##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934040.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118079
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.08989078589259	31.0	30.0	31.0	26.0	34.0
2	30.355906872412415	31.0	30.0	33.0	27.0	34.0
3	30.38523038175299	31.0	30.0	34.0	26.0	34.0
4	34.05300967105187	35.0	33.0	37.0	30.0	37.0
5	34.44211097784682	35.0	35.0	37.0	32.0	37.0
6	33.944530753193646	35.0	35.0	37.0	30.0	37.0
7	34.34660073214862	35.0	35.0	37.0	32.0	37.0
8	34.52700658897985	35.0	35.0	37.0	32.0	37.0
9	35.90415435760801	37.0	35.0	39.0	31.0	39.0
10	35.27390551114903	37.0	34.0	39.0	30.0	39.0
11	35.42466856098719	37.0	35.0	39.0	30.0	39.0
12	35.51087534959515	37.0	35.0	39.0	30.0	39.0
13	35.5567746107386	37.0	35.0	39.0	30.0	39.0
14	36.34119950379177	38.0	35.0	40.0	30.0	41.0
15	36.58279871100343	38.0	35.0	40.0	31.0	41.0
16	36.70710477524397	38.0	35.0	40.0	31.0	41.0
17	36.42505851554318	38.0	35.0	40.0	30.0	41.0
18	36.536795700482706	38.0	35.0	40.0	30.0	41.0
19	36.54341508963141	38.0	35.0	40.0	30.0	41.0
20	36.56152382792271	38.0	35.0	40.0	30.0	41.0
21	36.24581089529452	38.0	35.0	40.0	30.0	41.0
22	36.40959449198134	38.0	35.0	40.0	30.0	41.0
23	36.44704354522355	38.0	35.0	40.0	30.0	41.0
24	36.37625606061826	38.0	35.0	40.0	30.0	41.0
25	35.893264250558325	38.0	34.0	40.0	29.0	41.0
26	36.01981344788695	38.0	34.0	40.0	30.0	41.0
27	36.04659420309299	38.0	34.0	40.0	30.0	41.0
28	35.923673550795606	38.0	34.0	40.0	29.0	41.0
29	36.06157972737168	38.0	34.0	40.0	30.0	41.0
30	35.59806865167846	38.0	34.0	40.0	28.0	41.0
31	35.79641420686731	38.0	34.0	40.0	29.0	41.0
32	35.798578633531264	38.0	34.0	40.0	29.0	41.0
33	35.804673015055286	38.0	34.0	40.0	29.0	41.0
34	35.7941344037407	38.0	34.0	40.0	29.0	41.0
35	35.782882962652906	38.0	34.0	40.0	29.0	41.0
36	35.580884713870844	38.0	34.0	40.0	28.0	41.0
37	35.6901667950118	38.0	34.0	40.0	29.0	41.0
38	35.59684065258358	38.0	34.0	40.0	28.0	41.0
39	35.49298305397025	38.0	34.0	40.0	28.0	41.0
40	35.49082578243577	38.0	34.0	40.0	28.0	41.0
41	35.33633312136262	38.0	34.0	40.0	27.0	41.0
42	35.49335869826729	38.0	34.0	40.0	28.0	41.0
43	35.47651015715348	38.0	34.0	40.0	28.0	41.0
44	35.4366864953192	38.0	34.0	40.0	28.0	41.0
45	35.487210653272264	38.0	34.0	40.0	28.0	41.0
46	35.39922939255634	38.0	34.0	40.0	28.0	41.0
47	35.33144527354507	37.0	34.0	40.0	28.0	41.0
48	35.27771651198171	37.0	34.0	40.0	28.0	41.0
49	35.27358531910536	37.0	34.0	40.0	28.0	41.0
50	35.09825602663139	37.0	34.0	40.0	27.0	41.0
51	33.988871984895525	36.0	32.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	11.0
11	14.0
12	22.0
13	30.0
14	41.0
15	63.0
16	144.0
17	300.0
18	524.0
19	989.0
20	1739.0
21	2841.0
22	4476.0
23	6303.0
24	8481.0
25	10879.0
26	13466.0
27	16462.0
28	20663.0
29	25656.0
30	32760.0
31	41851.0
32	53388.0
33	69794.0
34	94317.0
35	103485.0
36	126850.0
37	165089.0
38	206742.0
39	110692.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.18147554868663	4.4773222643480475	53.87186415271192	17.469338034253393
2	19.30436042533667	5.552738223327689	54.99870760473991	20.14419374659572
3	20.925891640930562	5.800484581143193	51.86431370234125	21.409310075585
4	19.379399845628082	5.08738649057893	48.347478129899585	27.1857355338934
5	19.80718714867196	5.899940880742774	50.09556569795157	24.197306272633686
6	22.02652943128348	7.916971877658019	52.23011969637208	17.826378994686422
7	85.3795662023882	2.383373625656148	8.746788017662437	3.490272154293212
8	87.80211416187944	4.047746178937266	4.943300070925221	3.206839588258075
9	82.58092675025647	5.035958997530586	6.486661497085626	5.896452755127321
10	41.33759779049602	27.598139308581953	15.625729487809	15.438533413113026
11	30.21566454606517	27.025281755582565	23.86817031712428	18.890883381227983
12	26.7680548512225	23.61443153837967	27.762170651626583	21.85534295877125
13	25.1387424323326	23.38358917393136	30.27889800273505	21.19877039100099
14	21.238570798664494	26.32971373221391	29.783315847985698	22.648399621135894
15	20.209305424750845	26.093952216256632	32.11839234973557	21.57835000925695
16	23.15632437421685	25.133286646113557	29.4549848445414	22.2554041351282
17	23.030572973823855	26.353862294167048	28.676596197585326	21.938968534423775
18	22.643391030508578	25.25796477708641	29.01610709082274	23.08253710158227
19	24.169937902420134	26.090285212404492	27.699652707903468	22.04012417727191
20	26.863486390496554	26.251722821017122	27.690619356950624	19.194171431535697
21	26.028572220746476	27.437685530271118	26.96660969394828	19.567132555034124
22	24.98186621875556	24.448719634301334	28.519183349298217	22.05023079764489
23	24.36983433192109	26.059070960102105	27.59250464412622	21.978590063850586
24	22.75733646728004	26.019807187148675	29.083991381646555	22.138864963924732
25	22.46084578996654	27.688920013702074	27.00694673632185	22.843287460009535
26	22.442421331587482	27.57059206013171	27.223478841834968	22.76350776644584
27	22.483921082499535	27.630695147659512	27.60592051187796	22.279463257962988
28	20.630205915682165	27.460224188094045	29.69405560787744	22.215514288346352
29	21.318887126938257	25.628600483507874	29.29721423978091	23.75529814977296
30	21.92769920551231	26.775925493636855	28.690727578283827	22.60564772256701
31	23.309175827468362	26.74506899780785	26.786926505193282	23.158828669530507
32	23.638401222096114	27.733013499046134	26.137956262482348	22.490629016375408
33	23.17385444141246	27.042275188068103	26.705536907499383	23.078333463020055
34	22.279016062371262	26.564938613461127	27.377403564506626	23.77864175966099
35	22.118293966705394	26.77046970741781	26.91652378767511	24.194712538201685
36	21.568064510647282	28.04703424355524	26.75714327878442	23.627757967013064
37	21.841569334546126	28.04828639121207	27.302632461570248	22.807511812671557
38	21.75248797267456	27.135560188501884	27.449849250366032	23.662102588457522
39	21.760537493325604	28.365884700454973	27.139316631472372	22.734261174747044
40	21.984045850069627	26.03930491494787	27.75778813482768	24.21886110015482
41	21.525133733841706	26.249129086585114	27.52479923153909	24.70093794803408
42	21.415213057395764	26.04601284882374	27.596708282688432	24.94206581109206
43	21.422815382455084	26.68272993232142	27.763780555756796	24.1306741294667
44	21.686392464217644	26.0680148719366	28.095241928343167	24.15035073550259
45	21.519499069385972	25.848978471109824	27.04433228779004	25.587190171714163
46	21.722078672437277	26.980383318173402	27.122859833696904	24.174678175692417
47	20.147145237501107	26.976626875202914	29.151428476878642	23.724799410417333
48	20.998963400618383	25.914000710146603	28.822560838724275	24.264475050510743
49	21.30180425533437	25.493368536570316	29.359642744385685	23.845184463709632
50	21.065148348193645	26.438203382766336	28.00446122322305	24.492187045816976
51	20.721165499038978	25.406433713539023	27.658421274346445	26.213979513075554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	455.0
2	731.0
3	2008.0
4	3285.0
5	2468.5
6	1652.0
7	1601.0
8	1550.0
9	1590.5
10	1631.0
11	1658.5
12	1686.0
13	1684.5
14	1683.0
15	1742.0
16	1801.0
17	1901.5
18	2002.0
19	2228.5
20	2455.0
21	3104.5
22	3754.0
23	4349.0
24	4944.0
25	6085.5
26	9879.5
27	12532.0
28	14284.5
29	16037.0
30	19370.0
31	22703.0
32	25872.0
33	29041.0
34	32807.0
35	36573.0
36	38585.5
37	40598.0
38	45268.0
39	49938.0
40	53392.0
41	56846.0
42	61661.5
43	66477.0
44	69261.0
45	72045.0
46	74357.0
47	76669.0
48	85228.0
49	93787.0
50	92363.0
51	90939.0
52	88417.0
53	85895.0
54	79999.5
55	74104.0
56	68495.0
57	62886.0
58	58048.0
59	53210.0
60	48324.5
61	43439.0
62	38410.5
63	33382.0
64	28803.0
65	24224.0
66	19768.5
67	15313.0
68	12790.5
69	10268.0
70	8455.5
71	6643.0
72	5707.0
73	4771.0
74	3773.0
75	2073.5
76	1372.0
77	989.0
78	606.0
79	411.5
80	217.0
81	178.0
82	139.0
83	96.0
84	53.0
85	29.5
86	6.0
87	7.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1118079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.21251071175226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.16084786233263	21.66424562571022
2	8.111181955921717	4.738959795446696
3	2.673807803068294	2.3432591726489793
4	1.474609620808684	1.7230819737410648
5	1.0373712153794852	1.5152108870668337
6	0.8643410130791402	1.5149742661908712
7	0.7323794952738393	1.4976250695528321
8	0.6916167603954689	1.6163089617184025
9	0.6173219454490629	1.6230171549627446
>10	9.47236505525711	56.39125189066372
>50	0.13020465011577168	2.4126471680955595
>100	0.029903227525158033	1.4229738714182631
>500	0.002180443673527712	0.43249743000955193
>1k	0.0018689517201666101	1.1039467327742236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	3469	0.3102643015386211	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2372	0.2121495887142143	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	2298	0.2055310939566882	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1672	0.14954220587275138	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTACGCAT	1178	0.10535928141034757	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1143	0.10222891226827442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07477110293637569	0.0
2	0.0	0.0	0.0	0.3371854761604502	0.0
3	0.0	0.0	0.0	0.4486266176182542	0.0
4	0.0	0.0	0.0	0.7025442745995587	0.0
5	0.0	0.0	0.0	1.2466918706102164	0.0
6	0.0	0.0	0.0	1.5555251462553183	0.0
7	0.0	0.0	0.0	1.8291194092725112	0.0
8	0.0	0.0	0.0	2.2338314197833964	0.0
9	0.0	0.0	0.0	2.4107419958697016	0.0
10	0.0	0.0	0.0	2.9497915621346973	0.0
11	0.0	0.0	0.0	3.540358060566382	0.0
12	0.0	0.0	0.0	4.4889493497328905	0.0
13	0.0	0.0	0.0	4.718181810050989	0.0
14	0.0	0.0	0.0	4.807978684869316	0.0
15	0.0	0.0	0.0	4.994727563973566	0.0
16	0.0	0.0	0.0	5.247124755943005	0.0
17	8.9439118344947E-5	0.0	0.0	5.586993405653804	0.0
18	8.9439118344947E-5	0.0	0.0	5.94922183495084	0.0
19	8.9439118344947E-5	0.0	0.0	6.366008126438293	0.0
20	8.9439118344947E-5	0.0	0.0	6.708291632344405	0.0
21	8.9439118344947E-5	0.0	0.0	7.1785625166021365	0.0
22	8.9439118344947E-5	0.0	0.0	7.688812686760059	0.0
23	8.9439118344947E-5	0.0	0.0	8.172588877887877	0.0
24	8.9439118344947E-5	0.0	0.0	8.561827920925086	0.0
25	8.9439118344947E-5	0.0	0.0	8.929422697322819	0.0
26	8.9439118344947E-5	0.0	0.0	9.243890637423652	0.0
27	8.9439118344947E-5	0.0	0.0	9.556569795157587	0.0
28	8.9439118344947E-5	0.0	0.0	9.887583972152235	0.0
29	8.9439118344947E-5	0.0	0.0	10.257325287390247	0.0
30	8.9439118344947E-5	0.0	0.0	10.652467312238223	0.0
31	8.9439118344947E-5	0.0	0.0	11.035624495227976	0.0
32	8.9439118344947E-5	0.0	0.0	11.412431500815238	0.0
33	8.9439118344947E-5	0.0	0.0	11.791742801716158	0.0
34	8.9439118344947E-5	0.0	0.0	12.178924745031434	0.0
35	8.9439118344947E-5	0.0	0.0	12.589003102643016	0.0
36	8.9439118344947E-5	0.0	0.0	13.008114811207436	0.0
37	8.9439118344947E-5	0.0	0.0	13.418372047055708	0.0
38	8.9439118344947E-5	0.0	0.0	13.841240198590619	0.0
39	8.9439118344947E-5	0.0	0.0	14.272247309894919	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	20	7.0332026E-4	45.000004	2
CGATCAT	20	7.0332026E-4	45.000004	10
TATCGCG	20	7.0332026E-4	45.000004	1
CGCGCAT	45	1.9283107E-8	40.0	36
TACGCGC	45	1.9283107E-8	40.0	34
CGTCATA	40	3.458099E-7	39.375004	38
ACGGGTA	35	6.248136E-6	38.571426	5
TCGATAA	35	6.248136E-6	38.571426	20
TCGTACG	35	6.248136E-6	38.571426	29
CGATGAA	895	0.0	38.463684	19
CGTTTTT	1430	0.0	38.39161	1
TGGGCGA	1460	0.0	38.219177	6
TTTGGGC	2785	0.0	38.13286	4
TTTACGG	95	0.0	37.894737	2
TTTGTCG	345	0.0	37.826088	1
CCGATGA	920	0.0	37.66304	18
TATTGCG	30	1.1399022E-4	37.499996	1
TCGCGTA	30	1.1399022E-4	37.499996	12
TTTGGGA	5525	0.0	37.34389	4
TTGGGAC	2270	0.0	37.070484	5
>>END_MODULE