##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390716
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.020884734692206	31.0	30.0	31.0	26.0	34.0
2	30.426199592542922	31.0	30.0	33.0	27.0	34.0
3	30.418329425976925	31.0	30.0	34.0	26.0	34.0
4	34.054231206298184	35.0	33.0	37.0	30.0	37.0
5	34.44922654818333	35.0	35.0	37.0	32.0	37.0
6	34.05429263198845	35.0	35.0	37.0	30.0	37.0
7	34.47214089005825	35.0	35.0	37.0	32.0	37.0
8	34.63116432395909	35.0	35.0	37.0	32.0	37.0
9	35.877842217876925	37.0	35.0	39.0	30.0	39.0
10	35.29750253380973	37.0	34.0	39.0	30.0	39.0
11	35.45913911895085	37.0	35.0	39.0	30.0	39.0
12	35.40941758208008	37.0	35.0	39.0	30.0	39.0
13	35.35513774711043	37.0	35.0	39.0	30.0	39.0
14	36.032102601377986	38.0	34.0	40.0	29.0	41.0
15	36.326469865580115	38.0	35.0	40.0	30.0	41.0
16	36.44544886823166	38.0	35.0	40.0	31.0	41.0
17	36.24858209031624	38.0	35.0	40.0	30.0	41.0
18	36.346550947491274	38.0	35.0	40.0	30.0	41.0
19	36.36220170149162	38.0	35.0	40.0	30.0	41.0
20	36.45486747407324	38.0	35.0	40.0	30.0	41.0
21	36.120737313035555	38.0	34.0	40.0	30.0	41.0
22	36.29100676706354	38.0	34.0	40.0	30.0	41.0
23	36.424625559229725	38.0	35.0	40.0	30.0	41.0
24	36.316526581967466	38.0	35.0	40.0	30.0	41.0
25	35.84570634425005	38.0	34.0	40.0	29.0	41.0
26	35.92337401079045	38.0	34.0	40.0	30.0	41.0
27	35.995029637895556	38.0	34.0	40.0	30.0	41.0
28	35.87323017229906	38.0	34.0	40.0	29.0	41.0
29	36.02144780351969	38.0	34.0	40.0	30.0	41.0
30	35.51483942300801	38.0	34.0	40.0	28.0	41.0
31	35.66280623266004	38.0	34.0	40.0	29.0	41.0
32	35.77256114415586	38.0	34.0	40.0	29.0	41.0
33	35.78286530369885	38.0	34.0	40.0	29.0	41.0
34	35.74124172032883	38.0	34.0	40.0	29.0	41.0
35	35.81582786474063	38.0	34.0	40.0	29.0	41.0
36	35.60364561471759	38.0	34.0	40.0	29.0	41.0
37	35.704289048823185	38.0	34.0	40.0	29.0	41.0
38	35.58667932718394	38.0	34.0	40.0	29.0	41.0
39	35.54753324665486	38.0	34.0	40.0	29.0	41.0
40	35.42689063155847	38.0	34.0	40.0	28.0	41.0
41	35.3350745810256	37.0	34.0	40.0	28.0	41.0
42	35.60719806713828	38.0	34.0	40.0	29.0	41.0
43	35.57406914485202	38.0	34.0	40.0	29.0	41.0
44	35.464506188638296	37.0	34.0	40.0	29.0	41.0
45	35.54472814013248	37.0	34.0	40.0	29.0	41.0
46	35.4188336285179	37.0	34.0	40.0	29.0	41.0
47	35.40471083856305	37.0	34.0	40.0	29.0	41.0
48	35.41012909632572	37.0	34.0	40.0	29.0	41.0
49	35.417991584680436	37.0	34.0	40.0	28.0	41.0
50	35.24094994830004	37.0	34.0	40.0	28.0	41.0
51	34.09894654941185	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	3.0
13	9.0
14	14.0
15	21.0
16	46.0
17	87.0
18	125.0
19	241.0
20	431.0
21	711.0
22	1072.0
23	1584.0
24	2277.0
25	3209.0
26	4316.0
27	5494.0
28	7099.0
29	9482.0
30	11888.0
31	15382.0
32	20023.0
33	26400.0
34	37508.0
35	38221.0
36	45247.0
37	58411.0
38	69306.0
39	32104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.541354845975082	4.73720042178974	53.05260086610223	17.668843866132946
2	19.969491907165306	3.6617389612915776	53.97680156430758	22.39196756723554
3	22.300596852957135	3.8849189692769173	51.843282588888094	21.971201588877857
4	20.787477349276713	5.50553343093193	45.49519343973628	28.211795780055077
5	17.87666745155049	6.215767974692615	49.22091749506035	26.686647078696545
6	22.316977037029453	5.899425669795964	52.676112572815036	19.107484720359544
7	83.6489931305603	2.7273006480410324	9.119667482263331	4.504038739135331
8	84.53377901084164	2.9614860921999613	6.8894030446667145	5.61533185229169
9	77.70170661042803	6.248016462084992	9.985257834334913	6.06501909315206
10	39.08286325617584	28.639216208192142	17.135208181901945	15.142712353730076
11	31.648051269976147	25.117988513395918	24.99616089435805	18.237799322269886
12	29.641478721117124	21.6855721291168	29.389633391005233	19.28331575876084
13	24.23217887161007	24.09473888962827	30.321512300494476	21.351569938267183
14	19.285107341393747	27.28964260485877	30.52959182628815	22.895658227459332
15	18.088586082986108	25.24723840334156	36.13673358654368	20.527441927128656
16	21.4874742779922	24.255725386214028	32.89396902097687	21.362831314816898
17	21.1289018110341	24.32585304927364	28.666089947685787	25.87915519200647
18	22.01906243921416	24.55056869951576	30.48429037971314	22.946078481556935
19	23.746148097339244	26.12972082023772	28.674024099345818	21.450106983077223
20	27.462146418370377	25.739155806263376	28.677863204987762	18.120834570378484
21	25.90782051413303	25.717400874292323	27.973259349501937	20.401519262072707
22	21.98962929595921	25.323508635428286	28.40886986967516	24.277992198937334
23	22.494343717687528	25.499083733453453	28.808392796814054	23.198179752044965
24	23.860809385845474	23.279824732030427	29.796322648675766	23.063043233448337
25	21.711166166729797	25.169944409750304	27.49874589215696	25.62014353136293
26	19.509055170507477	28.996508973269584	27.498234011404705	23.99620184481823
27	20.215450608626213	27.8593658821241	28.458266362268247	23.46691714698144
28	17.814729880527032	28.925101608329324	30.019246716284975	23.24092179485867
29	20.02016810163904	26.74141831918836	28.2051413302757	25.0332722488969
30	20.225688223671415	27.49951371328535	28.65380480963155	23.620993253411683
31	21.47058221316762	29.828059255315882	25.56460446974273	23.13675406177377
32	22.485385804522977	28.460057944901155	25.061681630647325	23.992874619928543
33	22.255295406382128	27.66536307701758	26.54741551408184	23.531926002518453
34	19.191177223354046	27.918744049386255	28.39120998372219	24.498868743537507
35	18.230632991738243	27.565034449574625	26.963318625293052	27.241013933394076
36	19.768578711903277	29.837784989608824	27.07849179455154	23.315144503936363
37	20.215962489378477	29.277787446636435	26.949753785358162	23.55649627862693
38	18.96287840784611	29.671423745124336	25.60350740691448	25.762190440115067
39	20.775192211222475	27.073628927405068	28.039547906919605	24.11163095445285
40	21.399686728979617	27.244853039036027	27.627995782102598	23.727464449881754
41	17.265737773728233	28.028542470746014	27.831468381125934	26.874251374399822
42	19.9549033057259	25.814914157597848	27.77234615423991	26.457836382436344
43	20.399727679439795	25.558461900715606	28.058231554377095	25.9835788654675
44	19.599914004033618	26.497763081112623	27.387411828540426	26.51491108631333
45	20.58656415401468	26.914689953828358	24.70694826933118	27.791797622825786
46	19.881960298528856	28.034429099397006	26.80437965171634	25.279230950357807
47	17.04358152724741	27.955599463548968	29.345099765558615	25.655719243645002
48	18.011548029770985	25.687967731037375	29.757675651880138	26.5428085873115
49	19.456331453024703	24.99078614645932	30.593065039568383	24.959817360947596
50	19.023792217365042	25.713817709026504	28.091247862897863	27.17114221071059
51	17.122667103471574	26.037326344454797	26.985585438016358	29.854421114057267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	204.5
2	346.0
3	540.5
4	735.0
5	609.5
6	484.0
7	511.5
8	539.0
9	600.5
10	662.0
11	670.5
12	679.0
13	717.0
14	755.0
15	784.5
16	814.0
17	780.0
18	746.0
19	864.5
20	983.0
21	1325.5
22	1668.0
23	1804.0
24	1940.0
25	2053.0
26	2813.0
27	3460.0
28	4283.0
29	5106.0
30	5796.0
31	6486.0
32	7437.5
33	8389.0
34	9497.0
35	10605.0
36	12122.0
37	13639.0
38	14967.0
39	16295.0
40	17947.5
41	19600.0
42	22393.5
43	25187.0
44	29603.5
45	34020.0
46	37743.0
47	41466.0
48	40533.0
49	39600.0
50	34821.5
51	30043.0
52	26828.0
53	23613.0
54	21374.0
55	19135.0
56	18961.5
57	18788.0
58	18367.0
59	17946.0
60	16355.0
61	14764.0
62	13119.5
63	11475.0
64	9419.5
65	7364.0
66	5828.5
67	4293.0
68	3539.0
69	2785.0
70	2142.5
71	1500.0
72	1306.0
73	1112.0
74	889.0
75	523.0
76	380.0
77	290.0
78	200.0
79	167.0
80	134.0
81	99.5
82	65.0
83	37.5
84	10.0
85	8.5
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.17626746749447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20219925668641	27.21384538775399
2	10.460542507051692	7.777678521944977
3	3.7504034980896765	4.18278010668026
4	1.8687497981520678	2.778925693037103
5	1.165660492055658	2.1667453114476176
6	0.9321679021393044	2.0792713952726394
7	0.7851466117419617	2.043217430752134
8	0.6876533413703618	2.045150763496068
9	0.6526073060319141	2.1835353384255107
>10	6.358386104618097	41.51209475500489
>50	0.09888067271897114	2.4665538448519486
>100	0.03481713828132786	2.0792779682882925
>500	0.0	0.0
>1k	0.002785371062506229	1.4709234830445814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1651	0.4225575609905916	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1400	0.35831652658196744	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1355	0.34679920965611855	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1276	0.32657991994185037	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3621556322239171	0.0
2	0.0	0.0	0.0	1.6426253340021908	0.0
3	0.0	0.0	0.0	2.1207219566129876	0.0
4	0.0	0.0	0.0	2.881888635223538	0.0
5	0.0	0.0	0.0	4.07329108610858	0.0
6	0.0	0.0	0.0	4.701112828755413	0.0
7	0.0	0.0	0.0	5.300269249275689	0.0
8	0.0	0.0	0.0	6.295365431669039	0.0
9	0.0	0.0	0.0	6.739677924630678	0.0
10	0.0	0.0	0.0	7.615249951371329	0.0
11	0.0	0.0	0.0	9.25019707408962	0.0
12	0.0	0.0	0.0	10.752311141596454	0.0
13	0.0	0.0	0.0	11.273149807020957	0.0
14	0.0	0.0	0.0	11.45563529520163	0.0
15	0.0	0.0	0.0	11.759436521667912	0.0
16	0.0	0.0	0.0	12.461736913768569	0.0
17	0.0	0.0	0.0	13.479355849261356	0.0
18	0.0	0.0	0.0	14.621361807553312	0.0
19	0.0	0.0	0.0	15.350024058395356	0.0
20	0.0	0.0	0.0	16.142927343646026	0.0
21	0.0	0.0	0.0	17.222227909786135	0.0
22	0.0	0.0	0.0	18.27951760357907	0.0
23	0.0	0.0	0.0	19.28510734139375	0.0
24	0.0	0.0	0.0	20.04601807962817	0.0
25	0.0	0.0	0.0	20.751389756242386	0.0
26	0.0	0.0	0.0	21.387145650549247	0.0
27	0.0	0.0	0.0	21.978111979033365	0.0
28	0.0	0.0	0.0	22.655586154649413	0.0
29	0.0	0.0	0.0	23.34995239509004	0.0
30	0.0	0.0	0.0	24.175871988861473	0.0
31	0.0	0.0	0.0	24.948044103645614	0.0
32	0.0	0.0	0.0	25.637547476939773	0.0
33	0.0	0.0	0.0	26.32014046007842	0.0
34	0.0	0.0	0.0	26.99582305306156	0.0
35	0.0	0.0	0.0	27.712712046601624	0.0
36	0.0	0.0	0.0	28.41014957155581	0.0
37	0.0	0.0	0.0	29.096837600712536	0.0
38	0.0	0.0	0.0	29.810399369362912	0.0
39	0.0	0.0	0.0	30.540597262461738	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCT	25	3.886063E-5	45.0	6
CAAACGG	25	3.886063E-5	45.0	35
CACGGTC	20	7.027566E-4	45.0	45
GTCGTTT	20	7.027566E-4	45.0	9
GACGGGT	20	7.027566E-4	45.0	4
TTTATCG	20	7.027566E-4	45.0	1
CCAACGG	20	7.027566E-4	45.0	7
CGCAAGA	20	7.027566E-4	45.0	23
TCGACGT	20	7.027566E-4	45.0	26
ATCGCAA	20	7.027566E-4	45.0	21
TTACACG	135	0.0	43.333336	34
CGTTTGG	270	0.0	42.500004	2
CGAGATA	175	0.0	42.42857	19
GCGATTC	80	0.0	42.1875	9
TCCCGCT	60	3.6379788E-12	41.250004	14
GCCGATT	55	6.002665E-11	40.909092	9
TGGGCGA	485	0.0	40.36082	6
ACACGCG	140	0.0	40.17857	36
CACGCGG	140	0.0	40.17857	37
GGCCGAT	90	0.0	40.0	8
>>END_MODULE