##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934041.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550719 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.90807653267819 31.0 30.0 31.0 25.0 34.0 2 29.85777864936565 31.0 30.0 33.0 25.0 34.0 3 29.880774042660594 31.0 30.0 34.0 25.0 34.0 4 33.85069699792453 35.0 33.0 37.0 28.0 37.0 5 34.40122821257302 35.0 35.0 37.0 32.0 37.0 6 33.700233694497555 35.0 33.0 37.0 28.0 37.0 7 34.32680187173495 35.0 35.0 37.0 32.0 37.0 8 34.141373368269484 35.0 35.0 37.0 30.0 37.0 9 35.759810357006025 37.0 35.0 39.0 30.0 39.0 10 35.266379042669676 37.0 34.0 39.0 30.0 39.0 11 35.368207742968735 37.0 35.0 39.0 30.0 39.0 12 35.39062934091615 37.0 35.0 39.0 30.0 39.0 13 35.32527477715495 37.0 34.0 39.0 30.0 39.0 14 36.07975029007534 38.0 35.0 40.0 29.0 41.0 15 36.298142973095175 38.0 35.0 40.0 30.0 41.0 16 36.54039174243126 38.0 35.0 40.0 31.0 41.0 17 36.20183069768793 38.0 34.0 40.0 30.0 41.0 18 36.344255418825206 38.0 35.0 40.0 30.0 41.0 19 36.30781396683245 38.0 35.0 40.0 30.0 41.0 20 36.21211906616623 38.0 34.0 40.0 30.0 41.0 21 36.04056152048504 38.0 34.0 40.0 30.0 41.0 22 36.21083892148264 38.0 34.0 40.0 30.0 41.0 23 36.15275848481712 38.0 34.0 40.0 30.0 41.0 24 36.13214906331541 38.0 34.0 40.0 30.0 41.0 25 35.765895129821196 38.0 34.0 40.0 29.0 41.0 26 35.826069193182 38.0 34.0 40.0 29.0 41.0 27 35.746538615882145 38.0 34.0 40.0 29.0 41.0 28 35.62351217226934 38.0 34.0 40.0 28.0 41.0 29 35.771199105169785 38.0 34.0 40.0 29.0 41.0 30 35.39863160704461 38.0 34.0 40.0 27.0 41.0 31 35.604504293478165 38.0 34.0 40.0 29.0 41.0 32 35.45635251371389 38.0 34.0 40.0 27.0 41.0 33 35.43092212180804 38.0 34.0 40.0 27.0 41.0 34 35.343612622771325 38.0 34.0 40.0 27.0 41.0 35 35.31652984552921 38.0 34.0 40.0 27.0 41.0 36 35.154835769239845 38.0 34.0 40.0 26.0 41.0 37 35.1974418896025 38.0 34.0 40.0 27.0 41.0 38 35.08950662679152 38.0 34.0 40.0 26.0 41.0 39 35.01145593306205 37.0 34.0 40.0 27.0 41.0 40 34.96529446051434 37.0 33.0 40.0 26.0 41.0 41 34.83586003025136 37.0 33.0 40.0 26.0 41.0 42 34.90680365122685 37.0 33.0 40.0 26.0 41.0 43 34.87922515838386 37.0 33.0 40.0 26.0 41.0 44 34.95462477234307 37.0 34.0 40.0 27.0 41.0 45 34.990906433226385 37.0 34.0 40.0 27.0 41.0 46 34.86195319209978 37.0 34.0 40.0 26.0 41.0 47 34.697613483464345 37.0 33.0 40.0 26.0 41.0 48 34.69371675936367 37.0 33.0 40.0 26.0 41.0 49 34.71706078780649 37.0 33.0 40.0 26.0 41.0 50 34.520169088046714 36.0 33.0 40.0 26.0 41.0 51 33.45850243045909 35.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 10.0 11 8.0 12 10.0 13 21.0 14 17.0 15 32.0 16 61.0 17 128.0 18 311.0 19 740.0 20 1499.0 21 2596.0 22 3509.0 23 4421.0 24 5149.0 25 5999.0 26 7077.0 27 8336.0 28 10282.0 29 13180.0 30 17018.0 31 22048.0 32 28191.0 33 36828.0 34 50442.0 35 53026.0 36 62887.0 37 79165.0 38 92285.0 39 45438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.650001180275243 4.361934126115133 49.98919594203215 16.998868751577483 2 18.90038295392024 9.993299668251868 51.367757422569404 19.738559955258488 3 21.10531868339389 10.379522043001966 47.93370121604666 20.581458057557484 4 19.07942162881615 4.930645211078608 49.673790081693205 26.31614307841204 5 23.377439311155054 5.686021364797655 46.327618985362776 24.60892033868452 6 21.620826592145903 11.464104198329819 48.515122957442905 18.39994625208137 7 81.03570060230354 2.43481703010065 12.882795037033407 3.64668733056241 8 82.11610639908919 7.777105928794903 5.810404217032643 4.296383455083264 9 76.69737198099213 4.904315994182151 7.479494987461845 10.918817037363883 10 43.76987174947659 25.349770027908967 15.345030768867607 15.535327453746827 11 34.244505818756934 24.10249147024163 23.09562589995987 18.557376811041564 12 26.63390948923135 21.860694837112938 27.91932001619701 23.586075657458704 13 26.174328468783536 23.754219483983665 30.389908465115607 19.681543582117193 14 20.932998498326732 28.477317833595716 28.84992164788213 21.739762020195418 15 18.752576177687715 23.415026538034827 34.046945901630416 23.78545138264705 16 21.92442970008298 23.730795559986127 28.897858980714304 25.446915759216587 17 22.203519399185428 25.893422961619265 28.820505557280573 23.082552081914734 18 23.1967664090035 22.541622860297174 28.601882266636885 25.659728464062436 19 23.73842195384579 24.910162896141227 26.38314639589337 24.968268754119617 20 29.346908314403535 24.349259785843596 28.137943306840697 18.165888592912175 21 25.875991204225752 28.56919772152404 26.140554438833597 19.41425663541661 22 24.124462747789707 22.1717427581035 30.308741844752042 23.395052649354753 23 25.34178047243694 25.791192967738535 26.976370889691477 21.890655670133043 24 22.5123883505018 25.188163110406574 28.484581065843017 23.814867473248608 25 22.13215814235572 29.339645082156235 26.093343429226156 22.43485334626189 26 21.665132308854425 26.509708217802547 28.50818657064674 23.316972902696293 27 24.14243924760177 25.993837147438164 27.490789313606395 22.372934291353666 28 19.36631930258444 27.972160030796108 30.345784329213267 22.315736337406193 29 21.601215865078196 24.38176275015026 30.160390326100973 23.856631058670573 30 21.712888060880413 26.999431652076648 30.048718130298756 21.238962156744183 31 25.57093544983921 24.949021188664272 25.447823663247497 24.032219698249015 32 26.0421376418827 25.861827901343514 26.324677376302613 21.771357080471166 33 25.697497271748386 24.032946021473748 26.017987394660437 24.251569312117432 34 23.70737163598859 25.622322817988845 26.90210433996285 23.768201206059715 35 23.047506986321515 25.297111594116057 28.832853052100983 22.822528367461445 36 24.02604595083881 29.290073522068422 25.824240674463745 20.859639852629016 37 22.482064355869326 24.89291271955389 28.432648955274832 24.19237396930195 38 23.197492732228234 25.7367187258838 26.231889584343378 24.83389895754459 39 23.95432153239674 25.610883227199356 26.26457412945622 24.170221110947686 40 23.056767607436825 23.69320833310636 29.75945990605009 23.49056415340673 41 23.149192237783698 24.7033423578994 27.067887616007436 25.079577788309464 42 23.943608264831976 23.358373326505895 29.4238985762249 23.274119832437233 43 24.469284698730206 24.74964546347593 26.50771082893454 24.273359008859327 44 22.775862100272555 24.151518287910896 29.22869920957875 23.843920402237803 45 22.845952291458985 23.214016585590834 27.55888211592482 26.38114900702536 46 24.024230142776986 25.521000728139033 27.462099546229567 22.992669582854415 47 20.077571320401148 26.654791281942337 31.16798221960746 22.099655178049062 48 23.03043839054037 24.803030220493575 28.895135268621562 23.271396120344495 49 22.97469308304235 22.60917092019705 31.319783773576 23.0963522231846 50 23.018454057332324 24.323293730559506 28.69140160408484 23.96685060802333 51 21.861057998725304 23.537412001401805 28.06912418129754 26.532405818575356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75.0 1 274.0 2 473.0 3 760.5 4 1048.0 5 836.0 6 624.0 7 623.5 8 623.0 9 677.0 10 731.0 11 791.5 12 852.0 13 872.0 14 892.0 15 880.0 16 868.0 17 876.5 18 885.0 19 1050.0 20 1215.0 21 1606.5 22 1998.0 23 2314.0 24 2630.0 25 2994.0 26 4190.0 27 5022.0 28 5853.0 29 6684.0 30 7335.5 31 7987.0 32 9940.0 33 11893.0 34 13218.0 35 14543.0 36 15958.0 37 17373.0 38 18384.0 39 19395.0 40 22214.0 41 25033.0 42 27401.5 43 29770.0 44 32030.5 45 34291.0 46 45023.0 47 55755.0 48 52911.5 49 50068.0 50 47198.0 51 44328.0 52 41777.5 53 39227.0 54 36692.5 55 34158.0 56 32885.0 57 31612.0 58 29864.0 59 28116.0 60 24977.5 61 21839.0 62 19621.0 63 17403.0 64 15513.5 65 13624.0 66 11439.5 67 9255.0 68 7945.5 69 6636.0 70 5377.0 71 4118.0 72 3371.5 73 2625.0 74 2206.5 75 1433.0 76 1078.0 77 792.5 78 507.0 79 352.5 80 198.0 81 137.0 82 76.0 83 59.0 84 42.0 85 22.0 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 550719.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.12688637949536 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87361345689918 22.9945557251566 2 8.804515713239335 5.481143204649646 3 2.9799028199534434 2.7826528949588596 4 1.6341011342147498 2.03457921349228 5 1.0971015908365063 1.707467828236577 6 0.88608452211687 1.6548631345536753 7 0.7682453291158498 1.673915954967695 8 0.7438998360987732 1.8524228540777388 9 0.6905676402672454 1.9345698428359295 >10 8.37000853517443 49.54113507730342 >50 0.1064307236383345 2.1448908870672527 >100 0.03843331686939856 2.1601668389830393 >500 0.0035476907879444823 0.8860052111156473 >1k 0.0029564089899537355 2.1655042197728926 >5k 5.912817979907471E-4 0.9861271128287573 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGC 5358 0.9729099595256384 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCG 4273 0.7758947848176656 No Hit GAATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC 3190 0.579242771722058 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCTGTGT 1950 0.3540825720558034 No Hit GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 1236 0.22443387644152463 TruSeq Adapter, Index 14 (95% over 21bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTA 1117 0.2028257605058115 No Hit CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 950 0.17250176587334012 TruSeq Adapter, Index 14 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT 879 0.15960952863438524 No Hit CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT 815 0.14798835703870758 TruSeq Adapter, Index 15 (95% over 22bp) GAATGATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTC 781 0.1418146096285038 No Hit GAATGACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCT 726 0.13182766528846834 No Hit TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC 663 0.12038807449897317 TruSeq Adapter, Index 14 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15942794782820277 0.0 2 0.0 0.0 0.0 0.7404865276120853 0.0 3 0.0 0.0 0.0 0.9313279549098542 0.0 4 0.0 0.0 0.0 1.4085223135573677 0.0 5 0.0 0.0 0.0 2.552299811700704 0.0 6 0.0 0.0 0.0 2.864618798334541 0.0 7 0.0 0.0 0.0 3.296962697854986 0.0 8 0.0 0.0 0.0 3.8772949544141384 0.0 9 0.0 0.0 0.0 4.085386558299241 0.0 10 0.0 0.0 0.0 5.369889181233987 0.0 11 0.0 0.0 0.0 6.189908101953991 0.0 12 0.0 0.0 0.0 8.102135571861512 0.0 13 0.0 0.0 0.0 8.446594361189645 0.0 14 0.0 0.0 0.0 8.586411581950141 0.0 15 0.0 0.0 0.0 8.987705163613386 0.0 16 0.0 0.0 0.0 9.421683290389472 0.0 17 0.0 0.0 0.0 9.921212088197429 0.0 18 0.0 0.0 0.0 10.473762481410665 0.0 19 0.0 0.0 0.0 11.297957760672865 0.0 20 0.0 0.0 0.0 11.75971775079487 0.0 21 0.0 0.0 0.0 12.362566027320648 0.0 22 0.0 0.0 0.0 12.964506399815514 0.0 23 0.0 0.0 0.0 13.532127999941894 0.0 24 0.0 0.0 0.0 13.991527439583527 0.0 25 0.0 0.0 0.0 14.414610717988666 0.0 26 0.0 0.0 0.0 14.789938244367818 0.0 27 0.0 0.0 0.0 15.18378701297758 0.0 28 0.0 0.0 0.0 15.582720044160451 0.0 29 0.0 0.0 0.0 16.03013515059404 0.0 30 0.0 0.0 0.0 16.557627392554096 0.0 31 0.0 0.0 0.0 16.995055554647653 0.0 32 0.0 0.0 0.0 17.43121265109793 0.0 33 0.0 0.0 0.0 17.88171463123662 0.0 34 0.0 0.0 0.0 18.317690146880715 0.0 35 0.0 0.0 0.0 18.820305818393773 0.0 36 0.0 0.0 0.0 19.27916051561686 0.0 37 0.0 0.0 0.0 19.73801521283994 0.0 38 0.0 0.0 0.0 20.203225238279412 0.0 39 0.0 0.0 0.0 20.721275278317982 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAG 30 2.163435E-6 45.000004 42 CGACGCC 20 7.030084E-4 45.0 21 CCGATCG 20 7.030084E-4 45.0 40 CCGATCA 45 3.8380676E-10 45.0 40 TTCGTAC 20 7.030084E-4 45.0 33 TTACTCG 25 3.8881502E-5 45.0 1 TTATCCG 20 7.030084E-4 45.0 1 CTTATCG 20 7.030084E-4 45.0 13 TCGTACA 25 3.8881502E-5 45.0 34 CGTAAGC 25 3.8881502E-5 45.0 43 CCGAATC 20 7.030084E-4 45.0 19 CGATCGA 20 7.030084E-4 45.0 41 CGATCAA 40 6.8048394E-9 45.0 41 ATCGACG 20 7.030084E-4 45.0 16 TCGAATG 140 0.0 41.785717 44 GCGAGAC 220 0.0 40.909092 21 TTGCCCG 50 1.0786607E-9 40.5 1 ACATACG 50 1.0786607E-9 40.5 17 TTCGTTG 245 0.0 40.408165 1 GCGCGAC 90 0.0 40.0 9 >>END_MODULE