##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329244
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.842560532614108	31.0	30.0	31.0	25.0	34.0
2	29.84653934468054	31.0	30.0	33.0	25.0	34.0
3	29.88286498766872	31.0	30.0	33.0	25.0	34.0
4	33.85674454204177	35.0	33.0	37.0	28.0	37.0
5	34.368231463595386	35.0	35.0	37.0	32.0	37.0
6	33.644233456038684	35.0	33.0	37.0	28.0	37.0
7	34.34946118987742	35.0	35.0	37.0	32.0	37.0
8	34.14295780636853	35.0	35.0	37.0	30.0	37.0
9	35.75134854393702	37.0	35.0	39.0	30.0	39.0
10	35.26378916548214	37.0	34.0	39.0	30.0	39.0
11	35.38636391247828	37.0	35.0	39.0	30.0	39.0
12	35.253805688182624	37.0	34.0	39.0	30.0	39.0
13	35.04986879031964	37.0	34.0	39.0	27.0	39.0
14	35.565285927761785	38.0	34.0	40.0	27.0	41.0
15	35.86981691389972	38.0	34.0	40.0	27.0	41.0
16	36.23682132400287	38.0	34.0	40.0	30.0	41.0
17	35.938777927615995	38.0	34.0	40.0	29.0	41.0
18	36.16235679313822	38.0	34.0	40.0	30.0	41.0
19	36.11592618240575	38.0	34.0	40.0	30.0	41.0
20	35.977357218354776	38.0	34.0	40.0	29.0	41.0
21	35.7714491380253	38.0	34.0	40.0	29.0	41.0
22	36.0434783929244	38.0	34.0	40.0	30.0	41.0
23	36.06100035232229	38.0	34.0	40.0	30.0	41.0
24	35.99023824276221	38.0	34.0	40.0	30.0	41.0
25	35.60031466025197	38.0	34.0	40.0	29.0	41.0
26	35.67829937675402	38.0	34.0	40.0	29.0	41.0
27	35.578692398342874	38.0	34.0	40.0	29.0	41.0
28	35.45912453985494	38.0	34.0	40.0	28.0	41.0
29	35.625344729137055	38.0	34.0	40.0	29.0	41.0
30	35.21321573058279	37.0	34.0	40.0	27.0	41.0
31	35.347086051682034	37.0	34.0	40.0	28.0	41.0
32	35.27087813293485	37.0	34.0	40.0	27.0	41.0
33	35.270623003000814	38.0	34.0	40.0	27.0	41.0
34	35.163902759048	38.0	34.0	40.0	27.0	41.0
35	35.143950990754576	37.0	34.0	40.0	27.0	41.0
36	34.99027165263452	37.0	33.0	40.0	26.0	41.0
37	34.935315449939864	37.0	33.0	40.0	26.0	41.0
38	34.83738503966663	37.0	33.0	40.0	26.0	41.0
39	34.83593322885155	37.0	33.0	40.0	26.0	41.0
40	34.68962836072943	37.0	33.0	40.0	26.0	41.0
41	34.49148655708228	37.0	33.0	40.0	24.0	41.0
42	34.70285563290447	37.0	33.0	40.0	26.0	41.0
43	34.74144403542661	37.0	33.0	40.0	26.0	41.0
44	34.77927615993002	37.0	33.0	40.0	26.0	41.0
45	34.794829974122536	37.0	33.0	40.0	27.0	41.0
46	34.71388089076794	37.0	33.0	40.0	26.0	41.0
47	34.52866870770614	36.0	33.0	40.0	26.0	41.0
48	34.528364981594194	36.0	33.0	40.0	26.0	41.0
49	34.55317636767868	36.0	33.0	40.0	26.0	41.0
50	34.34353245617232	36.0	33.0	39.0	26.0	41.0
51	33.24421705482864	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	5.0
13	3.0
14	11.0
15	17.0
16	31.0
17	77.0
18	172.0
19	378.0
20	789.0
21	1281.0
22	1857.0
23	2515.0
24	3108.0
25	3732.0
26	4471.0
27	5533.0
28	6915.0
29	8910.0
30	11247.0
31	14492.0
32	18364.0
33	23839.0
34	31918.0
35	31498.0
36	36304.0
37	45444.0
38	51841.0
39	24479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.382937881935586	4.418000024298089	49.55716732878959	15.641894764976735
2	19.214017567518315	9.805190071800853	50.89325849521935	20.08753386546148
3	21.736766653302716	9.960394115002854	48.568842560532616	19.73399667116181
4	19.88312619212499	4.832586167097958	49.43719551457278	25.847092126204274
5	22.63792202743254	5.673907497175347	47.456293812491644	24.23187666290046
6	21.49105222874221	11.324731809843156	49.36430124770686	17.819914713707767
7	80.80268736863846	2.4586628761647895	12.920205075870783	3.818444679325971
8	81.11066564614693	7.607731651905578	6.582048571879821	4.69955413006767
9	75.99925890828686	5.1761003997035635	8.19179696516869	10.632843726840884
10	44.226774064219846	24.60424487614049	15.865437183365527	15.303543876274132
11	36.06352735357364	23.75958255883175	23.099281991471372	17.07760809612324
12	29.16469244693905	21.31337245325655	27.628749498851917	21.893185600952485
13	26.668367532893537	24.395888763348765	30.728274471212842	18.20746923254486
14	20.50940943494794	29.024067257110232	30.752876286280085	19.713647021661746
15	17.64041258155046	23.407867721203726	37.19429966833109	21.757420028914726
16	21.85673846751953	23.027298902941283	30.90261325946714	24.21334937007204
17	22.316883527110594	25.062263852947964	28.676604585049386	23.944248034892055
18	24.753374397103666	21.61557993463814	30.3300895384578	23.30095612980039
19	24.727253951476715	24.23400274568405	26.48856167462429	24.55018162821494
20	26.96207068314077	26.211259734421887	28.657773566109025	18.168896016328315
21	24.30902309533355	28.66597417113144	28.494672643996548	18.530330089538456
22	22.710512568186513	22.119461554348753	32.504464773845534	22.6655611036192
23	23.060101323030942	26.33396508364617	28.65412885276573	21.951804740557154
24	22.352115756095785	25.891436138547704	28.943276111333844	22.81317199402267
25	21.19066710403227	29.55589167911944	26.37618301320601	22.877258203642285
26	21.15604232727096	26.24952922452649	28.51836328072797	24.076065167474578
27	22.77156151668671	27.34142459695545	28.112888921286338	21.774124965071497
28	18.19805372307468	29.038342384371468	29.792494320321705	22.971109572232145
29	21.36956178396569	25.221720061717146	29.621192793186818	23.787525361130346
30	22.83443282185856	27.91577067463644	28.425119364361993	20.824677139143006
31	25.51056359417332	26.07124199681695	25.298562768038295	23.119631640971438
32	27.396702749328767	27.66944879785205	24.443270036811604	20.490578416007583
33	25.746558783151706	26.25499629454143	24.547448093207468	23.45099682909939
34	23.57916924833862	25.558856045971982	27.74902503918067	23.11294966650873
35	21.870102416444947	29.0966577978642	25.869871584599874	23.163368201090982
36	25.859848622905808	29.56318110580603	23.96642004106377	20.61055023022439
37	21.239567008054816	29.837142058777076	26.541713744214018	22.38157718895409
38	22.193874451774366	29.794012950881417	23.4437074024128	24.56840519493142
39	22.774295051694185	26.393799127698607	25.275175857418812	25.556729963188396
40	25.63995091786031	24.969931114917813	25.785435725480195	23.60468224174169
41	21.928114103825735	26.83480944223737	25.21139337391114	26.025683080025757
42	22.20602349625202	26.646195526721822	27.548262079187474	23.599518897838685
43	22.514001773760494	27.275212304552248	26.438143140042037	23.772642781645224
44	22.390081520088444	26.087946932973722	27.51697828965752	24.004993257280315
45	21.877391843131537	25.46834566461348	25.23052811896344	27.42373437329154
46	24.0526782568551	26.35006256757906	26.13684683699627	23.460412338569572
47	19.62465527086295	28.02602325327113	30.44763154377908	21.90168993208684
48	21.427269745234536	28.542357643571332	27.31834141244791	22.712031198746217
49	22.518253939327675	25.47320528240454	29.437134769350394	22.5714060089174
50	21.95089356222133	26.761307723147574	26.29387323686992	24.993925477761174
51	21.02513637302426	26.426297821676325	24.830824555648697	27.71774124965071
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	123.5
2	211.0
3	376.0
4	541.0
5	426.0
6	311.0
7	336.5
8	362.0
9	382.5
10	403.0
11	428.0
12	453.0
13	546.5
14	640.0
15	656.0
16	672.0
17	635.0
18	598.0
19	714.0
20	830.0
21	891.5
22	953.0
23	1188.0
24	1423.0
25	1561.5
26	2444.5
27	3189.0
28	3568.0
29	3947.0
30	4789.5
31	5632.0
32	6401.0
33	7170.0
34	7777.5
35	8385.0
36	9044.0
37	9703.0
38	10675.5
39	11648.0
40	13620.5
41	15593.0
42	17033.0
43	18473.0
44	20186.0
45	21899.0
46	29017.0
47	36135.0
48	34603.0
49	33071.0
50	31513.0
51	29955.0
52	27156.0
53	24357.0
54	22336.0
55	20315.0
56	19355.5
57	18396.0
58	16942.5
59	15489.0
60	13660.0
61	11831.0
62	10175.5
63	8520.0
64	7214.5
65	5909.0
66	4856.0
67	3803.0
68	3190.5
69	2578.0
70	2093.5
71	1609.0
72	1371.0
73	1133.0
74	869.0
75	512.0
76	419.0
77	296.5
78	174.0
79	150.0
80	126.0
81	85.0
82	44.0
83	23.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329244.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.91763052328383
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2912828022696	22.7544882401378
2	10.421764978018807	6.65276087937807
3	3.7026987108178604	3.5454410817277147
4	1.9513498416965476	2.4912985307575517
5	1.2771305260748962	2.0381490130632822
6	0.9867292726292233	1.8896436210172889
7	0.8839588083085078	1.9749709448975261
8	0.7901744689346214	2.017639739870981
9	0.6824232925957364	1.9603201062198174
>10	7.854851513237178	45.808629052088094
>50	0.10874873804085573	2.313152185629772
>100	0.03990779377646082	2.4421206100763655
>500	0.0029930845332345616	0.5993051229839249
>1k	0.005986169066469123	3.5120808721518104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	3424	1.0399582072869968	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	2494	0.7574929231815918	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	1756	0.5333430525689155	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT	1336	0.4057780855535712	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1016	0.30858572973235654	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1003	0.3046372902771197	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	702	0.2132157305827897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA	667	0.2025853166648443	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC	513	0.15581149542588474	No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	489	0.14852206873929366	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	481	0.14609225984376328	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	447	0.13576557203775924	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	438	0.13303203703028757	No Hit
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	339	0.10296315194809928	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2557373862545711	0.0
2	0.0	0.0	0.0	1.1866579193546427	0.0
3	0.0	0.0	0.0	1.4706418340197545	0.0
4	0.0	0.0	0.0	2.081131319021759	0.0
5	0.0	0.0	0.0	3.413881498220165	0.0
6	0.0	0.0	0.0	3.8114589787513213	0.0
7	0.0	0.0	0.0	4.345105757432178	0.0
8	0.0	0.0	0.0	5.121125973442188	0.0
9	0.0	0.0	0.0	5.391138486958001	0.0
10	0.0	0.0	0.0	6.852364811507575	0.0
11	0.0	0.0	0.0	7.8993087193692215	0.0
12	0.0	0.0	0.0	10.157512361652756	0.0
13	0.0	0.0	0.0	10.56632770832574	0.0
14	0.0	0.0	0.0	10.738236687684514	0.0
15	0.0	0.0	0.0	11.174083658320273	0.0
16	0.0	0.0	0.0	11.711071424232484	0.0
17	0.0	0.0	0.0	12.398099889443696	0.0
18	0.0	0.0	0.0	13.14222886369987	0.0
19	0.0	0.0	0.0	14.152725638128562	0.0
20	0.0	0.0	0.0	14.758659231451446	0.0
21	0.0	0.0	0.0	15.486994447886675	0.0
22	0.0	0.0	0.0	16.255421511098152	0.0
23	0.0	0.0	0.0	16.94153879797354	0.0
24	0.0	0.0	0.0	17.489764430027577	0.0
25	0.0	0.0	0.0	18.000631750312838	0.0
26	0.0	0.0	0.0	18.465940153806905	0.0
27	0.0	0.0	0.0	18.973162760748867	0.0
28	0.0	0.0	0.0	19.480992819914714	0.0
29	0.0	0.0	0.0	20.056857528155412	0.0
30	0.0	0.0	0.0	20.7481381589338	0.0
31	0.0	0.0	0.0	21.30608302656996	0.0
32	0.0	0.0	0.0	21.831832926340343	0.0
33	0.0	0.0	0.0	22.356975373886844	0.0
34	0.0	0.0	0.0	22.93496616491113	0.0
35	0.0	0.0	0.0	23.582813961681914	0.0
36	0.0	0.0	0.0	24.141366281541956	0.0
37	0.0	0.0	0.0	24.71874962034236	0.0
38	0.0	0.0	0.0	25.38694706661321	0.0
39	0.0	0.0	0.0	26.494939922975057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.025944E-4	45.000004	6
CGGGTAC	20	7.025944E-4	45.000004	6
ACTTCGG	20	7.025944E-4	45.000004	44
TTATCCG	20	7.025944E-4	45.000004	1
ACGGCAG	20	7.025944E-4	45.000004	28
ACACTGC	20	7.025944E-4	45.000004	35
ACCCCGA	20	7.025944E-4	45.000004	37
GGTTAAC	20	7.025944E-4	45.000004	8
CCCGAAC	20	7.025944E-4	45.000004	40
CCGATCG	95	0.0	45.0	40
AGGAACG	25	3.8847225E-5	45.0	43
CCTCGTC	50	2.1827873E-11	45.0	36
CTACGGG	25	3.8847225E-5	45.0	3
CGATCGA	100	0.0	45.0	41
ACACGTG	30	2.1607666E-6	44.999996	42
AAACACG	30	2.1607666E-6	44.999996	40
GCATATC	30	2.1607666E-6	44.999996	28
TTTCGCG	130	0.0	43.26923	1
TGTTTCG	70	0.0	41.785717	1
TCCTAAC	70	0.0	41.785717	26
>>END_MODULE