##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934033.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489971
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.119839745617597	31.0	30.0	31.0	26.0	34.0
2	30.359331470638057	31.0	30.0	33.0	27.0	34.0
3	30.431307158995125	31.0	30.0	34.0	26.0	34.0
4	34.15176408399681	35.0	33.0	37.0	30.0	37.0
5	34.55891675221595	35.0	35.0	37.0	32.0	37.0
6	34.07054090956404	35.0	35.0	37.0	30.0	37.0
7	34.5968638960265	35.0	35.0	37.0	32.0	37.0
8	34.74907698618898	35.0	35.0	37.0	32.0	37.0
9	36.061901622749104	38.0	35.0	39.0	31.0	39.0
10	35.374497674352156	37.0	34.0	39.0	30.0	39.0
11	35.49508440295446	37.0	35.0	39.0	30.0	39.0
12	35.63665400605342	37.0	35.0	39.0	30.0	39.0
13	35.69567586653088	37.0	35.0	39.0	30.0	39.0
14	36.375999804070034	38.0	35.0	40.0	30.0	41.0
15	36.60931769431252	38.0	35.0	40.0	31.0	41.0
16	36.76390847621594	38.0	35.0	40.0	31.0	41.0
17	36.504189023432	38.0	35.0	40.0	31.0	41.0
18	36.604086772482454	38.0	35.0	40.0	31.0	41.0
19	36.635696806545695	38.0	35.0	40.0	31.0	41.0
20	36.67767888303594	38.0	35.0	40.0	31.0	41.0
21	36.39653367240102	38.0	35.0	40.0	30.0	41.0
22	36.5695765667764	38.0	35.0	40.0	30.0	41.0
23	36.63342524353482	38.0	35.0	40.0	31.0	41.0
24	36.54227903284072	38.0	35.0	40.0	30.0	41.0
25	36.09300142253317	38.0	34.0	40.0	30.0	41.0
26	36.20307936592166	38.0	35.0	40.0	30.0	41.0
27	36.265327947980595	38.0	35.0	40.0	30.0	41.0
28	36.19075210573687	38.0	35.0	40.0	30.0	41.0
29	36.35585167285411	38.0	35.0	40.0	30.0	41.0
30	35.9090252280237	38.0	34.0	40.0	29.0	41.0
31	36.06121994975213	38.0	34.0	40.0	30.0	41.0
32	36.08729496235492	38.0	35.0	40.0	30.0	41.0
33	36.07806788565038	38.0	35.0	40.0	30.0	41.0
34	36.03858187525384	38.0	35.0	40.0	30.0	41.0
35	36.04431078574038	38.0	35.0	40.0	30.0	41.0
36	35.8867157444012	38.0	34.0	40.0	29.0	41.0
37	35.98838706780605	38.0	35.0	40.0	30.0	41.0
38	35.88525443342565	38.0	34.0	40.0	29.0	41.0
39	35.83419630957751	38.0	34.0	40.0	29.0	41.0
40	35.76446769298591	38.0	34.0	40.0	29.0	41.0
41	35.64263599274243	38.0	34.0	40.0	29.0	41.0
42	35.85697316779973	38.0	34.0	40.0	30.0	41.0
43	35.86333272785532	38.0	34.0	40.0	30.0	41.0
44	35.84833592192191	38.0	34.0	40.0	29.0	41.0
45	35.93975153631541	38.0	34.0	40.0	30.0	41.0
46	35.81295219512992	38.0	34.0	40.0	29.0	41.0
47	35.741145904553534	38.0	34.0	40.0	29.0	41.0
48	35.74077853587253	38.0	34.0	40.0	29.0	41.0
49	35.771117066112076	38.0	34.0	40.0	29.0	41.0
50	35.62215518877648	38.0	34.0	40.0	29.0	41.0
51	34.565164060730126	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	6.0
13	8.0
14	9.0
15	13.0
16	27.0
17	69.0
18	138.0
19	254.0
20	494.0
21	871.0
22	1269.0
23	1915.0
24	2557.0
25	3421.0
26	4715.0
27	6021.0
28	7899.0
29	10391.0
30	13881.0
31	17975.0
32	23461.0
33	30605.0
34	42115.0
35	46826.0
36	57949.0
37	75367.0
38	94132.0
39	47576.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.980398839931343	4.419445232472943	54.57976084298867	17.02039508460705
2	18.884382953276827	5.4131775145875975	56.30312814431875	19.39931138781683
3	19.893830451189967	5.111527008741334	54.519145010623085	20.475497529445622
4	19.203789612038264	4.053913394874391	50.76626984045994	25.976027152627402
5	18.40312998116215	5.193980868255468	52.085939780109435	24.316949370472944
6	20.949403127940226	7.092664667908917	54.23953662563703	17.718395578513828
7	83.92925295578718	1.8946019254200759	10.103863289868176	4.072281828924569
8	85.45485345051034	3.2683566986617576	6.582634482448961	4.6941553683789445
9	79.68185872225091	5.415626639127622	8.448663288235426	6.453851350386043
10	37.203222231519824	34.124672684709914	15.633782407530243	13.038322676240021
11	24.58696535101057	24.6518671513212	32.1306771217072	18.630490375961024
12	22.94564372177129	22.23172391835435	34.718177198242344	20.104455161632014
13	22.313769590445148	24.390831294096998	35.24147347496076	18.05392564049709
14	18.885811609258507	27.390600668202815	33.001136801974	20.722450920564686
15	17.087337822034364	26.009498521341058	37.01974198472971	19.883421671894865
16	20.12486453279888	25.113527127115688	32.77663371913848	21.984974620946954
17	20.166295556267617	24.613905720950832	31.527172016303005	23.69262670647855
18	20.10098556853365	23.888964857103787	33.61341793697994	22.396631637382622
19	21.483312277665412	24.86963514167165	31.79698390312896	21.850068677533976
20	23.61956115770117	27.048539607446152	31.11082084449896	18.221078390353714
21	23.88529117029375	27.087317412663197	30.72079776150017	18.30659365554288
22	21.7770031287566	23.771610972894315	31.491659710472657	22.959726187876424
23	20.403656542938258	26.182978176259414	30.733247477911956	22.680117802890376
24	21.528212894232514	25.01617442664974	31.235113914905167	22.220498764212575
25	19.465029562974134	27.154056056378845	29.651550806068116	23.729363574578905
26	18.49578852625972	27.195078892424245	31.621463311093922	22.687669270222116
27	18.357208896036703	27.769602690771496	32.30374858920222	21.569439823989583
28	16.25973782121799	26.57463400895155	35.12167046621126	22.043957703619192
29	17.829626651373246	23.964683624132856	33.80791924420017	24.397770480293733
30	19.455437158525708	25.642946215184164	32.70234360809109	22.199273018199037
31	21.457596469995163	26.660149274140714	29.57991391327242	22.3023403425917
32	20.64611987240061	28.352290237585493	28.663329054168514	22.338260835845386
33	20.71265442240459	26.93730853458674	28.645368807541672	23.704668235466997
34	19.95995681377061	25.553349075761627	30.994283335136164	23.492410775331603
35	18.111275973475983	26.48585324437569	30.688142767633188	24.714728014515146
36	19.21481067246837	26.48585324437569	32.7068336697478	21.59250241340814
37	18.700494519063373	26.49156786830241	33.28258203036506	21.525355582269153
38	17.97534956150466	28.064314010421025	29.70481926481363	24.25551716326068
39	20.410187541711654	26.56544979192646	29.11151884499287	23.91284382136902
40	20.921034102018282	25.54661398327656	29.656244961436496	23.876106953268664
41	18.971939155582675	27.745315539082927	28.911302913845923	24.371442391488475
42	19.05337254653847	27.650411963157	30.240565257943835	23.05565023236069
43	19.840970179867785	26.14154715279067	30.201583359015128	23.81589930832641
44	18.56905816874876	24.872288359923342	31.343487675801217	25.215165795526673
45	19.687083521269628	24.092650381349102	29.33030730390166	26.88995879347961
46	21.24329807274308	25.285782219764023	30.206277514383505	23.26464219310939
47	17.50552583724343	25.730502417490015	33.4542248418784	23.30974690338816
48	18.092907539425802	25.198021923746506	32.32905620944913	24.38001432737856
49	19.36441136312149	23.968153217231226	33.49504358421213	23.172391835435157
50	19.570137824483492	24.25306803872066	30.541603482655095	25.635190654140754
51	18.93805959944568	24.07122054162389	28.89068944896739	28.10003040996304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	174.0
2	283.0
3	608.0
4	933.0
5	719.5
6	506.0
7	556.0
8	606.0
9	711.0
10	816.0
11	911.0
12	1006.0
13	1068.0
14	1130.0
15	1282.0
16	1434.0
17	1422.5
18	1411.0
19	1454.5
20	1498.0
21	1954.0
22	2410.0
23	3100.0
24	3790.0
25	4330.5
26	5619.0
27	6367.0
28	7994.0
29	9621.0
30	10995.0
31	12369.0
32	13397.0
33	14425.0
34	15917.0
35	17409.0
36	18906.5
37	20404.0
38	22562.5
39	24721.0
40	27418.0
41	30115.0
42	33209.0
43	36303.0
44	43771.5
45	51240.0
46	52510.5
47	53781.0
48	51433.5
49	49086.0
50	41652.5
51	34219.0
52	30061.0
53	25903.0
54	22830.0
55	19757.0
56	17888.5
57	16020.0
58	14821.0
59	13622.0
60	12640.5
61	11659.0
62	9910.5
63	8162.0
64	7195.5
65	6229.0
66	4749.0
67	3269.0
68	2597.5
69	1926.0
70	1525.5
71	1125.0
72	914.5
73	704.0
74	554.0
75	318.5
76	233.0
77	152.0
78	71.0
79	64.0
80	57.0
81	31.5
82	6.0
83	4.0
84	2.0
85	1.0
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.74570797441344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62090791797002	26.68506675190495
2	10.769550343672698	7.9146950388868165
3	3.772048966895019	4.158198294081369
4	1.858838304636221	2.7321731805526537
5	1.3166513975915093	2.4190643880000464
6	0.9750671566220013	2.149771979560423
7	0.8439538099791797	2.1708176171771982
8	0.7573598798222604	2.226377998038851
9	0.7083503076833921	2.342595019474785
>10	6.256549745144427	39.656289126555514
>50	0.07748678817533239	2.011890542464392
>100	0.03762039715758878	2.604254171570658
>500	0.0016844953951159155	0.37427642744645645
>1k	0.003930489255270469	2.554529464285875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2602	0.5310518377618267	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	1882	0.3841043653603989	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1864	0.38043067855036317	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1722	0.35144937149341493	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	1554	0.3171616279330818	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1463	0.2985891001712346	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	1294	0.2640972628992328	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTTCCGAAT	687	0.1402123799163624	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	620	0.12653810123456286	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	507	0.10347551181600542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40818742333729957	0.0
2	0.0	0.0	0.0	1.7170403962683505	0.0
3	0.0	0.0	0.0	2.1809454028911914	0.0
4	0.0	0.0	0.0	2.9914015319273997	0.0
5	0.0	0.0	0.0	4.414138795969557	0.0
6	0.0	0.0	0.0	5.013357933428713	0.0
7	0.0	0.0	0.0	5.641966565368154	0.0
8	0.0	0.0	0.0	6.619575444260987	0.0
9	0.0	0.0	0.0	7.023885087076582	0.0
10	0.0	0.0	0.0	8.227017517363272	0.0
11	0.0	0.0	0.0	9.882829800131027	0.0
12	0.0	0.0	0.0	11.869273895801996	0.0
13	0.0	0.0	0.0	12.3946111096371	0.0
14	0.0	0.0	0.0	12.563804796610412	0.0
15	0.0	0.0	0.0	12.90423310767372	0.0
16	0.0	0.0	0.0	13.568558139155174	0.0
17	0.0	0.0	0.0	14.619436660537053	0.0
18	0.0	0.0	0.0	15.812976686375317	0.0
19	0.0	0.0	0.0	16.740582605909328	0.0
20	0.0	0.0	0.0	17.55348785948556	0.0
21	0.0	0.0	0.0	18.55293476552694	0.0
22	0.0	0.0	0.0	19.658918589059354	0.0
23	0.0	0.0	0.0	20.686122239887666	0.0
24	0.0	0.0	0.0	21.478414028585366	0.0
25	0.0	0.0	0.0	22.176618616203815	0.0
26	0.0	0.0	0.0	22.807064091548277	0.0
27	0.0	0.0	0.0	23.4442446593778	0.0
28	0.0	0.0	0.0	24.11101881539928	0.0
29	0.0	0.0	0.0	24.811264340134414	0.0
30	0.0	0.0	0.0	25.61457718926222	0.0
31	0.0	0.0	0.0	26.37360170295793	0.0
32	0.0	0.0	0.0	27.06915307232469	0.0
33	0.0	0.0	0.0	27.73225354153613	0.0
34	0.0	0.0	0.0	28.415967475626108	0.0
35	0.0	0.0	0.0	29.158664492388326	0.0
36	0.0	0.0	0.0	29.84870533154003	0.0
37	0.0	0.0	0.0	30.576707601062104	0.0
38	0.0	0.0	0.0	31.31634321214929	0.0
39	0.0	0.0	0.0	32.00413902047264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGG	30	2.162942E-6	45.000004	2
CCGATCG	20	7.029319E-4	45.000004	40
CTACGGG	45	3.8380676E-10	45.000004	3
CTACGGC	45	3.8380676E-10	45.000004	6
CTCGATT	45	3.8380676E-10	45.000004	24
TTCGCAG	25	3.8875172E-5	45.000004	1
CGATCGA	20	7.029319E-4	45.000004	41
TATCGGG	30	2.162942E-6	45.000004	3
TATCGAC	25	3.8875172E-5	45.000004	15
TTCGGGC	110	0.0	40.909092	4
CCGATGA	160	0.0	40.781254	18
ATCTCGA	50	1.0786607E-9	40.500004	22
TCTCGAT	50	1.0786607E-9	40.500004	23
CTTGTCG	95	0.0	40.263157	1
TTCGGGA	455	0.0	40.054943	4
TCTTGCG	85	0.0	39.705883	1
TTCCGCG	85	0.0	39.705883	1
GCACGGG	75	0.0	39.0	3
CTGCTCG	35	6.2409526E-6	38.57143	1
CCGGGTC	35	6.2409526E-6	38.57143	5
>>END_MODULE