##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	629529
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.09490428558494	31.0	30.0	31.0	26.0	34.0
2	30.482556006156983	31.0	30.0	33.0	27.0	34.0
3	30.565338530869905	31.0	30.0	34.0	27.0	34.0
4	34.11287962905601	35.0	33.0	37.0	30.0	37.0
5	34.44386040992551	35.0	35.0	37.0	32.0	37.0
6	33.972114072584425	35.0	35.0	37.0	30.0	37.0
7	34.400583610921814	35.0	35.0	37.0	32.0	37.0
8	34.67528263193594	35.0	35.0	37.0	32.0	37.0
9	35.92850686783294	37.0	35.0	39.0	31.0	39.0
10	35.319661207029384	37.0	34.0	39.0	30.0	39.0
11	35.432094470628044	37.0	35.0	39.0	30.0	39.0
12	35.37041343607681	37.0	35.0	39.0	30.0	39.0
13	35.3308902369867	37.0	35.0	39.0	30.0	39.0
14	35.88007700995506	38.0	34.0	40.0	27.0	41.0
15	36.19635950051546	38.0	35.0	40.0	30.0	41.0
16	36.36489025922555	38.0	35.0	40.0	30.0	41.0
17	36.1880612330806	38.0	34.0	40.0	30.0	41.0
18	36.305199601606915	38.0	35.0	40.0	30.0	41.0
19	36.28875715018689	38.0	35.0	40.0	30.0	41.0
20	36.34117252739747	38.0	35.0	40.0	30.0	41.0
21	36.00737376673672	38.0	34.0	40.0	29.0	41.0
22	36.23273272557738	38.0	34.0	40.0	30.0	41.0
23	36.363678241987266	38.0	35.0	40.0	30.0	41.0
24	36.27215743833882	38.0	35.0	40.0	30.0	41.0
25	35.764905191023765	38.0	34.0	40.0	29.0	41.0
26	35.892607012544296	38.0	34.0	40.0	30.0	41.0
27	35.96352193465273	38.0	34.0	40.0	30.0	41.0
28	35.86393478298855	38.0	34.0	40.0	29.0	41.0
29	35.977978774607685	38.0	34.0	40.0	30.0	41.0
30	35.489853525413444	38.0	34.0	40.0	28.0	41.0
31	35.64332858375071	38.0	34.0	40.0	29.0	41.0
32	35.73146431697349	38.0	34.0	40.0	29.0	41.0
33	35.75575867037102	38.0	34.0	40.0	29.0	41.0
34	35.71343178789222	38.0	34.0	40.0	29.0	41.0
35	35.712288075688335	38.0	34.0	40.0	29.0	41.0
36	35.458210821105936	38.0	34.0	40.0	28.0	41.0
37	35.542713679592204	38.0	34.0	40.0	29.0	41.0
38	35.44902776520224	38.0	34.0	40.0	28.0	41.0
39	35.39886486563764	38.0	34.0	40.0	28.0	41.0
40	35.297519256459985	37.0	34.0	40.0	28.0	41.0
41	35.12865014955626	37.0	34.0	40.0	27.0	41.0
42	35.373042385656575	37.0	34.0	40.0	28.0	41.0
43	35.34452900501804	37.0	34.0	40.0	28.0	41.0
44	35.29104934006217	37.0	34.0	40.0	28.0	41.0
45	35.35151200341843	37.0	34.0	40.0	28.0	41.0
46	35.246855982806196	37.0	34.0	40.0	28.0	41.0
47	35.207258124724994	37.0	34.0	40.0	28.0	41.0
48	35.1536037259602	37.0	34.0	40.0	28.0	41.0
49	35.15118445695115	37.0	34.0	40.0	28.0	41.0
50	34.99384936992577	36.0	34.0	40.0	28.0	41.0
51	33.840363192164304	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	13.0
12	11.0
13	9.0
14	23.0
15	49.0
16	96.0
17	167.0
18	340.0
19	570.0
20	917.0
21	1384.0
22	2142.0
23	2892.0
24	4160.0
25	5608.0
26	7523.0
27	9549.0
28	12139.0
29	15462.0
30	19543.0
31	25394.0
32	32538.0
33	43111.0
34	58193.0
35	59470.0
36	71575.0
37	92313.0
38	110538.0
39	53793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.890718298918713	4.7993023355556295	54.57191011057473	16.738069254950926
2	20.579512619752226	3.670045383135646	53.796727394607714	21.953714602504412
3	22.823253575292004	3.2913495645156936	51.93771851654173	21.94767834365057
4	20.864328728303224	5.092696285635769	45.7788282986169	28.264146687444104
5	18.985781433420858	5.9507981363845035	49.3327551232747	25.730665306919935
6	23.13380320843043	6.503115821511003	51.67037578888344	18.692705181175132
7	85.76824896073096	2.6615136077924926	7.636661694695558	3.93357573678099
8	87.5336958265624	2.516643395300296	5.702199580956557	4.247461197180749
9	81.40323956481751	5.526194980691914	7.995660247581922	5.0749052069086575
10	42.58390002684547	26.744280247613695	15.259662382511369	15.41215734302947
11	32.224250193398554	24.636196267368142	23.89484837076608	19.24470516846722
12	29.108428682395886	22.26712351615255	28.61917401740031	20.00527378405125
13	26.104119111272077	23.7004172961055	30.005607366777387	20.189856225845038
14	20.695948876064485	24.681944755523574	33.08854715191834	21.5335592164936
15	18.94829308896016	23.766816143497756	35.48255918313533	21.802331584406755
16	24.98026302203711	23.17780435849659	30.106794127037833	21.735138492428465
17	24.73833612113183	23.444035143734443	27.417640807651434	24.399987927482293
18	26.428012053455834	22.666151996174918	29.287927958839067	21.617907991530174
19	26.104595658023698	24.442559437293596	27.185244841778538	22.267600062904172
20	26.762865570926834	26.86246384201522	26.952531178071226	19.42213940898672
21	26.834982979338523	25.036813236562573	28.476368840831796	19.65183494326711
22	25.262537547912807	22.80911602166064	29.439787523688345	22.488558906738213
23	23.854659594712874	24.318339584038227	28.959428398056325	22.867572423192577
24	23.698828806933435	24.993924028916854	28.6889086920539	22.618338472095807
25	24.148530091544632	25.75179221290838	26.42261119027082	23.677066505276166
26	22.583232861393203	26.629114782639085	25.77593724832375	25.011715107643965
27	21.83918453319863	25.55132487939396	29.62881773516391	22.980672852243504
28	19.79384587524959	26.14700831891779	29.269342635525923	24.78980317030669
29	21.847285827976155	26.05392285343487	28.591852003640817	23.506939314948163
30	23.1855879554397	25.30765064039941	29.61420363478092	21.892557769379962
31	24.254482319321273	26.27170471892478	28.057166548324226	21.416646413429724
32	25.456015529070147	26.032954796363633	26.456446009635776	22.054583664930448
33	24.52611396774414	26.123498679171252	26.464229606578886	22.88615774650572
34	21.650313170640274	24.757715689031006	30.43894721291632	23.1530239274124
35	22.318590565327412	26.987795637691036	27.737244829070622	22.95636896791093
36	24.286887498431366	27.658138068301856	26.04089724222395	22.014077191042826
37	21.986755177283335	27.686095477730177	28.039693167431523	22.287456177554965
38	22.562105955404753	26.93426355259249	27.84208511442682	22.661545377575933
39	21.475102814961662	26.057417529613407	27.68847821148827	24.779001443936657
40	24.61157468520116	24.95151136802276	28.294645679547724	22.142268267228356
41	21.807891296508977	24.57837526150503	27.450204835678736	26.163528606307256
42	22.08857733321261	24.986140431973748	28.10577431698937	24.81950791782428
43	21.80264928224117	24.502763176914804	29.204055730554114	24.490531810289916
44	22.648837464199424	24.983122302546825	28.182021797248417	24.18601843600533
45	21.963563235371204	24.99265323757921	27.092318225212818	25.95146530183677
46	22.52429991310964	25.102576688286003	28.299887693815535	24.073235704788818
47	19.85484385945683	26.348428745935454	30.926295690905427	22.870431703702295
48	21.143585124751997	26.4494566572787	29.713801905869307	22.693156312099998
49	22.65693875897695	24.516583032711758	29.780359602178773	23.04611860613252
50	21.018253329076185	25.477460132893004	28.600429845169963	24.903856692860852
51	20.768383982310585	25.293195388933633	27.084216930435296	26.85420369832049
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	249.5
2	415.0
3	744.5
4	1074.0
5	850.0
6	626.0
7	644.5
8	663.0
9	691.5
10	720.0
11	755.5
12	791.0
13	840.5
14	890.0
15	864.0
16	838.0
17	834.0
18	830.0
19	991.5
20	1153.0
21	1553.0
22	1953.0
23	2230.0
24	2507.0
25	3244.0
26	4712.0
27	5443.0
28	7056.0
29	8669.0
30	9393.5
31	10118.0
32	12333.5
33	14549.0
34	16001.5
35	17454.0
36	18611.0
37	19768.0
38	22456.5
39	25145.0
40	28794.0
41	32443.0
42	35017.5
43	37592.0
44	41996.5
45	46401.0
46	50174.0
47	53947.0
48	54079.0
49	54211.0
50	49833.5
51	45456.0
52	42910.5
53	40365.0
54	39175.0
55	37985.0
56	37376.0
57	36767.0
58	35416.5
59	34066.0
60	30738.0
61	27410.0
62	24040.5
63	20671.0
64	17712.5
65	14754.0
66	12722.5
67	10691.0
68	8903.0
69	7115.0
70	6092.0
71	5069.0
72	4108.5
73	3148.0
74	2401.0
75	1445.0
76	1236.0
77	890.5
78	545.0
79	384.0
80	223.0
81	152.0
82	81.0
83	46.0
84	11.0
85	11.5
86	12.0
87	8.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.30541180251297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75502185295971	23.82686353463207
2	9.401007567516148	6.074068416543
3	3.362463054561468	3.258772609450316
4	1.7058547993000988	2.2043336706673125
5	1.1227980693383843	1.8136227000521514
6	0.8016312557823532	1.5538216699088716
7	0.6626348171553721	1.4984683450021028
8	0.6346147155280503	1.6401191776854631
9	0.5371542562293822	1.5617690504066486
>10	7.854815948188544	50.77352437416024
>50	0.11714111048782996	2.5264576280442568
>100	0.042370188899760464	2.3940879314213297
>500	0.0014954184317562515	0.436884412318966
>1k	9.969456211708343E-4	0.43720647970733234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1672	0.265595389569027	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1043	0.16567942064622915	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	956	0.1518595648492762	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	909	0.1443936657405775	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	848	0.13470388179098977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1825174058701029	0.0
2	0.0	0.0	0.0	0.8040932188985733	0.0
3	0.0	0.0	0.0	1.0746129249009975	0.0
4	0.0	0.0	0.0	1.4809484551148557	0.0
5	0.0	0.0	0.0	2.109037073748787	0.0
6	0.0	0.0	0.0	2.5015527481656923	0.0
7	0.0	0.0	0.0	2.870241085001644	0.0
8	0.0	0.0	0.0	3.486257185927892	0.0
9	0.0	0.0	0.0	3.780921927345682	0.0
10	0.0	0.0	0.0	4.298134001769577	0.0
11	0.0	0.0	0.0	5.394032681576226	0.0
12	0.0	0.0	0.0	6.304872372837471	0.0
13	0.0	0.0	0.0	6.638613947888024	0.0
14	0.0	0.0	0.0	6.759815671716474	0.0
15	0.0	0.0	0.0	6.946145451599529	0.0
16	0.0	0.0	0.0	7.372972492133007	0.0
17	0.0	0.0	0.0	7.993595211658239	0.0
18	0.0	0.0	0.0	8.69840785730284	0.0
19	0.0	0.0	0.0	9.18750367338121	0.0
20	0.0	0.0	0.0	9.73982135850771	0.0
21	0.0	0.0	0.0	10.457818464280438	0.0
22	0.0	0.0	0.0	11.243802906617487	0.0
23	0.0	0.0	0.0	11.967836271244057	0.0
24	0.0	0.0	0.0	12.527619855479255	0.0
25	0.0	0.0	0.0	13.058492936782896	0.0
26	0.0	0.0	0.0	13.539646307000949	0.0
27	0.0	0.0	0.0	14.056699532507636	0.0
28	0.0	0.0	0.0	14.548495780178515	0.0
29	0.0	0.0	0.0	15.120510731038602	0.0
30	0.0	0.0	0.0	15.810709276300217	0.0
31	0.0	0.0	0.0	16.351272141553448	0.0
32	0.0	0.0	0.0	16.875632417251627	0.0
33	0.0	0.0	0.0	17.432080174225494	0.0
34	0.0	0.0	0.0	17.996629225976882	0.0
35	0.0	0.0	0.0	18.67666144053729	0.0
36	0.0	0.0	0.0	19.229137974580997	0.0
37	0.0	0.0	0.0	19.817355514996134	0.0
38	0.0	0.0	0.0	20.504218232996415	0.0
39	0.0	0.0	0.0	21.529905691397854	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACT	30	2.1639316E-6	45.000004	12
GTCGCAC	20	7.0308533E-4	45.0	24
AACGGTC	20	7.0308533E-4	45.0	15
ACGGATA	20	7.0308533E-4	45.0	29
AGCTACG	50	2.1827873E-11	45.0	9
CGATAGG	25	3.8887883E-5	45.0	10
CGTTTGG	340	0.0	41.02941	2
CTTACGG	50	1.0804797E-9	40.5	2
ACATACG	140	0.0	40.178574	17
GCGATAT	45	1.925946E-8	40.0	9
GTTTGCG	130	0.0	39.807693	1
GCGATAG	40	3.4548975E-7	39.375	9
TCATCGA	40	3.4548975E-7	39.375	16
GGGCGAT	1390	0.0	39.01079	7
GCGACTA	35	6.2437866E-6	38.57143	20
GCGACAC	35	6.2437866E-6	38.57143	11
GGGTACG	70	0.0	38.57143	7
CGCGCAA	35	6.2437866E-6	38.57143	16
TGGGCGA	1030	0.0	38.4466	6
CACGACC	100	0.0	38.25	27
>>END_MODULE