##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934037.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131862
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.973100665847628	31.0	30.0	31.0	26.0	34.0
2	30.317369674356524	31.0	30.0	33.0	27.0	34.0
3	30.328783121748494	31.0	30.0	34.0	26.0	34.0
4	34.018542112208216	35.0	33.0	37.0	30.0	37.0
5	34.41778526034794	35.0	35.0	37.0	32.0	37.0
6	33.89727897347227	35.0	35.0	37.0	30.0	37.0
7	34.42693876931944	35.0	35.0	37.0	32.0	37.0
8	34.52997072697214	35.0	35.0	37.0	32.0	37.0
9	35.76928910527673	37.0	35.0	39.0	30.0	39.0
10	35.31435136733858	37.0	34.0	39.0	30.0	39.0
11	35.47257739151537	37.0	35.0	39.0	30.0	39.0
12	35.22722998286087	37.0	34.0	39.0	30.0	39.0
13	34.88607028560161	37.0	33.0	39.0	27.0	39.0
14	35.449121050795526	38.0	33.0	40.0	27.0	41.0
15	35.96406849585173	38.0	34.0	40.0	30.0	41.0
16	36.17324172240676	38.0	34.0	40.0	30.0	41.0
17	36.08571081888641	38.0	34.0	40.0	30.0	41.0
18	36.20281809770821	38.0	35.0	40.0	30.0	41.0
19	36.15619359633556	38.0	34.0	40.0	30.0	41.0
20	36.25258982876037	38.0	34.0	40.0	30.0	41.0
21	35.89940240554519	38.0	34.0	40.0	29.0	41.0
22	36.07054344693695	38.0	34.0	40.0	30.0	41.0
23	36.22228541960534	38.0	34.0	40.0	30.0	41.0
24	36.107096813335154	38.0	34.0	40.0	30.0	41.0
25	35.62302255388209	37.0	34.0	40.0	29.0	41.0
26	35.66643915608743	38.0	34.0	40.0	29.0	41.0
27	35.698722907281855	38.0	34.0	40.0	29.0	41.0
28	35.62699640533285	38.0	34.0	40.0	29.0	41.0
29	35.78601871653699	38.0	34.0	40.0	30.0	41.0
30	35.40343692648375	37.0	34.0	40.0	29.0	41.0
31	35.43600885774522	37.0	34.0	40.0	29.0	41.0
32	35.49096024631812	37.0	34.0	40.0	29.0	41.0
33	35.5191184723423	37.0	34.0	40.0	29.0	41.0
34	35.541186998528765	37.0	34.0	40.0	29.0	41.0
35	35.65821844049081	38.0	34.0	40.0	30.0	41.0
36	35.38412886199208	37.0	34.0	40.0	29.0	41.0
37	35.37209355235019	37.0	34.0	40.0	29.0	41.0
38	35.263912271920645	37.0	34.0	40.0	29.0	41.0
39	35.272208824377	37.0	34.0	40.0	29.0	41.0
40	35.16872942925179	37.0	34.0	40.0	28.0	41.0
41	35.15611017578984	37.0	34.0	40.0	28.0	41.0
42	35.38799654183919	37.0	34.0	40.0	29.0	41.0
43	35.35569003958684	37.0	34.0	40.0	29.0	41.0
44	35.19206443099604	37.0	34.0	40.0	28.0	41.0
45	35.289401040481714	37.0	34.0	40.0	29.0	41.0
46	35.20143028317484	37.0	34.0	40.0	29.0	41.0
47	35.17813319986046	37.0	34.0	40.0	28.0	41.0
48	35.24757701233107	37.0	34.0	40.0	29.0	41.0
49	35.296180855743124	37.0	34.0	40.0	29.0	41.0
50	35.107263654426596	37.0	34.0	40.0	28.0	41.0
51	33.99162002699792	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	2.0
11	5.0
12	6.0
13	3.0
14	3.0
15	9.0
16	14.0
17	19.0
18	41.0
19	77.0
20	145.0
21	238.0
22	396.0
23	542.0
24	760.0
25	1100.0
26	1564.0
27	1942.0
28	2556.0
29	3299.0
30	4417.0
31	5561.0
32	7333.0
33	9620.0
34	14335.0
35	13306.0
36	14848.0
37	18762.0
38	21751.0
39	9205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.300586977294444	4.554003427826061	54.07850631721042	19.066903277669077
2	19.429403467261228	4.153584808360256	53.42175911179869	22.99525261257982
3	20.275742822041227	4.145242753788051	54.03300420136203	21.546010222808697
4	20.460026391227192	5.087136551849661	47.3623940179885	27.09044303893464
5	17.760234184222902	5.984286602660357	50.12361408138811	26.131865131728627
6	20.367505422335473	6.685777555322988	52.37066023570096	20.576056786640578
7	80.98845762994647	2.7794209097389695	10.675554746629052	5.55656671368552
8	81.50414827622818	3.28828623864343	8.140328525276425	7.067236959851966
9	74.76300981328964	6.401389331270571	11.932171512642006	6.903429342797773
10	41.34625593423428	27.610683896801202	16.99807374376242	14.044986425202104
11	32.8548027483278	25.090625047398035	24.002366110024116	18.05220609425005
12	30.880769289105274	21.7348440035795	29.00608211615173	18.378304591163488
13	22.53340613671869	31.6755395792571	28.171118290333837	17.619935993690373
14	16.351943698715324	33.29086469187484	29.363273725561573	20.993917883848265
15	14.456780573630008	24.69475663951707	41.606376363167556	19.242086423685368
16	15.677754015561723	23.09763237323869	37.429282128285635	23.795331482913955
17	15.902231120413765	23.585263381413903	29.230559221003777	31.281946277168554
18	19.609136824862354	21.38523608014439	34.70825560055209	24.29737149444116
19	22.446952116606756	25.31813562663997	28.714868574722058	23.520043682031215
20	25.52668699094508	25.701870136961368	28.19917792844034	20.572264943653213
21	22.781392668092398	26.856865510912925	28.696667728382703	21.665074092611974
22	19.652363834918322	26.745385327084374	26.994130227055557	26.608120610941743
23	18.80374937434591	28.06722179248002	26.445829731082494	26.68319910209158
24	21.03638652530676	23.810498854863418	31.727867012482747	23.425247607347075
25	17.90659932353521	25.940756245165403	30.453807768727913	25.698836662571477
26	17.50769744126435	31.878023994782424	27.113952465456308	23.500326098496913
27	20.293185299783108	29.517980919446085	28.976505740850282	21.21232803992052
28	16.733403103244303	27.245908601416634	34.683229436835475	21.337458858503584
29	15.895405803036509	25.28552577694863	32.343662313630915	26.475406106383947
30	19.56135960322155	26.611912453929108	28.64282355796211	25.183904384887228
31	22.261151810225844	30.789765057408502	23.41008023539761	23.539002896968043
32	21.676449621574072	29.012907433528994	23.631523865859762	25.67911907903718
33	21.877417299904444	26.76889475360604	24.992037129726533	26.361650816762978
34	16.94726304773172	27.753257193126146	27.114710834053785	28.18476892508835
35	18.247865192398113	26.36316755395793	25.955165248517385	29.43380200512657
36	23.600430753363362	24.549149868802232	26.799229497504967	25.05118988032943
37	20.149095266263213	28.09679816778147	28.2848735799548	23.469232986000517
38	16.573387329177475	29.151688886866573	25.287042514143575	28.987881269812384
39	18.578513900896393	26.3639259225554	27.659219487039483	27.39834068950873
40	21.389786291729234	25.855060593650936	26.277471902443462	26.477681212176368
41	16.653016031912152	25.30069314889809	25.59797363910755	32.448317180082206
42	20.837693952768806	24.40202636089245	25.40079780376454	29.359481882574208
43	21.945670473677026	23.24172240675858	27.034323762721634	27.77828335684276
44	18.172028332650804	25.698836662571477	29.00911559054163	27.120019414236097
45	18.48826803779709	27.5515311461983	24.62195325415965	29.33824756184496
46	20.795225311310308	26.994888595653034	27.492378395595395	24.717507697441267
47	16.795589328237096	25.911179869863947	30.507651939148506	26.78557886275045
48	18.67558508137295	21.687066781938693	30.774597685459042	28.862750451229314
49	18.372237642383705	21.61047155359391	32.55373041513097	27.463560388891416
50	19.684215316012192	23.61938996830019	28.667091353081254	28.029303362606363
51	17.591876355583867	24.888140631872716	25.240782029697716	32.2792009828457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	53.5
2	77.0
3	133.0
4	189.0
5	148.0
6	107.0
7	133.0
8	159.0
9	220.5
10	282.0
11	290.5
12	299.0
13	316.5
14	334.0
15	315.5
16	297.0
17	268.5
18	240.0
19	362.5
20	485.0
21	502.0
22	519.0
23	489.5
24	460.0
25	624.5
26	993.0
27	1197.0
28	1187.5
29	1178.0
30	1761.5
31	2345.0
32	2581.0
33	2817.0
34	2989.5
35	3162.0
36	3165.5
37	3169.0
38	4119.5
39	5070.0
40	6243.5
41	7417.0
42	8352.0
43	9287.0
44	9664.0
45	10041.0
46	10480.0
47	10919.0
48	12192.5
49	13466.0
50	14402.5
51	15339.0
52	14493.5
53	13648.0
54	10922.0
55	8196.0
56	6820.5
57	5445.0
58	4788.5
59	4132.0
60	3821.5
61	3511.0
62	3027.0
63	2543.0
64	2306.0
65	2069.0
66	1546.0
67	1023.0
68	809.5
69	596.0
70	516.0
71	436.0
72	381.5
73	327.0
74	249.5
75	114.5
76	57.0
77	40.0
78	23.0
79	13.5
80	4.0
81	3.0
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	131862.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.293170132411156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.25252525252525	31.07415328146092
2	10.532598714416896	8.698487813016637
3	3.7043158861340677	4.588888383309824
4	1.7741046831955924	2.93033626063612
5	1.1294765840220387	2.331983437229831
6	0.8099173553719008	2.0066433089138647
7	0.7144168962350781	2.0650376909192945
8	0.5344352617079889	1.7654820949174137
9	0.5215794306703397	1.9383901351412842
>10	4.894398530762167	35.49392546753424
>50	0.08999081726354453	2.4290546177063903
>100	0.03489439853076217	2.095372434818219
>500	0.005509641873278237	1.707846081509457
>1k	0.0018365472910927456	0.8743989928865026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1153	0.8743989928865026	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	880	0.667364365776342	RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	686	0.5202408578665575	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	686	0.5202408578665575	Illumina PCR Primer Index 7 (95% over 22bp)
GCAGCTGGTACAATAATCAAGGAATTGTTTAAAACCAACTTATAAGTGAAT	235	0.17821662040618222	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	211	0.16001577406682743	No Hit
TTTTCTGGGACAAATATTTTGAAAAATATGAATAAACATGAACTTTTAAAA	193	0.14636513931231135	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	185	0.14029819053252643	RNA PCR Primer, Index 16 (95% over 24bp)
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	164	0.124372449985591	No Hit
TGGCCTGTCCTATTTTGTTTTGAGGGAGTCATTAACCTTACTGTCACTAAA	160	0.12133897559569855	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	157	0.11906386980327918	Illumina PCR Primer Index 7 (95% over 21bp)
TGGCTTGGGGAAAAATAGCAAAGTATTTTGAGTACTTCAAAACATTTTCAT	151	0.1145136582184405	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	145	0.10996344663360179	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	142	0.10768834084118244	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7447179627185998	0.0
2	0.0	0.0	0.0	2.9439868953906356	0.0
3	0.0	0.0	0.0	3.698563649876386	0.0
4	0.0	0.0	0.0	4.669275454641975	0.0
5	0.0	0.0	0.0	6.164019960261485	0.0
6	0.0	0.0	0.0	7.05586143088987	0.0
7	0.0	0.0	0.0	7.899925679877447	0.0
8	0.0	0.0	0.0	9.349926438246046	0.0
9	0.0	0.0	0.0	10.023357752802172	0.0
10	0.0	0.0	0.0	11.229163822784425	0.0
11	0.0	0.0	0.0	13.432982967041301	0.0
12	0.0	0.0	0.0	15.463894071074305	0.0
13	0.0	0.0	0.0	16.14945928319	0.0
14	0.0	0.0	0.0	16.427022189865163	0.0
15	0.0	0.0	0.0	16.79938117122446	0.0
16	0.0	0.0	0.0	17.717765542764404	0.0
17	0.0	0.0	0.0	19.18672551606983	0.0
18	0.0	0.0	0.0	21.112223385054072	0.0
19	0.0	0.0	0.0	21.987380746538047	0.0
20	0.0	0.0	0.0	23.013453458919173	0.0
21	0.0	0.0	0.0	24.150247986531372	0.0
22	0.0	0.0	0.0	25.250640821464867	0.0
23	0.0	0.0	0.0	26.28353885122325	0.0
24	0.0	0.0	0.0	27.091201407532118	0.0
25	0.0	0.0	0.0	27.81468504952147	0.0
26	0.0	0.0	0.0	28.456264882983724	0.0
27	0.0	0.0	0.0	29.072060184131896	0.0
28	0.0	0.0	0.0	29.726532283751194	0.0
29	0.0	0.0	0.0	30.441673871168344	0.0
30	0.0	0.0	0.0	31.34413250216135	0.0
31	0.0	0.0	0.0	32.15558690145758	0.0
32	0.0	0.0	0.0	32.851769273937904	0.0
33	0.0	0.0	0.0	33.51306669093446	0.0
34	0.0	0.0	0.0	34.19787353445268	0.0
35	0.0	0.0	0.0	34.940316391378865	0.0
36	0.0	0.0	0.0	35.641807344041496	0.0
37	0.0	0.0	0.0	36.319788870182464	0.0
38	0.0	0.0	0.0	37.00307897650574	0.0
39	0.0	0.0	0.0	37.790265580682835	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATCA	45	3.783498E-10	45.000004	13
CGGTTAA	60	0.0	45.000004	35
TCGGGCG	30	2.1508586E-6	45.000004	5
GTTAGGG	30	2.1508586E-6	45.000004	3
TACGGTT	60	0.0	45.000004	33
ATATGAA	45	3.783498E-10	45.000004	26
CCTAATA	30	2.1508586E-6	45.000004	16
ACCCTAA	30	2.1508586E-6	45.000004	14
AATATGA	45	3.783498E-10	45.000004	25
TCTAATT	30	2.1508586E-6	45.000004	11
CGGGCCC	30	2.1508586E-6	45.000004	6
GCCTCCG	45	3.783498E-10	45.000004	44
CTACATC	30	2.1508586E-6	45.000004	18
TGGTGTC	30	2.1508586E-6	45.000004	6
TTTCTCG	30	2.1508586E-6	45.000004	1
TATAAGT	45	3.783498E-10	45.000004	41
TGTTTAA	50	2.1827873E-11	45.0	26
GAGGATA	25	3.8719823E-5	45.0	9
ACCGGCG	20	7.0105644E-4	45.0	20
GATGTTG	25	3.8719823E-5	45.0	34
>>END_MODULE