##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934031.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278645
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.106066859265372	31.0	30.0	31.0	26.0	34.0
2	30.404941771788476	31.0	30.0	33.0	27.0	34.0
3	30.423097489637353	31.0	30.0	34.0	26.0	34.0
4	34.11137827701915	35.0	33.0	37.0	30.0	37.0
5	34.49507078899675	35.0	35.0	37.0	32.0	37.0
6	34.04082972958424	35.0	35.0	37.0	30.0	37.0
7	34.513599741606704	35.0	35.0	37.0	32.0	37.0
8	34.70456315383373	35.0	35.0	37.0	32.0	37.0
9	36.04151160078236	37.0	35.0	39.0	31.0	39.0
10	35.267627267670335	37.0	34.0	39.0	30.0	39.0
11	35.438977910961974	37.0	35.0	39.0	30.0	39.0
12	35.4152164941054	37.0	35.0	39.0	30.0	39.0
13	35.35316262628075	37.0	35.0	39.0	30.0	39.0
14	36.024231549103696	38.0	34.0	40.0	29.0	41.0
15	36.339227332268656	38.0	35.0	40.0	30.0	41.0
16	36.48066536273753	38.0	35.0	40.0	31.0	41.0
17	36.2677385203395	38.0	35.0	40.0	30.0	41.0
18	36.389352042922	38.0	35.0	40.0	30.0	41.0
19	36.38513879667678	38.0	35.0	40.0	30.0	41.0
20	36.45175043514149	38.0	35.0	40.0	30.0	41.0
21	36.107057366900534	38.0	34.0	40.0	30.0	41.0
22	36.2989466884387	38.0	34.0	40.0	30.0	41.0
23	36.398402985878086	38.0	35.0	40.0	30.0	41.0
24	36.31032317105995	38.0	35.0	40.0	30.0	41.0
25	35.80708787166466	38.0	34.0	40.0	29.0	41.0
26	35.90680973999174	38.0	34.0	40.0	30.0	41.0
27	35.95499291212833	38.0	34.0	40.0	30.0	41.0
28	35.887193382260584	38.0	34.0	40.0	30.0	41.0
29	36.00638087889609	38.0	34.0	40.0	30.0	41.0
30	35.519104954332576	38.0	34.0	40.0	29.0	41.0
31	35.71591092608875	38.0	34.0	40.0	29.0	41.0
32	35.75770245294191	38.0	34.0	40.0	29.0	41.0
33	35.779235227619374	38.0	34.0	40.0	29.0	41.0
34	35.76898203807713	38.0	34.0	40.0	29.0	41.0
35	35.76924402016903	38.0	34.0	40.0	29.0	41.0
36	35.5392560426349	38.0	34.0	40.0	29.0	41.0
37	35.62791365357354	38.0	34.0	40.0	29.0	41.0
38	35.543706149401565	38.0	34.0	40.0	29.0	41.0
39	35.51288557124657	38.0	34.0	40.0	29.0	41.0
40	35.45681781478225	38.0	34.0	40.0	29.0	41.0
41	35.38476197311992	38.0	34.0	40.0	28.0	41.0
42	35.62229359938273	38.0	34.0	40.0	29.0	41.0
43	35.585698648818386	38.0	34.0	40.0	29.0	41.0
44	35.547316477955825	38.0	34.0	40.0	29.0	41.0
45	35.599867214556156	38.0	34.0	40.0	29.0	41.0
46	35.46826966211488	37.0	34.0	40.0	29.0	41.0
47	35.41554666331712	37.0	34.0	40.0	28.0	41.0
48	35.42566706741553	37.0	34.0	40.0	28.0	41.0
49	35.438296039763856	37.0	34.0	40.0	28.0	41.0
50	35.28558560175133	37.0	34.0	40.0	28.0	41.0
51	34.15777064006173	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	5.0
12	4.0
13	4.0
14	6.0
15	17.0
16	26.0
17	46.0
18	112.0
19	179.0
20	287.0
21	540.0
22	801.0
23	1151.0
24	1573.0
25	2327.0
26	2932.0
27	3895.0
28	5053.0
29	6456.0
30	8503.0
31	11077.0
32	14115.0
33	18864.0
34	26469.0
35	27456.0
36	32639.0
37	41870.0
38	49300.0
39	22931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.391573507509555	4.883992176425201	57.658310753826555	15.06612356223869
2	19.52233128173841	4.687685047282384	55.459096700102286	20.33088697087692
3	20.261623212331102	4.6869672881264695	53.843779719715045	21.20762977982738
4	19.195391986219022	4.412424410988892	50.57474564409912	25.817437958692963
5	17.77602325539665	5.43056577365465	52.56724506091981	24.22616591002889
6	20.952107520321555	6.807586714278024	54.79983491539414	17.44047085000628
7	84.00724936747474	2.0355649661756	9.752193651420265	4.20499201492939
8	86.3607816397208	3.1086149042688724	5.88526619892695	4.645337257083386
9	81.40178363150244	4.40237578280608	8.088786807586715	6.107053778104757
10	41.48145489780904	24.325934432701107	17.54311040930216	16.649500260187693
11	31.103375262430692	25.589908306267834	23.112203700048447	20.194512731253027
12	26.107053778104756	22.48416443862262	30.395305855120313	21.01347592815231
13	22.626998510649752	27.222092626819073	31.34023578388272	18.81067307864846
14	19.282599723662724	28.61346875056075	31.810009151429234	20.293922374347286
15	18.056308205781548	24.845592061583734	37.046779952986775	20.05131977964794
16	19.691004683378495	24.42031976170396	32.97277898401191	22.915896570905634
17	19.6511690502252	24.854564051032675	30.21299502951785	25.28127186922428
18	21.72800516786592	23.94623983922195	31.426366882592543	22.89938811031958
19	22.95393780616914	27.353801431929515	28.0220352060866	21.670225555814746
20	26.105259380214967	26.67085359507617	28.226237685944483	18.997649338764376
21	24.400222505338334	28.42075041719751	27.43203717992428	19.74698989753988
22	22.907642340612608	24.952897055393063	28.183889895745484	23.955570708248846
23	21.39281164205351	27.129501695706004	27.12591289992643	24.351773762314053
24	22.45904286816559	25.331873889716306	29.65098961043622	22.55809363168189
25	20.27131296093596	27.89355631717777	27.781944768432954	24.05318595345332
26	19.174576970697483	29.77839186061117	28.281864020527912	22.765167148163435
27	20.222146458755766	29.430637549570243	28.23341527750363	22.11380071417036
28	19.106389850885535	28.230185361302013	30.770335014086026	21.893089773726427
29	20.45290602738251	26.819429740350625	30.060471208885858	22.667193023381003
30	21.747384665075632	28.711083995765218	28.428645767912574	21.11288557124657
31	24.41242441098889	28.837050727628345	25.02646736887437	21.72405749250839
32	23.40827935186348	29.97254571228624	24.67763641909957	21.941538516750704
33	22.344919162375064	28.880475156561214	26.106336018948838	22.668269662114877
34	20.81429776238583	28.306985590984944	27.50273645678193	23.375980189847297
35	20.8010192180014	28.647921190044677	26.791078253691975	23.759981338261944
36	22.039871521111092	30.96341222702722	26.285058048771738	20.711658203089954
37	22.419924994168206	30.275798955660427	26.592617847081414	20.711658203089954
38	22.4902653914479	29.486263884153672	24.995962604747977	23.02750811965045
39	22.506056092878033	27.44065028979526	27.43742037359364	22.615873243733063
40	22.39229126666547	26.974824597606272	26.354321807317554	24.2785623284107
41	18.628362253045992	27.336575212187554	27.119094187945237	26.915968346821224
42	20.579949397979508	26.591900087925495	27.343752803746703	25.484397710348293
43	21.703960236142763	27.003534963842885	27.715552046510794	23.576952753503562
44	21.825979292648352	26.890487896786237	26.78425954171078	24.499273268854633
45	22.13784564589352	27.40153241579788	25.03938703368085	25.421234904627752
46	22.717436164295073	28.858224622727846	26.12427999784672	22.300059215130364
47	19.828096682158304	28.379838145310345	28.883346193184877	22.908718979346478
48	21.168511905829998	26.421791167973584	28.358305370632884	24.05139155556353
49	21.790809094008505	25.486550987816038	29.946706382673295	22.775933535502162
50	22.19311310089899	25.118340540831525	27.581689963932604	25.10685639433688
51	20.461519137253497	25.40077876868417	26.183495128209728	27.954206965852606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	100.0
2	154.0
3	319.0
4	484.0
5	360.0
6	236.0
7	290.0
8	344.0
9	404.5
10	465.0
11	540.0
12	615.0
13	642.0
14	669.0
15	687.5
16	706.0
17	689.0
18	672.0
19	728.0
20	784.0
21	955.0
22	1126.0
23	1214.0
24	1302.0
25	1547.0
26	2287.5
27	2783.0
28	3224.5
29	3666.0
30	4089.5
31	4513.0
32	5500.0
33	6487.0
34	6975.0
35	7463.0
36	8366.0
37	9269.0
38	10759.0
39	12249.0
40	13637.0
41	15025.0
42	17174.0
43	19323.0
44	20682.0
45	22041.0
46	25369.5
47	28698.0
48	29613.5
49	30529.0
50	28904.0
51	27279.0
52	23689.5
53	20100.0
54	17710.0
55	15320.0
56	13550.5
57	11781.0
58	10742.5
59	9704.0
60	8558.0
61	7412.0
62	6760.5
63	6109.0
64	4981.0
65	3853.0
66	2982.5
67	2112.0
68	1705.0
69	1298.0
70	1064.5
71	831.0
72	768.5
73	706.0
74	524.0
75	255.5
76	169.0
77	145.5
78	122.0
79	74.0
80	26.0
81	22.5
82	19.0
83	20.0
84	21.0
85	10.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	278645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.70613886373036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88285804669748	27.104250274305926
2	11.34091291903924	8.552440747335343
3	3.956821074631574	4.475893346969787
4	1.9945064859105948	3.00820554089423
5	1.3922040870789	2.624732031702498
6	1.0199174332432857	2.3074289020446455
7	0.8622356036828525	2.275810278400261
8	0.7764107791010058	2.342036212166368
9	0.7245169448266142	2.4586862877682183
>10	5.914892402044108	38.14576097944588
>50	0.08782023473704538	2.2912367058204746
>100	0.040916245729759784	2.492928752842937
>500	0.004989786064604851	1.4946283783082486
>1k	9.979572129209702E-4	0.4259615619952007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1132	0.4062516822480217	No Hit
TCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	943	0.33842344201403224	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	931	0.3341168870785408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGC	776	0.2784905524951103	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	664	0.23829603976385721	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	658	0.23614276229611156	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC	470	0.16867340164007968	No Hit
TGCCGTGGGCTGTTCTCCAGCGCAGACTTGAATCATACTCTCAGATTGACT	305	0.10945827127707297	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCC	299	0.10730499380932727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGGAGCTT	287	0.10299843887383589	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36821044698451433	0.0
2	0.0	0.0	0.0	1.4782249816074216	0.0
3	0.0	0.0	0.0	1.9286188519442302	0.0
4	0.0	0.0	0.0	2.7055931382224694	0.0
5	0.0	0.0	0.0	3.93260241525956	0.0
6	0.0	0.0	0.0	4.562794954153134	0.0
7	0.0	0.0	0.0	5.156022896517074	0.0
8	0.0	0.0	0.0	6.173446500026916	0.0
9	0.0	0.0	0.0	6.585799135100217	0.0
10	0.0	0.0	0.0	7.680740727448905	0.0
11	0.0	0.0	0.0	9.357426115666888	0.0
12	0.0	0.0	0.0	11.092967754669921	0.0
13	0.0	0.0	0.0	11.61262538355255	0.0
14	0.0	0.0	0.0	11.772326795743687	0.0
15	0.0	0.0	0.0	12.072709002494213	0.0
16	0.0	0.0	0.0	12.739507258339465	0.0
17	0.0	0.0	0.0	13.825117981661254	0.0
18	0.0	0.0	0.0	15.10524143623607	0.0
19	0.0	0.0	0.0	15.992750632525256	0.0
20	0.0	0.0	0.0	16.790539934325036	0.0
21	0.0	0.0	0.0	17.895530154856537	0.0
22	0.0	0.0	0.0	19.023847547955285	0.0
23	0.0	0.0	0.0	20.178363150244934	0.0
24	0.0	0.0	0.0	21.087764000789534	0.0
25	0.0	0.0	0.0	21.86258500960003	0.0
26	0.0	0.0	0.0	22.530101024601194	0.0
27	0.0	0.0	0.0	23.219149814279817	0.0
28	0.0	0.0	0.0	23.910710761004147	0.0
29	0.0	0.0	0.0	24.62524000071776	0.0
30	0.0	0.0	0.0	25.401496527840084	0.0
31	0.0	0.0	0.0	26.152631484505374	0.0
32	0.0	0.0	0.0	26.85316442067864	0.0
33	0.0	0.0	0.0	27.614348005526747	0.0
34	0.0	0.0	0.0	28.340002512157046	0.0
35	0.0	0.0	0.0	29.08611315473093	0.0
36	0.0	0.0	0.0	29.835453713506432	0.0
37	0.0	0.0	0.0	30.56326149760448	0.0
38	0.0	0.0	0.0	31.343465700084337	0.0
39	0.0	0.0	0.0	32.13587180821475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1595624E-6	45.000004	1
CGGCGAA	20	7.0240756E-4	45.000004	31
ACCGGCA	20	7.0240756E-4	45.000004	26
CTCAACG	20	7.0240756E-4	45.000004	18
TTTACGG	20	7.0240756E-4	45.000004	2
TTTCGCA	20	7.0240756E-4	45.000004	13
TGGCGTA	20	7.0240756E-4	45.000004	12
GGCGTAC	20	7.0240756E-4	45.000004	13
TTCACGG	20	7.0240756E-4	45.000004	2
TTATCGG	40	6.7830115E-9	45.000004	2
ACGGGGC	35	1.2074997E-7	45.000004	5
AGAACGC	20	7.0240756E-4	45.000004	34
AACGGGT	20	7.0240756E-4	45.000004	19
AGCGCGA	20	7.0240756E-4	45.000004	13
TTCGGTG	20	7.0240756E-4	45.000004	1
CTCGAAT	20	7.0240756E-4	45.000004	37
GGATTCG	35	1.2074997E-7	45.000004	8
TGGGACG	30	2.1595624E-6	45.000004	6
CCAAACG	20	7.0240756E-4	45.000004	33
GCGTACC	20	7.0240756E-4	45.000004	14
>>END_MODULE