##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934032.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 504931 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.06326408954887 31.0 30.0 31.0 26.0 34.0 2 30.42112090562869 31.0 30.0 33.0 27.0 34.0 3 30.42338062032238 31.0 30.0 34.0 26.0 34.0 4 34.01664781920698 35.0 33.0 37.0 30.0 37.0 5 34.417758069914505 35.0 35.0 37.0 32.0 37.0 6 33.95857255743854 35.0 35.0 37.0 30.0 37.0 7 34.35788058170324 35.0 35.0 37.0 32.0 37.0 8 34.55973588470504 35.0 35.0 37.0 32.0 37.0 9 35.85530102132767 37.0 35.0 39.0 30.0 39.0 10 35.286482707538255 37.0 34.0 39.0 30.0 39.0 11 35.46053223113653 37.0 35.0 39.0 30.0 39.0 12 35.46260182084285 37.0 35.0 39.0 30.0 39.0 13 35.45192313405198 37.0 35.0 39.0 30.0 39.0 14 36.13210517872739 38.0 35.0 40.0 29.0 41.0 15 36.42660878417051 38.0 35.0 40.0 30.0 41.0 16 36.54846107686001 38.0 35.0 40.0 31.0 41.0 17 36.363095155575714 38.0 35.0 40.0 30.0 41.0 18 36.44754629840513 38.0 35.0 40.0 30.0 41.0 19 36.45721494620057 38.0 35.0 40.0 30.0 41.0 20 36.51065195046452 38.0 35.0 40.0 30.0 41.0 21 36.17447532435125 38.0 34.0 40.0 30.0 41.0 22 36.360377952631154 38.0 35.0 40.0 30.0 41.0 23 36.447982001501195 38.0 35.0 40.0 30.0 41.0 24 36.37098336208314 38.0 35.0 40.0 30.0 41.0 25 35.86300702472219 38.0 34.0 40.0 29.0 41.0 26 35.97281410727406 38.0 34.0 40.0 30.0 41.0 27 36.045525032133106 38.0 34.0 40.0 30.0 41.0 28 35.91535675171459 38.0 34.0 40.0 29.0 41.0 29 36.038096294345166 38.0 34.0 40.0 30.0 41.0 30 35.575361782104885 38.0 34.0 40.0 28.0 41.0 31 35.753293024195386 38.0 34.0 40.0 29.0 41.0 32 35.806771618300324 38.0 34.0 40.0 29.0 41.0 33 35.82537217956513 38.0 34.0 40.0 29.0 41.0 34 35.7877195101905 38.0 34.0 40.0 29.0 41.0 35 35.78443985415829 38.0 34.0 40.0 29.0 41.0 36 35.54853633466751 38.0 34.0 40.0 28.0 41.0 37 35.64713396483876 38.0 34.0 40.0 29.0 41.0 38 35.532312335744884 38.0 34.0 40.0 29.0 41.0 39 35.43034394798497 38.0 34.0 40.0 28.0 41.0 40 35.338911653275396 38.0 34.0 40.0 28.0 41.0 41 35.18924565930791 37.0 34.0 40.0 27.0 41.0 42 35.403379867744306 37.0 34.0 40.0 28.0 41.0 43 35.35175499226627 37.0 34.0 40.0 28.0 41.0 44 35.32349172461188 37.0 34.0 40.0 28.0 41.0 45 35.39348346605774 37.0 34.0 40.0 28.0 41.0 46 35.25598745175083 37.0 34.0 40.0 28.0 41.0 47 35.20655495503346 37.0 34.0 40.0 28.0 41.0 48 35.1677734185463 37.0 34.0 40.0 28.0 41.0 49 35.13726627994716 37.0 34.0 40.0 28.0 41.0 50 34.94953964006963 36.0 33.0 40.0 27.0 41.0 51 33.82192022276311 35.0 32.0 39.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 8.0 11 15.0 12 13.0 13 21.0 14 25.0 15 23.0 16 55.0 17 134.0 18 243.0 19 407.0 20 669.0 21 1114.0 22 1689.0 23 2321.0 24 3228.0 25 4534.0 26 5771.0 27 7485.0 28 9664.0 29 12311.0 30 15529.0 31 19733.0 32 25206.0 33 33296.0 34 45604.0 35 48673.0 36 58392.0 37 74924.0 38 89835.0 39 44004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.88260772263933 4.718466483539335 53.43185504554088 16.96707074828046 2 20.74243807569747 4.2401833121753265 53.48295113589777 21.534427476229425 3 22.65061958960729 4.367527444343881 51.42900713166749 21.552845834381333 4 20.31723146330885 5.353008628901771 46.80976212591423 27.519997781875148 5 18.696415945941126 6.322646064511785 48.86430027073006 26.116637718817024 6 22.35790632779528 6.962931568867825 52.384385193224425 18.29477691011247 7 86.10166537606129 2.637588106097665 7.393485446526357 3.8672610713146947 8 87.61137660393203 3.3174829828233956 5.2274469184898535 3.8436934947547288 9 81.42538287409566 5.876446484767225 7.376849510131087 5.321321131006019 10 40.70774026550163 29.489969916681684 14.89252987041794 14.909759947398754 11 31.981399438735192 25.79421742772775 23.7040308477792 18.52035228575786 12 29.44838007569351 22.955413709992058 27.849745806852816 19.746460407461612 13 25.252559260572237 24.30648940152219 29.053276586305856 21.38767475159972 14 21.35638334742767 26.44341504086697 30.201156197579472 21.999045414125888 15 19.803497903673968 24.76377960553026 34.30151842528979 21.13120406550598 16 22.84628988911356 23.385769540788743 32.24004864030927 21.527891929788424 17 22.975020349315056 22.859757075719255 29.28598164897778 24.879240925987908 18 24.004864030927 23.17861252329526 30.008456601000926 22.80806684477681 19 25.976618587490176 25.07491122549418 27.185496632213113 21.762973554802535 20 28.63539770780562 25.495166666336587 26.554519330363952 19.314916295493838 21 27.26471537695249 25.26563035345423 27.05557789084053 20.41407637875274 22 25.222654184433118 23.773149202564312 27.020325549431508 23.983871063571062 23 24.373627287688812 25.981173665312685 27.30531498363143 22.339884063367073 24 24.22311167268399 23.93435934810895 29.332324614650318 22.510204364556742 25 23.971988251860154 25.55002564706861 26.740485333639647 23.73750076743159 26 23.417258991822646 27.05419156280759 26.029299052741862 23.499250392627903 27 23.063547296561314 26.448960352998725 28.016105170805517 22.471387179634444 28 20.977321653849735 26.809603688424755 29.373914455638495 22.839160202087015 29 22.876194965252676 25.792435005971114 28.18840594061367 23.14296408816254 30 23.69016756744981 25.475361980151746 28.006202827713096 22.828267624685353 31 24.599796803919745 26.46678457056509 26.196054510418254 22.737364115096913 32 25.642711578413685 26.79019509596361 26.17763615226635 21.38945717335636 33 25.206018248037847 26.418857229997762 26.100001782421757 22.27512273954263 34 23.560248826077228 25.624689313985478 28.043435637740604 22.771626222196698 35 24.03694762254645 25.74034868130497 27.13539077616546 23.087312919983127 36 23.746016782491072 27.333635684875755 26.231504898689128 22.688842633944045 37 23.17267111743981 27.79706534160113 26.761280254133734 22.26898328682533 38 23.646003117257607 26.567590423245946 26.823268921892296 22.963137537604148 39 23.574508200130314 25.822736175833928 27.055379843978685 23.547375780057077 40 25.76451039845048 24.657032346993944 27.552873560941993 22.025583693613584 41 21.932105574821115 25.289594023737898 28.354171163980823 24.424129237460168 42 23.423794538263646 24.82913506994025 27.795084872982645 23.951985518813462 43 22.905109807082553 24.91508740798248 28.169591488738067 24.010211296196907 44 23.16375900865663 25.693609621908735 27.194606787858145 23.948024581576494 45 23.912376146443773 25.113926457278325 26.180804902055925 24.79289249422198 46 23.10216643462176 26.124163499567267 26.70959002319129 24.064080042619686 47 21.85249073635804 26.595118937042884 28.783338713606415 22.769051612992666 48 22.75459419207773 25.271967853033384 28.15196531803355 23.821472636855333 49 22.776973487466602 24.844384678302582 29.125365644018686 23.253276190212127 50 21.985776274382044 25.64053306293335 27.85113213488576 24.52255852779885 51 21.973101275223744 25.188986217918885 26.81277243821433 26.025140068643044 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 94.0 1 243.0 2 392.0 3 642.5 4 893.0 5 718.0 6 543.0 7 574.5 8 606.0 9 622.5 10 639.0 11 673.5 12 708.0 13 711.0 14 714.0 15 709.5 16 705.0 17 858.0 18 1011.0 19 1117.5 20 1224.0 21 1510.5 22 1797.0 23 2054.0 24 2311.0 25 2582.5 26 3729.5 27 4605.0 28 5548.5 29 6492.0 30 7872.5 31 9253.0 32 10612.0 33 11971.0 34 13367.5 35 14764.0 36 16311.0 37 17858.0 38 19515.5 39 21173.0 40 22335.0 41 23497.0 42 25477.5 43 27458.0 44 28528.5 45 29599.0 46 30860.5 47 32122.0 48 34795.5 49 37469.0 50 39112.5 51 40756.0 52 40935.0 53 41114.0 54 37990.0 55 34866.0 56 32315.5 57 29765.0 58 28923.0 59 28081.0 60 25849.5 61 23618.0 62 21079.5 63 18541.0 64 15676.5 65 12812.0 66 10956.0 67 9100.0 68 7461.5 69 5823.0 70 4953.5 71 4084.0 72 3332.0 73 2580.0 74 2055.0 75 1141.5 76 753.0 77 600.5 78 448.0 79 335.0 80 222.0 81 146.0 82 70.0 83 40.5 84 11.0 85 5.5 86 0.0 87 1.5 88 3.0 89 1.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 504931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.934749147588036 #Duplication Level Percentage of deduplicated Percentage of total 1 73.88857414030717 24.335016541839774 2 8.219444366286643 5.414106766724127 3 2.8861137406274224 2.8516029617691343 4 1.558828106867508 2.0535845065556377 5 1.2100627064027742 1.9926555844113423 6 0.985707135272073 1.9478410339904013 7 0.924959870464295 2.132432491372896 8 0.7942975931844396 2.092799358404996 9 0.7837038030563194 2.3229979343703553 >10 8.630014398036064 50.75191710807222 >50 0.08963525002991035 2.0072370185572606 >100 0.025610071437117243 1.4362940627035043 >500 0.003048818028228243 0.6615146312283757 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 890 0.176261707045121 No Hit CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT 684 0.1354640535043402 Illumina Single End Adapter 1 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 630 0.12476952296452387 No Hit TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 586 0.11605546104319203 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1522980367614585 0.0 2 0.0 0.0 0.0 0.632957770467648 0.0 3 0.0 0.0 0.0 0.831598772901644 0.0 4 0.0 0.0 0.0 1.1490678924447102 0.0 5 0.0 0.0 0.0 1.6843885600210722 0.0 6 0.0 0.0 0.0 1.9953221331231397 0.0 7 0.0 0.0 0.0 2.2933826602050575 0.0 8 0.0 0.0 0.0 2.7865193462076996 0.0 9 0.0 0.0 0.0 3.0202146431888712 0.0 10 0.0 0.0 0.0 3.519094688185118 0.0 11 0.0 0.0 0.0 4.356436820080368 0.0 12 0.0 0.0 0.0 5.201898873311403 0.0 13 0.0 0.0 0.0 5.463320730951358 0.0 14 0.0 0.0 0.0 5.55759103719122 0.0 15 0.0 0.0 0.0 5.7340507910981895 0.0 16 0.0 0.0 0.0 6.0970706888664 0.0 17 0.0 0.0 0.0 6.629222606653186 0.0 18 0.0 0.0 0.0 7.216827645757539 0.0 19 0.0 0.0 0.0 7.6087623853556225 0.0 20 0.0 0.0 0.0 8.03218657598761 0.0 21 0.0 0.0 0.0 8.607908803381056 0.0 22 0.0 0.0 0.0 9.222448215696797 0.0 23 0.0 0.0 0.0 9.819955597893573 0.0 24 0.0 0.0 0.0 10.302991893941945 0.0 25 0.0 0.0 0.0 10.73295163101493 0.0 26 0.0 0.0 0.0 11.104487543842623 0.0 27 0.0 0.0 0.0 11.476023456670317 0.0 28 0.0 0.0 0.0 11.892912100861306 0.0 29 0.0 0.0 0.0 12.34109214922435 0.0 30 0.0 0.0 0.0 12.843933131457565 0.0 31 0.0 0.0 0.0 13.29448974216279 0.0 32 0.0 0.0 0.0 13.742471743663986 0.0 33 0.0 0.0 0.0 14.198573666500968 0.0 34 0.0 0.0 0.0 14.654873636199797 0.0 35 0.0 0.0 0.0 15.159497040189649 0.0 36 0.0 0.0 0.0 15.636988024106264 0.0 37 0.0 0.0 0.0 16.111508305095153 0.0 38 0.0 0.0 0.0 16.642868035434546 0.0 39 0.0 0.0 0.0 17.273251196698162 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAG 25 3.8876875E-5 45.0 1 ACCGGTA 30 2.163075E-6 44.999996 41 TTGACGA 30 2.163075E-6 44.999996 14 TTGTTCG 75 0.0 42.0 1 CGGGACG 55 6.002665E-11 40.909092 6 CGCGGGT 50 1.0786607E-9 40.5 4 ACGGGAC 50 1.0786607E-9 40.5 5 TCGTTTG 185 0.0 40.135136 1 TTTAGCG 45 1.9246727E-8 40.0 1 TCGTACA 45 1.9246727E-8 40.0 34 TTGGGAC 825 0.0 39.81818 5 GCGATCT 125 0.0 39.600002 9 TTTGGGC 1060 0.0 39.481133 4 TCGATCA 35 6.241331E-6 38.57143 17 TCGGATG 35 6.241331E-6 38.57143 5 CTCGATC 35 6.241331E-6 38.57143 16 CGATGCG 70 0.0 38.57143 10 TTTGGGA 2610 0.0 37.84483 4 GGCACCG 220 0.0 37.84091 8 CTACGAA 125 0.0 37.800003 11 >>END_MODULE