0	40
GCGTAAG	35	1.209537E-7	45.0	42
GATAGTA	25	3.8866878E-5	45.0	9
ATCGAAT	285	0.0	45.0	43
>>END_MODULE