##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934036.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415532
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05968012090525	31.0	30.0	31.0	26.0	34.0
2	30.218945833293223	31.0	30.0	33.0	26.0	34.0
3	30.240924886651328	31.0	30.0	34.0	25.0	34.0
4	34.02961264114436	35.0	33.0	37.0	28.0	37.0
5	34.487974452027764	35.0	35.0	37.0	32.0	37.0
6	33.91958020080283	35.0	35.0	37.0	30.0	37.0
7	34.41456013014641	35.0	35.0	37.0	32.0	37.0
8	34.39397206472666	35.0	35.0	37.0	31.0	37.0
9	35.887534534043105	37.0	35.0	39.0	31.0	39.0
10	35.44089263883407	37.0	34.0	39.0	30.0	39.0
11	35.55327868852459	37.0	35.0	39.0	30.0	39.0
12	35.46816129684356	37.0	35.0	39.0	30.0	39.0
13	35.36168814916781	37.0	35.0	39.0	30.0	39.0
14	35.90855337254411	38.0	34.0	40.0	27.0	41.0
15	36.235358528344385	38.0	35.0	40.0	30.0	41.0
16	36.48027348074276	38.0	35.0	40.0	31.0	41.0
17	36.27724940558128	38.0	35.0	40.0	30.0	41.0
18	36.436281682277176	38.0	35.0	40.0	31.0	41.0
19	36.40283299481147	38.0	35.0	40.0	30.0	41.0
20	36.3344267108189	38.0	34.0	40.0	30.0	41.0
21	36.06338621333616	38.0	34.0	40.0	30.0	41.0
22	36.283997381669764	38.0	34.0	40.0	30.0	41.0
23	36.35252399333866	38.0	35.0	40.0	30.0	41.0
24	36.28321043866658	38.0	35.0	40.0	30.0	41.0
25	35.87547048121444	38.0	34.0	40.0	29.0	41.0
26	35.9792266299587	38.0	34.0	40.0	30.0	41.0
27	35.95605633260495	38.0	34.0	40.0	30.0	41.0
28	35.8800236804867	38.0	34.0	40.0	30.0	41.0
29	36.00233195036724	38.0	34.0	40.0	30.0	41.0
30	35.55832523126979	38.0	34.0	40.0	29.0	41.0
31	35.637443085009096	38.0	34.0	40.0	29.0	41.0
32	35.63453356179548	38.0	34.0	40.0	29.0	41.0
33	35.64231154279334	38.0	34.0	40.0	29.0	41.0
34	35.57626127470327	38.0	34.0	40.0	29.0	41.0
35	35.603662293156724	38.0	34.0	40.0	29.0	41.0
36	35.394903400941445	38.0	34.0	40.0	28.0	41.0
37	35.46998787097023	38.0	34.0	40.0	28.0	41.0
38	35.38105849850312	38.0	34.0	40.0	28.0	41.0
39	35.25235360934898	37.0	34.0	40.0	27.0	41.0
40	35.14108901360184	37.0	34.0	40.0	27.0	41.0
41	34.914377232078394	37.0	33.0	40.0	26.0	41.0
42	35.227453000009625	37.0	34.0	40.0	28.0	41.0
43	35.25678648094491	37.0	34.0	40.0	28.0	41.0
44	35.269757804453086	37.0	34.0	40.0	28.0	41.0
45	35.32604468488588	37.0	34.0	40.0	28.0	41.0
46	35.20997179519267	37.0	34.0	40.0	28.0	41.0
47	35.08891733969947	37.0	34.0	40.0	28.0	41.0
48	35.079500495750025	37.0	34.0	40.0	28.0	41.0
49	35.09145384711647	37.0	34.0	40.0	28.0	41.0
50	34.89390468122792	37.0	34.0	40.0	27.0	41.0
51	33.77050864915338	35.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	7.0
12	9.0
13	10.0
14	17.0
15	22.0
16	51.0
17	90.0
18	159.0
19	318.0
20	635.0
21	1169.0
22	1660.0
23	2250.0
24	3052.0
25	3917.0
26	4843.0
27	5950.0
28	7599.0
29	9856.0
30	12723.0
31	16538.0
32	21531.0
33	29014.0
34	39646.0
35	39752.0
36	47432.0
37	60999.0
38	71880.0
39	34395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.128914259310953	4.778693337697217	50.46951859303255	18.62287380995928
2	18.83874166129203	7.1614219843477755	53.129241550590564	20.870594803769627
3	22.475766005987506	7.3022053656517425	49.300414889827984	20.92161373853277
4	19.986427038110183	5.215001492063187	48.36498753405273	26.433583935773903
5	20.665315787953755	5.936004928621622	48.59361011907627	24.805069164348353
6	22.227169026693492	9.081370387840167	50.94409094847088	17.747369636995465
7	81.74532887960494	2.8443056130454454	11.296602909041903	4.113762598307711
8	83.57430955979322	5.512932818651752	6.260648999355044	4.652108622199975
9	77.3350788868246	5.735298364506224	8.912670985628063	8.016951763041114
10	43.56415390391113	24.621689785624213	16.993637072475767	14.820519237988892
11	34.213249521095854	24.105965364881644	23.38905306931837	18.291732044704137
12	28.86949741536151	21.72443999499437	28.870460036772137	20.535602552871982
13	27.26769538808082	23.227573327685956	30.096358403203606	19.40837288102962
14	20.430436163761154	27.155068683037648	30.957423255008038	21.45707189819316
15	18.332643454655717	24.26600117439812	35.80783188779685	21.593523483149312
16	22.869478162933298	23.81669763098871	30.935282962563655	22.378541243514338
17	23.160189828942176	24.62313371774015	28.73497107322661	23.481705380091064
18	24.0681824745146	22.733026577977146	30.29995283155088	22.898838115957375
19	25.48251398207599	24.575243302561535	27.51268253708499	22.429560178277484
20	27.087685184293868	27.100921228689966	28.205288642029974	17.606104944986186
21	25.687311687186547	27.212104001617206	28.3275415611794	18.773042750016845
22	23.345253795134912	23.0227756225754	30.85899521577159	22.772975366518104
23	22.116708219824226	26.01388100074122	29.250695493969175	22.61871528546538
24	22.584542225388176	25.50296968705178	29.310137366075296	22.602350721484747
25	22.386964180857312	27.256384586505973	27.117285792670597	23.239365439966118
26	21.22941193458025	26.281008442189773	28.409364381082565	24.080215242147414
27	21.952821924665248	26.677127152662127	29.036993540810336	22.333057381862286
28	19.951532011975008	26.79071647911593	30.323296400758547	22.934455108150516
29	21.545392412618042	25.61655901350558	30.08456629092344	22.753482282952938
30	23.491572249549975	25.672150399969198	29.21146867148619	21.62480867899464
31	25.113348671101143	24.823358971150235	27.552390670273287	22.510901687475332
32	26.848233108400798	26.268735019204296	26.328898857368387	20.554133015026522
33	25.643512413003087	25.891868736944446	25.74314372900282	22.721475121049643
34	22.40092219131138	25.130675856492402	30.0886574319186	22.37974452027762
35	22.85768605065314	26.09353792247047	27.765130002021504	23.283646024854885
36	23.90501814541359	27.464792121906374	27.200793200042355	21.42939653263768
37	22.70703579989026	26.819354466082036	27.665017375316463	22.808592358711245
38	25.225494065439	26.580383700894277	25.656748457399193	22.537373776267533
39	24.375018049151446	25.489011676597713	27.696543226514443	22.439427047736395
40	25.812211815215193	23.35054821289335	27.856579036030922	22.980660935860538
41	20.790697226687715	25.195171491004302	28.27098755330516	25.74314372900282
42	23.023256933280713	24.64888384047438	28.804279814791638	23.52357941145327
43	22.919053165580507	25.413927206568925	28.247403328744834	23.419616299105726
44	23.191715680140156	24.089600800901014	28.76794085654053	23.950742662418296
45	23.080292251860264	23.676876871095367	26.59145384711647	26.651377029927897
46	23.500717152950916	25.00529441775844	28.14512480386589	23.348863625424755
47	20.429714197703184	25.756620428751575	31.431995610446368	22.38166976309887
48	21.850784055138956	26.34863259628621	29.02857060346736	22.772012745107475
49	24.305949962939074	23.597941915424084	29.858590914779125	22.237517206857717
50	22.450497193958586	25.35568861122609	27.98340440688082	24.210409787934502
51	21.31123475448341	25.15690728993194	26.833793787241415	26.698064168343233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	230.0
2	377.0
3	610.0
4	843.0
5	666.0
6	489.0
7	504.0
8	519.0
9	574.5
10	630.0
11	633.0
12	636.0
13	663.0
14	690.0
15	728.5
16	767.0
17	827.0
18	887.0
19	1056.0
20	1225.0
21	1382.0
22	1539.0
23	1823.0
24	2107.0
25	2360.5
26	3284.0
27	3954.0
28	4473.5
29	4993.0
30	6095.5
31	7198.0
32	8437.0
33	9676.0
34	11063.5
35	12451.0
36	13331.0
37	14211.0
38	15403.5
39	16596.0
40	18183.0
41	19770.0
42	21000.0
43	22230.0
44	23604.0
45	24978.0
46	26421.5
47	27865.0
48	33115.5
49	38366.0
50	38217.0
51	38068.0
52	37207.5
53	36347.0
54	33374.5
55	30402.0
56	27416.0
57	24430.0
58	22567.0
59	20704.0
60	17981.0
61	15258.0
62	13422.5
63	11587.0
64	9839.5
65	8092.0
66	6898.0
67	5704.0
68	4662.5
69	3621.0
70	3087.0
71	2553.0
72	2028.5
73	1504.0
74	1178.0
75	650.0
76	448.0
77	303.5
78	159.0
79	113.0
80	67.0
81	50.5
82	34.0
83	19.0
84	4.0
85	3.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.614013298081964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78819756048347	24.803174540395904
2	9.054624648076555	6.087245466591721
3	3.1907181446725987	3.2175852643636844
4	1.6333383004233573	2.196122214027893
5	1.167970280840877	1.9630084275974882
6	0.9613503297233958	1.9388905660462628
7	0.7928234121807591	1.8654983704052326
8	0.7779749944583821	2.092068944743944
9	0.7351529970011634	2.2240298355589725
>10	7.773405915395715	46.905645696983306
>50	0.08320344724145963	1.9035276128587741
>100	0.03472839537034837	2.099790137910139
>500	0.0014470164737645152	0.2794369780471102
>1k	0.005064557658175804	2.42397594446958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	2147	0.5166870421531916	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	1793	0.43149504731284233	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1519	0.3655554806850014	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	1407	0.33860208118748975	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1038	0.24980025605729522	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	1036	0.2493189453519825	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1027	0.24715304717807535	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	592	0.14246796877256143	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	557	0.13404503142958904	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	464	0.11166408363254816	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	453	0.10901687475332825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2743471020282433	0.0
2	0.0	0.0	0.0	1.1043674133400074	0.0
3	0.0	0.0	0.0	1.3714948547885601	0.0
4	0.0	0.0	0.0	1.8439013120529826	0.0
5	0.0	0.0	0.0	2.8293849811807514	0.0
6	0.0	0.0	0.0	3.205288642029976	0.0
7	0.0	0.0	0.0	3.637265000048131	0.0
8	0.0	0.0	0.0	4.302436394790293	0.0
9	0.0	0.0	0.0	4.540685193920083	0.0
10	0.0	0.0	0.0	5.520152479231443	0.0
11	0.0	0.0	0.0	6.511893187528277	0.0
12	0.0	0.0	0.0	8.068452008509574	0.0
13	0.0	0.0	0.0	8.42317799832504	0.0
14	0.0	0.0	0.0	8.564202034981662	0.0
15	0.0	0.0	0.0	8.875128750613671	0.0
16	0.0	0.0	0.0	9.324191638670428	0.0
17	0.0	0.0	0.0	9.932087059480377	0.0
18	0.0	0.0	0.0	10.623971198367395	0.0
19	0.0	0.0	0.0	11.384923423466784	0.0
20	0.0	0.0	0.0	11.928082554412175	0.0
21	0.0	0.0	0.0	12.621891936120443	0.0
22	0.0	0.0	0.0	13.339044887036378	0.0
23	0.0	0.0	0.0	14.041999172145587	0.0
24	0.0	0.0	0.0	14.6087425276513	0.0
25	0.0	0.0	0.0	15.106899107649951	0.0
26	0.0	0.0	0.0	15.563903622344368	0.0
27	0.0	0.0	0.0	16.056284473879266	0.0
28	0.0	0.0	0.0	16.543852218361042	0.0
29	0.0	0.0	0.0	17.087492660011744	0.0
30	0.0	0.0	0.0	17.734855558657337	0.0
31	0.0	0.0	0.0	18.275608136076162	0.0
32	0.0	0.0	0.0	18.765823089437156	0.0
33	0.0	0.0	0.0	19.263498358730494	0.0
34	0.0	0.0	0.0	19.812192562786983	0.0
35	0.0	0.0	0.0	20.401798176795047	0.0
36	0.0	0.0	0.0	20.949289104088255	0.0
37	0.0	0.0	0.0	21.525418018347565	0.0
38	0.0	0.0	0.0	22.13957047832658	0.0
39	0.0	0.0	0.0	23.077163732275732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTGC	30	2.1621436E-6	45.000004	1
AGGCACG	30	2.1621436E-6	45.000004	10
TCGGACC	20	7.0280815E-4	45.0	6
CGCTATA	20	7.0280815E-4	45.0	22
TTGCCCG	25	3.886492E-5	45.0	1
CGATAAC	25	3.886492E-5	45.0	16
TGCGTTG	45	3.8380676E-10	45.0	1
GTCGGCG	35	1.2094279E-7	45.0	3
TGGGCGA	400	0.0	41.625	6
CCGATCG	65	0.0	41.538464	40
ATCCGGC	60	3.6379788E-12	41.250004	6
GCGATTC	60	3.6379788E-12	41.250004	9
CGGATGA	50	1.0786607E-9	40.5	44
TTTCGCG	140	0.0	40.17857	1
CGGGTCC	90	0.0	40.0	6
CGGGATA	90	0.0	40.0	6
TTACGGG	90	0.0	40.0	3
CTTTGCG	130	0.0	39.807693	1
TCGGGAT	165	0.0	39.545452	5
TCGGCGT	40	3.451114E-7	39.375	4
>>END_MODULE