FastQCFastQC Report
Sat 14 Jan 2017
SRR2934037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131862
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC11530.8743989928865026RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT8800.667364365776342RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC6860.5202408578665575Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC6860.5202408578665575Illumina PCR Primer Index 7 (95% over 22bp)
GCAGCTGGTACAATAATCAAGGAATTGTTTAAAACCAACTTATAAGTGAAT2350.17821662040618222No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC2110.16001577406682743No Hit
TTTTCTGGGACAAATATTTTGAAAAATATGAATAAACATGAACTTTTAAAA1930.14636513931231135No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG1850.14029819053252643RNA PCR Primer, Index 16 (95% over 24bp)
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1640.124372449985591No Hit
TGGCCTGTCCTATTTTGTTTTGAGGGAGTCATTAACCTTACTGTCACTAAA1600.12133897559569855No Hit
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG1570.11906386980327918Illumina PCR Primer Index 7 (95% over 21bp)
TGGCTTGGGGAAAAATAGCAAAGTATTTTGAGTACTTCAAAACATTTTCAT1510.1145136582184405No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1450.10996344663360179No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC1420.10768834084118244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCA453.783498E-1045.00000413
CGGTTAA600.045.00000435
TCGGGCG302.1508586E-645.0000045
GTTAGGG302.1508586E-645.0000043
TACGGTT600.045.00000433
ATATGAA453.783498E-1045.00000426
CCTAATA302.1508586E-645.00000416
ACCCTAA302.1508586E-645.00000414
AATATGA453.783498E-1045.00000425
TCTAATT302.1508586E-645.00000411
CGGGCCC302.1508586E-645.0000046
GCCTCCG453.783498E-1045.00000444
CTACATC302.1508586E-645.00000418
TGGTGTC302.1508586E-645.0000046
TTTCTCG302.1508586E-645.0000041
TATAAGT453.783498E-1045.00000441
TGTTTAA502.1827873E-1145.026
GAGGATA253.8719823E-545.09
ACCGGCG207.0105644E-445.020
GATGTTG253.8719823E-545.034
Produced by FastQC (version 0.11.5)