FastQCFastQC Report
Sat 14 Jan 2017
SRR2934039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629529
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC16720.265595389569027Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT10430.16567942064622915Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC9560.1518595648492762Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC9090.1443936657405775Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8480.13470388179098977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT302.1639316E-645.00000412
GTCGCAC207.0308533E-445.024
AACGGTC207.0308533E-445.015
ACGGATA207.0308533E-445.029
AGCTACG502.1827873E-1145.09
CGATAGG253.8887883E-545.010
CGTTTGG3400.041.029412
CTTACGG501.0804797E-940.52
ACATACG1400.040.17857417
GCGATAT451.925946E-840.09
GTTTGCG1300.039.8076931
GCGATAG403.4548975E-739.3759
TCATCGA403.4548975E-739.37516
GGGCGAT13900.039.010797
GCGACTA356.2437866E-638.5714320
GCGACAC356.2437866E-638.5714311
GGGTACG700.038.571437
CGCGCAA356.2437866E-638.5714316
TGGGCGA10300.038.44666
CACGACC1000.038.2527
Produced by FastQC (version 0.11.5)